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PR01716

Identifier
DEATHASSOCP3  [View Relations]  [View Alignment]  
Accession
PR01716
No. of Motifs
9
Creation Date
12-JUN-2002
Title
Death associated protein 3 family signature
Database References
Literature References
1. KISSIL, J.L., DEISS, L.P., BAYEWITC,H M., RAVEH, T., KHASPEKOV, G. AND
KIMCHI, A.
Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death.
J.BIOL.CHEM. 270 27932-27936 (1995).
 
2. BERGER, T., BRIGL, M., HERRMANN, J.M., VIELHAUER, V., LUCKOW, B.,
SCHLONDORFF, D. AND KRETZLER, M.
The apoptosis mediator mDAP-3 is a novel member of a conserved family of
mitochondrial proteins.
J.CELL SCI. 113 3603-3612 (2000).
 
3. KISSIL, J.L., COHEN, O., RAVEH, T. AND KIMCHI, A.
Structure-function analysis of an evolutionary conserved protein, DAP3,
which mediates TNF-alpha- and Fas-induced cell death.
EMBO J. 18 353-362 (1999).

Documentation
The DAP3 (death associated protein 3) family appear to be positive mediators
of apoptosis. Human DAP3 mediates inteferon-gamma-induced cell death [1], 
and also mediates the effects of Fas and TNF-alpha. The proteins are 
ubiquitously expressed and are localised to the mitochondrial matrix [1,2].
 
DAP3 contains a potential P-loop motif, suggesting that it might be an
ATP/GTP-binding protein. Mutations in the P-loop region of human DAP3 do 
not affect the overall stability of the protein, but do however reduce its
apoptopic effects by >2-fold [3]. By contrast, mutations in the murine DAP3
P-loop motif do abolish the pro-apoptopic effects [2]. 
 
DEATHASSOCP3 is a 9-element fingerprint that provides a signature for the
death associated protein 3 (DAP3) family. The fingerprint was derived from
an initial alignment of 5 sequences: the motifs were drawn from conserved
regions spanning the full alignment length - motif 3 spans the putative
nucleotide-binding domain. Two iterations on SPTR40_20f were required
to reach convergence, at which point a true set comprising 7 sequences
was identified.
Summary Information
7 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9777777777
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
DAP3_HUMAN    Q13044        Q8VCK8        Q9D5V9        
Q9ER88 Q9TVM5 Q9W253
Sequence Titles
DAP3_HUMAN  Mitochondrial 28S ribosomal protein S29 (MRP-S29) (Death-associated protein 3) (DAP-3) - Homo sapiens (Human). 
Q13044 IONIZING RADIATION RESISTANCE CONFERRING PROTEIN - Homo sapiens (Human).
Q8VCK8 DEATH ASSOCIATED PROTEIN 3 - Mus musculus (Mouse).
Q9D5V9 4921514D13RIK PROTEIN - Mus musculus (Mouse).
Q9ER88 DEATH ASSOCIATED PROTEIN 3 - Mus musculus (Mouse).
Q9TVM5 DAP-3 PROTEIN - Caenorhabditis elegans.
Q9W253 CG3633 PROTEIN (LD41023P) - Drosophila melanogaster (Fruit fly).
Scan History
SPTR40_20f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
PPRYMMQVKTFGE Q8VCK8 82 82 -
PPRYMMQVKTFGE Q9D5V9 82 82 -
PPRFVMQVKTFSE DAP3_HUMAN 84 84 -
PKQYEQQIKTFTE Q9W253 78 78 -
PAPLSKQMDTLGE Q9TVM5 54 54 -

Motif 2 width=12
Element Seqn Id St Int Rpt
RKPALELLGYLK Q8VCK8 100 5 -
RKPALELLGYLK Q9D5V9 100 5 -
RKPALELLHYLK DAP3_HUMAN 102 5 -
RNPALELLQYIK Q9W253 96 5 -
REPMAEVASCMR Q9TVM5 72 5 -

