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PR01540

Identifier
INTRLEUKN1BP  [View Relations]  [View Alignment]  
Accession
PR01540
No. of Motifs
11
Creation Date
18-JUN-2001
Title
Interleukin-1 binding protein precursor signature 
Database References
PRINTS; PR01536 INTRLKN1R12F; PR01537 INTRLKN1R1F
Literature References
1. SIMS, J.E., MARCH, C.J., COSMAN, D., WIDMER, M.B., MACDONALD, H.R., 
MCMAHAN, C.J., GRUBIN, C.E., WIGNALL, J.M., JACKSON, J.L., CALL, S.M. ET AL..
cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin
superfamily.
SCIENCE 241 585-589 (1988).
 
2. LIU, C., HART, R.P., LIU, X.J., CLEVENGER, W., MAKI, R.A. 
AND DE SOUZA, E.B.
Cloning and characterization of an alternatively processed human type II
interleukin-1 receptor mRNA.
J.BIOL.CHEM. 271 20965-20972 (1996). 
 
3. MCMAHAN, C.J., SLACK, J.L., MOSLEY, B., COSMAN, D., LUPTON, S.D., 
BRUNTON, L.L., GRUBIN, C.E., WIGNALL, J.M., JENKINS, N.A., BRANNAN, C.I.
ET AL..
A novel IL-1 receptor, cloned from B cells by mammalian expression, is 
expressed in many cell types.
EMBO J. 10 2821-2832 (1991). 
 
4. SMITH, G.L. AND CHAN, Y.S.
Two vaccinia virus proteins structurally related to the interleukin-1 
receptor and the immunoglobulin superfamily. 
J.GEN.VIROL. 72 511-518 (1991). 
 
5. SPRIGGS, M.K., HRUBY, D.E., MALISZEWSKI, C.R., PICKUP, D.J., SIMS, J.E.,
BULLER, R.M. AND VANSLYKE, J.
Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding
protein.
CELL 71 145-152 (1992). 

Documentation
Interleukin-1 alpha and interleukin-1 beta (IL-1 alpha and IL-1 beta) are 
cytokines that participate in the regulation of immune responses, 
inflammatory reactions, and hematopoiesis [1]. Two types of IL-1 receptor, 
each with three extracellular immunoglobulin (Ig)-like domains, limited
sequence similarity (28%) and different pharmacological characteristics
have been cloned from mouse and human cell lines: these have been termed 
type I and type II receptors [2]. The receptors both exist in transmembrane
(TM) and soluble forms: the soluble IL-1 receptor is thought to be post-
translationally derived from cleavage of the extracellular portion of the
membrane receptors [2].
 
Both IL-1 receptors appear to be well conserved in evolution, and map to the
same chromosomal location [3]. The receptors can both bind all three forms 
of IL-1 (IL-1 alpha, IL-1 beta and IL-1RA) [3].
 
The vaccinia virus genes B15R and B18R each encode proteins with N-terminal 
hydrophobic sequences, possible sites for attachment of N-linked 
carbohydrate and a short C-terminal hydrophobic domain [4]. These properties
are consistent with the mature proteins being either virion, cell surface
or secretory glycoproteins. Protein sequence comparisons reveal that
the gene products are related to each other (20% identity) and to the Ig
superfamily [4]. The highest degree of similarity is to the human and murine
interleukin-1 receptors, although both proteins are related to a wide range
of Ig superfamily members, including the interleukin-6 receptor [4]. A novel
method for virus immune evasion has been proposed in which the product of 
one or both of these proteins may bind interleukin-1 and/or interleukin-6,
preventing these cytokines reaching their natural receptors [4]. A similar
gene product from cowpox virus has also been shown to specifically bind
murine IL-1 beta [5]. 
 
