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PR01742

Identifier
SEPTIN7  [View Relations]  [View Alignment]  
Accession
PR01742
No. of Motifs
4
Creation Date
28-JUN-2002
Title
Septin 7 signature
Database References
Literature References
1. HARTWELL, L.
Genetic control of the cell division cycle in yeast. IV. Genes controlling 
bud emergence and cytokinesis.
EXP.CELL RES. 69 265-276 (1971).
 
2. HAARER, B. AND PRINGLE, J.
Immunofluorescence localization of the Saccharomyces cerevisiae CDC12 gene 
product to the vicinity of the 10-nm filaments in the mother-bud neck.
MOL.CELL BIOL. 7 3678-3687 (1987).
 
3. LONGTINE, M., THEESFELD, C., MCMILLAN, J., WEAVER, E., PRINGLE, J.
AND LEW, D.
Septin-dependent assembly of a cell cycle-regulatory module in 
Saccharomyces cerevisiae. 
MOL.CELL BIOL. 20 4049-4061 (2000).
 
4. FIELD, C. AND KELLOG, D.
Septins: cytoskeletal polymers or signalling GTPases? 
TRENDS CELL BIOL. 9 387-394 (1999).
 
5. BEITES, C., XIE, H., BOWSER, R. AND TRIMBLE, W.
The septin CDCrel-1 binds syntaxin and inhibits exocytosis.
NAT.NEUROSCI. 2 434-439. (1999).
 
6. KINOSHITA, M. AND NODA, M.
Roles of septins in the mammalian cytokinesis machinery.
CELL STRUCT.FUNCT. 26 667-670 (2001).
 
7. NAKATSURU, S., SUDO, K. AND NAKAMURA, Y.
Molecular Cloning of a Novel Human cDNA Homologous to CDC10 in Saccharomyces
cerevisiae.
BIOCHEM.BIOPHYS.RES.COMMUN. 202 82-87 (1994).

Documentation
Septins constitute a eukaryotic family of guanine nucleotide-binding proteins.
Members of the family were first identified by genetic screening for 
Saccharomyces cerevisiae mutants defective in cytokinesis [1]. Temperature-
sensitive mutations in four genes, CDC3, CDC10, CDC11 and CDC12, were found
to cause cell-cycle arrest and defects in bud growth and cytokinesis. The
protein products of these genes localise at the division plane between
mother and daughter cells, indicating a role in mother-daughter separation
during cytokinesis [2]. Members of the family were therefore termed septins
to reflect their role in septation and cell division. The identification of
septin homologues in higher eukaryotes, which localise to the cleavage
furrow in dividing cells, supports an orthologous function in cytokinesis.
Septins have since been identified in most eukaryotes, except plants [3].
 
Septins are approximately 40-50kDa in molecular mass, and typically comprise
a conserved central core domain (>35% sequence identity between mammalian
and yeast homologues) flanked by more divergent N- and C-termini. Most
septins possess a P-loop motif in their N-terminal domain (which is
characteristic of GTP-binding proteins), and a predicted C-terminal coiled-
coil domain [4].
 
A number of septin interaction partners have been identified in yeast, 
many of which are components of the budding site selection machinery, kinase
cascades or of the ubiquitination pathway [3,4]. It has therefore been 
proposed that septins may act as a scaffold that provides an interaction 
matrix for other proteins. In mammals, septins have been shown to interact
regulate vesicle dynamics [5]. Mammalian septins have also been implicated
in a variety of other cellular processes, including apoptosis, carcinogenesis
and neurodegeneration [6].
 
Cloning of a novel human cDNA, whose protein product shared a high degree of
sequence similarity with Saccharomyces cerevisiae CDC10, was reported in
1994 [7]. This protein was termed hCDC10 and is also known as septin 7.
Orthologues have since been identified in rat and mouse.      
 
SEPTIN7 is a 4-element fingerprint that provides a signature for septin 7 
(CDC10 homologue) proteins. The fingerprint was derived from an initial 
alignment of 3 sequences: the motifs were drawn from conserved regions 
spanning the C-terminal half of the alignment, focusing on those sections
that characterise septin 7 but distinguish it from other family members - 
motif 1 resides within the central core domain; and motifs 2-4 lie within 
the C-terminal putative coiled-coil domain. A single iteration on SPTR40_20f
was required to reach convergence, no further sequences being identified 
beyond the starting set. 
Summary Information
3 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
43333
30000
20000
1234
True Positives
SEP7_HUMAN    SEP7_MOUSE    SEP7_RAT      
Sequence Titles
SEP7_HUMAN  Septin 7 (CDC10 protein homolog) - Homo sapiens (Human). 
SEP7_MOUSE Septin 7 (CDC10 protein homolog) - Mus musculus (Mouse).
SEP7_RAT Septin 7 (CDC10 protein homolog) - Rattus norvegicus (Rat).
Scan History
SPTR40_20f 1  300  NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
ENKLVKKIKDRLP SEP7_HUMAN 214 214 -
ENKLVKKIKDRLP SEP7_RAT 232 232 -
ENKLVKKIKDRLP SEP7_MOUSE 232 232 -

Motif 2 width=13
Element Seqn Id St Int Rpt
KGQLTKSPLAQME SEP7_HUMAN 309 82 -
KGQLTKSPLAQME SEP7_RAT 327 82 -
KGQLTKSPLAQME SEP7_MOUSE 327 82 -

Motif 3 width=12
Element Seqn Id St Int Rpt
HEQMKKNLEAQH SEP7_HUMAN 364 42 -
HEQMKKNLEAQH SEP7_RAT 382 42 -
HEQMKKNLEAQH SEP7_MOUSE 382 42 -

Motif 4 width=15
Element Seqn Id St Int Rpt
SSRTLEKNKKKGKIF SEP7_HUMAN 404 28 -
SSRTLEKNKKKGKIF SEP7_RAT 422 28 -
SSRTLEKNKKKGKIF SEP7_MOUSE 422 28 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
ENKLVKKIKDRLP SEP7_HUMAN 214 214 -
ENKLVKKIKDRLP SEP7_RAT 232 232 -
ENKLVKKIKDRLP SEP7_MOUSE 232 232 -

Motif 2 width=13
Element Seqn Id St Int Rpt
KGQLTKSPLAQME SEP7_HUMAN 309 82 -
KGQLTKSPLAQME SEP7_RAT 327 82 -
KGQLTKSPLAQME SEP7_MOUSE 327 82 -

Motif 3 width=12
Element Seqn Id St Int Rpt
HEQMKKNLEAQH SEP7_HUMAN 364 42 -
HEQMKKNLEAQH SEP7_RAT 382 42 -
HEQMKKNLEAQH SEP7_MOUSE 382 42 -

Motif 4 width=15
Element Seqn Id St Int Rpt
SSRTLEKNKKKGKIF SEP7_HUMAN 404 28 -
SSRTLEKNKKKGKIF SEP7_RAT 422 28 -
SSRTLEKNKKKGKIF SEP7_MOUSE 422 28 -