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PR01604

Identifier
KCNE1CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01604
No. of Motifs
4
Creation Date
12-SEP-2001
Title
KCNE voltage-gated K+ channel beta-1 subunit signature
Database References
PRINTS; PR00168 KCNECHANNEL
Literature References
1. MILLER, C.
An overview of the potassium channel family.
GENOME BIOL. 1(4) 1-5 (2000).
 
2. CONLEY, E.C. AND BRAMMAR, W.J.
MinK.
IN THE ION CHANNEL FACTSBOOK, VOLUME IV, ACADEMIC PRESS, 1999, PP.703-767.
 
3. PICCINI, M., VITELLI, F., SERI, M., GALIETTA, L.J.V., MORAN, O., BULFONE,
A., BANFI, S., POBER, B. AND RENIERI, A.
KCNE1-like gene is deleted in AMME contigious gene syndrome: identification
and characterization of the human and mouse homologs.
GENOMICS 60 251-257 (1999).

Documentation
Potassium ion (K+) channels are a structurally diverse group of proteins
that facilitate the flow of K+ ions across cell membranes. They are
ubiquitous, being present in virtually all cell types. Activation of K+
channels tends to hyperpolarise cells, reducing the membrane's electrical
resistance, dampening nervous activity. In eukaryotic cells, K+ channels
are involved in neural signalling and generation of the cardiac rhythm, and
act as effectors in signal transduction pathways involving G protein-
coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
maintenance of ionic homeostasis [1].
 
Some types of K+ channel are closed at the resting potential of the cell,
but open on membrane depolarisation, and are thus known as voltage-gated 
channels. Each of these types of channel typically comprises 4 pore-forming
alpha subunits that may associate with one of a number of different types of
beta subunit. Two types of beta subunit (KCNE and KCNAB) are presently known
to associate with voltage-gated alpha subunits (Kv, KCNQ and eag-like).
However, not all combinations of alpha and beta subunits are possible.
 
The KCNE family of K+ channel subunits are membrane glycoproteins that 
possess a single transmembrane (TM) domain. They share no structural 
relationship with the alpha subunit proteins, which possess pore forming 
domains. The subunits appear to have a regulatory function, modulating the 
kinetics and voltage dependence of the alpha subunits of voltage-dependent
K+ channels [2]. KCNE subunits are formed from short polypeptides of ~130
amino acids, and are divided into five subfamilies: KCNE1 (MinK/IsK),
KCNE2 (MiRP1), KCNE3 (MiRP2), KCNE4 (MiRP3) and KCNE1L (AMMECR2).
 
KCNE1 subunits associate with KCNQ1 alpha subunits to form channels that
are responsible for the IkS currents that determine the duration of the
action potential in cardiac muscle. Mutations in both of the genes encoding
these subunits cause an inherited disorder that increases the risk of death
from cardiac arrhythmia (long QT syndrome type 1) and Jervell and
Lange-Nielsen syndrome, associated with congenital deafness [3].
 
KCNE1CHANNEL is a 4-element fingerprint that provides a signature for the
KCNE voltage-gated K+ channel beta-1 subunit. The fingerprint was derived
from an initial alignment of 7 sequences: the motifs were drawn from
conserved regions spanning the N- and C-terminal portions of the alignment,
focusing on those sections that characterise the KCNE1 subunits but
distinguish them from the rest of the KCNE family - motifs 1 and 2 lie
in the extracellular N-terminus; and motifs 3 and 4 lie in the cytoplasmic
C-terminus. A single iteration on SPTR39.22_17.3f was required to reach
convergence, no further sequences being identified beyond the starting set.
Summary Information
7 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
47777
30000
20000
1234
True Positives
MINK_FELCA    MINK_HUMAN    MINK_MOUSE    MINK_PIG      
MINK_RAT Q28705 Q60409
Sequence Titles
MINK_FELCA  ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Felis silvestris catus (Cat). 
MINK_HUMAN ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Homo sapiens (Human).
MINK_MOUSE ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Mus musculus (Mouse).
MINK_PIG ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Sus scrofa (Pig).
MINK_RAT ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Rattus norvegicus (Rat).
Q28705 CARDIAC DELAYED RECTIFIER POTASSIUM CHANNEL PROTEIN - ORYCTOLAGUS CUNICULUS (RABBIT).
Q60409 POTASSIUM CHANNEL - CAVIA PORCELLUS (GUINEA PIG).
Scan History
SPTR39.22_17.3f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
VLPFLASLWQET MINK_RAT 9 9 -
VLPFLASLWQET MINK_PIG 9 9 -
VLPFLARLWQET MINK_MOUSE 9 9 -
VMPFLTTVWQGT Q60409 9 9 -
VTPFLTKLWQET MINK_HUMAN 9 9 -
VMPFLTTLGEET Q28705 9 9 -
TTPFLNALWQGT MINK_FELCA 9 9 -

