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PR01748

Identifier
AP2TNSCPFCT  [View Relations]  [View Alignment]  
Accession
PR01748
No. of Motifs
3
Creation Date
22-MAY-2002
Title
Transcription factor AP-2 signature
Database References
PRINTS; PR01749 AP2ATNSCPFCT; PR01750 AP2BTNSCPFCT; PR01751 AP2CTNSCPFCT

PFAM; PF03299 TF_AP-2
INTERPRO; IPR004979
Literature References
1. WILLIAMS, T. AND TJIAN, R.
Analysis of the DNA-binding and activation properties of the human
transcription factor AP-2.
GENES DEV. 5 670-682 (1991).
 
2. WILLIAMS, T. AND TJIAN, R.
Characterization of a dimerization motif in AP-2 and its function in
heterologous DNA-binding proteins.
SCIENCE 251 1067-1071 (1991).
 
3. HILGER-EVERSHEIM, K., MOSER, M., SCHORLE, H. AND BUETTNER, R. 
Regulation roles of AP-2 transcription factors in vertebrate development, 
apoptosis and cell-cycle control, 
GENE 260 1-12 (2000).
 
4. JEAN, D., GERSHENWALD, J., HUANG, S., LUCA, M., HUDSON, M., TAINSKY, M.
AND BAR-ELI, M.
Loss of AP-2 results in up-regulation of MCAM/MUC18 and an increase in tumor
growth and metastasis of human melanoma cells.
J.BIOL.CHEM. 273 16501-16508 (1998).
 
5. ANTTILA, M., KELLOKOSKI, J., MOISIO, K., MITCHELL, P., SAARIKOSKI, S., 
SYRJANEN, K. AND KOSMA, V.
Expression of transcription factor AP-2alpha predicts survival in epithelial
ovarian cancer.
BR.J.CANCER 82 1974-1983 (2000).

Documentation
Activator protein-2 (AP-2) transcription factors constitute a family of
closely related and evolutionarily conserved proteins that bind to the DNA 
consensus sequence GCCNNNGGC and stimulate target gene transcription [1,2].
Four different isoforms of AP-2 have been identifed in mammals, termed AP-2
alpha, beta, gamma and delta. Each family member shares a common structure, 
possessing a proline/glutamine-rich domain in the N-terminal region, which 
is responsible for transcriptional activation [1], and a helix-span-helix
domain in the C-terminal region, which mediates dimerisation and site-
specific DNA binding [2].
 
The AP-2 family have been shown to be critical regulators of gene expression
during embryogenesis. They regulate the development of facial prominence and
limb buds, and are essential for cranial closure and development of the lens
[3]; they have also been implicated in tumorigenesis. AP-2 protein 
expression levels have been found to affect cell transformation, tumour 
growth and metastasis, and may predict survival in some types of cancer [4,5].
 