INTRLEUKN1BP is an 11-element fingerprint that provides a signature for the
interleukin-1 binding protein precursor. The fingerprint was derived from
an initial alignment of 3 sequences: the motifs were drawn from short
conserved regions spanning the N-terminal half of the alignment, focusing on
those sections that characterise the interleukin-1 binding protein precursor
but distinguish it from other members of the interleukin-1 receptor family - 
motifs 2 and 3 span Ig-like C2-type domain 1; motifs 5 and 6 reside within 
Ig-like C2-type domain 2; and motifs 8-10 span Ig-like C2-type domain 3.
Two iterations on SPTR39_15f were required to reach convergence, at which 
point a true set comprising 7 sequences was identified. Several partial 
matches were also found, all of which are Variola virus proteins that match
motifs 3 and 4 and 8 and 9 with a high degree of similarity.
Summary Information
   7 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
4 codes involving 2 elements
Composite Feature Index
1177777777777
1000000000000
900000000000
800000000000
700000000000
600000000000
500000000000
400000000000
300000000000
200220002200
1234567891011
True Positives
O57261        O72751        Q9JF38        Q9JFS8        
VB16_COWPX VB16_VACCC VB16_VACCV
True Positive Partials
Codes involving 2 elements
Q88988 Q89466 Q89477 Q89935
Sequence Titles
O57261      INTERLEUKIN-1 BETA RECEPTOR - Vaccinia virus. 
O72751 B14R PROTEIN - Cowpox virus (CPV).
Q9JF38 TB16R - Vaccinia virus (strain Tian Tan).
Q9JFS8 IL-1 BETA BINDING PROTEIN C9R - Ectromelia virus.
VB16_COWPX INTERLEUKIN-1 BINDING PROTEIN PRECURSOR (PROTEIN B16) - Cowpox virus (CPV).
VB16_VACCC INTERLEUKIN-1 BINDING PROTEIN PRECURSOR (PROTEIN B16) - Vaccinia virus (strain Copenhagen).
VB16_VACCV INTERLEUKIN-1 BINDING PROTEIN PRECURSOR (PROTEIN B16) - Vaccinia virus (strain WR).

Q88988 ORF14R - Variola virus, and variola minor virus.
Q89466 ORF16R - Variola virus, and variola minor virus.
Q89477 DNA COMPLETE GENOME - Variola virus.
Q89935 DNA COMPLETE GENOME - Variola virus.
Scan History
SPTR39_15f 2  30   NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
MELENEPVILP VB16_VACCC 1 1 -
MELENEPVILP VB16_VACCV 37 37 -
MELENEPVILP VB16_COWPX 37 37 -

Motif 2 width=16
Element Seqn Id St Int Rpt
QINTLSSGYNILDILW VB16_VACCC 14 2 -
QINTLSSGYNILDILW VB16_VACCV 50 2 -
QINTISSGYNILDILW VB16_COWPX 50 2 -

Motif 3 width=23
Element Seqn Id St Int Rpt
RGADNDRIIPIDNGSNMLILNPT VB16_VACCC 32 2 -
RGADNDRIIPIDNGSNMLILNPT VB16_VACCV 68 2 -
RGADNDRIIPIDNGSNMLILNPT VB16_COWPX 68 2 -

Motif 4 width=18
Element Seqn Id St Int Rpt
DMMSLNLTIVSVSESNID VB16_VACCC 72 17 -
DMMSLNLTIVSVSESNID VB16_VACCV 108 17 -
DMMSLNLTIVSVSESNID VB16_COWPX 108 17 -

Motif 5 width=16
Element Seqn Id St Int Rpt
NINAFIASNVNADIIW VB16_VACCC 109 19 -
NINAFIASNVNADIIW VB16_VACCV 145 19 -
NINAFIASNVNADIIW VB16_COWPX 145 19 -

Motif 6 width=23
Element Seqn Id St Int Rpt
SGHRRLRNKRLKQRTPGIITIED VB16_VACCC 125 0 -
SGHRRLRNKRLKQRTPGIITIED VB16_VACCV 161 0 -
SGHRRLRNKRLRQRTPGIITIED VB16_COWPX 161 0 -

