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PR01730

Identifier
INFPOTNTIATR  [View Relations]  [View Alignment]  
Accession
PR01730
No. of Motifs
4
Creation Date
24-JUN-2002
Title
Macrophage infectivity potentiator signature
Database References

PDB; 1FD9
SCOP; 1FD9
CATH; 1FD9
Literature References
1. HACKER, J., OTT, M., WINDERMEYER, E., LUDWIG, B. AND FISCHER, G.
Analysis of virulence factors of Legionella pneumophila. 
ZENTRALBL.BAKTERIOL. 278 348-358 (1993).
 
2. ENGLEBERG, N.C., CARTER, C., WEBER, D.R., CIANCIOTTO, N.P. AND 
EISENSTEIN, B.I.
DNA sequence of mip, a Legionella pneumophila gene associated with 
macrophage infectivity.
INFECT.IMMUN. 57 1263-1270 (1989).
 
3. FISCHER, G., BANG, H., LUDWIG, B., MANN, K. AND HACKER, J.
Mip protein of Legionella pneumophila exhibits peptidyl-prolyl-cis/trans
isomerase (PPIase) activity. 
MOL.MICROBIOL. 6 1375-1383 (1992).
 
4. LUDWIG, B., RAHFELD, J., SCHMIDT, B., MANN, K., WINDERMEYER, E., FISCHER,
G. AND HACKER J.
Characterization of mip proteins of Legionella pneumophila. 
FEMS MICROBIOL.LETT. 118 23-30 (1994).
 
5. RIBOLDI-TUNNICLIFFE, A., KONIG, B., JESSEN, S., WEISS, M.S., RAHFELD, J.,
HACKER, J., FISCHER, G. AND HILGENFELD, R.
Crystal structure of Mip, a prolylisomerase from Legionella pneumophila.
NAT.STRUCT.BIOL. 8 779-783 (2001).

Documentation
Legionella pneumophila, the causative agent of Legionnaire's disease, is a
facultative intracellular microbe that commonly infects human lung 
monocytes and macrophages and causes pneumonia [1]. It is water-borne and 
highly virulent, relying on several specific pathogenic factors to invade 
and infect the alveolar tissue. However, once grown to stationary phase in 
culture, the pathogen spontaneously converts to an avirulent state [1]. 
 
The major virulence factor expressed by Legionella pneumophila is the 
macrophage infectivity potentiator (Mip) [2]. Site-directed mutagenesis
studies of this protein in vitro severely impaired the intracellular
infection of human macrophages by L.pneumophila, causing it to lose
its potent antigenic activity [2]. Further studies into the enzymatic 
activity of Mip have revealed that it plays a similar role to eukaryotic
FK506-binding proteins. In vivo, it acts as a peptidyl-prolyl-cis/trans-
isomerase (PPIase) on oligopeptides [3], although it is unclear whether this
forms part of the virulence process. Substitution of Asp142 of the mature 
protein by Leu severely reduces the PPIase activity of Mip [4].
 
The structure of Mip has been resolved to 2.41A by X-ray crystallography [5],
revealing the virulence factor to exist as a homodimer. Each monomer 
consists of an N-terminal dimerisation module, a long central connecting
alpha-helix and a conserved PPIase domain at the C-terminus [5]. 
 
