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PR01750

Identifier
AP2BTNSCPFCT  [View Relations]  [View Alignment]  
Accession
PR01750
No. of Motifs
4
Creation Date
22-MAY-2002
Title
Transcription factor AP-2 beta signature
Database References
PRINTS; PR01748 AP2TNSCPFCT
MIM; 601601; 169100
Literature References
1. WILLIAMS, T. AND TJIAN, R.
Analysis of the DNA-binding and activation properties of the human
transcription factor AP-2.
GENES DEV. 5 670-682 (1991).
 
2. WILLIAMS, T. AND TJIAN, R.
Characterization of a dimerization motif in AP-2 and its function in
heterologous DNA-binding proteins.
SCIENCE 251 1067-1071 (1991).
 
3. HILGER-EVERSHEIM, K., MOSER, M., SCHORLE, H. AND BUETTNER, R. 
Regulation roles of AP-2 transcription factors in vertebrate development, 
apoptosis and cell-cycle control, 
GENE 260 1-12 (2000).
 
4. JEAN, D., GERSHENWALD, J., HUANG, S., LUCA, M., HUDSON, M., TAINSKY, M.
AND BAR-ELI, M.
Loss of AP-2 results in up-regulation of MCAM/MUC18 and an increase in tumor
growth and metastasis of human melanoma cells.
J.BIOL.CHEM. 273 16501-16508 (1998).
 
5. ANTTILA, M., KELLOKOSKI, J., MOISIO, K., MITCHELL, P., SAARIKOSKI, S., 
SYRJANEN, K. AND KOSMA, V.
Expression of transcription factor AP-2alpha predicts survival in epithelial
ovarian cancer.
BR.J.CANCER 82 1974-1983 (2000).
 
6.MOSER, M., IMHOF, A., PSCHERER, A., BAUER, R., AMSELGRUBER, W., SINOWATZ,
F., HOFSTADTER, F., SCHULE, R AND BUETTNER, R.
Cloning and characterization of a second AP-2 transcription factor: AP-2 
beta.
DEVELOPMENT 121 2779-2788 (1995).
 
7. SATODA, M., ZHAO, F., DIAZ, G., BURN, J., GOODSHIP, J., DAVIDSON, H., 
PIERPONT, M. AND GELB B.
Mutations in TFAP2B cause Char syndrome, a familial form of patent ductus
arteriosus.
NAT.GENET. 25 42-46 (2000).

Documentation
Activator protein-2 (AP-2) transcription factors constitute a family of
closely related and evolutionarily conserved proteins that bind to the DNA 
consensus sequence GCCNNNGGC and stimulate target gene transcription [1,2].
Four different isoforms of AP-2 have been identifed in mammals, termed AP-2
alpha, beta, gamma and delta. Each family member shares a common structure, 
possessing a proline/glutamine-rich domain in the N-terminal region, which 
is responsible for transcriptional activation [1], and a helix-span-helix
domain in the C-terminal region, which mediates dimerisation and site-
specific DNA binding [2].
 
The AP-2 family have been shown to be critical regulators of gene expression
during embryogenesis. They regulate the development of facial prominence and
limb buds, and are essential for cranial closure and development of the lens
[3]; they have also been implicated in tumorigenesis. AP-2 protein 
expression levels have been found to affect cell transformation, tumour 
growth and metastasis, and may predict survival in some types of cancer [4,5].
 
AP-2 beta was originally isolated by cDNA screening of a human genomic 
library [6]. The protein was designated AP-2 beta on the basis of its high 
sequence similarity to AP-2 alpha, its site-specific DNA binding, and its
ability to stimulate transcription [6]. Defects in AP-2 beta have been shown
to cause Char syndrome, an autosomal dominant trait characterised by patent
ductus arteriosus, facial dysmorphism and hand anomalies [7].
 
AP2BTNSCPFCT is a 4-element fingerprint that provides a signature for
transcription factor AP-2 beta proteins. The fingerprint was derived from 
an initial alignment of 3 sequences: the motifs were drawn from conserved 
regions in the N-terminal half of the alignment, focusing on those sections 
that characterise AP-2 beta but distinguish it from other family members -
motif 1 resides within the transcriptional activation domain; and motifs 2-4
lie between the transcriptional activation domain and the dimerisation
domain. A single iteration on SPTR40_20f was required to reach convergence,
no further sequences being identified beyond the starting set. 
Summary Information
3 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
43333
30000
20000
1234
True Positives
AP2B_HUMAN    AP2B_MOUSE    O93346        
Sequence Titles
AP2B_HUMAN  Transcription factor AP-2 beta (AP2-beta) (Activating enhancer-binding protein 2 beta) - Homo sapiens (Human). 
AP2B_MOUSE Transcription factor AP-2 beta (AP2-beta) (Activating enhancer-binding protein 2 beta) - Mus musculus (Mouse).
O93346 TRANSCRIPTION FACTOR AP-2 BETA - Gallus gallus (Chicken).
Scan History
SPTR40_20f 1  250  NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
GVPSHSSRLSQLGSVSQGP AP2B_HUMAN 21 21 -
GVPSHSSRLSQLGSVSQGP AP2B_MOUSE 21 21 -
GVPSHSSRLSQLGSVSQGP O93346 21 21 -

Motif 2 width=13
Element Seqn Id St Int Rpt
DSLSLHGLGHPGM AP2B_HUMAN 153 113 -
DSLSLHGLGHPGM AP2B_MOUSE 153 113 -
DSLSLHGIGHPGM O93346 153 113 -

Motif 3 width=11
Element Seqn Id St Int Rpt
DANNSGMNLLD AP2B_HUMAN 173 7 -
DANNSGMNLLD AP2B_MOUSE 173 7 -
DANNSGMNLLD O93346 173 7 -

Motif 4 width=13
Element Seqn Id St Int Rpt
DGFLGGMSVNTGE AP2B_HUMAN 205 21 -
DGFLGGMSVNTGE AP2B_MOUSE 205 21 -
DGFLGGISVNTGE O93346 205 21 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
GVPSHSSRLSQLGSVSQGP AP2B_HUMAN 21 21 -
GVPSHSSRLSQLGSVSQGP AP2B_MOUSE 21 21 -
GVPSHSSRLSQLGSVSQGP O93346 21 21 -

Motif 2 width=13
Element Seqn Id St Int Rpt
DSLSLHGLGHPGM AP2B_HUMAN 153 113 -
DSLSLHGLGHPGM AP2B_MOUSE 153 113 -
DSLSLHGIGHPGM O93346 153 113 -

Motif 3 width=11
Element Seqn Id St Int Rpt
DANNSGMNLLD AP2B_HUMAN 173 7 -
DANNSGMNLLD AP2B_MOUSE 173 7 -
DANNSGMNLLD O93346 173 7 -

Motif 4 width=13
Element Seqn Id St Int Rpt
DGFLGGMSVNTGE AP2B_HUMAN 205 21 -
DGFLGGMSVNTGE AP2B_MOUSE 205 21 -
DGFLGGISVNTGE O93346 205 21 -