Identifier | FFH4HYDRLASE  [View Relations]  [View Alignment]  
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Accession | PR01575 |
No. of Motifs | 6 |
Creation Date | 19-SEP-2001 |
Title | Formyltetrahydrofolate deformylase signature |
Database References | PDB; 1GRC SCOP; 1GRC CATH; 1GRC
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Literature References | 1. NAGY, P.L., MCCORKLE, G.M. AND ZALKIN, H.
purU, a source of formate for purT-dependent phosphoribosyl-N-formyl-
glycinamide synthesis.
J.BACTERIOL. 175 7066-7073 (1993).
2. NAGY, P.L., MAROLEWSKI, A., BENKOVIC, S.J. AND ZALKIN, H.
Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to
balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts
in Escherichia coli.
J.BACTERIOL. 177 1292-1298 (1995).
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Documentation | An Escherichia coli gene designated purU has been identified and
characterised [1]. The gene codes for a 280-amino-acid protein, PurU, whose
C-terminal segment (residues 84-280) exhibits 27% identity with 5'-
phosphoribosylglycinamide (GAR) transformylase, the product of purN [1].
PurU is an enzyme that catalyses the hydrolysis of 10-formyltetrahydrofolate
(formyl-FH4) to FH4 and formate [2]:
10-FORMYLTETRAHYDROFOLATE + H(2)O = FORMATE + TETRAHYDROFOLATE
Formyl-FH4 hydrolase generates the formate that is used by purT-encoded
5'-phosphoribosylglycinamide transformylase for step three of de novo purine
nucleotide synthesis [2]. Formyl-FH4 hydrolase, a hexamer of 32kDa subunits,
is activated by methionine and inhibited by glycine [2]. Heterotropic
cooperativity is observed for activation by methionine in the presence of
glycine and for inhibition by glycine in the presence of methionine [2].
These results suggest that formyl-FH4 hydrolase is a regulatory enzyme whose
main function is to balance the pools of FH4 and C1-FH4 in response to
changing growth conditions [2]. The enzyme uses methionine and glycine to
sense the pools of C1-FH4 and FH4, respectively [2].
FFH4HYDRLASE is a 6-element fingerprint that provides a signature for
formyltetrahydrofolate deformylase. The fingerprint was derived from an
initial alignment of 6 sequences: the motifs were drawn from conserved
regions spanning the full alignment length. Six iterations on
SPTR39.22_17.3f were required to reach convergence, at which point a true
set comprising 18 sequences was identified. Several partial matches were
