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PR01644

Identifier
TRPCHANNEL3  [View Relations]  [View Alignment]  
Accession
PR01644
No. of Motifs
5
Creation Date
13-NOV-2001
Title
Transient receptor potential channel 3 signature
Database References
PRINTS; PR01097 TRNSRECEPTRP
PRODOM; PD406008
MIM; 602345
Literature References
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E. 
AND BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated 
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
 
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E. 
AND BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient
receptor potential (Trp) involved in calcium entry secondary to activation 
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).
 
3. CLAPHAM, D., RUNNELS, L. AND STRUBING, C.
The TRP ion channel family.
NAT.REV.NEUROSCI. 2 6387-396 (2001). 
 
4. HARTENECK, C., PLANT, T. AND SCHULTZ, G.
From worm to man: three subfamilies of TRP channels.
TRENDS NEUROSCI. 23 159-166 (2000).
 
5. HOFMANN, T., OBUKHOV, A.G., SCHAEFER, M., HARTENECK, C., GUDERMANN, T. 
AND SCHULTZ, G. 
Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. 
NATURE 397 259-263 (1999).

Documentation
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response 
to a range of external and internal cell stimuli. Such Ca2+ entry is thought 
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other 
agents. In non-excitable cells, i.e. those that lack voltage-gated Ca2+
channels, such as hepatocytes, this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
 
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and the cDNA of a related protein, Trp-like, which show
similarity to voltage gated Ca2+ channels in the regions known as S3 through
S6, including the S5-S6 linker that forms the ion-selective channel pore [2].
This provided evidence that Trp and/or related proteins might form mammalian
capacitative Ca2+ entry channels. 
 
A number of Trp and Trp-like channel gene isoforms have now been cloned, 
including several mammalian homologues. The Trp family is thought to encode
at least 20 Ca2+-permeable channel proteins. Hydropathy analysis suggests 
that they share a common transmembrane (TM) topology. Each family member is 
predicted to possess 6 TM domains with intracellular N- and C-termini, which
is similar to the core structure of the pore-forming subunits of the voltage
-gated Na+ and Ca2+ channels. By analogy with these proteins, which have 
4 linked domains of 6 TM segments, it is likely that Trp channels are
homo- or heterotetramers of 4 single subunits [3].
 
The Trp family can be divided on the basis of sequence similarity into 3
subfamilies: short (S), long (L) and osm-like (O) Trp channels [4]. The
STrp subfamily includes Drosophila Trp and Trpl-like, and the mammalian
homologues TrpC1-7. Channels of the STrpC subfamily are activated following
receptor-mediated stimulation of different isoforms of phospholipase C [4].
 
TrpC3 was originally cloned from human tissue [1]; mouse and rat isoforms
have also been identified. Expression studies have shown that TrpC3 displays
low selectivity for Ca2+ over Na+, and, like TrpC6 and TrpC7, is activated
by diacylglycerol in a protein kinase C-independent manner [5].                                     
 
TRPCHANNEL3 is a 5-element fingerprint that provides a signature for
transient receptor potential channel 3. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved 
regions in the cytosolic termini, focusing on those sections that
characterise TrpC3 but distinguish it from other family members - motif 1 
lies in the intracellular N-terminal region; and motifs 2 to 5 reside within
the C-terminal cytoplasmic region. A single iteration on SPTR40_18f was 
required to reach convergence, no further sequences being identified beyond
the starting set.
Summary Information
3 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
533333
400000
300000
200000
12345
True Positives
Q13507        TRP3_MOUSE    TRP3_RAT      
Sequence Titles
Q13507      TRANSIENT RECEPTOR POTENTIAL RELATED CHANNEL 3 PROTEIN - HOMO SAPIENS (HUMAN). 
TRP3_MOUSE Short transient receptor potential channel 3 (TrpC3) (Transient receptor protein 3) (Mtrp3) (Receptor-activated cation channel TRP3) (trp-related protein 3) - Mus musculus (Mouse).
TRP3_RAT Short transient receptor potential channel 3 (TrpC3) - Rattus norvegicus (Rat).
Scan History
SPTR40_18f 1  150  NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
SAEPLERHGHKAS TRP3_MOUSE 281 281 -
SVEPLERHGHKAS TRP3_RAT 181 181 -
SAEPLEVHRHKAS Q13507 293 293 -

Motif 2 width=15
Element Seqn Id St Int Rpt
FVYFIMRITNFSKCR TRP3_MOUSE 704 410 -
FVYFIMRITNFSKCR TRP3_RAT 604 410 -
FVYFIMRIVNFPKCR Q13507 716 410 -

Motif 3 width=12
Element Seqn Id St Int Rpt
MGNSKSRLNLFT TRP3_MOUSE 730 11 -
MGNSKSRLNLFT TRP3_RAT 630 11 -
MGNSKSRLNLFT Q13507 742 11 -

Motif 4 width=17
Element Seqn Id St Int Rpt
NSRVFESHSFNSILNQP TRP3_MOUSE 744 2 -
NSRVFESHSFNSILNQP TRP3_RAT 644 2 -
NSRVFESHSFNSILNQP Q13507 756 2 -

Motif 5 width=22
Element Seqn Id St Int Rpt
EELAILIHKLSEKLNPSVLRCE TRP3_MOUSE 815 54 -
EELAILIHKLSEKLNPSALRCE TRP3_RAT 715 54 -
EELAILIHKLSEKLNPSMLRCE Q13507 827 54 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
SAEPLERHGHKAS TRP3_MOUSE 281 281 -
SVEPLERHGHKAS TRP3_RAT 181 181 -
SAEPLEVHRHKAS Q13507 293 293 -

Motif 2 width=15
Element Seqn Id St Int Rpt
FVYFIMRITNFSKCR TRP3_MOUSE 704 410 -
FVYFIMRITNFSKCR TRP3_RAT 604 410 -
FVYFIMRIVNFPKCR Q13507 716 410 -

Motif 3 width=12
Element Seqn Id St Int Rpt
MGNSKSRLNLFT TRP3_MOUSE 730 11 -
MGNSKSRLNLFT TRP3_RAT 630 11 -
MGNSKSRLNLFT Q13507 742 11 -

Motif 4 width=17
Element Seqn Id St Int Rpt
NSRVFESHSFNSILNQP TRP3_MOUSE 744 2 -
NSRVFESHSFNSILNQP TRP3_RAT 644 2 -
NSRVFESHSFNSILNQP Q13507 756 2 -

Motif 5 width=22
Element Seqn Id St Int Rpt
EELAILIHKLSEKLNPSVLRCE TRP3_MOUSE 815 54 -
EELAILIHKLSEKLNPSALRCE TRP3_RAT 715 54 -
EELAILIHKLSEKLNPSMLRCE Q13507 827 54 -