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PR01518

Identifier
KV43CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01518
No. of Motifs
7
Creation Date
19-JUN-2001
Title
Kv4.3 voltage-gated K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01491 KVCHANNEL; PR01497 SHALCHANNEL
Literature References
1. MILLER, C.
An overview of the potassium channel family.
GENOME BIOL. 1(4) 1-5 (2000).
 
2. ASHCROFT, F.M.
Voltage-gated K+ channels.
IN ION CHANNELS AND DISEASE, ACADEMIC PRESS, 2000, PP.97-123.
 
3. SANSOM, M.S.
Putting the parts together.
CURR.BIOL. 9(19) R738-R741 (1999).   
 
4. CONLEY, E.C. AND BRAMMER, W.J.
Shal.
IN THE ION CHANNEL FACTSBOOK, VOLUME IV, ACADEMIC PRESS, 1999, PP.617-646.
 
5. DANIEL, D., LING, H.P., COCKETT, M. SOKOL, P. AND NUMANN, R.
Cloning and Expression of the Human Kv4.3 Potassium Channel.
J.NEUROPHYSIOL. 81(4) 1974-1977 (1999).
 
6. YANG, E.K., ALVIRA, M.R., LEVITAN, E.S. AND TAKIMOTO, K.
Kvbeta subunits increase expression of kv4.3 channels by interacting with
their C-termini.
J.BIOL.CHEM. 276(7) 4839-4844 (2001).

Documentation
Potassium ion (K+) channels are a structurally diverse group of proteins
that facilitate the flow of K+ ions across cell membranes. They are
ubiquitous, being present in virtually all cell types. Activation of K+
channels tends to hyperpolarise cells, reducing the membrane's electrical
resistance, dampening nervous activity. In eukaryotic cells, K+ channels are
involved in neural signalling and generation of the cardiac rhythm, and
act as effectors in signal transduction pathways involving G protein-
coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
maintenance of ionic homeostasis [1].
      
Structurally, Kv channels belong to the subfamily of K+ channels whose
subunits contain 6 transmembrane (TM) domains: these are the voltage-
gated K+ (Kv) channels, the KCNQ channels, the EAG-like K+ channels and
3 kinds of Ca2+-activated K+ channels (BK, IK and SK) [2]. All K+
channels share a characteristic sequence feature: a TMxTVGYG motif
that resides between the 2 C-terminal membrane spanning helices, and
forms the K+-selective pore domain [1-2].
     
The Kv family can be divided into 4 subfamilies on the basis of sequence
similarity and function: Shaker (Kv1), Shab (Kv2), Shaw (Kv3) and Shal 
(Kv4). All consist of pore-forming alpha subunits that associate with 
different types of beta subunit. To form a functional K+ channel pore,
4 alpha subunits and 4 beta subunits are required. The alpha subunits
have 6 well-conserved TM domains, a pore loop region and variable length
N- and C-termini. TM domains 5 and 6, and the loop region, form the K+ 
channel pore through which the K+ ions pass [3].
     
The first Shal (Kv4) sequence was found in Drosophila. Several vertebrate
K+ channels with similar amino acid sequences were subsequently found and,
together with the Drosophila Shal channel, now constitute the Shal (Kv4)
family. These channels support outward K+-selective currents and are
inhibited by free fatty acids [4]. The Shal family can be further divided 
into 3 families, designated Kv4.1, Kv4.2 and Kv4.3.
    
Two isoforms of Kv4.3 have been cloned: one is full length and the other has
a small amino acid deletion. Both forms are expressed in the brain, whereas
in the heart only the longer isoform is found [5]. Kv4.3 channels may have
an important role in damping synaptic and excitatory membrane potentials [5]. 
In the brain, they can also associate with Kv-beta2 subunits via the C-
terminus, resulting in increased channel density and protein expression [6].
 
