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PR01700

Identifier
CD34ANTIGEN  [View Relations]  [View Alignment]  
Accession
PR01700
No. of Motifs
8
Creation Date
20-JAN-2002
Title
CD34 antigen signature
Database References
Literature References
1. FACKLERT, M.J., CIVINS, C.I., SUTHERLAND, D.R., BAKER, M.A. AND MAY, W.S.
Activated protein kinase C directly phosphorylates the CD34 antigen on 
hemopoietic cells.
J.BIOL.CHEM. 265 11056-11061 (1990).
 
2. BROWN, J., G REAVES, M.F. AND MOLGAARD, H.V.
The gene encoding the stem cell antigen CD34 is conserved in mouse and 
expressed in haemopoietic progenitor cell lines, brain and embryonic 
fibroblasts.
INT.IMMUNOL. 3 175-184 (1991).

Documentation
The CD34 group of monoclonal antibodies recognises CD34 (also termed CD34
antigen), a 105-120kDa cell surface glycoprotein, which is selectively 
expressed by human myeloid and lymphoid progenitor cells, including 
the haemopoietic stem cell. The protein is also expressed on vascular
endothelial cells. Here, it is concentrated on the surface of the inter-
digitating processes, suggesting a possible involvement in cell interactions
or adhesion, by mediating the attachment of stem cells to the bone marrow 
extracellular matrix, or directly to stromal cells. The restricted pattern
of expression of CD34 in haemopoiesis suggests that it may have a 
significant function in the earliest stages of blood cell differentiation 
in the bone marrow [1,2]. 
 
CD34 is a phosphoprotein shown to be activated by protein kinase C (PKC) in
a developmental stage-specific manner. Analysis of the human CD34 sequence
reveals that the protein appears to be a type I transmembrane (TM) molecule.
The predicted internal portion of the protein appears to retain basic amino 
acid residues adjacent to Ser residues, presenting at least two potential
target sites for PKC phosphorylation. In addition, there are two other 
consensus motifs that correspond to potential target sites for 
Ca+/calmodulin-dependent kinase and/or protease activated kinase I [1].
 
The protein is not strongly similar to other known proteins, but some weak
similarities do exist: e.g., to the S+T region (a region rich in potential
O-linked carbohydrate attachment sites), the TM domain and cytoplasmic 
domain of cell surface proteins such as leukosialin, a major sialoglyco-
protein of rat and human leukocytes; to the N-terminal glycosylated region
of CD45 (the leukocyte common antigen); and to groups of interrelated
proteins involved in cell adhesion or the regulation of complement.
 
A homologue of human CD34 is expressed in mouse. The amino acid sequences
only diverge significantly at their N-termini, which are predicted to be 
highly glycosylated and whose functions are probably modulated by 
carbohydrate. The observed pattern of expression of the murine CD34 gene
is consistent with that of the human antigen. That CD34 is also highly
expressed outside haematopoiesis, by vascular endothelial cells and by 
fibroblasts in differentiated tissue, suggests a role common to a variety
of cell types. Concentration of CD34 on the interdigitating membrane
projections of adjacent capillary endothelial cells has strengthened the
idea that it functions in the control of events leading to cell-cell or
cell-matrix adhesion, which role could be modulated by variation in its
levels of glycosylation. The conservation between the human and mouse
cysteine-rich domain in the extracellular part of the protein, and the
exceptionally high conservation of the cytoplasmic domain, imply that the
protein is more than a carrier for either carbohydrate or negatively charged
terminal sialic acid residues (a role postulated for leukosialin/sialophorin).
The highly conserved domain may serve to provide an internal signal of 
external contact with a ligand [1].
 