Motif 2 width=13
Element Seqn Id St Int Rpt
LARRSQLRDDSKL MINK_RAT 31 10 -
LARRSQLRDDSKL MINK_PIG 31 10 -
LARKSQLRDDSKL MINK_MOUSE 30 9 -
LARRSPLRDDGKL Q60409 30 9 -
LARRSPRSSDGKL MINK_HUMAN 30 9 -
LARRGPLGDDGQM Q28705 31 10 -
LARRSPGGDDSQL MINK_FELCA 30 9 -

Motif 3 width=15
Element Seqn Id St Int Rpt
ALFQARVLESFRACY MINK_RAT 94 50 -
ALFQARVLESFRACY MINK_PIG 94 50 -
AVFQARVLESFRACY MINK_MOUSE 93 50 -
AFFQARVLENCRSCC Q60409 93 50 -
AYVQARVLESYRSCY MINK_HUMAN 93 50 -
AYFQARVLESCRGCY Q28705 94 50 -
AYLQARVLESYKACY MINK_FELCA 93 50 -

Motif 4 width=18
Element Seqn Id St Int Rpt
VIENQAAVEQPATHLPEL MINK_RAT 109 0 -
AIENQAAVEQPATHLPEL MINK_PIG 109 0 -
VIENQAAVEQPATHLPEL MINK_MOUSE 108 0 -
VIENQLTVEQPNTYLPEL Q60409 108 0 -
VVENHLAIEQPNTHLPET MINK_HUMAN 108 0 -
VLENQLAVEHPDTHLPEL Q28705 109 0 -
VIENQLAVERPNAHLPEI MINK_FELCA 108 0 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
VLPFLASLWQET MINK_RAT 9 9 -
VLPFLASLWQET MINK_PIG 9 9 -
VLPFLARLWQET MINK_MOUSE 9 9 -
VMPFLTTVWQGT Q60409 9 9 -
VTPFLTKLWQET MINK_HUMAN 9 9 -
VMPFLTTLGEET Q28705 9 9 -
TTPFLNALWQGT MINK_FELCA 9 9 -

Motif 2 width=13
Element Seqn Id St Int Rpt
LARRSQLRDDSKL MINK_RAT 31 10 -
LARRSQLRDDSKL MINK_PIG 31 10 -
LARKSQLRDDSKL MINK_MOUSE 30 9 -
LARRSPLRDDGKL Q60409 30 9 -
LARRSPRSSDGKL MINK_HUMAN 30 9 -
LARRGPLGDDGQM Q28705 31 10 -
LARRSPGGDDSQL MINK_FELCA 30 9 -

Motif 3 width=15
Element Seqn Id St Int Rpt
ALFQARVLESFRACY MINK_RAT 94 50 -
ALFQARVLESFRACY MINK_PIG 94 50 -
AVFQARVLESFRACY MINK_MOUSE 93 50 -
AFFQARVLENCRSCC Q60409 93 50 -
AYVQARVLESYRSCY MINK_HUMAN 93 50 -
AYFQARVLESCRGCY Q28705 94 50 -
AYLQARVLESYKACY MINK_FELCA 93 50 -

Motif 4 width=18
Element Seqn Id St Int Rpt
VIENQAAVEQPATHLPEL MINK_RAT 109 0 -
AIENQAAVEQPATHLPEL MINK_PIG 109 0 -
VIENQAAVEQPATHLPEL MINK_MOUSE 108 0 -
VIENQLTVEQPNTYLPEL Q60409 108 0 -
VVENHLAIEQPNTHLPET MINK_HUMAN 108 0 -
VLENQLAVEHPDTHLPEL Q28705 109 0 -
VIENQLAVERPNAHLPEI MINK_FELCA 108 0 -