KV43CHANNEL is a 7-element fingerprint that provides a signature for the
Kv4.3 voltage-gated K+ channel. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions 
spanning the full alignment length, focusing on those sections that 
characterise the Kv4.3 channels but distinguish them from other members of
the Shal family - motifs 1 and 2 reside at the N-terminus; and motifs 3-7
span the C-terminus. Two iterations on SPTR39_15f were required to reach
convergence, at which point a true set comprising 15 sequences was
identified.
Summary Information
15 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
715151515151515
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
O08723        O57662        O60576        O60577        
P70622 Q62897 Q63286 Q9PTD3
Q9TTT5 Q9UH85 Q9UH86 Q9UK16
Q9UK17 Q9Z0V0 Q9Z0V1
Sequence Titles
O08723      KV4.3 - Rattus norvegicus (Rat).              
O57662 POTASSIUM CHANNEL XKV4.3 - Xenopus laevis (African clawed frog).
O60576 KV4.3 POTASSIUM CHANNEL SHORT SPLICE VARIANT - Homo sapiens (Human).
O60577 KV4.3 POTASSIUM CHANNEL LONG SPLICE VARIANT - Homo sapiens (Human).
P70622 KV4.3 POTASSIUM CHANNEL - Rattus norvegicus (Rat).
Q62897 SHAL-RELATED POTASSIUM CHANNEL KV4.3 - Rattus norvegicus (Rat).
Q63286 VOLTAGE-GATED K+ CHANNEL - Rattus norvegicus (Rat).
Q9PTD3 POTASSIUM CHANNEL KV4.3 - Gallus gallus (Chicken).
Q9TTT5 POTASSIUM CHANNEL KV4.3 - Oryctolagus cuniculus (Rabbit).
Q9UH85 POTASSIUM IONIC CHANNEL KV4.3 LONG ISOFORM (VOLTAGE-GATED POTASSIUM CHANNEL KV4.3 LONG VARIANT) - Homo sapiens (Human).
Q9UH86 POTASSIUM IONIC CHANNEL KV4.3 SHORT ISOFORM (VOLTAGE-GATED POTASSIUM CHANNEL KV4.3 SHORT VARIANT) - Homo sapiens (Human).
Q9UK16 VOLTAGE GATED POTASSIUM CHANNEL KV4.3 SHORT SPLICE VARIANT - Homo sapiens (Human).
Q9UK17 VOLTAGE GATED POTASSIUM CHANNEL KV4.3 LONG SPLICE VARIANT - Homo sapiens (Human).
Q9Z0V0 POTASSIUM CHANNEL KV4.3M - Mus musculus (Mouse).
Q9Z0V1 POTASSIUM CHANNEL KV4.3L - Mus musculus (Mouse).
Scan History
SPTR39_15f 2  180  NSINGLE    
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
NCPMPLAPADKNKRQDE P70622 24 24 -
NCPMPLAPADKNKRQDE Q9UH85 24 24 -
NCPMPLAPADKNKRQDE Q9Z0V0 24 24 -
NCPMPLAPADKSKRQDE Q9TTT5 24 24 -
NCPMPLAPADKNKRQDE Q9UK16 24 24 -
NCPMPLAPTEKNKRQDE Q9PTD3 24 24 -
NCPMPLAPADKNKKQDE O57662 24 24 -

Motif 2 width=12
Element Seqn Id St Int Rpt
LMDDNDSENNQE Q9UH85 147 106 -
LMDDNESENNQE P70622 147 106 -
LMDDNDSENNQE Q9Z0V0 147 106 -
LMDDNDSENNQE Q9TTT5 147 106 -
LMDDNDSENNQE Q9UK16 147 106 -
LMDDNDSENNQE Q9PTD3 147 106 -
LMDDNEFENKQE O57662 147 106 -

Motif 3 width=14
Element Seqn Id St Int Rpt
GLLNEALELTGTPE Q9UH85 448 289 -
GLLNEALELTGTPE P70622 448 289 -
GLLNEALELTGTPE Q9Z0V0 448 289 -
GLLNEALELTGTPE Q9TTT5 448 289 -
GLLNGALELTGTPE Q9UK16 448 289 -
GLLNEALELTGSTE Q9PTD3 449 290 -
GLLNEALELMGSTE O57662 453 294 -

Motif 4 width=13
Element Seqn Id St Int Rpt
QMGKTTSLIESQH Q9Z0V0 464 2 -
HMGKTTSLIESQH Q9UH85 464 2 -
HMGKTTSLIESQH P70622 464 2 -
HMGKTASLIESQH Q9TTT5 464 2 -
HMGKTTSLIESQH Q9UK16 464 2 -
HTTKGTSLIESQH Q9PTD3 466 3 -
HIGKSASLIESQH O57662 469 2 -

Motif 5 width=13
Element Seqn Id St Int Rpt
PNSNLPATRLRSM Q9UH85 558 81 -
PNSNLPATRLRSM P70622 539 62 -
PNSNLPATRLRSM Q9Z0V0 539 62 -
PNSNLPATRLRSM Q9TTT5 558 81 -
PNSNLPDTRLRSM Q9UK16 539 62 -
PNSSVPATRLRSM Q9PTD3 560 81 -
PNSNVPATRLRTM O57662 564 82 -

Motif 6 width=12
Element Seqn Id St Int Rpt
DQPSLTASRSSL O57662 588 11 -
EQPSLTTSRSSL P70622 563 11 -
EQPSLTTSRSSL Q9Z0V0 563 11 -
EQPSLTTSRSSL Q9UH85 582 11 -
EQPSLTTSRSSL Q9TTT5 582 11 -
EQPSLTTSRSSL Q9UK16 563 11 -
EQPSLTTSRSSL Q9PTD3 584 11 -

