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PR01611

Identifier
LIMPII  [View Relations]  [View Alignment]  
Accession
PR01611
No. of Motifs
9
Creation Date
23-DEC-2001
Title
Lysosome membrane protein II (LIMP II) signature
Database References
PRINTS; PR01609 CD36FAMILY
MIM; 602257
Literature References
1. VEGA, M.A., SEGUI-REAL, B., GARCIA, J.A., CALES, C., RODRIGUEZ, F.,
VANDERKERCKHOVE, J. AND SANDOVAL, I.V.
Cloning, sequencing, and expression of a cDNA encoding rat LIMP II, a novel
74-kDa lysosomal membrane protein related to the surface adhesion protein
CD36.
J.BIOL.CHEM. 266 16818-16824 (1991). 
 
2. FUJITA, H., EZAKI, J., NOGUCHI, Y., KONO, A., HIMENO, M. AND KATO, K.
Isolation and sequencing of a cDNA clone encoding 85kDa sialoglycoprotein
in rat liver lysosomal membranes.
BIOCHEM.BIOPHYS.RES.COMMUN. 178 444-452 (1991). 
 
3. FUJITA, H., TAKATA, Y., KONO, A., TANAKA, Y., TAKAHASHI, T., HIMENO, M.
AND KATO, K.
Isolation and sequencing of a cDNA clone encoding the 85 kDa human lysosomal
sialoglycoprotein (hLGP85) in human metastatic pancreas islet tumor cells.
BIOCHEM.BIOPHYS.RES.COMMUN. 184 604-611 (1992). 

Documentation
Lysosome membrane protein II (LIMP II) is a 478-residue glycoprotein
expressed in the membrane of lysosomes and secretory granules with lysosomal
properties [1]. The N-terminal segment (residues ~4-26) constitute an 
uncleavable signal peptide [1]. LIMP II possesses an additional C-terminal
hydrophobic region that, together with the signal peptide, may anchor the
protein to the membrane [1]. The major portion of the protein resides on the
luminal side and contains 11 potential N-glycosylation sites and 5 cysteine
residues. The N- and C-terminal ends of the protein constitute short 
cytoplasmic tails. 
 
Comparison of the sequence to that of other lysosomal membrane glycoproteins
reveals no similarity. Glycine-tyrosine residues (the so-called GY motif),
believed to constitute an important signal for delivery of lysosomal 
membrane glycoproteins to lysosomes, are not contained in the cytoplasmic 
tail [2,3]. The protein thus appears to be a unique LIMP that does not 
require tyrosine residues for targeting to lysosomes [2]. Sequence database 
searches reveal extensive similarity between LIMP II and the cell surface 
protein CD36 involved in cell adhesion [1]. 
 
LIMPII is a 9-element fingerprint that provides a signature for lysosome
membrane protein LIMP II. The fingerprint was derived from an initial 
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning the full alignment length, focusing on those sections that
characterise LIMP II proteins but distinguish them from the rest of the
CD36 adhesion molecule family - motif 1 encodes the first TM domain; motifs
2-8 span the lumenal domain; and motif 9 encodes the C-terminal cytoplasmic
tail. Two iterations on SPTR40_18f were required to reach convergence, at
which point a true set comprising 3 sequences was identified. 
Summary Information
3 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9333333333
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
LYII_HUMAN    LYII_RAT      O35114        
Sequence Titles
LYII_HUMAN  Lysosome membrane protein II (LIMP II) (85 kDa lysosomal membrane sialoglycoprotein) (LGP85) (CD36 antigen-like 2) - Homo sapiens (Human). 
LYII_RAT Lysosome membrane protein II (LIMP II) (85 kDa lysosomal membrane sialoglycoprotein) (LGP85) (CD36 antigen-like 2) - Rattus norvegicus (Rat).
O35114 CD36 ANTIGEN (COLLAGEN TYPE I RECEPTOR, THROMBOSPONDIN RECEPTOR)-LIKE 2 (MLGP85/LIMP II) - Mus musculus (Mouse).
Scan History
SPTR40_18f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
RCCFYTAGTLSLLLLVTSVTLLVAR LYII_HUMAN 2 2 -
RCCFYTAGTLSLLLLVTSVTLLVAR LYII_RAT 2 2 -

