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PR01601

Identifier
VDCCGAMMA1  [View Relations]  [View Alignment]  
Accession
PR01601
No. of Motifs
3
Creation Date
07-SEP-2001
Title
Voltage-dependent calcium channel gamma-1 subunit signature
Database References
PRINTS; PR01792 VDCCGAMMA
PRODOM; PD016829
MIM; 114209
Literature References
1. BITO, H., DEISSEROTH, K. AND TSIEN, R.
Ca2+-dependent regulation in neuronal gene expression. 
CURR.OPIN.NEUROBIOL. 7 419-429 (1997).
 
2. DUNLAP, K., LEUBKE, J. AND TURNER, T.
Exocytotic calcium channels in mammalian central neurons. 
TRENDS NEUROSCI. 18 89-98 (1995). 
 
3. AHLIJANIAN, M., WESTENBROEK, R. AND CATTERALL, W.
Subunit structure and localization of dihydropyridine-sensitive calcium 
channels in mammalian brain, spinal cord, and retina.
NEURON 4 819-832 (1990).
 
4. WITCHER, D., DE WAARD, M., SAKAMOTO, J., FRANZINI-ARMSTRONG, C., 
PRAGNELL, M., KAHL, S. AND CAMPBELL, K.
Subunit identification and reconstitution of the N-type Ca2+ channel complex
purified from brain.
SCIENCE 261 486-489 (1993).
 
5. BURGESS, D., GEFRIDES, L., FOREMAN, P. AND NOEBELS, J.
A cluster of three novel Ca2+ channel gamma subunit genes on chromosome 
19q13.4: evolution and expression profile of the gamma subunit gene family.
GENOMICS 71 339-350 (2001).
 
6. JAY, S., ELLIS, S., MCCUE, A., WILLIAMS, M., VEDVICK, T., HARPOLD, M. AND
CAMPBELL, K.
Primary structure of the gamma subunit of the DHP-sensitive calcium channel
from skeletal muscle.
SCIENCE 248 490-492 (1990).
 
7. WEI, X., PEREZ-REYES, E., LACERDA, A., SCHUSTER, G., BROWN, A. AND 
BIRNBAUMER, L.
Heterologous regulation of the cardiac Ca2+ channel alpha 1 subunit by 
skeletal muscle beta and gamma subunits. Implications for the structure of 
cardiac L-type Ca2+ channels.
J.BIOL.CHEM. 266 21943-21947 (1991).
 
8. SINGER, D., BIEL, M., LOTAN, I., FLOCKERZI, V, HOFMANN, F. AND DASCAL, N.
The roles of the subunits in the function of the calcium channel.
SCIENCE 253 1553-1557 (1991).

Documentation
Voltage-dependent calcium channels are a diverse family of proteins that 
encompass a variety of biological functions, including presynaptic
neurotransmitter release and protein signalling within the cell [1,2]. The
high voltage-activated channels (L-, N-, P-, Q- and R-type channels)
comprise the alpha-1 subunit, which creates the pore for the import of 
extracellular calcium ions [2]. The activity of this pore is modulated by 
four tightly-coupled subunits: an intracellular beta subunit; a
transmembrane (TM) gamma subunit; and a disulphide-linked complex of alpha-2
and delta subunits, which are proteolytically cleaved from the same gene 
product [3,4].
 
The voltage-dependent calcium channel gamma (VDCCG) subunit family consists 
of at least 8 members, which share a number of common structural features 
[5]. Each member is predicted to possess four TM domains, with intracellular
N- and C- termini. The first extracellular loop contains a highly conserved
N-glycosylation site and a pair of conserved cysteine residues. The 
C-terminal seven residues of VDCCG-2, -3, -4 and -8 are also conserved and 
contain a consensus site for phosphorylation by cAMP and cGMP-dependent
protein kinases, and a target site for binding by PDZ domain proteins [5].
 