INFPOTNTIATR is a 4-element fingerprint that provides a signature for the 
macrophage infectivity potentiator proteins. The fingerprint was derived
from an initial alignment of 6 sequences: the motifs were drawn from
conserved regions spanning the N-terminal portion of the alignment (~80
amino acids) - motif 1 encodes the N-terminus of alpha-helix 1; motif 2 
spans the C-terminus of helix 1 and the N-terminus of helix 2; motif 3
encompasses the N-terminus of the long connecting alpha-helix 3; and motif
4 encodes the C-terminus of the same helix. Two iterations on SPTR40_20f 
were required to reach convergence, at which point a true set comprising 41
sequences was identified.
Summary Information
41 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
441414141
30000
20000
1234
True Positives
MIP_LEGLO     MIP_LEGMI     MIP_LEGPN     O32746        
O32747 O32749 O32750 O32751
O32752 O32753 O32759 O32760
O32761 O32762 O32763 O32764
O32766 O32767 O32768 O32820
O32825 O32826 O32827 O32828
O32834 O32835 O32836 O32837
O32838 O32839 O32840 O32841
O32842 O32843 O32844 O32845
Q53327 Q933L8 Q9LAU8 Q9LAU9
Q9X4F3
Sequence Titles
MIP_LEGLO   Outer membrane protein MIP precursor (Macrophage infectivity potentiator) (Peptidyl-prolyl cis-trans isomerase) (EC 5.2.1.8) (PPiase) (Rotamase) - Legionella longbeachae. 
MIP_LEGMI Outer membrane protein MIP precursor (Macrophage infectivity potentiator) (Peptidyl-prolyl cis-trans isomerase) (EC 5.2.1.8) (PPiase) (Rotamase) - Legionella micdadei.
MIP_LEGPN Outer membrane protein MIP precursor (Macrophage infectivity potentiator) (Peptidyl-prolyl cis-trans isomerase) (EC 5.2.1.8) (PPiase) (Rotamase) - Legionella pneumophila.
O32746 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella adelaidensis.
O32747 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella anisa.
O32749 MACROPHAGE INFECTIVITY POTENTIATOR (MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN) - Legionella birminghamensis.
O32750 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella bozemanii.
O32751 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella brunensis.
O32752 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella cherrii.
O32753 MACROPHAGE INFECTIVITY POTENTIATOR (MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN) - Legionella cincinnatiensis.
O32759 MACROPHAGE INFECTIVITY POTENTIATOR (MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN) - Fluoribacter dumoffii.
O32760 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella erythra.
O32761 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella fairfieldensis.
O32762 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella feeleii.
O32763 MACROPHAGE INFECTIVITY POTENTIATOR - Fluoribacter gormanii.
O32764 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella gratiana.
O32766 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella hackeliae.
O32767 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella israelensis.
O32768 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella jordanis.
O32820 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella lansingensis.
O32825 MACROPHAGE INFECTIVITY POTENTIATOR (MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN) - Tatlockia maceachernii.
O32826 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella moravica.
O32827 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella nautarum.
O32828 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella oakridgensis.
O32834 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella parisiensis.
O32835 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella quateirensis.
O32836 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella quinlivanii.
O32837 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella rubrilucens.
O32838 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella sainthelensi.
O32839 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella santicrucis.
O32840 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella shakespearei.
O32841 MACROPHAGE INFECTIVITY POTENTIATOR (MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN) - Legionella spiritensis.
O32842 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella steigerwaltii.
O32843 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella tucsonensis.
O32844 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella wadsworthii.
O32845 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella worsleiensis.
Q53327 MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN (MIP) - Legionella micdadei.
Q933L8 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella pneumophila.
Q9LAU8 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella pneumophila.
Q9LAU9 MACROPHAGE INFECTIVITY POTENTIATOR - Legionella pneumophila.
Q9X4F3 MACROPHAGE INFECTIVITY POTENTIATOR - Amoeba proteus symbiotic bacterium.
Scan History
SPTR40_20f 2  150  NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
TSLTTDKDKLSYSIG MIP_LEGLO 25 25 -
TSLATDKDKLSYSIG MIP_LEGPN 25 25 -
TSLATDKDKLSYSIG Q933L8 25 25 -
TSLPTDKDKLSYSIG O32840 25 25 -
TSLNSDMDKLSYSIG O32751 40 40 -
TSLTTDTEKLSYSIG MIP_LEGMI 34 34 -

Motif 2 width=13
Element Seqn Id St Int Rpt
KNFKNQGIDINPD MIP_LEGLO 44 4 -
KNFKNQGIDVNPE MIP_LEGPN 44 4 -
KNFKNQGIDVNPE Q933L8 44 4 -
KNFKTQGIDINPE O32840 44 4 -
KNFKKQGIDINPS O32751 59 4 -
KNFKKQGIEISPA MIP_LEGMI 53 4 -