CD34ANTIGEN is an 8-element fingerprint that provides a signature for the 
CD34 haematopoietic stem cell antigens. The fingerprint was derived from an
initial alignment of 5 sequences: the motifs were drawn from conserved
regions spanning the C-terminal portion of the alignment - motifs 4 and 5
span the N- and C-terminal regions of the predicted TM domain; and motifs 7
and 8 contain putative N-glycosylation sites. A single iteration on
SPTR39_17f was required to reach convergence, no further sequences being
identified beyond the starting set. 
Summary Information
5 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
855555555
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
CD34_CANFA    CD34_HUMAN    CD34_MOUSE    Q9UJB1        
Q9XS61
Sequence Titles
CD34_CANFA  HEMATOPOIETIC PROGENITOR CELL ANTIGEN CD34 PRECURSOR - Canis familiaris (Dog). 
CD34_HUMAN HEMATOPOIETIC PROGENITOR CELL ANTIGEN CD34 PRECURSOR - Homo sapiens (Human).
CD34_MOUSE HEMATOPOIETIC PROGENITOR CELL ANTIGEN CD34 PRECURSOR - Mus musculus (Mouse).
Q9UJB1 CD34 (HEMATOPOIETIC STEM CELL SURFACE ANTIGEN) - Homo sapiens (Human).
Q9XS61 CD34 - Bos taurus (Bovine).
Scan History
SPTR39_17f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
EIKCSGIREVK CD34_HUMAN 174 174 -
EIKCSGIREVK Q9UJB1 162 162 -
EIKCSGVKEVK CD34_CANFA 180 180 -
EIKCSRVKEVK Q9XS61 177 177 -
EIKCSGIREVR CD34_MOUSE 171 171 -

Motif 2 width=9
Element Seqn Id St Int Rpt
TSSCAEFKK CD34_HUMAN 196 11 -
TSSCAEFKK Q9UJB1 184 11 -
TSSCEDFKK CD34_CANFA 202 11 -
TSSCEEFKK Q9XS61 199 11 -
ASSCEEFKK CD34_MOUSE 193 11 -

Motif 3 width=12
Element Seqn Id St Int Rpt
CSLLLAQSEVRP CD34_HUMAN 229 24 -
CSLLLAQSEVRP Q9UJB1 217 24 -
CSLLLAQSEVRP CD34_CANFA 233 22 -
CSLLLAQSEVRP Q9XS61 226 18 -
CSLLLAQSEVRP CD34_MOUSE 226 24 -

Motif 4 width=17
Element Seqn Id St Int Rpt
SHQSYSQKTLIALVTSG CD34_HUMAN 282 41 -
SHQSYSQKTLIALVTSG Q9UJB1 270 41 -
SHQSYSRKTLIALVTSG CD34_CANFA 286 41 -
SHQSYSRKTLIALVTSG Q9XS61 279 41 -
SHQSYSRKTLIALVTSG CD34_MOUSE 279 41 -

Motif 5 width=22
Element Seqn Id St Int Rpt
YFLMNRRSWSPTGERLGEDPYY CD34_HUMAN 309 10 -
YFLMNRRSWSPTGERLGEDPYY Q9UJB1 297 10 -
YFLMNRRSWSPTGERLGEDPYY CD34_CANFA 313 10 -
YFLMNRRSWSPTGERLGEDPYY Q9XS61 306 10 -
YFLMNRRSWSPTGERLGEDPYY CD34_MOUSE 306 10 -

Motif 6 width=12
Element Seqn Id St Int Rpt
ENGGGQGYSSGP CD34_HUMAN 332 1 -
ENGGGQGYSSGP Q9UJB1 320 1 -
ENGGGQGYSSGP CD34_CANFA 336 1 -
ENGGGQGYSSGP Q9XS61 329 1 -
ENGGGQGYSSGP CD34_MOUSE 329 1 -

Motif 7 width=13
Element Seqn Id St Int Rpt
SPEAQGKASVNRG CD34_HUMAN 346 2 -
SPEAQGKASVNRG Q9UJB1 334 2 -
SPEAQGKASVNRG CD34_CANFA 350 2 -
SPEAQGKAIVNRG Q9XS61 343 2 -
SPETQGKANVTRG CD34_MOUSE 343 2 -