The VDCCG-1 subunit is a 25kDa protein expressed exclusively in skeletal 
muscle cells, where it functions as a dihydropyridine-sensitive, L-type 
calcium channel subunit [6]. The modulatory properties of VDCCG-1 subunits 
have been investigated using heterologous expression systems. Coexpression
of VDCCG-1 subunits with L-type or P/Q-type channels induces moderate 
changes in activation and inactivation properties, and modification of the
peak current amplitude of these channels [7,8].
 
VDCCGAMMA1 is a 3-element fingerprint that provides a signature for the
voltage-dependent calcium channel gamma-1 subunit proteins. The fingerprint
was derived from an initial alignment of 2 sequences: the motifs were drawn
from conserved regions spanning virtually the full alignment length,
focusing on those sections that characterise the VDCCG-1 subunits but 
distinguish them from other family members - motif 1 lies in the first 
extracellular loop; motif 2 spans part of the second TM domain, the
intracellular loop and part of the third TM domain; and motif 3 encodes
part of the fourth TM domain and the cytoplasmic C-terminal region. Two
iterations on SPTR39.22_17.3f were required to reach convergence, at which 
point a true set comprising 4 sequences was identified. 
Summary Information
4 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3444
2000
123
True Positives
CCG1_HUMAN    CCG1_MOUSE    CCG1_RABIT    CCG1_RAT      
Sequence Titles
CCG1_HUMAN  Voltage-dependent calcium channel gamma-1 subunit (Dihydropyridine- sensitive L-type, skeletal muscle calcium channel gamma subunit) - Homo sapiens (Human). 
CCG1_MOUSE Voltage-dependent calcium channel gamma-1 subunit (Dihydropyridine- sensitive L-type, skeletal muscle calcium channel gamma subunit) - Mus musculus (Mouse).
CCG1_RABIT Voltage-dependent calcium channel gamma-1 subunit (Dihydropyridine- sensitive L-type, skeletal muscle calcium channel gamma subunit) - Oryctolagus cuniculus (Rabbit).
CCG1_RAT Voltage-dependent calcium channel gamma-1 subunit (Dihydropyridine- sensitive L-type, skeletal muscle calcium channel gamma subunit) - Rattus norvegicus (Rat).
Scan History
SPTR39.22_17.3f 2  150  NSINGLE    
Initial Motifs
Motif 1  width=27
Element Seqn Id St Int Rpt
TDHWAVLSPHMEHHNTTCEAAHFGLWR CCG1_HUMAN 29 29 -
SDHWAVLSPHMENHNTTCEAAHFGLWR CCG1_RABIT 29 29 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LLSLGKKRDYLLRPAS CCG1_HUMAN 126 70 -
LMAFRKKRDYLLRPAS CCG1_RABIT 126 70 -

Motif 3 width=24
Element Seqn Id St Int Rpt
LLLFSLPRMPRNPWESCMDAEPEH CCG1_HUMAN 199 57 -
LLLFSLPRMPQNPWESCMDAEPEH CCG1_RABIT 199 57 -
Final Motifs
Motif 1  width=27
Element Seqn Id St Int Rpt
TDHWAVLSPHMEHHNTTCEAAHFGLWR CCG1_HUMAN 29 29 -
SDHWAVLSPHMENHNTTCEAAHFGLWR CCG1_RABIT 29 29 -
TDHWAVLSPHLEHHNETCEAAHFGLWR CCG1_MOUSE 29 29 -
TDHWAVLSPHLEHHNETCVAAHFGLWR CCG1_RAT 29 29 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LLSLGKKRDYLLRPAS CCG1_HUMAN 126 70 -
LMAFRKKRDYLLRPAS CCG1_RABIT 126 70 -
FLSFGNKRDYLLRPAS CCG1_MOUSE 127 71 -
FLSFGNKRDYLLRPAS CCG1_RAT 127 71 -

Motif 3 width=24
Element Seqn Id St Int Rpt
LLLFSLPRMPRNPWESCMDAEPEH CCG1_HUMAN 199 57 -
LLLFSLPRMPQNPWESCMDAEPEH CCG1_RABIT 199 57 -
LLLFSLPRMPQNPWESCMDAEPEH CCG1_MOUSE 200 57 -
LLLFSLPRMPQNPWESCMDTESEH CCG1_RAT 200 57 -