SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01571

Identifier
NPFFRECEPTR1  [View Relations]  [View Alignment]  
Accession
PR01571
No. of Motifs
7
Creation Date
13-SEP-2001
Title
Type 1 neuropeptide FF receptor signature
Database References
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. PANULA, P., AARNISALO, A.A. AND WASOWICZ, K.
Neuropeptide FF, a mammalian neuropeptide with multiple functions.
PROG.NEUROBIOL. 48(4-5) 461-487 (1996).
 
7. KOTANI, M., MOLLEREAU, C., DETHEUX, M., LE POUL, E., BREZILLON, S.,
VAKILI, J., MAZARGUIL, H., VASSART, G., ZAJAC, J.M. AND PARMENTIER, M.
Functional characterization of a human receptor for neuropeptide FF and
related peptides.
BR.J.PHARMACOL. 133 138-144 (2001).
 
8. BONINI, J.A., JONES, K.A., ADHAM, N., FORRAY, C., ARTYMYSHYN, R., DURKIN,
M.M., SMITH, K.E., TAMM, J.A., BOTEJU, L.W., LAKHLANI, P.P., RADDATZ, R.,
YAO, W.J., OGOZALEK, K.L., BOYLE, N., KOURANOVA, E.V., QUAN, Y., VAYSSE,
P.J., WETZEL, J.M., BRANCHEK, T.A., GERALD, C. AND BOROWSKY, B.
Identification and characterization of two G protein-coupled receptors for
neuropeptide FF.
J.BIOL.CHEM. 275(50) 39324-39331 (2000).
 
9. HINUMA, S., SHINTANI, Y., FUKUSUMI, S., IIJIMA, N., MATSUMOTO, Y.,
HOSOYA, M., FUJII, R., WATANABE, T., KIKUCHI, K., TERAO, Y., YANO, T.,
YAMAMOTO, T., KAWAMATA, Y., HABATA, Y., ASADA, M., KITADA, C., KUROKAWA, T.,
ONDA, H., NISHIMURA, O., TANAKA, M., IBATA, Y. AND FUJINO, M.
New neuropeptides containing carboxy-terminal RFamide and their receptor in
mammals.
NAT.CELL BIOL. 2(10) 703-708 (2000).

Documentation
G-protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine,
para-crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of which
transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the receptors
are very similar and are believed to adopt a common structural framework
comprising 7 transmembrane (TM) helices [3-5].
 
Neuropeptide FF (and neuropeptide AF, which is also derived from the same
precursor) belong to a family of neuropeptides containing an RF-amide motif
at their C-terminus [6]. Neuropeptide FF (NPFF) is found at high
concentrations in the posterior pituitary, spinal cord, hypothalamus and
medulla and is believed to be involved in pain modulation, opioid tolerance,
cardiovascular regulation, memory and neuroendocrine regulation [6,7]. Two G
protein-coupled receptors for NPFF have been identified; both are expressed
in the central nervous system and couple to Gi proteins to inhibit adenylyl
cyclase [7-9].
 
The type 1 neuropeptide FF receptor is expressed at highest levels in the
hypothalamus, with moderate expression in the thalamus, midbrain, medulla
oblongata, testis and eye [9].
 
NPFFRECEPTR1 is a 7-element fingerprint that provides a signature for the 
type 1 neuropeptide FF receptors. The fingerprint was derived from an 
initial alignment of 2 sequences: the motifs were drawn from conserved
sections within N- and C-terminal and loop regions, focusing on those areas 
of the alignment that characterise the type 1 neuropeptide FF receptors but
distinguish them from the rest of the neuropeptide FF receptor family -
motif 1 lies at the N-terminus; motif 2 resides in the second external loop;
motifs 3 and 4 span the third cytoplasmic loop; and motifs 5-7 reside at the
C-terminus. A single iteration on SPTR39.22_17.3f was required to reach
convergence, no further sequences being identified beyond the starting set.
Summary Information
2 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
72222222
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
NFF1_HUMAN    NFF1_RAT      
Sequence Titles
NFF1_HUMAN  NEUROPEPTIDE FF RECEPTOR 1 (RFAMIDE-RELATED PEPTIDE RECEPTOR OT7T022) - Homo sapiens (Human). 
NFF1_RAT NEUROPEPTIDE FF RECEPTOR 1 (RFAMIDE-RELATED PEPTIDE RECEPTOR OT7T022) - Rattus norvegicus (Rat).
Scan History
SPTR39.22_17.3f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
EPSQPPNSSWPLSQNG NFF1_HUMAN 4 4 -
EPSQPPNGSWPLGQNG NFF1_RAT 4 4 -

Motif 2 width=24
Element Seqn Id St Int Rpt
VTLTVTREEHHFMVDARNRSYPLY NFF1_HUMAN 178 158 -
VTLTVTREEHHFMLDARNRSYPLY NFF1_RAT 178 158 -

Motif 3 width=17
Element Seqn Id St Int Rpt
ARKLCQAPGPAPGGEEA NFF1_HUMAN 240 38 -
ARKLCQAPGPARDTEEA NFF1_RAT 240 38 -

Motif 4 width=11
Element Seqn Id St Int Rpt
RASRRRARVVH NFF1_HUMAN 260 3 -
RTSRRRARVVH NFF1_RAT 262 5 -

Motif 5 width=17
Element Seqn Id St Int Rpt
HKEAYSERPGGLLHRRV NFF1_HUMAN 356 85 -
HKQAYSERPNRLLRRRV NFF1_RAT 358 85 -

Motif 6 width=30
Element Seqn Id St Int Rpt
SDSGLPSESGPSSGAPRPGRLPLRNGRVAH NFF1_HUMAN 379 6 -
SDSGLPSESGPSSGVPGPGRLPLRNGRVAH NFF1_RAT 381 6 -

Motif 7 width=15
Element Seqn Id St Int Rpt
EGPGCSHLPLTIPAW NFF1_HUMAN 414 5 -
EGPGCNHMPLTIPAW NFF1_RAT 416 5 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
EPSQPPNSSWPLSQNG NFF1_HUMAN 4 4 -
EPSQPPNGSWPLGQNG NFF1_RAT 4 4 -

Motif 2 width=24
Element Seqn Id St Int Rpt
VTLTVTREEHHFMVDARNRSYPLY NFF1_HUMAN 178 158 -
VTLTVTREEHHFMLDARNRSYPLY NFF1_RAT 178 158 -

Motif 3 width=17
Element Seqn Id St Int Rpt
ARKLCQAPGPAPGGEEA NFF1_HUMAN 240 38 -
ARKLCQAPGPARDTEEA NFF1_RAT 240 38 -

Motif 4 width=11
Element Seqn Id St Int Rpt
RASRRRARVVH NFF1_HUMAN 260 3 -
RTSRRRARVVH NFF1_RAT 262 5 -

Motif 5 width=17
Element Seqn Id St Int Rpt
HKEAYSERPGGLLHRRV NFF1_HUMAN 356 85 -
HKQAYSERPNRLLRRRV NFF1_RAT 358 85 -

Motif 6 width=30
Element Seqn Id St Int Rpt
SDSGLPSESGPSSGAPRPGRLPLRNGRVAH NFF1_HUMAN 379 6 -
SDSGLPSESGPSSGVPGPGRLPLRNGRVAH NFF1_RAT 381 6 -

Motif 7 width=15
Element Seqn Id St Int Rpt
EGPGCSHLPLTIPAW NFF1_HUMAN 414 5 -
EGPGCNHMPLTIPAW NFF1_RAT 416 5 -