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PR01692

Identifier
LIPOCALINIMR  [View Relations]  [View Alignment]  
Accession
PR01692
No. of Motifs
7
Creation Date
06-FEB-2002
Title
Lipocalin-1 interacting membrane receptor signature
Database References
Literature References
1. REDL, B., HOLZFEIND P. AND LOTTSPEICH F.
cDNA cloning and sequencing reveals human tear prealbumin to be a member of the 
lipophilic-ligand carrier protein superfamily.
J.BIOL.CHEM. 267 20282-20287 (1992).
 
2. REDL, B.
Human tear lipocalin.
BIOCHIM.BIOPHYS.ACTA 1482 241-248 (2000).
 
3. WOJNAR, P., LECHNER, M., MERSCHAK, P. AND REDL, B.
Molecular cloning of a novel lipocalin-1 interacting human cell membrane 
receptor using phage display. 
J.BIOL.CHEM. 276 20206-20212 (2001).
 
4. FLOWER, D.R.
Beyond the superfamily: the lipocalin receptors.
BIOCHIM.BIOPHYS.ACTA 1482 327-336 (2000).

Documentation
Lipocalin-1 (Lcn-1, tear lipocalin or von Ebners gland protein) is a member
of the lipocalin superfamily produced by a number of secretory glands and
tissues [2]. The biological relevance of the many possible activities of
Lcn-1 has still to be established, but its main function appears to be
scavenging of lipophilic, potentially harmful molecules, providing a
protection factor for cells and tissues [3]. 
 
Beyond its interaction with Lcn-1, the precise physiological function of
lipocalin-1 interacting membrane receptor (LIMR) is unknown. It could be
involved in the detoxification of the ligands bound to Lcn-1, either
transferring the ligand into the cell, or acting as a detoxification
protein itself, with possible enzymatic activity similar to the membrane-
associated enzymes involved in lipid molecule modification [1]. 
 
Currently, there is only limited data available concerning the identity of 
lipocalin receptors. While there is clear evidence for a specific receptor
for plasma retinol binding protein (RBP), there is only indirect evidence of
receptors for alpha1-microglobulin (A1M), major urinary protein (MUP), 
beta-lactoglobulin, glycodelin, insecticyanin, alpha1-acid glycoprotein 
(AGP) and odorant binding protein (OBP). There is no consensus in the
mechanism of interaction between lipocalins and their receptors. Lipocalin
receptors have been found to act via carbohydrate binding and protein-
protein interactions. With the exception of megalin, an apparent endocytic
receptor for several soluble macromolecules including some lipocalins, no
specific lipocalin receptor has so far been fully characterised [4].
 
LIPOCALINIMR is a 7-element fingerprint that provides a signature for
lipocalin-1 interacting membrane receptors. The fingerprint was derived from
an initial alignment of 5 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length. Two iterations on
SPTR39_18f were required to reach convergence, at which a true set
comprising 8 sequences was identified. Three partial matches were also found:
Q9HAG9 is a human hypothetical protein that matches motifs 1-3; and Q9H5N0
is another human sequence, which matches the C-terminal two motifs.
Summary Information
   8 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
78888888
60000000
50000000
40000000
31110000
20000011
1234567
True Positives
O73804        Q9D1E5        Q9JIT0        Q9NVE1        
Q9NVU9 Q9VC35 Q9XZ04 YNC2_CAEEL
True Positive Partials
Codes involving 3 elements
Q9HAG9
Codes involving 2 elements
Q9H5N0
Sequence Titles
O73804      HYPOTHETICAL 56.4 KDA PROTEIN - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes). 
Q9D1E5 1110013E13RIK PROTEIN - Mus musculus (Mouse).
Q9JIT0 LMBR1 LONG FORM - Mus musculus (Mouse).
Q9NVE1 CDNA FLJ10789 FIS, CLONE NT2RP4000500 - Homo sapiens (Human).
Q9NVU9 CDNA FLJ10494 FIS, CLONE NT2RP2000283 - Homo sapiens (Human).
Q9VC35 BCDNA:GH12663 PROTEIN - Drosophila melanogaster (Fruit fly).
Q9XZ04 HYPOTHETICAL 60.1 KDA PROTEIN - Drosophila melanogaster (Fruit fly).
YNC2_CAEEL Hypothetical 83.6 kDa protein R05D3.2 in chromosome III - Caenorhabditis elegans.

