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PR01714

Identifier
2FE2SRDCTASE  [View Relations]  [View Alignment]  
Accession
PR01714
No. of Motifs
8
Creation Date
14-JUN-2002
Title
Ferric iron reductase signature 
Database References
Literature References
1. PAYNE, S.M. 
Detection, isolation, and characterisation of siderophores.
METHODS ENZYMOL. 235 329-344 (1994). 
 
2. MULLER, K., MATZANKE, B.F., SCHUNEMANN, V., TRAUTWEIN, A.X. AND HANTKE, K.
FhuF, an iron-regulated protein of Escherichia coli with a new type of 
EUR.J.BIOCHEM. 258 1001-1008 (1998).
 
3. PATZER, S.I. AND HANTKE, K.
SufS is a NifS-like protein, and SufD is necessary for stability of the 
J.BACTERIOL. 181 3307-3309 (1999).
 
4. HANTKE, K.
Members of the Fur protein family regulate iron and zinc transport in E.coli 
and characteristics of the Fur-regulated FhuF protein.
J.MOL.MICROBIOL.BIOTECHNOL. 4 217-222 (2002).

Documentation
Iron is essential for growth in both bacteria and mammals. Controlling the 
amount of free iron in solution is often used as a tactic by hosts to limit 
invasion of pathogenic microbes; binding iron tightly within protein 
molecules can accomplish this. Such iron-protein complexes include haem in 
blood, lactoferrin in tears/saliva, and transferrin in blood plasma. Some 
bacteria express surface receptors to capture eukaryotic iron-binding 
compounds, while others have evolved siderophores (enterobactins) to 
scavenge iron from iron-binding host proteins [1]. 
 
The control of such siderophore gene expression in Escherichia coli is under 
the regulation of the negative repressor protein FUR [2]. When complexed 
with Fe2+, it down-regulates the transcription not only of the siderophore 
genes, but also of the moieties that release Fe2+ ions bound to the hydrox-
amate enterobactin proteins in the microbial cytoplasm [2]. An example of 
the latter is FhuF from the Gram-negative microbes Yersinia pestis, 
Salmonella typhi, and Escherichia coli [2]. In conjunction with the 
siderophore system, this gene has been demonstrated to be essential for 
growth and virulence in pathogenic enterobacteria [2].
 
FhuF is a member of the [2Fe-2S] ferric iron reductase family. However, 
in place of the symmetrical tetrahedral arrangement at the ferric iron
binding site, an unusual Cys-Cys C-terminal group distorts the site in this
protein [3]. This property makes FhuF inherently unstable, and another set
of regulatory genes, designated "suf", is thought to maintain its activity
in the cytoplasm [4].
 
2FE2SRDCTASE is an 8-element fingerprint that provides a signature for 
bacterial ferric iron reductases. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning the full alignment length (~250 amino acids) - motif 8 
spans the ferric iron binding site, which includes 4 cysteine residues. Two 
iterations on SPTR40_20f were required to reach convergence, at which point
a true set comprising 5 sequences was identified. 
Summary Information
5 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
855555555
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
FHUF_ECOLI    Q8XB48        Q8Z0V5        Q8ZFZ5        
Q8ZJW9
Sequence Titles
FHUF_ECOLI  Ferric iron reductase protein fhuF - Escherichia coli. 
Q8XB48 ORF, HYPOTHETICAL PROTEIN (FERRIC IRON REDUCTASE PROTEIN) - Escherichia coli O157:H7.
Q8Z0V5 FERRIC IRON REDUCTASE PROTEIN - Salmonella typhi.
Q8ZFZ5 PUTATIVE FERRIC IRON REDUCTASE - Yersinia pestis.
Q8ZJW9 FERRIC HYDROZAMATE TRANSPORT, INVOLVED IN REDUCTION OF FERRIC IRON IN CYTOPLASMIC FERRIOXAMINE B - Salmonella typhimurium.
Scan History
SPTR40_20f 2  75   NSINGLE    
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
THLQPQDARLAQAVRAT Q8XB48 16 16 -
THLQPQDPTLAQAVRAT FHUF_ECOLI 16 16 -
QLITPSPIGLIADITEL Q8ZFZ5 6 6 -

Motif 2 width=18
Element Seqn Id St Int Rpt
TLAQWSSPNALSSLLAVY Q8XB48 56 23 -
TLAQWSSPNVLSSLLAVY FHUF_ECOLI 56 23 -
SFHTWSSIDNFPTLIQKY Q8ZFZ5 47 24 -

Motif 3 width=16
Element Seqn Id St Int Rpt
SLWAQWYIGLMVPPLM Q8XB48 93 19 -
SLWAQWYIGLMVPPLM FHUF_ECOLI 93 19 -
SLWSQWYFGLIIPPMM Q8ZFZ5 83 18 -