Motif 3 width=15
Element Seqn Id St Int Rpt
TEEQMKDVLSKFQKD MIP_LEGLO 74 17 -
TEQQMKDVLNKFQKD MIP_LEGPN 74 17 -
TEQQMKDVLNKFQKD Q933L8 74 17 -
TEQQMKDVLNKFQKD O32840 74 17 -
TEQQMKDVLNKFQKD O32751 89 17 -
TDDQMKDVLNKFQKD MIP_LEGMI 83 17 -

Motif 4 width=20
Element Seqn Id St Int Rpt
KRSAEFNKKAEENKAKGDAF MIP_LEGLO 92 3 -
KRTAEFNKKADENKVKGEAF MIP_LEGPN 92 3 -
KRTAEFNKKADENKVKGEAF Q933L8 92 3 -
KRTSEFNKKADENKSKGEAF O32840 92 3 -
KRTAEFSKKADENKAKGEAF O32751 107 3 -
KRSAEFNKKAEENKSKGEAF MIP_LEGMI 101 3 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
TSLVTDKDKLSYSIG O32763 25 25 -
TSLVTDKDKLSYSIG O32759 25 25 -
TSLVTDKDKLSYSIG O32752 25 25 -
TSLVTDKDKLSYSIG O32842 25 25 -
TSLVTDKDKLSYSIG O32750 25 25 -
TSLTTDKDKLSYSIG O32753 25 25 -
TSLTTDKDKLSYSIG O32838 25 25 -
TSLVTDKDKLSYSIG O32747 24 24 -
TSLVTDKDKLSYSIG O32834 25 25 -
TSLVTDKDKLSYSIG O32843 25 25 -
TSLTTDKDKLSYSIG O32839 25 25 -
TSLTTDKDKLSYSIG MIP_LEGLO 25 25 -
TSLATDKDKLSYSIG MIP_LEGPN 25 25 -
TSLATDKDKLSYSIG Q933L8 25 25 -
TSLPTDKDKLSYSIG O32826 24 24 -
TSLPTDKDKLSYSIG O32835 24 24 -
TSLVTDKDKLSYSIG O32844 25 25 -
TSLPTDKDKLSYSIG O32840 25 25 -
TSLATDKDKLSYSIG Q9LAU8 25 25 -
TSLATDKDKLSYSIG Q9LAU9 25 25 -
TSLTTDKDKLSYSIG O32845 24 24 -
TSLNSDMDKLSYSIG O32768 42 42 -
TSLNSDMDKLSYSIG O32751 40 40 -
TSLATDKDKLSYSIG O32764 25 25 -
TSLNSDMDKLSYSIG O32762 28 28 -
TSLNSDMDKLSYSIG O32761 32 32 -
TSLNSDMDKLSYSIG O32766 40 40 -
TSLNSDTDKLSYSIG O32820 40 40 -
TSLTTDTEKLSYSIG MIP_LEGMI 34 34 -
TSLTTDTEKLSYSIG Q53327 34 34 -
ASLSTDTDKLSYSIG O32827 32 32 -
TSLTTDMDKLSYSIG Q9X4F3 35 35 -
TSLATDTEKLSYSIG O32825 35 35 -
ATLTTDIDKLSYSIG O32760 39 39 -
ATLNTDIDKLSYSIG O32837 39 39 -
TNLNTDTDKLSYSIG O32841 26 26 -
TQLTTEMDKLSYSIG O32746 25 25 -
VTLSTDMDKLSYSIG O32836 24 24 -
VTLSTDMDKLSYSIG O32749 24 24 -
SSLTTDTDKLSYSIG O32828 25 25 -
SSLNTDVEKLSYSIG O32767 25 25 -