Motif 3 width=18
Element Seqn Id St Int Rpt
PAVRYLLYGEKGTGKTLS Q8VCK8 118 6 -
PAVRYLLYGEKGTGKTLS Q9D5V9 118 6 -
PAIRYLLYGEKGTGKTLS DAP3_HUMAN 120 6 -
PVVRYVLYGENGTGKTLT Q9W253 114 6 -
PALRLVLWGAFGTGKSVT Q9TVM5 90 6 -

Motif 4 width=12
Element Seqn Id St Int Rpt
HFCARHDWLILH Q8VCK8 141 5 -
HFCARHDWLILH Q9D5V9 141 5 -
HFCAKQDWLILH DAP3_HUMAN 143 5 -
HYGALNDFLLVH Q9W253 137 5 -
HHAYNNKWAIVH Q9TVM5 113 5 -

Motif 5 width=15
Element Seqn Id St Int Rpt
PLEASTWLKNFKTTN Q8VCK8 179 26 -
PLEGSTWLKNFKTTN Q9D5V9 179 26 -
PLEASTWLKNFKTTN DAP3_HUMAN 181 26 -
PFDAAAWLVHFKTQN Q9W253 175 26 -
PSNSVSILQNFKQQN Q9TVM5 151 26 -

Motif 6 width=12
Element Seqn Id St Int Rpt
YVWNKRESTEKG Q8VCK8 206 12 -
YVWNKRESTEKG Q9D5V9 206 12 -
YVWNKRESTEKG DAP3_HUMAN 208 12 -
YVWSKRESTPAG Q9W253 204 14 -
YEWSKSERTLKG Q9TVM5 181 15 -

Motif 7 width=15
Element Seqn Id St Int Rpt
EVVEQGLTRVRNATD Q8VCK8 222 4 -
EVVEQGLTRVRNATD Q9D5V9 222 4 -
EVVEQGITRVRNATD DAP3_HUMAN 224 4 -
ELVEHGIARIKYASE Q9W253 220 4 -
EIIEMGISAPFLASD Q9TVM5 197 4 -

Motif 8 width=13
Element Seqn Id St Int Rpt
HLLVAVDGVNALW Q8VCK8 255 18 -
HLLVAVDGVNALW Q9D5V9 255 18 -
HLLVAVDGINALW DAP3_HUMAN 257 18 -
KVMVAIDGFNAFF Q9W253 253 18 -
KVLVAIDDANSLW Q9TVM5 230 18 -

Motif 9 width=18
Element Seqn Id St Int Rpt
PHELLGKEGFNALEPFLP Q8VCK8 323 55 -
PHELLGKEGFNALEPFLP Q9D5V9 323 55 -
PQELLGKEGFDALDPFIP DAP3_HUMAN 325 55 -
PRYLLGKEGFEHLDPFVP Q9W253 322 56 -
PLELFGEEGFEYVEPFVP Q9TVM5 302 59 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
PPRYMMQVKTFGE Q8VCK8 82 82 -
PPRYMMQVKTFGE Q9ER88 77 77 -
PPRYMMQVKTFGE Q9D5V9 82 82 -
PPRFVMQVKTFSE DAP3_HUMAN 84 84 -
PPRFVMQVKTFSE Q13044 84 84 -
PKQYEQQIKTFTE Q9W253 78 78 -
PAPLSKQMDTLGE Q9TVM5 54 54 -

Motif 2 width=12
Element Seqn Id St Int Rpt
RKPALELLGYLK Q8VCK8 100 5 -
RKPALELLGYLK Q9ER88 95 5 -
RKPALELLGYLK Q9D5V9 100 5 -
RKPALELLHYLK DAP3_HUMAN 102 5 -
RKPALELLHYLK Q13044 102 5 -
RNPALELLQYIK Q9W253 96 5 -
REPMAEVASCMR Q9TVM5 72 5 -