Motif 4 width=15
Element Seqn Id St Int Rpt
FHAEFHETGRVACFW Q8XB48 125 16 -
FHAEFHETGRVACFW FHUF_ECOLI 125 16 -
FKVLFHHSGRPEVVY Q8ZFZ5 115 16 -

Motif 5 width=17
Element Seqn Id St Int Rpt
EATGEINGKLIWSNTGY Q8XB48 173 33 -
EATGEINGKLIWSNTGY FHUF_ECOLI 173 33 -
ESYQGINGRLLWNNIGY Q8ZFZ5 162 32 -

Motif 6 width=16
Element Seqn Id St Int Rpt
EMKQLLGEATVESLRH Q8XB48 197 7 -
EMKQLLGEATVESLRH FHUF_ECOLI 197 7 -
EFKGRLGDDLYQSIIN Q8ZFZ5 186 7 -

Motif 7 width=17
Element Seqn Id St Int Rpt
FFEKTLTTGEDNPLWRT Q8XB48 215 2 -
FFEKTLTNGEDNPLWRT FHUF_ECOLI 215 2 -
FMELSLPNGQDNPLYRT Q8ZFZ5 204 2 -

Motif 8 width=19
Element Seqn Id St Int Rpt
RRTCCQRYRLPDVQQCGDC Q8XB48 241 9 -
RRTCCQRYRLPDVQQCGDC FHUF_ECOLI 241 9 -
RRSCCQRNKLPGVRSCHDC Q8ZFZ5 230 9 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
AAPQPEDASLADAVRET Q8Z0V5 16 16 -
AAPQPEDASLADAVRET Q8ZJW9 16 16 -
THLQPQDARLAQAVRAT Q8XB48 16 16 -
THLQPQDPTLAQAVRAT FHUF_ECOLI 16 16 -
QLITPSPIGLIADITEL Q8ZFZ5 6 6 -

Motif 2 width=18
Element Seqn Id St Int Rpt
TLAQWTRPATFRSLLAAY Q8Z0V5 56 23 -
TLAQWTRPATFRSLLAAY Q8ZJW9 56 23 -
TLAQWSSPNALSSLLAVY Q8XB48 56 23 -
TLAQWSSPNVLSSLLAVY FHUF_ECOLI 56 23 -
SFHTWSSIDNFPTLIQKY Q8ZFZ5 47 24 -

Motif 3 width=16
Element Seqn Id St Int Rpt
SLWAQWYIGLMTPPLM Q8Z0V5 93 19 -
SLWAQWYIGLMTPPLM Q8ZJW9 93 19 -
SLWAQWYIGLMVPPLM Q8XB48 93 19 -
SLWAQWYIGLMVPPLM FHUF_ECOLI 93 19 -
SLWSQWYFGLIIPPMM Q8ZFZ5 83 18 -

Motif 4 width=15
Element Seqn Id St Int Rpt
IHVEFHETGRAACFW Q8Z0V5 125 16 -
IHVEFHETGRAACFW Q8ZJW9 125 16 -
FHAEFHETGRVACFW Q8XB48 125 16 -
FHAEFHETGRVACFW FHUF_ECOLI 125 16 -
FKVLFHHSGRPEVVY Q8ZFZ5 115 16 -

Motif 5 width=17
Element Seqn Id St Int Rpt
EATGDINAKLIWSNTGY Q8Z0V5 173 33 -
EATGDINAKLIWSNTGY Q8ZJW9 173 33 -
EATGEINGKLIWSNTGY Q8XB48 173 33 -
EATGEINGKLIWSNTGY FHUF_ECOLI 173 33 -
ESYQGINGRLLWNNIGY Q8ZFZ5 162 32 -

Motif 6 width=16
Element Seqn Id St Int Rpt
EMKPLLGEALLATLRQ Q8Z0V5 197 7 -
EMKPLLGEALLATLRQ Q8ZJW9 197 7 -
EMKQLLGEATVESLRH Q8XB48 197 7 -
EMKQLLGEATVESLRH FHUF_ECOLI 197 7 -
EFKGRLGDDLYQSIIN Q8ZFZ5 186 7 -

Motif 7 width=17
Element Seqn Id St Int Rpt
FFEKQLSDGQDNPLWRT Q8Z0V5 215 2 -
FFEKQLSDGQDNPLWRT Q8ZJW9 215 2 -
FFEKTLTTGEDNPLWRT Q8XB48 215 2 -
FFEKTLTNGEDNPLWRT FHUF_ECOLI 215 2 -
FMELSLPNGQDNPLYRT Q8ZFZ5 204 2 -

Motif 8 width=19
Element Seqn Id St Int Rpt
RRTCCQRYRLPDVQQCGDC Q8Z0V5 241 9 -
RRTCCQRYRLPDVQQCGDC Q8ZJW9 241 9 -
RRTCCQRYRLPDVQQCGDC Q8XB48 241 9 -
RRTCCQRYRLPDVQQCGDC FHUF_ECOLI 241 9 -
RRSCCQRNKLPGVRSCHDC Q8ZFZ5 230 9 -