also found, all of which are formyltetrahydrofolate deformylases and puru-
like proteins that fail to make significant matches with one or more motifs.
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Summary Information | 18 codes involving 6 elements 3 codes involving 5 elements 1 codes involving 4 elements 1 codes involving 3 elements 1 codes involving 2 elements
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Composite Feature Index | 6 | 18 | 18 | 18 | 18 | 18 | 18 | 5 | 2 | 1 | 3 | 3 | 3 | 3 | 4 | 1 | 1 | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | | 1 | 2 | 3 | 4 | 5 | 6 |
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True Positives | O25975 O34990 O67681 PURU_CORSP PURU_ECOLI PURU_HAEIN PURU_MYCTU PURU_SHIFL PURU_SYNY3 Q9A2D5 Q9CMF6 Q9HW87 Q9K7U4 Q9KQK6 Q9PCF0 Q9RWT1 Q9X7F7 Q9ZJI2 |
True Positive Partials | |
Sequence Titles | O25975 FORMYLTETRAHYDROFOLATE HYDROLASE (PURU) - Helicobacter pylori (Campylobacter pylori). O34990 YKKE - Bacillus subtilis. O67681 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Aquifex aeolicus. PURU_CORSP FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) (FORMYL-FH(4) HYDROLASE) - Corynebacterium sp. (strain P-1). PURU_ECOLI FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) (FORMYL-FH(4) HYDROLASE) - Escherichia coli. PURU_HAEIN FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) (FORMYL-FH(4) HYDROLASE) - Haemophilus influenzae. PURU_MYCTU FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) (FORMYL-FH(4) HYDROLASE) - Mycobacterium tuberculosis. PURU_SHIFL FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) (FORMYL-FH(4) HYDROLASE) - Shigella flexneri. PURU_SYNY3 FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) (FORMYL-FH(4) HYDROLASE) - Synechocystis sp. (strain PCC 6803). Q9A2D5 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Caulobacter crescentus. Q9CMF6 PURU - Pasteurella multocida. Q9HW87 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Pseudomonas aeruginosa. Q9K7U4 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Bacillus halodurans. Q9KQK6 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Vibrio cholerae. Q9PCF0 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Xylella fastidiosa. Q9RWT1 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Deinococcus radiodurans. Q9X7F7 PURU PROTEIN - Methylobacterium sp. (strain CM4). Q9ZJI2 FORMYLTETRAHYDROFOLATE HYDROLASE - Helicobacter pylori J99 (Campylobacter pylori J99). Q9AGP9 PUTATIVE FORMYLTETRAHYDROFOLATE DEFORMYLASE - Arthrobacter globiformis. Q9HTE4 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Pseudomonas aeruginosa. Q9PPC9 FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) - Campylobacter jejuni. O06440 PURU-LIKE PROTEIN - Rhodobacter capsulatus (Rhodopseudomonas capsulata). O23579 FORMYLTRANSFERASE PURU HOMOLOG - Arabidopsis thaliana (Mouse-ear cress). Q9HNU0 FORMYLTETRAHYDROFOLATE DEFORMYLASE - Halobacterium sp. (strain NRC-1).
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Scan History | SPTR39.22_17.3f 6 100 NSINGLE
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Initial Motifs | Motif 1 width=27 Element Seqn Id St Int Rpt LRTICPDQKGLIARITNICYKHELNIV PURU_SHIFL 9 9 - LRTICPDQKGLIARITNICYKHELNIV PURU_ECOLI 9 9 - LLTDCPDDKGLIAKITNICYKHQLNIL PURU_HAEIN 7 7 - LLVSCPDQPGIVAQIAQFIYQNQGNII PURU_SYNY3 8 8 - LLLRCHDRPGIIAAVSTFLARAGANII PURU_MYCTU 33 33 - LTLQCPEGIGIVHAVTGFLVRHQRTIV PURU_CORSP 9 9 - Motif 2 width=13 Element Seqn Id St Int Rpt VDHLTGRFFMRTE PURU_SHIFL 41 5 - VDHRTGRFFMRTE PURU_ECOLI 41 5 - VDFETKHFFMRTE PURU_HAEIN 39 5 - TDFSSGLFLNRVE PURU_SYNY3 40 5 - TAPEGGTFLQRAI PURU_MYCTU 66 6 - DDMSAGRLFLRVD PURU_CORSP 41 5 - Motif 3 width=23 Element Seqn Id St Int Rpt RIVILVTKEAHCLGDLLMKANYG PURU_SHIFL 86 32 - RIVILVTKEAHCLGDLLMKANYG PURU_ECOLI 86 32 - RIVILVTKEAHCLGDILMKNYYG PURU_HAEIN 84 32 - RLALWVSKQDHCLLDILWRWRSG PURU_SYNY3 90 37 - RVAIMASTEDHCLLDLLWRNRRG PURU_MYCTU 117 38 - KVLIMVSKFEHCLQDLLFRMHSG PURU_CORSP 91 37 - Motif 4 width=28 Element Seqn Id St Int Rpt GGLDVEIAAVIGNHDTLRSLVERFDIPF PURU_SHIFL 108 -1 - GGLDVEIAAVIGNHDTLRSLVERFDIPF PURU_ECOLI 108 -1 - GALDVEIAAVIGNHDNLRELVERFNIPF PURU_HAEIN 106 -1 - GELRCEIPLIISNHPDLKSIADQFGIDF PURU_SYNY3 112 -1 - GELEMSVVMVIANHPDLAAHVRPFGVPF PURU_MYCTU 139 -1 - GDLPIEVVGVASNHPDHRSLVEWYGIGF PURU_CORSP 113 -1 - Motif 5 width=23 Element Seqn Id St Int Rpt QDVIHVDHTYTAEDMMRAGRDVE PURU_SHIFL 232 96 - QDVIHVDHTYTAEDMMRAGRDVE PURU_ECOLI 232 96 - QNVINVDHTYNAEAMMRAGRDVE PURU_HAEIN 230 96 - QDVVRVSHRDNVDDLIRKGRDLE PURU_SYNY3 235 95 - QDVVRVDHTHTVDDLVRVGADVE PURU_MYCTU 262 95 - QQVVEVDHTYGPQDLVAAGRDSE PURU_CORSP 237 96 - Motif 6 width=26 Element Seqn Id St Int Rpt EKNVLSRALYKVLAQRVFVYGNRTII PURU_SHIFL 254 -1 - EKNVLSRALYKVLAQRVFVYGNRTII PURU_ECOLI 254 -1 - EKTVLSRALDLALHDRIFVYKNKTVV PURU_HAEIN 252 -1 - ERVVLARAVRLHLQHRILVYDNRTVV PURU_SYNY3 257 -1 - ERAVLSRAVLWHCQDRVIVHHNQTIV PURU_MYCTU 284 -1 - ECKALSNAVRWHCEGRVFLYGNRTVV PURU_CORSP 259 -1 -