Motif 3 width=18
Element Seqn Id St Int Rpt
PAVRYLLYGEKGTGKTLS Q8VCK8 118 6 -
PAVRYLLYGEKGTGKTLS Q9ER88 113 6 -
PAVRYLLYGEKGTGKTLS Q9D5V9 118 6 -
PAIRYLLYGEKGTGKTLS DAP3_HUMAN 120 6 -
PAIRYLLYGEKGTGKTLS Q13044 120 6 -
PVVRYVLYGENGTGKTLT Q9W253 114 6 -
PALRLVLWGAFGTGKSVT Q9TVM5 90 6 -

Motif 4 width=12
Element Seqn Id St Int Rpt
HFCARHDWLILH Q8VCK8 141 5 -
HFCARHDWLILH Q9ER88 136 5 -
HFCARHDWLILH Q9D5V9 141 5 -
HFCAKQDWLILH DAP3_HUMAN 143 5 -
HFCAKQDWLILH Q13044 143 5 -
HYGALNDFLLVH Q9W253 137 5 -
HHAYNNKWAIVH Q9TVM5 113 5 -

Motif 5 width=15
Element Seqn Id St Int Rpt
PLEASTWLKNFKTTN Q8VCK8 179 26 -
PLEASTWLKNFKTTN Q9ER88 174 26 -
PLEGSTWLKNFKTTN Q9D5V9 179 26 -
PLEASTWLKNFKTTN DAP3_HUMAN 181 26 -
PLEASTWLKNFKTTN Q13044 181 26 -
PFDAAAWLVHFKTQN Q9W253 175 26 -
PSNSVSILQNFKQQN Q9TVM5 151 26 -

Motif 6 width=12
Element Seqn Id St Int Rpt
YVWNKRESTEKG Q8VCK8 206 12 -
YVWNKRESTEKG Q9ER88 201 12 -
YVWNKRESTEKG Q9D5V9 206 12 -
YVWNKRESTEKG DAP3_HUMAN 208 12 -
YVWNKRESTEKG Q13044 208 12 -
YVWSKRESTPAG Q9W253 204 14 -
YEWSKSERTLKG Q9TVM5 181 15 -

Motif 7 width=15
Element Seqn Id St Int Rpt
EVVEQGLTRVRNATD Q8VCK8 222 4 -
EVVEQGLTRVRNATD Q9ER88 217 4 -
EVVEQGLTRVRNATD Q9D5V9 222 4 -
EVVEQGITRVRNATD DAP3_HUMAN 224 4 -
EVVEQGITRVRNATD Q13044 224 4 -
ELVEHGIARIKYASE Q9W253 220 4 -
EIIEMGISAPFLASD Q9TVM5 197 4 -

Motif 8 width=13
Element Seqn Id St Int Rpt
HLLVAVDGVNALW Q8VCK8 255 18 -
HLLVAVDGVNALW Q9ER88 250 18 -
HLLVAVDGVNALW Q9D5V9 255 18 -
HLLVAVDGINALW DAP3_HUMAN 257 18 -
HLLVAVDGINALW Q13044 257 18 -
KVMVAIDGFNAFF Q9W253 253 18 -
KVLVAIDDANSLW Q9TVM5 230 18 -

Motif 9 width=18
Element Seqn Id St Int Rpt
PHELLGKEGFNALEPFLP Q8VCK8 323 55 -
PHELLGKEGFNALEPFLP Q9ER88 318 55 -
PHELLGKEGFNALEPFLP Q9D5V9 323 55 -
PQELLGKEGFDALDPFIP DAP3_HUMAN 325 55 -
PQELLGKEGFDALDPFIP Q13044 325 55 -
PRYLLGKEGFEHLDPFVP Q9W253 322 56 -
PLELFGEEGFEYVEPFVP Q9TVM5 302 59 -