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PR01647

Identifier
TRPCHANNEL6  [View Relations]  [View Alignment]  
Accession
PR01647
No. of Motifs
5
Creation Date
13-NOV-2001
Title
Transient receptor potential channel 6 signature
Database References
PRINTS; PR01097 TRNSRECEPTRP
MIM; 603652
Literature References
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E. 
AND BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated 
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
 
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E. 
AND BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient
receptor potential (Trp) involved in calcium entry secondary to activation 
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).
 
3. CLAPHAM, D., RUNNELS, L. AND STRUBING, C.
The TRP ion channel family.
NAT.REV.NEUROSCI. 2 6387-396 (2001). 
 
4. HARTENECK, C., PLANT, T. AND SCHULTZ, G.
From worm to man: three subfamilies of TRP channels.
TRENDS NEUROSCI. 23 159-166 (2000).
 
5. HOFMANN, T., OBUKHOV, A.G., SCHAEFER, M., HARTENECK, C., GUDERMANN, T. 
AND SCHULTZ, G. 
Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. 
NATURE 397 259-263 (1999).
 
6. INOUE, R., OKADA, T., ONOUE, H., HARA, Y., SHIMIZU, S., NAITOH, S., 
ITO, Y. AND MORI Y.
The transient receptor potential protein homologue TRP6 is the essential
component of vascular alpha(1)-adrenoceptor-activated Ca(2+)-permeable 
cation channel.
CIRC.RES. 88 325-232 (2001).

Documentation
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response 
to a range of external and internal cell stimuli. Such Ca2+ entry is thought 
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other 
agents. In non-excitable cells, i.e. those that lack voltage-gated Ca2+
channels, such as hepatocytes, this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
 
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and the cDNA of a related protein, Trp-like, which show
similarity to voltage gated Ca2+ channels in the regions known as S3 through
S6, including the S5-S6 linker that forms the ion-selective channel pore [2].
This provided evidence that Trp and/or related proteins might form mammalian
capacitative Ca2+ entry channels. 
 
A number of Trp and Trp-like channel gene isoforms have now been cloned, 
including several mammalian homologues. The Trp family is thought to encode
at least 20 Ca2+-permeable channel proteins. Hydropathy analysis suggests 
that they share a common transmembrane (TM) topology. Each family member is 
predicted to possess 6 TM domains with intracellular N- and C-termini, which
is similar to the core structure of the pore-forming subunits of the voltage
-gated Na+ and Ca2+ channels. By analogy with these proteins, which have 
4 linked domains of 6 TM segments, it is likely that Trp channels are
homo- or heterotetramers of 4 single subunits [3].
 
The Trp family can be divided on the basis of sequence similarity into 3
subfamilies: short (S), long (L) and osm-like (O) Trp channels [4]. The
STrp subfamily includes Drosophila Trp and Trpl-like, and the mammalian
homologues TrpC1-7. Channels of the STrpC subfamily are activated following
receptor-mediated stimulation of different isoforms of phospholipase C [4].
 
TrpC6 was originally cloned from mouse tissue [2]; human, rat and guinea pig
isoforms have also been identified. Expression studies have demonstrated
that TrpC6 displays low selectivity for Ca2+ over Na+, and, like TrpC3 and 
TrpC7, is activated by diacylglycerol in a protein kinase C-independent
manner [5]. TrpC6 has recently been shown to be an essential component of 
the alpha1-adrenoceptor-activated cation channel [6].
 
TRPCHANNEL6 is a 5-element fingerprint that provides a signature for
transient receptor potential channel 6. The fingerprint was derived from an
initial alignment of 6 sequences: the motifs were drawn from conserved 
regions in the loop and C-terminal portions of the alignment, focusing on 
those sections that characterise TrpC6 but distinguish it from other family
members - motifs 1 and 2 lie in the second extracellular loop; and motifs
3-5 reside within the cytoplasmic C-terminal region. A single iteration on
SPTR40_18f was required to reach convergence, no further sequences being
identified beyond the starting set. A single partial match was found, 
Q9ESX8, a splice variant of the guinea pig TrpC6, which lacks a portion 
of the C-terminal section. 
Summary Information
   6 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
566666
400000
300000
211000
12345
True Positives
Q61143        Q99N76        Q99N77        Q99N78        
Q9ESX6 TRP6_HUMAN
True Positive Partials
Codes involving 2 elements
Q9ESX8
Sequence Titles
Q61143      CALCIUM ENTRY CHANNEL - MUS MUSCULUS (MOUSE). 
Q99N76 TRANSIENT RECEPTOR POTENTIAL CA2+ CHANNEL 6C - Rattus norvegicus (Rat).
Q99N77 TRANSIENT RECEPTOR POTENTIAL CA2+ CHANNEL 6B - Rattus norvegicus (Rat).
Q99N78 TRANSIENT RECEPTOR POTENTIAL CA2+ CHANNEL 6A - Rattus norvegicus (Rat).
Q9ESX6 PUTATIVE CAPACITATIVE CALCIUM ENTRY CHANNEL - Cavia porcellus (Guinea pig).
TRP6_HUMAN Short transient receptor potential channel 6 (TrpC6) - Homo sapiens (Human).