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Final Motifs | Motif 1 width=27 Element Seqn Id St Int Rpt LRTICPDQKGLIARITNICYKHELNIV PURU_SHIFL 9 9 - LRTICPDQKGLIARITNICYKHELNIV PURU_ECOLI 9 9 - LLTDCPDDKGLIAKITNICYKHQLNII Q9CMF6 7 7 - LLTDCPDDKGLIAKITNICYKHQLNIL PURU_HAEIN 7 7 - LLISCHDQPGIVAAISTFLNHHGANIV Q9K7U4 11 11 - LVIACPDGVGIVAKVSNFLATYNGWIT Q9HW87 6 6 - LLTHCPDAPGLISKITNICYKHQLNII Q9KQK6 6 6 - LTISCADQPGIVAAVSQFLHNHGANII Q9RWT1 20 20 - LLVSCPDQPGIVSAVSAFLFEHGANII O34990 22 22 - LKIQAKDSKGLVSAISATIANKGYNIV Q9ZJI2 6 6 - LKIQARDSKGLVSTISTTIANKGYNIV O25975 6 6 - LLVSCPDQPGIVAQIAQFIYQNQGNII PURU_SYNY3 8 8 - LTLSCPDQRGIVAKVSAFLFERGCNIL Q9A2D5 3 3 - LLVSCPDRKGLVKEISSFIADNGGNIV O67681 6 6 - LLLRCHDRPGIIAAVSTFLARAGANII PURU_MYCTU 33 33 - LKISCADRPGIVAAVSGALLERDCNIV Q9X7F7 8 8 - LTLQCPEGIGIVHAVTGFLVRHQRTIV PURU_CORSP 9 9 - LTLSCPDCTGLVYRISGELFRAGCNIL Q9PCF0 7 7 - Motif 2 width=13 Element Seqn Id St Int Rpt VDHLTGRFFMRTE PURU_SHIFL 41 5 - VDHRTGRFFMRTE PURU_ECOLI 41 5 - VDFDTKHFFMRTE Q9CMF6 39 5 - VDFETKHFFMRTE PURU_HAEIN 39 5 - TDPEGGRFFMRVE Q9K7U4 44 6 - SDNDNGWFFMRHE Q9HW87 38 5 - VDNASGHFFMRTE Q9KQK6 38 5 - TDPAGGQFFMRME Q9RWT1 53 6 - TDPEGGRFFLRIE O34990 55 6 - VDPLKQRFFMRLK Q9ZJI2 38 5 - VDPLKQRFFMRLK O25975 38 5 - TDFSSGLFLNRVE PURU_SYNY3 40 5 - DDQETGQFFMRVV Q9A2D5 35 5 - IDEQTKTFLARVE O67681 38 5 - TAPEGGTFLQRAI PURU_MYCTU 66 6 - EDVIEKRFFMRVV Q9X7F7 40 5 - DDMSAGRLFLRVD PURU_CORSP 41 5 - GDKESHLFFLRVH Q9PCF0 39 5 - Motif 3 width=23 Element Seqn Id St Int Rpt RIVILVTKEAHCLGDLLMKANYG PURU_SHIFL 86 32 - RIVILVTKEAHCLGDLLMKANYG PURU_ECOLI 86 32 - RIVILVTKEAHCLGDILMKNYYG Q9CMF6 84 32 - RIVILVTKEAHCLGDILMKNYYG PURU_HAEIN 84 32 - RMAIFVSKEDHCLLELLWKWHSN Q9K7U4 94 37 - RVVLMASKESHCLADLLHRWHSG Q9HW87 88 37 - RIVILVTKEAHCLGDILMKNYDG Q9KQK6 83 32 - KMAVLVSRYDHCFLDLLWRRRRG Q9RWT1 104 38 - RVAIFVSKNLHCLHELIWEWQTG O34990 105 37 - NIILLATKESHCLGDLLLRVYGG Q9ZJI2 95 44 - NIILLATKESHCLGDLLLRVYGG O25975 95 44 - RLALWVSKQDHCLLDILWRWRSG PURU_SYNY3 90 37 - RVLLLASKFDHCLADLVYRWRIG Q9A2D5 83 35 - KVAIFVSKQEHCFYDLMHRFYSG O67681 88 37 - RVAIMASTEDHCLLDLLWRNRRG PURU_MYCTU 117 38 - RVMILVSRFDHCLVDILYRKRIG Q9X7F7 90 37 - KVLIMVSKFEHCLQDLLFRMHSG PURU_CORSP 91 37 - RLLVMVSKQGHCLNDLLFRIHSR Q9PCF0 88 36 - Motif 4 width=28 Element Seqn Id St Int Rpt GGLDVEIAAVIGNHDTLRSLVERFDIPF PURU_SHIFL 108 -1 - GGLDVEIAAVIGNHDTLRSLVERFDIPF