Motif 2 width=13
Element Seqn Id St Int Rpt
KNFKNQGIDINPE O32763 44 4 -
KNFKNQGIDINPE O32759 44 4 -
KNFKNQGIDINPD O32752 44 4 -
KNFKNQGIDINPD O32842 44 4 -
KNFKNQGIDINPD O32750 44 4 -
KNFKNQGIDINPE O32753 44 4 -
KNFKNQGIDINPD O32838 44 4 -
KNFKNQGIDINPD O32747 43 4 -
KNFKNQGIDINPD O32834 44 4 -
KNFKNQGIDINPD O32843 44 4 -
KNFKNQGIDINPE O32839 44 4 -
KNFKNQGIDINPD MIP_LEGLO 44 4 -
KNFKNQGIDVNPE MIP_LEGPN 44 4 -
KNFKNQGIDVNPE Q933L8 44 4 -
KNFKNQGIDVNPE O32826 43 4 -
KNFKNQGIDVNPE O32835 43 4 -
KNFKTQGIDINPD O32844 44 4 -
KNFKTQGIDINPE O32840 44 4 -
KNFKNQGIDVSPE Q9LAU8 44 4 -
KNFKNQGIDVSPE Q9LAU9 44 4 -
KNFKNQGIDVNPE O32845 43 4 -
KNFKKQGIDINPA O32768 61 4 -
KNFKKQGIDINPS O32751 59 4 -
KNFKNQGIEVNPE O32764 44 4 -
KNFKKQGIEISPA O32762 47 4 -
KNFKKQGIDISPA O32761 51 4 -
KNFKKQGIDISPA O32766 59 4 -
KNFKKQGIDINPS O32820 59 4 -
KNFKKQGIEISPA MIP_LEGMI 53 4 -
KNFKKQGIEISPA Q53327 53 4 -
KNFKKQGIDISPA O32827 51 4 -
KNLKKQGIDISPA Q9X4F3 54 4 -
KNFKKQGIEISPA O32825 54 4 -
KNFKKQGIEISPS O32760 58 4 -
KNFKKQGIEISPS O32837 58 4 -
KNFKKQGIDISPA O32841 45 4 -
KNFKRQGIDINPD O32746 44 4 -
KNFKKQGIEVNPA O32836 43 4 -
KNFKKQGIEINPT O32749 43 4 -
KNFKRQGIEINPQ O32828 44 4 -
KNFKKQGIEIDPQ O32767 44 4 -

Motif 3 width=15
Element Seqn Id St Int Rpt
TEQQMKDVLNKFQKD O32763 74 17 -
TEQQMKDVLNKFQKD O32759 74 17 -
TEQQMKDVLNKFQKD O32752 74 17 -
TEQQMKDVLNKFQKD O32842 74 17 -
TEQQMKDVLNKFQKE O32750 74 17 -
TEEQMKDVLSKFQKD O32753 74 17 -
TEEQMKDVLSKFQKD O32838 74 17 -
TEQQMKDVLNKFQKE O32747 73 17 -
TEQQMKDVLNKFQKE O32834 74 17 -
TEQQMKDVLNKFQKE O32843 74 17 -
TEEQMKDVLSKFQKD O32839 74 17 -
TEEQMKDVLSKFQKD MIP_LEGLO 74 17 -
TEQQMKDVLNKFQKD MIP_LEGPN 74 17 -
TEQQMKDVLNKFQKD Q933L8 74 17 -
TEQQMKDVLNKFQKD O32826 73 17 -
TEQQMKDVLNKFQKD O32835 73 17 -
TEQQMKDVLNKFQKD O32844 74 17 -
TEQQMKDVLNKFQKD O32840 74 17 -
TEQQMKDVLNKFQKD Q9LAU8 74 17 -
TEQQMKDVLNKFQKD Q9LAU9 74 17 -
TEQQMKDVLNKFQKD O32845 73 17 -
TEQQMKDVLNKFQKD O32768 91 17 -
TEQQMKDVLNKFQKD O32751 89 17 -
TEEQMKDVLSKFQKD O32764 74 17 -
TEQQMKDVLNKFQKD O32762 77 17 -
TEDQMKDVLNKFQKD O32761 81 17 -
TEQQMKDVLNKFQKD O32766 89 17 -
TEQQMKEVLNKFQKD O32820 89 17 -
TDDQMKDVLNKFQKD MIP_LEGMI 83 17 -
TDDQMKDVLNKFQKD Q53327 83 17 -
TEEQMKEVLNKFQKE O32827 81 17 -
TEQQMKDVLNKFQKD Q9X4F3 84 17 -
TDEQMKEVLNKFQKD O32825 84 17 -
TEDQMKDVLSKFQKD O32760 88 17 -
TEDQMKDVLSKFQKD O32837 88 17 -
TDQQMKDVLNKFQKD O32841 75 17 -
TDDQMKEVLNKFQKD O32746 74 17 -
TDDQMKEVLNKFQKD O32836 73 17 -
TEDQMKEVLNNFQKD O32749 73 17 -
TEEQMKDVLTKFQRD O32828 74 17 -
TEQQMRDVLNKFQQE O32767 74 17 -