AP2TNSCPFCT is a 3-element fingerprint that provides a signature for the
AP-2 transcription factor family. The fingerprint was derived from an 
initial alignment of 12 sequences: the motifs were drawn from conserved 
regions in the C-terminal half of the alignment, focusing on those sections
that characterise the AP-2 family - motifs 1 and 2 lie between the
transcriptional activation domain and the helix-span-helix dimerisation 
domain; and motif 3 lies within the dimerisation domain. Three iterations on
SPTR40_20f were required to reach convergence, at which point a true set 
comprising 20 sequences was identified.
Summary Information
20 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3202020
2000
123
True Positives
AP2A_HUMAN    AP2A_MOUSE    AP2A_RAT      AP2A_SHEEP    
AP2B_HUMAN AP2B_MOUSE AP2C_HUMAN AP2C_MOUSE
O13111 O76923 O93346 O96378
Q09585 Q8UVE5 Q8UVE6 Q91867
Q91ZK0 Q96SH0 Q96SH1 Q99L72
Sequence Titles
AP2A_HUMAN  Transcription factor AP-2 alpha (AP2-alpha) (Activating enhancer- binding protein 2 alpha) (AP-2 transcription factor) (Activator protein-2) (AP-2) - Homo sapiens (Human). 
AP2A_MOUSE Transcription factor AP-2 alpha (AP2-alpha) (Activating enhancer- binding protein 2 alpha) (Activator protein-2) (AP-2) - Mus musculus (Mouse).
AP2A_RAT Transcription factor AP-2 alpha (AP2-alpha) (Activating enhancer- binding protein 2 alpha) (Activator protein-2) (AP-2) - Rattus norvegicus (Rat).
AP2A_SHEEP Transcription factor AP-2 alpha (AP2-alpha) (Activating enhancer- binding protein 2 alpha) (Activator protein-2) (AP-2) - Ovis aries (Sheep).
AP2B_HUMAN Transcription factor AP-2 beta (AP2-beta) (Activating enhancer-binding protein 2 beta) - Homo sapiens (Human).
AP2B_MOUSE Transcription factor AP-2 beta (AP2-beta) (Activating enhancer-binding protein 2 beta) - Mus musculus (Mouse).
AP2C_HUMAN Transcription factor AP-2 gamma (AP2-gamma) (Activating enhancer- binding protein 2 gamma) (Transcription factor ERF-1) - Homo sapiens (Human).
AP2C_MOUSE Transcription factor AP-2 gamma (AP2-gamma) (Activating enhancer- binding protein 2 gamma) (AP-2.2) - Mus musculus (Mouse).
O13111 AP-2 TRANSCRIPTION FACTOR - Gallus gallus (Chicken).
O76923 AP-2 PROTEIN - Drosophila melanogaster (Fruit fly).
O93346 TRANSCRIPTION FACTOR AP-2 BETA - Gallus gallus (Chicken).
O96378 TRANSCRIPTION FACTOR AP-2 (RE17884P) - Drosophila melanogaster (Fruit fly).
Q09585 HYPOTHETICAL 43.1 KDA PROTEIN - Caenorhabditis elegans.
Q8UVE5 TRANSCRIPTION FACTOR AP2 ALPHA 2 - Brachydanio rerio (Zebrafish) (Zebra danio).
Q8UVE6 TRANSCRIPTION FACTOR AP2 ALPHA 1 - Brachydanio rerio (Zebrafish) (Zebra danio).
Q91867 TRANSCRIPTION FACTOR - Xenopus laevis (African clawed frog).
Q91ZK0 TRANSCRIPTION FACTOR AP-2DELTA - Mus musculus (Mouse).
Q96SH0 DJ290I10.2.2 (TRANSCRIPTION FACTOR AP-2 ALPHA (ACTIVATING ENHANCER-BINDING PROTEIN 2 ALPHA), ISOFORM 2) - Homo sapiens (Human).
Q96SH1 DJ290I10.2.1 (TRANSCRIPTION FACTOR AP-2 ALPHA (ACTIVATING ENHANCER-BINDING PROTEIN 2 ALPHA), ISOFORM 1) - Homo sapiens (Human).
Q99L72 TRANSCRIPTION FACTOR AP-2, GAMMA - Mus musculus (Mouse).
Scan History
SPTR40_20f 3  300  NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
LGGVLRRAKSKNGGR AP2A_HUMAN 249 249 -
LGGVLRRAKSKNGGR Q91867 238 238 -
LGGVLRRAKSKNGGR O13111 249 249 -
LGGVLRRAKSKNGGR AP2A_SHEEP 303 303 -
LGGVLRRAKSKNGGR AP2A_RAT 249 249 -
LGGVLRRAKSKNGGR AP2A_MOUSE 249 249 -
LGGVLRRAKSKNGGR AP2C_HUMAN 262 262 -
LGGVLRRAKSKNGGR AP2C_MOUSE 261 261 -
LGGVLRRAKSKNGGR AP2B_HUMAN 257 257 -
LGGVLRRAKSKNGGR O93346 257 257 -
LGGVLRRAKSKNGGR O93346 257 257 -
LGGVLRRAKSKNGGR AP2B_MOUSE 257 257 -

Motif 2 width=16
Element Seqn Id St Int Rpt
SLREKLDKIGLNLPAG AP2A_HUMAN 264 0 -
SLREKLDKIGLNLPAG Q91867 253 0 -
SLREKLDKIGLNLPAG O13111 264 0 -
SLREKLDKIGLNLPAG AP2A_SHEEP 318 0 -
SLREKLDKIGLNLPAG AP2A_RAT 264 0 -
SLREKLDKIGLNLPAG AP2A_MOUSE 264 0 -
SLREKLDKIGLNLPAG AP2C_HUMAN 277 0 -
SLREKLDKIGLNLPAG AP2C_MOUSE 276 0 -
SLRERLEKIGLNLPAG AP2B_HUMAN 272 0 -
SLRERLEKIGLNLPAG O93346 272 0 -
SLRERLEKIGLNLPAG O93346 272 0 -
SLRERLEKIGLNLPAG AP2B_MOUSE 272 0 -