Motif 8 width=25
Element Seqn Id St Int Rpt
QENGTGQATSRNGHSARQHVVADTE CD34_HUMAN 360 1 -
QENGTGQATSRNGHSARQHVVADTE Q9UJB1 348 1 -
QENGTGQATSRNGHSARQHMVADTE CD34_CANFA 364 1 -
QENGTGQATSRNGHSARQRVVADTE Q9XS61 357 1 -
QENGTGQATSRNGHSARQHVVADTE CD34_MOUSE 357 1 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
EIKCSGIREVK CD34_HUMAN 174 174 -
EIKCSGIREVK Q9UJB1 162 162 -
EIKCSGVKEVK CD34_CANFA 180 180 -
EIKCSRVKEVK Q9XS61 177 177 -
EIKCSGIREVR CD34_MOUSE 171 171 -

Motif 2 width=9
Element Seqn Id St Int Rpt
TSSCAEFKK CD34_HUMAN 196 11 -
TSSCAEFKK Q9UJB1 184 11 -
TSSCEDFKK CD34_CANFA 202 11 -
TSSCEEFKK Q9XS61 199 11 -
ASSCEEFKK CD34_MOUSE 193 11 -

Motif 3 width=12
Element Seqn Id St Int Rpt
CSLLLAQSEVRP CD34_HUMAN 229 24 -
CSLLLAQSEVRP Q9UJB1 217 24 -
CSLLLAQSEVRP CD34_CANFA 233 22 -
CSLLLAQSEVRP Q9XS61 226 18 -
CSLLLAQSEVRP CD34_MOUSE 226 24 -

Motif 4 width=17
Element Seqn Id St Int Rpt
SHQSYSQKTLIALVTSG CD34_HUMAN 282 41 -
SHQSYSQKTLIALVTSG Q9UJB1 270 41 -
SHQSYSRKTLIALVTSG CD34_CANFA 286 41 -
SHQSYSRKTLIALVTSG Q9XS61 279 41 -
SHQSYSRKTLIALVTSG CD34_MOUSE 279 41 -

Motif 5 width=22
Element Seqn Id St Int Rpt
YFLMNRRSWSPTGERLGEDPYY CD34_HUMAN 309 10 -
YFLMNRRSWSPTGERLGEDPYY Q9UJB1 297 10 -
YFLMNRRSWSPTGERLGEDPYY CD34_CANFA 313 10 -
YFLMNRRSWSPTGERLGEDPYY Q9XS61 306 10 -
YFLMNRRSWSPTGERLGEDPYY CD34_MOUSE 306 10 -

Motif 6 width=12
Element Seqn Id St Int Rpt
ENGGGQGYSSGP CD34_HUMAN 332 1 -
ENGGGQGYSSGP Q9UJB1 320 1 -
ENGGGQGYSSGP CD34_CANFA 336 1 -
ENGGGQGYSSGP Q9XS61 329 1 -
ENGGGQGYSSGP CD34_MOUSE 329 1 -

Motif 7 width=13
Element Seqn Id St Int Rpt
SPEAQGKASVNRG CD34_HUMAN 346 2 -
SPEAQGKASVNRG Q9UJB1 334 2 -
SPEAQGKASVNRG CD34_CANFA 350 2 -
SPEAQGKAIVNRG Q9XS61 343 2 -
SPETQGKANVTRG CD34_MOUSE 343 2 -

Motif 8 width=25
Element Seqn Id St Int Rpt
QENGTGQATSRNGHSARQHVVADTE CD34_HUMAN 360 1 -
QENGTGQATSRNGHSARQHVVADTE Q9UJB1 348 1 -
QENGTGQATSRNGHSARQHMVADTE CD34_CANFA 364 1 -
QENGTGQATSRNGHSARQRVVADTE Q9XS61 357 1 -
QENGTGQATSRNGHSARQHVVADTE CD34_MOUSE 357 1 -