PURU_ECOLI 108 -1 - GGLDVEIAAVIGNHDTLRTLVERFDIPF Q9CMF6 106 -1 - GALDVEIAAVIGNHDNLRELVERFNIPF PURU_HAEIN 106 -1 - NELICDIPLVISNHDELRDVVEGYGIPY Q9K7U4 116 -1 - GELDCEIPCVIANHDDLRSMVEWHGIPY Q9HW87 110 -1 - GSLDVDIAAVVGNYDTLQRLTERFDIPY Q9KQK6 105 -1 - GELNVEIPLILSNHEDLRRDAEMFGIPF Q9RWT1 126 -1 - GNLMAEIAVVISNHEEARELVERLNIPF O34990 127 -1 - GELNAQILGVISNHEILRPLVEKFDIPY Q9ZJI2 117 -1 - GELNAQILGVISNHEILRPLVEKFDIPY O25975 117 -1 - GELRCEIPLIISNHPDLKSIADQFGIDF PURU_SYNY3 112 -1 - GELPMDITGVVSNHPAQTYAHVDLSGLD Q9A2D5 105 -1 - GELKGEVKLVISNHEKARKTAEFFGVPF O67681 110 -1 - GELEMSVVMVIANHPDLAAHVRPFGVPF PURU_MYCTU 139 -1 - GELPMDLTAVVTNHAAENYAHLDLCGAP Q9X7F7 112 -1 - GDLPIEVVGVASNHPDHRSLVEWYGIGF PURU_CORSP 113 -1 - RQLQAKIVTVVSNHNDFAPLTASYGVPF Q9PCF0 110 -1 - Motif 5 width=23 Element Seqn Id St Int Rpt QDVIHVDHTYTAEDMMRAGRDVE PURU_SHIFL 232 96 - QDVIHVDHTYTAEDMMRAGRDVE PURU_ECOLI 232 96 - QNVINVDHTYNAEAMMRAGRDVE Q9CMF6 230 96 - QNVINVDHTYNAEAMMRAGRDVE PURU_HAEIN 230 96 - QDVLRVNHRYSVPQLRVAGRNVE Q9K7U4 240 96 - QDVVRVTHRDNVEDMVRLGKDVE Q9HW87 234 96 - QDVIPVDHTFSAQDMAQAGRDVE Q9KQK6 229 96 - QDVIPVTHRETPDTLMRMGRDVE Q9RWT1 250 96 - QDIKRVDHRDNAETLKNIGRTIE O34990 251 96 - QDTLPINHNYSVEKMRLAGKDIE Q9ZJI2 245 100 - QDTLPINHNYSVEKMRLAGKDIE O25975 245 100 - QDVVRVSHRDNVDDLIRKGRDLE PURU_SYNY3 235 95 - QDVERISHRDTPEDLVRKGRDIE Q9A2D5 230 97 - QDVVRVSHKDSLEDFIRKGKDIE O67681 234 96 - QDVVRVDHTHTVDDLVRVGADVE PURU_MYCTU 262 95 - QDVERISHSDSPEDLVRKGRDIE Q9X7F7 237 97 - QQVVEVDHTYGPQDLVAAGRDSE PURU_CORSP 237 96 - QDVARVDHSMTAHDLVRIGSDIE Q9PCF0 234 96 - Motif 6 width=26 Element Seqn Id St Int Rpt EKNVLSRALYKVLAQRVFVYGNRTII PURU_SHIFL 254 -1 - EKNVLSRALYKVLAQRVFVYGNRTII PURU_ECOLI 254 -1 - EKTVLSRALDLALHDRIFVYKNKTVV Q9CMF6 252 -1 - EKTVLSRALDLALHDRIFVYKNKTVV PURU_HAEIN 252 -1 - ERVVLARAVNWYLEDKIIVYSNKTVV Q9K7U4 262 -1 - EKLVLARGLRYHLEDRVLVHGNKTVV Q9HW87 256 -1 - EKNVLSKALNKVLNDHVFVYGNKTVI Q9KQK6 251 -1 - ERQVLARAVKAHVEDRVLVYGNKTVV Q9RWT1 272 -1 - ERSVLARAVKWHLEDRVIVHENKTIV O34990 273 -1 - EKLVLARALKLVLEDRVFVHENKTVV Q9ZJI2 267 -1 - EKLVLARALKLVLEDRVFVHENKTVV O25975 267 -1 - ERVVLARAVRLHLQHRILVYDNRTVV PURU_SYNY3 257 -1 - ERRVLARALRYRLEDRVLLNGRKTVV Q9A2D5 252 -1 - EKVVLARAVKWHLEDKILVYNGKTVV O67681 256 -1 - ERAVLSRAVLWHCQDRVIVHHNQTIV PURU_MYCTU 284 -1 - ERRVLARALRYHLDDRVLLNGHKTVV Q9X7F7 259 -1 - ECKALSNAVRWHCEGRVFLYGNRTVV PURU_CORSP 259 -1 - ESLVLARAVSRHIEHRILLNGHRTVV Q9PCF0 256 -1 -
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