Motif 3 width=15
Element Seqn Id St Int Rpt
RRKAANVTLLTSLVE AP2A_HUMAN 280 0 -
RRKAANVTLLTSLVE Q91867 269 0 -
RRKAANVTLLTSLVE O13111 280 0 -
RRKAANVTLLTSLVE AP2A_SHEEP 334 0 -
RRKAANVTLLTSLVE AP2A_RAT 280 0 -
RRKAANVTLLTSLVE AP2A_MOUSE 280 0 -
RRKAAHVTLLTSLVE AP2C_HUMAN 293 0 -
RRKAAHVTLLTSLVE AP2C_MOUSE 292 0 -
RRKAANVTLLTSLVE AP2B_HUMAN 288 0 -
RRKAANVTLLTSLVE O93346 288 0 -
RRKAANVTLLTSLVE O93346 288 0 -
RRKAANVTLLTSLVE AP2B_MOUSE 288 0 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
LGGVLRRAKSKNGGR AP2A_HUMAN 249 249 -
LGGVLRRAKSKNGGR Q96SH1 251 251 -
LGGVLRRAKSKNGGR Q96SH0 251 251 -
LGGVLRRAKSKNGGR Q91867 238 238 -
LGGVLRRAKSKNGGR Q8UVE6 243 243 -
LGGVLRRAKSKNGGR Q8UVE5 249 249 -
LGGVLRRAKSKNGGR O13111 249 249 -
LGGVLRRAKSKNGGR AP2A_SHEEP 303 303 -
LGGVLRRAKSKNGGR AP2A_RAT 249 249 -
LGGVLRRAKSKNGGR AP2A_MOUSE 249 249 -
LGGVLRRAKSKNGGR AP2C_HUMAN 262 262 -
LGGVLRRAKSKNGGR Q99L72 261 261 -
LGGVLRRAKSKNGGR AP2C_MOUSE 261 261 -
LGGVLRRAKSKNGGR AP2B_HUMAN 257 257 -
LGGVLRRAKSKNGGR O93346 257 257 -
LGGVLRRAKSKNGGR O93346 257 257 -
LGGVLRRAKSKNGGR AP2B_MOUSE 257 257 -
LGGVLRRAKSKNGGR O76923 275 275 -
LGGVLRRAKSKNGGR O96378 279 279 -
LGGILRRAKSKNGGR Q91ZK0 249 249 -
LGGILRKAKSKDGGK Q09585 208 208 -

Motif 2 width=16
Element Seqn Id St Int Rpt
SLREKLDKIGLNLPAG AP2A_HUMAN 264 0 -
SLREKLDKIGLNLPAG Q96SH1 266 0 -
SLREKLDKIGLNLPAG Q96SH0 266 0 -
SLREKLDKIGLNLPAG Q91867 253 0 -
SLREKLDKIGLNLPAG Q8UVE6 258 0 -
SLREKLDKIGLNLPAG Q8UVE5 264 0 -
SLREKLDKIGLNLPAG O13111 264 0 -
SLREKLDKIGLNLPAG AP2A_SHEEP 318 0 -
SLREKLDKIGLNLPAG AP2A_RAT 264 0 -
SLREKLDKIGLNLPAG AP2A_MOUSE 264 0 -
SLREKLDKIGLNLPAG AP2C_HUMAN 277 0 -
SLREKLDKIGLNLPAG Q99L72 276 0 -
SLREKLDKIGLNLPAG AP2C_MOUSE 276 0 -
SLRERLEKIGLNLPAG AP2B_HUMAN 272 0 -
SLRERLEKIGLNLPAG O93346 272 0 -
SLRERLEKIGLNLPAG O93346 272 0 -
SLRERLEKIGLNLPAG AP2B_MOUSE 272 0 -
LLREKLEKIGLNLPAG O76923 290 0 -
LLREKLEKIGLNLPAG O96378 294 0 -
CLREKLDRLGLNLPAG Q91ZK0 264 0 -
TLRDSLKKLGLTLPAG Q09585 223 0 -

Motif 3 width=15
Element Seqn Id St Int Rpt
RRKAANVTLLTSLVE AP2A_HUMAN 280 0 -
RRKAANVTLLTSLVE Q96SH1 282 0 -
RRKAANVTLLTSLVE Q96SH0 282 0 -
RRKAANVTLLTSLVE Q91867 269 0 -
RRKAANVTLLTSLVE Q8UVE6 274 0 -
RRKAANVTLLTSLVE Q8UVE5 280 0 -
RRKAANVTLLTSLVE O13111 280 0 -
RRKAANVTLLTSLVE AP2A_SHEEP 334 0 -
RRKAANVTLLTSLVE AP2A_RAT 280 0 -
RRKAANVTLLTSLVE AP2A_MOUSE 280 0 -
RRKAAHVTLLTSLVE AP2C_HUMAN 293 0 -
RRKAAHVTLLTSLVE Q99L72 292 0 -
RRKAAHVTLLTSLVE AP2C_MOUSE 292 0 -
RRKAANVTLLTSLVE AP2B_HUMAN 288 0 -
RRKAANVTLLTSLVE O93346 288 0 -
RRKAANVTLLTSLVE O93346 288 0 -
RRKAANVTLLTSLVE AP2B_MOUSE 288 0 -
RRKAANVTLLTSLVE O76923 306 0 -
RRKAANVTLLTSLVE O96378 310 0 -
RRKAANVTLLTSLVE Q91ZK0 280 0 -
RRKQANVTAWTALVE Q09585 239 0 -