Motif 7 width=21
Element Seqn Id St Int Rpt
KLEVRDKIIHPTMQLPEGVVT VB16_VACCC 176 28 -
KLEVRDKIIPSTMQLPDGIVT VB16_VACCV 212 28 -
KLEVRDRMIPPTMQLPDGVVT VB16_COWPX 212 28 -

Motif 8 width=17
Element Seqn Id St Int Rpt
RVSLRPPTTDADVFWIS VB16_VACCC 207 10 -
RVSLRPPTTDADVFWIS VB16_VACCV 243 10 -
RVSLRPPTTDADVFWIS VB16_COWPX 243 10 -

Motif 9 width=20
Element Seqn Id St Int Rpt
YYEEDDGDGDGRISVANKIY VB16_VACCC 227 3 -
YYEEDDGDGNGRISVANKIY VB16_VACCV 263 3 -
YYEEDDEDGDGRISVANKIY VB16_COWPX 263 3 -

Motif 10 width=20
Element Seqn Id St Int Rpt
TDKRRVITSRLNINPVKEED VB16_VACCC 248 1 -
TDKRRVITSRLNINPVKEED VB16_VACCV 284 1 -
TDKRRVITSRLKINPVKEED VB16_COWPX 284 1 -

Motif 11 width=23
Element Seqn Id St Int Rpt
ATTFTCMAFTIPSISKTVTVSIT VB16_VACCC 268 0 -
ATTFTCMAFTIPSISKTVTVSIT VB16_VACCV 304 0 -
ATTFTCMAFTIPSISKTVTISIT VB16_COWPX 304 0 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
MELENEPVILP O57261 37 37 -
MELENEPVILP VB16_VACCC 1 1 -
MELENEPVILP O72751 37 37 -
MELENEPVILP VB16_VACCV 37 37 -
MELENEPVILP Q9JF38 1 1 -
MELENEPVILP VB16_COWPX 37 37 -
MELEKEPVILP Q9JFS8 37 37 -

Motif 2 width=16
Element Seqn Id St Int Rpt
QINTLSSGYNILDILW O57261 50 2 -
QINTLSSGYNILDILW VB16_VACCC 14 2 -
QINTLSSGYNILDILW O72751 50 2 -
QINTLSSGYNILDILW VB16_VACCV 50 2 -
QINTLSSGYNILDILW Q9JF38 14 2 -
QINTISSGYNILDILW VB16_COWPX 50 2 -
QINTLSSGYNVLDIIW Q9JFS8 50 2 -

Motif 3 width=23
Element Seqn Id St Int Rpt
RGADNDRIIPIDNGSNMLILNPT O57261 68 2 -
RGADNDRIIPIDNGSNMLILNPT VB16_VACCC 32 2 -
RGADNDRIIPIDNGSNMLILNPT O72751 68 2 -
RGADNDRIIPIDNGSNMLILNPT VB16_VACCV 68 2 -
RGADNDRIIPIDNGSNMLILNPT Q9JF38 32 2 -
RGADNDRIIPIDNGSNMLILNPT VB16_COWPX 68 2 -
RGADNDNIIPIDNGTNMLILNPT Q9JFS8 68 2 -

Motif 4 width=18
Element Seqn Id St Int Rpt
DMMSLNLTIVSVSESNID O57261 108 17 -
DMMSLNLTIVSVSESNID VB16_VACCC 72 17 -
DMMSLNLTIVSVSESNID O72751 108 17 -
DMMSLNLTIVSVSESNID VB16_VACCV 108 17 -
DMMSLNLTIVSVLESNID Q9JF38 72 17 -
DMMSLNLTIVSVSESNID VB16_COWPX 108 17 -
DMMSLNLTIVTDTESNID Q9JFS8 108 17 -

Motif 5 width=16
Element Seqn Id St Int Rpt
NINAFIASNVNADIIW O57261 145 19 -
NINAFIASNVNADIIW VB16_VACCC 109 19 -
NINAFIASNVNADIIW O72751 145 19 -
NINAFIASNVNADIIW VB16_VACCV 145 19 -
NINAFIASNVNADIIW Q9JF38 109 19 -
NINAFIASNVNADIIW VB16_COWPX 145 19 -
NINAFIARNKDVDVDV Q9JFS8 145 19 -