Q9HAG9 HYPOTHETICAL 25.8 KDA PROTEIN - Homo sapiens (Human).

Q9H5N0 CDNA: FLJ23287 FIS, CLONE HEP09714 (DIF14) - Homo sapiens (Human).
Scan History
SPTR39_18f 2  75   NSINGLE    
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
FTLAIALGAVLLLPFSIISNEVL Q9NVE1 70 70 -
FTLAIALGAVLLLPFSIISNEVL Q9NVU9 65 65 -
FTLSVAVCAVLLLPISILSNEVL O73804 81 81 -
FTLAVSAGAVLLLPFSIISNEIL Q9JIT0 66 66 -

Motif 2 width=23
Element Seqn Id St Int Rpt
LWNLVFLFSNLSLIFLMPFAYFF Q9NVE1 112 19 -
LWNLVFLFSNLSLIFLMPFAYFF Q9NVU9 107 19 -
LWNLVFLFSNLSLVFLMPFAYFF O73804 123 19 -
LWNLASLFSNLCLFVLMPFAFFF Q9JIT0 108 19 -

Motif 3 width=22
Element Seqn Id St Int Rpt
YYLPYLYSCISFLGVLLLLVCT Q9NVE1 192 57 -
YYLPYLYSCISFLGVLLLLVCT Q9NVU9 187 57 -
YYLPYLYSGISLFGVLLLLLCT O73804 202 56 -
FYLPYLYSCISLMGCLLLLLCT Q9JIT0 188 57 -

Motif 4 width=22
Element Seqn Id St Int Rpt
YPLAMLCLLVLTGLSVLIVAIH Q9NVE1 299 85 -
YPLAMLCLLVLTGLSVLIVAIH Q9NVU9 294 85 -
YPLAMLTLLALTVMCVLMVCIN O73804 313 89 -
YPAVMVLLLIETSISVLLVACN Q9JIT0 294 84 -

Motif 5 width=21
Element Seqn Id St Int Rpt
VVLIFYLMVSSVVGFYSSPLF Q9NVE1 337 16 -
VVLIFYLMVSSVVGFYSSPLF Q9NVU9 352 36 -
VLLILYLMVSSVVGFYSSPFF O73804 373 38 -
IILIFYLMVSSVVGFYSLRFF Q9JIT0 352 36 -

Motif 6 width=22
Element Seqn Id St Int Rpt
TAMTQIIGNCVCLLVLSSALPV Q9NVE1 367 9 -
TAMTQIIGNCVCLLVLSSALPV Q9NVU9 382 9 -
TNLTQIIANCMSLLILSSALPV O73804 403 9 -
TTMTKIIGNCVSILVLSSALPV Q9JIT0 382 9 -

Motif 7 width=24
Element Seqn Id St Int Rpt
WLGNFYIVFLYNAAFAGLTTLCLV Q9NVE1 409 20 -
WLGNFYIVFLYNAAFAGLTTLCLV Q9NVU9 424 20 -
WLGNFYVVFLYNMMFAGLTSASLI O73804 445 20 -
WLGNFYIVLSYNLLFAIMTTLCLI Q9JIT0 424 20 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
FTLAVALGAVLLLPFSIISNEVL Q9D1E5 70 70 -
FTLAIALGAVLLLPFSIISNEVL Q9NVE1 70 70 -
FTLAIALGAVLLLPFSIISNEVL Q9NVU9 65 65 -
FTLSVAVCAVLLLPISILSNEVL O73804 81 81 -
FTLAVAEGAAMLLPVSIASNEVL Q9VC35 73 73 -
FTLAVAEGAAMLLPVSIASNEVL Q9XZ04 73 73 -
FTLAVSAGAVLLLPFSIISNEIL Q9JIT0 66 66 -
TATATSIGSLTLLPFSVIGVELL YNC2_CAEEL 118 118 -