Q9ESX8 TRPC6 CATION CHANNEL, PUTATIVE ALTERNATIVE SPLICE VARIANT GPTRPC6A - Cavia porcellus (Guinea pig).
Scan History
SPTR40_18f 1  200  NSINGLE    
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
WHASKAQSIIDANDTLKDLTKV Q99N76 494 494 -
WHASKAQSIIDANDTLKDLTKV Q99N78 548 548 -
WHASKAQSIIDANDTLKDLTKV Q99N77 494 494 -
WHASKAQSIIDANDTLKDLTKV Q61143 548 548 -
WHASKAQSIIDANDTLKDLTKV TRP6_HUMAN 549 549 -
WHASKAQSIIDANDTLKDLTKV Q9ESX6 511 511 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GDNVKYYNLARIK Q99N76 518 2 -
GDNVKYYNLARIK Q99N78 572 2 -
GDNVKYYNLARIK Q99N77 518 2 -
GDNVKYYNLARIK Q61143 572 2 -
GDNVKYYNLARIK TRP6_HUMAN 573 2 -
GEDVKYYNLARIK Q9ESX6 535 2 -

Motif 3 width=30
Element Seqn Id St Int Rpt
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N76 681 150 -
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N78 803 218 -
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N77 749 218 -
RNEEKKFGISGSHEDLSKFSLDKNQLAHNK Q61143 803 218 -
INEEKKLGILGSHEDLSKLSLDKKQVGHNK TRP6_HUMAN 804 218 -
RNEEKKFGILGSHEDLSKLSVDKKQLGQNK Q9ESX6 766 218 -

Motif 4 width=20
Element Seqn Id St Int Rpt
STRSSEDFHLNSFSNPPRQY Q99N76 713 2 -
STRSSEDFHLNSFSNPPRQY Q99N78 835 2 -
STRSSEDFHLNSFSNPPRQY Q99N77 781 2 -
STRSSEDYHLNSFSNPPRQY Q61143 835 2 -
SIRSSEDFHLNSFNNPPRQY TRP6_HUMAN 836 2 -
SIRSSEDFHLNSFNNPPRQY Q9ESX6 798 2 -

Motif 5 width=15
Element Seqn Id St Int Rpt
GERLSLESKQEESRR Q99N76 794 61 -
GERLSLESKQEESRR Q99N78 916 61 -
GERLSLESKQEESRR Q99N77 862 61 -
GERLSLEPKLEESRR Q61143 916 61 -
GEKLSMEPNQEETNR TRP6_HUMAN 917 61 -
GEKLSSEPKQEEINR Q9ESX6 879 61 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
WHASKAQSIIDANDTLKDLTKV Q99N76 494 494 -
WHASKAQSIIDANDTLKDLTKV Q99N78 548 548 -
WHASKAQSIIDANDTLKDLTKV Q99N77 494 494 -
WHASKAQSIIDANDTLKDLTKV Q61143 548 548 -
WHASKAQSIIDANDTLKDLTKV TRP6_HUMAN 549 549 -
WHASKAQSIIDANDTLKDLTKV Q9ESX6 511 511 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GDNVKYYNLARIK Q99N76 518 2 -
GDNVKYYNLARIK Q99N78 572 2 -
GDNVKYYNLARIK Q99N77 518 2 -
GDNVKYYNLARIK Q61143 572 2 -
GDNVKYYNLARIK TRP6_HUMAN 573 2 -
GEDVKYYNLARIK Q9ESX6 535 2 -

Motif 3 width=30
Element Seqn Id St Int Rpt
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N76 681 150 -
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N78 803 218 -
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N77 749 218 -
RNEEKKFGISGSHEDLSKFSLDKNQLAHNK Q61143 803 218 -
INEEKKLGILGSHEDLSKLSLDKKQVGHNK TRP6_HUMAN 804 218 -
RNEEKKFGILGSHEDLSKLSVDKKQLGQNK Q9ESX6 766 218 -

Motif 4 width=20
Element Seqn Id St Int Rpt
STRSSEDFHLNSFSNPPRQY Q99N76 713 2 -
STRSSEDFHLNSFSNPPRQY Q99N78 835 2 -
STRSSEDFHLNSFSNPPRQY Q99N77 781 2 -
STRSSEDYHLNSFSNPPRQY Q61143 835 2 -
SIRSSEDFHLNSFNNPPRQY TRP6_HUMAN 836 2 -
SIRSSEDFHLNSFNNPPRQY Q9ESX6 798 2 -

Motif 5 width=15
Element Seqn Id St Int Rpt
GERLSLESKQEESRR Q99N76 794 61 -
GERLSLESKQEESRR Q99N78 916 61 -
GERLSLESKQEESRR Q99N77 862 61 -
GERLSLEPKLEESRR Q61143 916 61 -
GEKLSMEPNQEETNR TRP6_HUMAN 917 61 -
GEKLSSEPKQEEINR Q9ESX6 879 61 -