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PR01751

Identifier
AP2CTNSCPFCT  [View Relations]  [View Alignment]  
Accession
PR01751
No. of Motifs
4
Creation Date
22-MAY-2002
Title
Transcription factor AP-2 gamma signature
Database References
PRINTS; PR01748 AP2TNSCPFCT
MIM; 601602
Literature References
1. WILLIAMS, T. AND TJIAN, R.
Analysis of the DNA-binding and activation properties of the human
transcription factor AP-2.
GENES DEV. 5 670-682 (1991).
 
2. WILLIAMS, T. AND TJIAN, R.
Characterization of a dimerization motif in AP-2 and its function in
heterologous DNA-binding proteins.
SCIENCE 251 1067-1071 (1991).
 
3. HILGER-EVERSHEIM, K., MOSER, M., SCHORLE, H. AND BUETTNER, R. 
Regulation roles of AP-2 transcription factors in vertebrate development, 
apoptosis and cell-cycle control, 
GENE 260 1-12 (2000).
 
4. JEAN, D., GERSHENWALD, J., HUANG, S., LUCA, M., HUDSON, M., TAINSKY, M.
AND BAR-ELI, M.
Loss of AP-2 results in up-regulation of MCAM/MUC18 and an increase in tumor
growth and metastasis of human melanoma cells.
J.BIOL.CHEM. 273 16501-16508 (1998).
 
5. ANTTILA, M., KELLOKOSKI, J., MOISIO, K., MITCHELL, P., SAARIKOSKI, S., 
SYRJANEN, K. AND KOSMA, V.
Expression of transcription factor AP-2alpha predicts survival in epithelial
ovarian cancer.
BR.J.CANCER 82 1974-1983 (2000).
 
6. CHAZAUD, C., OULAD-ABDELGHANI, M., BOUILLET, P., DECIMO, D., CHAMBON, P.
AND DOLLE, P.
AP-2.2, a novel gene related to AP-2, is expressed in the forebrain, limbs 
and face during mouse embryogenesis.
MECH.DEV. 54 83-94 (1996).

Documentation
Activator protein-2 (AP-2) transcription factors constitute a family of
closely related and evolutionarily conserved proteins that bind to the DNA 
consensus sequence GCCNNNGGC and stimulate target gene transcription [1,2].
Four different isoforms of AP-2 have been identifed in mammals, termed AP-2
alpha, beta, gamma and delta. Each family member shares a common structure, 
possessing a proline/glutamine-rich domain in the N-terminal region, which 
is responsible for transcriptional activation [1], and a helix-span-helix
domain in the C-terminal region, which mediates dimerisation and site-
specific DNA binding [2].
 
The AP-2 family have been shown to be critical regulators of gene expression
during embryogenesis. They regulate the development of facial prominence and
limb buds, and are essential for cranial closure and development of the lens
[3]; they have also been implicated in tumorigenesis. AP-2 protein 
expression levels have been found to affect cell transformation, tumour 
growth and metastasis, and may predict survival in some types of cancer [4,5].
 
AP-2 gamma was originally isolated from murine carcinoma cells [6]. The 
gene was found to be expressed in several embryonic areas whose development 
can be affected by retinoids, such as the forebrain, face and limb buds [6].
A human homologue has also been identified. The protein was initially termed
AP-2.2, but has since been reclassified as AP-2 gamma.
 
AP2CTNSCPFCT is a 4-element fingerprint that provides a signature for the
transcription factor AP-2 gamma proteins. The fingerprint was derived from
an initial alignment of 3 sequences: the motifs were drawn from conserved 
regions in the N-terminal half of the alignment, focusing on those sections
which characterise AP-2 gamma but distinguish it from other family members -
motifs 1 and 2 lie in the transcriptional activation domain; and motifs 3 
and 4 reside between the transcriptional activation domain and the 
dimerisation domain. A single iteration on SPTR40_20f was required to reach
convergence, no further sequences being identified beyond the starting set.
Summary Information
3 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
43333
30000
20000
1234
True Positives
AP2C_HUMAN    AP2C_MOUSE    Q99L72        
Sequence Titles
AP2C_HUMAN  Transcription factor AP-2 gamma (AP2-gamma) (Activating enhancer- binding protein 2 gamma) (Transcription factor ERF-1) - Homo sapiens (Human). 
AP2C_MOUSE Transcription factor AP-2 gamma (AP2-gamma) (Activating enhancer- binding protein 2 gamma) (AP-2.2) - Mus musculus (Mouse).
Q99L72 TRANSCRIPTION FACTOR AP-2, GAMMA - Mus musculus (Mouse).
Scan History
SPTR40_20f 1  300  NSINGLE    
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
QQLAYSQSAD Q99L72 65 65 -
QQVVYSQSAD AP2C_MOUSE 65 65 -
QQLAYSQSAD AP2C_HUMAN 65 65 -

Motif 2 width=11
Element Seqn Id St Int Rpt
HLGEAYAAAMN Q99L72 78 3 -
HLGEAYAAAMN AP2C_MOUSE 78 3 -
HLGEAYAAAIN AP2C_HUMAN 78 3 -

Motif 3 width=14
Element Seqn Id St Int Rpt
LASHHSRSASLIPH Q99L72 114 25 -
LASHHSRSASLIPH AP2C_MOUSE 114 25 -
LPSHHGRPAGLLPH AP2C_HUMAN 114 25 -

Motif 4 width=19
Element Seqn Id St Int Rpt
NPLGLPCQKDLVGVVMNPS Q99L72 202 74 -
NPLGLPCQKDLVGVVMNPS AP2C_MOUSE 202 74 -
NPLNLPCQKELVGAVMNPT AP2C_HUMAN 203 75 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
QQLAYSQSAD Q99L72 65 65 -
QQVVYSQSAD AP2C_MOUSE 65 65 -
QQLAYSQSAD AP2C_HUMAN 65 65 -

Motif 2 width=11
Element Seqn Id St Int Rpt
HLGEAYAAAMN Q99L72 78 3 -
HLGEAYAAAMN AP2C_MOUSE 78 3 -
HLGEAYAAAIN AP2C_HUMAN 78 3 -

Motif 3 width=14
Element Seqn Id St Int Rpt
LASHHSRSASLIPH Q99L72 114 25 -
LASHHSRSASLIPH AP2C_MOUSE 114 25 -
LPSHHGRPAGLLPH AP2C_HUMAN 114 25 -

Motif 4 width=19
Element Seqn Id St Int Rpt
NPLGLPCQKDLVGVVMNPS Q99L72 202 74 -
NPLGLPCQKDLVGVVMNPS AP2C_MOUSE 202 74 -
NPLNLPCQKELVGAVMNPT AP2C_HUMAN 203 75 -