Motif 4 width=20
Element Seqn Id St Int Rpt
KRSAEFNKKAEENKSKGEAF O32763 92 3 -
KRSAEFNKKAEENKAKGEAF O32759 92 3 -
KRSAEFNKKAEENKAKGEAF O32752 92 3 -
KRSAEFNKKAEENKAKGEAF O32842 92 3 -
KRSAEFNKKAEENKSKGEAF O32750 92 3 -
KRSAEFNKKAEENKAKGEAF O32753 92 3 -
KRSAEFNKKAEENKAKGEAF O32838 92 3 -
KRSAEFNKKAEENKSKGDAF O32747 91 3 -
KRSAEFNKKAEENKSKGDAF O32834 92 3 -
KRSAEFNKKAEENKSKGETF O32843 92 3 -
KRSAEFNKKAEENKAKGDAF O32839 92 3 -
KRSAEFNKKAEENKAKGDAF MIP_LEGLO 92 3 -
KRTAEFNKKADENKVKGEAF MIP_LEGPN 92 3 -
KRTAEFNKKADENKVKGEAF Q933L8 92 3 -
KRTSEFNKKAEENKSKGEAF O32826 91 3 -
KRTSEFNKKAEENKSKGEAF O32835 91 3 -
KRSAEFNKKAEENKSKGDSF O32844 92 3 -
KRTSEFNKKADENKSKGEAF O32840 92 3 -
KRTAEFNKKADENKVKGEAF Q9LAU8 92 3 -
KRTAEFNKKADENKVKGEAF Q9LAU9 92 3 -
KRTSEFNKKADENKLKGEAF O32845 91 3 -
KRNAEFSKKADENKSKGEAF O32768 109 3 -
KRTAEFSKKADENKAKGEAF O32751 107 3 -
KRSAEFNKKAEENKAKGDAF O32764 92 3 -
KRNAEFTKKAEENKAKGEAF O32762 95 3 -
KRTAEFNKKSEENKTKGEAF O32761 99 3 -
KRNADFNKKAEENKAKGDAF O32766 107 3 -
KRNAEFTKKAEENKIKGEAF O32820 107 3 -
KRSAEFNKKAEENKSKGEAF MIP_LEGMI 101 3 -
KRSAEFNKKAEENKSKGEAF Q53327 101 3 -
KRSAEFNKKSEENKSKGEAF O32827 99 3 -
RRTADFNKKAEENKTKGDSF Q9X4F3 102 3 -
KRSAEFNKKAEDNKAKGEAF O32825 102 3 -
KRNAEFTKKAEENKSKGEAF O32760 106 3 -
KRNAEFTKKAEENKSKGEAF O32837 106 3 -
RRSAEFAKKSEENKARGETF O32841 93 3 -
KRTAEFNKKAEQNKTKGESF O32746 92 3 -
KRNAEFSKKSEENKSKGEAF O32836 91 3 -
KRNAEFSKKSEENKAKGETF O32749 91 3 -
KRTAEYNKKAEDNKKKGEEF O32828 92 3 -
KRNAEFNKKAAENKAKGEAF O32767 92 3 -