Motif 6 width=23
Element Seqn Id St Int Rpt
SGHRRLRNKRLKQRTPGIITIED O57261 161 0 -
SGHRRLRNKRLKQRTPGIITIED VB16_VACCC 125 0 -
SGHRRLRNKRLKQRTPGIITIED O72751 161 0 -
SGHRRLRNKRLKQRTPGIITIED VB16_VACCV 161 0 -
SGHRRLRNKRLKQRTPGIITIED Q9JF38 125 0 -
SGHRRLRNKRLRQRTPGIITIED VB16_COWPX 161 0 -
RGHPRLRNKRLTQRPIGIITIED Q9JFS8 163 2 -

Motif 7 width=21
Element Seqn Id St Int Rpt
KLEVRDKIIPSTMQLPEGVVT O57261 212 28 -
KLEVRDKIIHPTMQLPEGVVT VB16_VACCC 176 28 -
KLEVRDRIIHPTMQLPEGVVT O72751 212 28 -
KLEVRDKIIPSTMQLPDGIVT VB16_VACCV 212 28 -
KLEVRDKIIPSTMQLPDGIVT Q9JF38 176 28 -
KLEVRDRMIPPTMQLPDGVVT VB16_COWPX 212 28 -
KLEVRDRIIPPTMQLPVVVMT Q9JFS8 214 28 -

Motif 8 width=17
Element Seqn Id St Int Rpt
RVSLRPPTTDADVFWIS O57261 243 10 -
RVSLRPPTTDADVFWIS VB16_VACCC 207 10 -
RVSLRPPTTDADVFWIS O72751 243 10 -
RVSLRPPTTDADVFWIS VB16_VACCV 243 10 -
RVSLRPPTTDADVFWIS Q9JF38 207 10 -
RVSLRPPTTDADVFWIS VB16_COWPX 243 10 -
RVSLRPPTIDADVFWIS Q9JFS8 245 10 -

Motif 9 width=20
Element Seqn Id St Int Rpt
YYEEDDGDGDGRISVANKIY O57261 263 3 -
YYEEDDGDGDGRISVANKIY VB16_VACCC 227 3 -
YYEEDDGDGDGRISVANKIY O72751 263 3 -
YYEEDDGDGNGRISVANKIY VB16_VACCV 263 3 -
YYEEDDGDGNGRISVANKIY Q9JF38 227 3 -
YYEEDDEDGDGRISVANKIY VB16_COWPX 263 3 -
YYEEDDEDSDGRSVTNRIFT Q9JFS8 265 3 -

Motif 10 width=20
Element Seqn Id St Int Rpt
TDKRRVITSRLNINPVKEED O57261 284 1 -
TDKRRVITSRLNINPVKEED VB16_VACCC 248 1 -
TDKRRVITSRLKINPVKEED O72751 284 1 -
TDKRRVITSRLNINPVKEED VB16_VACCV 284 1 -
TDKRRVITSRLNINPVKEED Q9JF38 248 1 -
TDKRRVITSRLKINPVKEED VB16_COWPX 284 1 -
IDKRRVITSRLNINPVKEDD Q9JFS8 285 0 -

Motif 11 width=23
Element Seqn Id St Int Rpt
ATTFTCMAFTIPSISKTVTVSIT O57261 304 0 -
ATTFTCMAFTIPSISKTVTVSIT VB16_VACCC 268 0 -
ATTFTCMAFTIPSISKTVTVSIT O72751 304 0 -
ATTFTCMAFTIPSISKTVTVSIT VB16_VACCV 304 0 -
ATTFTCMAFTIPSISKTVTVSIT Q9JF38 268 0 -
ATTFTCMAFTIPSISKTVTISIT VB16_COWPX 304 0 -
TTFTCMAFTVHNSISKTVTISIK Q9JFS8 306 1 -