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PR01603

Identifier
VDCCGAMMA4  [View Relations]  [View Alignment]  
Accession
PR01603
No. of Motifs
5
Creation Date
07-SEP-2001
Title
Voltage-dependent calcium channel gamma-4 subunit signature
Database References
PRINTS; PR01792 VDCCGAMMA
Literature References
1. BITO, H., DEISSEROTH, K. AND TSIEN, R.
Ca2+-dependent regulation in neuronal gene expression. 
CURR.OPIN.NEUROBIOL. 7 419-429 (1997).
 
2. DUNLAP, K., LEUBKE, J. AND TURNER, T.
Exocytotic calcium channels in mammalian central neurons. 
TRENDS NEUROSCI. 18 89-98 (1995). 
 
3. AHLIJANIAN, M., WESTENBROEK, R. AND CATTERALL, W.
Subunit structure and localization of dihydropyridine-sensitive calcium 
channels in mammalian brain, spinal cord, and retina.
NEURON 4 819-832 (1990).
 
4. WITCHER, D., DE WAARD, M., SAKAMOTO, J., FRANZINI-ARMSTRONG, C., 
PRAGNELL, M., KAHL, S. AND CAMPBELL, K.
Subunit identification and reconstitution of the N-type Ca2+ channel complex
purified from brain.
SCIENCE 261 486-489 (1993).
 
5. BURGESS, D., GEFRIDES, L., FOREMAN, P. AND NOEBELS, J.
A cluster of three novel Ca2+ channel gamma subunit genes on chromosome 
19q13.4: evolution and expression profile of the gamma subunit gene family.
GENOMICS 71 339-350 (2001).
 
6. KLUGBAUER, N., DAI, S., SPECHT, V., LACINOVA, L., MARAIS, E., BOHN, G. 
AND HOFMANN, F.
A family of gamma-like calcium channel subunits.
FEBS LETT. 470 189-197 (2000).
 
7. ROUSSET, M., CENS, T., RESTITUITO, S., BARRERE, C., BLACK, J., MCENERY,
M. AND CHARNET, P.
Functional roles of gamma2, gamma3 and gamma4, three new Ca2+ channel 
subunits, in P/Q-type Ca2+ channel expressed in Xenopus oocytes.
J.PHYSIOL. 532 583-593 (2001).

Documentation
Voltage-dependent calcium channels are a diverse family of proteins that 
encompass a variety of biological functions, including presynaptic
neurotransmitter release and protein signalling within the cell [1,2]. The
high voltage-activated channels (L-, N-, P-, Q- and R-type channels)
comprise the alpha-1 subunit, which creates the pore for the import of 
extracellular calcium ions [2]. The activity of this pore is modulated by 
four tightly-coupled subunits: an intracellular beta subunit; a
transmembrane (TM) gamma subunit; and a disulphide-linked complex of alpha-2
and delta subunits, which are proteolytically cleaved from the same gene 
product [3,4].
 
The voltage-dependent calcium channel gamma (VDCCG) subunit family consists 
of at least 8 members, which share a number of common structural features 
[5]. Each member is predicted to possess four TM domains, with intracellular
N- and C- termini. The first extracellular loop contains a highly conserved
N-glycosylation site and a pair of conserved cysteine residues. The 
C-terminal seven residues of VDCCG-2, -3, -4 and -8 are also conserved and 
contain a consensus site for phosphorylation by cAMP and cGMP-dependent
protein kinases, and a target site for binding by PDZ domain proteins [5].
 
The VDCCG-4 subunit is predominantly expressed in neuronal tissue, although
there is some evidence for expression in lung and prostate [5,6]. The
modulatory properties of the subunit have been investigated using
heterologous expression systems. Coexpression of the VDCGG-4 subunit with 
P/Q-type channels shifts the steady-state inactivation curve of these 
channels to more hyperpolarised potentials [6,7].
 
VDCCGAMMA4 is a 5-element fingerprint that provides a signature for the
voltage-dependent calcium channel gamma-4 subunit proteins. The fingerprint
was derived from an initial alignment of 2 sequences: the motifs were drawn
from conserved regions spanning virtually the full alignment length, 
focusing on those sections that characterise the VDCCG-4 sunbunits but 
distinguish them from other family members - motif 1 lies in the first 
extracellular loop; motif two spans part of TM domain 2 and extends into 
the intracellular loop; and motifs 3, 4 and 5 lie in the C-terminal 
cytoplasmic region. A single iteration on SPTR39.22_17.3f was required to 
reach convergence, no further sequences being identified beyond the 
starting set.
Summary Information
2 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
522222
400000
300000
200000
12345
True Positives
CCG4_HUMAN    CCG4_MOUSE    
Sequence Titles
CCG4_HUMAN  Voltage-dependent calcium channel gamma-4 subunit (Neuronal voltage- gated calcium channel gamma-4 subunit) - Homo sapiens (Human). 
CCG4_MOUSE Voltage-dependent calcium channel gamma-4 subunit (Neuronal voltage- gated calcium channel gamma-4 subunit) - Mus musculus (Mouse).
Scan History
SPTR39.22_17.3f 1  150  NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
SSAHICNGTNLTMDD CCG4_HUMAN 36 36 -
SSAHICNGTNLTMDD CCG4_MOUSE 36 36 -

Motif 2 width=11
Element Seqn Id St Int Rpt
IGAGRIYSRKN CCG4_HUMAN 125 74 -
IGAGRIYSRKN CCG4_MOUSE 125 74 -

Motif 3 width=12
Element Seqn Id St Int Rpt
KASSSSPYARMP CCG4_HUMAN 223 87 -
KASSSSPYARMP CCG4_MOUSE 223 87 -

Motif 4 width=11
Element Seqn Id St Int Rpt
KITGAIPMGEL CCG4_HUMAN 264 29 -
KITGAIPMGEL CCG4_MOUSE 264 29 -

Motif 5 width=13
Element Seqn Id St Int Rpt
TTAASYSPDQEAS CCG4_HUMAN 287 12 -
TTAASYSPDQDAG CCG4_MOUSE 287 12 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
SSAHICNGTNLTMDD CCG4_HUMAN 36 36 -
SSAHICNGTNLTMDD CCG4_MOUSE 36 36 -

Motif 2 width=11
Element Seqn Id St Int Rpt
IGAGRIYSRKN CCG4_HUMAN 125 74 -
IGAGRIYSRKN CCG4_MOUSE 125 74 -

Motif 3 width=12
Element Seqn Id St Int Rpt
KASSSSPYARMP CCG4_HUMAN 223 87 -
KASSSSPYARMP CCG4_MOUSE 223 87 -

Motif 4 width=11
Element Seqn Id St Int Rpt
KITGAIPMGEL CCG4_HUMAN 264 29 -
KITGAIPMGEL CCG4_MOUSE 264 29 -

Motif 5 width=13
Element Seqn Id St Int Rpt
TTAASYSPDQEAS CCG4_HUMAN 287 12 -
TTAASYSPDQDAG CCG4_MOUSE 287 12 -