Motif 2 width=23
Element Seqn Id St Int Rpt
LWNLVFLFSNLSLVFLMPFAYFF Q9D1E5 112 19 -
LWNLVFLFSNLSLIFLMPFAYFF Q9NVE1 112 19 -
LWNLVFLFSNLSLIFLMPFAYFF Q9NVU9 107 19 -
LWNLVFLFSNLSLVFLMPFAYFF O73804 123 19 -
LWNHVFLFSNLSLFIFLPFVYLF Q9VC35 115 19 -
LWNHVFLFSNLSLFIFLPFVYLF Q9XZ04 115 19 -
LWNLASLFSNLCLFVLMPFAFFF Q9JIT0 108 19 -
LWNYVFVLSNVSLFVLLPFSYFF YNC2_CAEEL 160 19 -

Motif 3 width=22
Element Seqn Id St Int Rpt
YYLPYLYSCISFLGVLLLLVCT Q9D1E5 192 57 -
YYLPYLYSCISFLGVLLLLVCT Q9NVE1 192 57 -
YYLPYLYSCISFLGVLLLLVCT Q9NVU9 187 57 -
YYLPYLYSGISLFGVLLLLLCT O73804 202 56 -
VHLPFLYSCVSFLGVMLMLICT Q9VC35 195 57 -
VHLPFLYSCVSFLGVMLMLICT Q9XZ04 195 57 -
FYLPYLYSCISLMGCLLLLLCT Q9JIT0 188 57 -
VNLPLIYSCVSFIGAVLLLIST YNC2_CAEEL 238 55 -

Motif 4 width=22
Element Seqn Id St Int Rpt
YPLAMLCLLVLTGLSVLIVAVH Q9D1E5 299 85 -
YPLAMLCLLVLTGLSVLIVAIH Q9NVE1 299 85 -
YPLAMLCLLVLTGLSVLIVAIH Q9NVU9 294 85 -
YPLAMLTLLALTVMCVLMVCIN O73804 313 89 -
YPLAMLLLLFCTAVTILLVVQN Q9VC35 344 127 -
YPLAMLLLLFCTAVTILLVVQN Q9XZ04 344 127 -
YPAVMVLLLIETSISVLLVACN Q9JIT0 294 84 -
FPVAAAALLVLTTCSLIMVATN YNC2_CAEEL 544 284 -

Motif 5 width=21
Element Seqn Id St Int Rpt
VVLIFYLMVSSVVGFYSSPLF Q9D1E5 357 36 -
VVLIFYLMVSSVVGFYSSPLF Q9NVE1 337 16 -
VVLIFYLMVSSVVGFYSSPLF Q9NVU9 352 36 -
VLLILYLMVSSVVGFYSSPFF O73804 373 38 -
VCLIFYLGATSVVGFYSMPFM Q9VC35 402 36 -
VRLIFYLGATSVVGFYSMPFM Q9XZ04 402 36 -
IILIFYLMVSSVVGFYSLRFF Q9JIT0 352 36 -
TLLIIYVMITSFVGLYSLPVL YNC2_CAEEL 600 34 -

Motif 6 width=22
Element Seqn Id St Int Rpt
TSMTQIIGNCVCLLVLSSALPV Q9D1E5 387 9 -
TAMTQIIGNCVCLLVLSSALPV Q9NVE1 367 9 -
TAMTQIIGNCVCLLVLSSALPV Q9NVU9 382 9 -
TNLTQIIANCMSLLILSSALPV O73804 403 9 -
TSLPQLMLNCGFMLVLSSALPL Q9VC35 432 9 -
TSLPQLMLNCGFMLVLSSALPL Q9XZ04 432 9 -
TTMTKIIGNCVSILVLSSALPV Q9JIT0 382 9 -
TPMPTIIINSSIVLVVASALPV YNC2_CAEEL 630 9 -

Motif 7 width=24
Element Seqn Id St Int Rpt
WLGNFYIVFLYNAAFAGLTTLCLV Q9D1E5 429 20 -
WLGNFYIVFLYNAAFAGLTTLCLV Q9NVE1 409 20 -
WLGNFYIVFLYNAAFAGLTTLCLV Q9NVU9 424 20 -
WLGNFYVVFLYNMMFAGLTSASLI O73804 445 20 -
WLGNFQIVLLYNLVFGTTTALCLA Q9VC35 474 20 -
WLGNFQIVLLYNLVFGTTTALCLA Q9XZ04 474 20 -
WLGNFYIVLSYNLLFAIMTTLCLI Q9JIT0 424 20 -
WLGSFRVVVAYNTLFVVLSVAFLF YNC2_CAEEL 672 20 -