Motif 2 width=24
Element Seqn Id St Int Rpt
FQKAVDQSIEKKIVLRNGTEAFDS LYII_HUMAN 28 1 -
FQKAVDQTIEKNMVLQNGTKVFDS LYII_RAT 28 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
DPKIDLIRTLNIPVLTVIE LYII_HUMAN 126 74 -
DPTVDLIRTINIPLLTVVE LYII_RAT 126 74 -

Motif 4 width=21
Element Seqn Id St Int Rpt
FLREIIEAMLKAYQQKLFVTH LYII_HUMAN 150 5 -
FLREIIEAMLKAYQQTLFVTH LYII_RAT 150 5 -

Motif 5 width=18
Element Seqn Id St Int Rpt
DEILSLIHVFRPDISPYF LYII_HUMAN 181 10 -
DEVLSLVHIFRPDVSPNF LYII_RAT 181 10 -

Motif 6 width=17
Element Seqn Id St Int Rpt
HPLITKDEVLYVFPSDF LYII_HUMAN 256 57 -
HPLISKDETLYIFPSDF LYII_RAT 256 57 -

Motif 7 width=21
Element Seqn Id St Int Rpt
PAEILANTSDNAGFCIPEGNC LYII_HUMAN 297 24 -
PAEILANSSENAGFCIPEGNC LYII_RAT 297 24 -

Motif 8 width=22
Element Seqn Id St Int Rpt
NIYVKKLDDFVETGDIRTMVFP LYII_HUMAN 385 67 -
NTYVKKLDDFVETGNIRTMVFP LYII_RAT 385 67 -

Motif 9 width=24
Element Seqn Id St Int Rpt
TWLACKGQGSMDEGTADERAPLIR LYII_HUMAN 453 46 -
TWLACRGQGSTDEGTADERAPLIR LYII_RAT 453 46 -
Final Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
RCCFYTAGTLSLLLLVTSVTLLVAR LYII_HUMAN 2 2 -
RCCFYTAGTLSLLLLVTSVTLLVAR LYII_RAT 2 2 -
RCCFYTAGTLSLLLLVTSVTLLVAR O35114 3 3 -

Motif 2 width=24
Element Seqn Id St Int Rpt
FQKAVDQSIEKKIVLRNGTEAFDS LYII_HUMAN 28 1 -
FQKAVDQTIEKNMVLQNGTKVFDS LYII_RAT 28 1 -
FQKAVDQTIEKNMVLQNGTKVFNS O35114 29 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
DPKIDLIRTLNIPVLTVIE LYII_HUMAN 126 74 -
DPTVDLIRTINIPLLTVVE LYII_RAT 126 74 -
DPNVDLIRTINIPLLTVVD O35114 127 74 -

Motif 4 width=21
Element Seqn Id St Int Rpt
FLREIIEAMLKAYQQKLFVTH LYII_HUMAN 150 5 -
FLREIIEAMLKAYQQTLFVTH LYII_RAT 150 5 -
LLRELIEAMLKAYQQKLFVIH O35114 151 5 -

Motif 5 width=18
Element Seqn Id St Int Rpt
DEILSLIHVFRPDISPYF LYII_HUMAN 181 10 -
DEVLSLVHIFRPDVSPNF LYII_RAT 181 10 -
DEILSLVHIFKPDVSPNF O35114 182 10 -

Motif 6 width=17
Element Seqn Id St Int Rpt
HPLITKDEVLYVFPSDF LYII_HUMAN 256 57 -
HPLISKDETLYIFPSDF LYII_RAT 256 57 -
HPLISKDEVLYLFPSDL O35114 257 57 -

Motif 7 width=21
Element Seqn Id St Int Rpt
PAEILANTSDNAGFCIPEGNC LYII_HUMAN 297 24 -
PAEILANSSENAGFCIPEGNC LYII_RAT 297 24 -
PAEILANTSENAGFCIPEGNC O35114 298 24 -

Motif 8 width=22
Element Seqn Id St Int Rpt
NIYVKKLDDFVETGDIRTMVFP LYII_HUMAN 385 67 -
NTYVKKLDDFVETGNIRTMVFP LYII_RAT 385 67 -
NTYVRKLDDFVETGDIRTMVFP O35114 386 67 -

Motif 9 width=24
Element Seqn Id St Int Rpt
TWLACKGQGSMDEGTADERAPLIR LYII_HUMAN 453 46 -
TWLACRGQGSTDEGTADERAPLIR LYII_RAT 453 46 -
TWLACRGQGSMDEGTADERAPLIR O35114 454 46 -