Motif 7 width=15
Element Seqn Id St Int Rpt
MKSDDGLRPNCKAAQ Q9PTD3 597 1 -
LKADDGLRPNCKTSQ Q9UH85 595 1 -
LKADDGLRPNCKTSQ P70622 576 1 -
LKADDGLRPNCKTSQ Q9Z0V0 576 1 -
LKADDGLRPNCKTSQ Q9TTT5 595 1 -
LKADDGLRPNCKTSQ Q9UK16 576 1 -
LKSEDEMRSNCKASQ O57662 601 1 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
NCPMPLAPADKNKRQDE Q9UH86 24 24 -
NCPMPLAPADKNKRQDE Q9UH85 24 24 -
NCPMPLAPADKNKRQDE O60577 24 24 -
NCPMPLAPADKNKRQDE O08723 24 24 -
NCPMPLAPADKNKRQDE P70622 24 24 -
NCPMPLAPADKNKRQDE O60576 24 24 -
NCPMPLAPADKNKRQDE Q62897 24 24 -
NCPMPLAPADKNKRQDE Q9Z0V0 24 24 -
NCPMPLAPADKNKRQDE Q9Z0V1 24 24 -
NCPMPLAPADKSKRQDE Q9TTT5 24 24 -
NCPMPLAPADKNKRQDE Q9UK16 24 24 -
NCPMPLAPADKNKRQDE Q9UK17 24 24 -
NCPMPLAPADKNKRQDE Q63286 24 24 -
NCPMPLAPTEKNKRQDE Q9PTD3 24 24 -
NCPMPLAPADKNKKQDE O57662 24 24 -

Motif 2 width=12
Element Seqn Id St Int Rpt
LMDDNDSENNQE O60576 147 106 -
LMDDNDSENNQE Q9UH86 147 106 -
LMDDNDSENNQE Q9UH85 147 106 -
LMDDNDSENNQE O60577 147 106 -
LMDDNESENNQE O08723 147 106 -
LMDDNESENNQE P70622 147 106 -
LMDDNESENNQE Q62897 147 106 -
LMDDNDSENNQE Q9Z0V0 147 106 -
LMDDNDSENNQE Q9Z0V1 147 106 -
LMDDNDSENNQE Q9TTT5 147 106 -
LMDDNDSENNQE Q9UK16 147 106 -
LMDDNDSENNQE Q9UK17 147 106 -
LMDDNESENNQE Q63286 147 106 -
LMDDNDSENNQE Q9PTD3 147 106 -
LMDDNEFENKQE O57662 147 106 -

Motif 3 width=14
Element Seqn Id St Int Rpt
GLLNEALELTGTPE O60576 448 289 -
GLLNEALELTGTPE Q9UH86 448 289 -
GLLNEALELTGTPE Q9UH85 448 289 -
GLLNEALELTGTPE O60577 448 289 -
GLLNEALELTGTPE O08723 448 289 -
GLLNEALELTGTPE P70622 448 289 -
GLLNEALELTGTPE Q62897 448 289 -
GLLNEALELTGTPE Q9Z0V0 448 289 -
GLLNEALELTGTPE Q9Z0V1 448 289 -
GLLNEALELTGTPE Q9TTT5 448 289 -
GLLNGALELTGTPE Q9UK16 448 289 -
GLLNGALELTGTPE Q9UK17 448 289 -
GLLNEALELTGTPE Q63286 448 289 -
GLLNEALELTGSTE Q9PTD3 449 290 -
GLLNEALELMGSTE O57662 453 294 -

Motif 4 width=13
Element Seqn Id St Int Rpt
HMGKTTSLIESQH Q62897 464 2 -
HMGKTTSLIESQH O60576 464 2 -
HMGKTTSLIESQH Q9UH86 464 2 -
HMGKTTSLIESQH Q9UH85 464 2 -
HMGKTTSLIESQH O60577 464 2 -
HMGKTTSLIESQH O08723 464 2 -
HMGKTTSLIESQH P70622 464 2 -
QMGKTTSLIESQH Q9Z0V0 464 2 -
QMGKTTSLIESQH Q9Z0V1 464 2 -
HMGKTASLIESQH Q9TTT5 464 2 -
HMGKTTSLIESQH Q9UK16 464 2 -
HMGKTTSLIESQH Q9UK17 464 2 -
HMGKTTSLIESQH Q63286 464 2 -
HTTKGTSLIESQH Q9PTD3 466 3 -
HIGKSASLIESQH O57662 469 2 -

Motif 5 width=13
Element Seqn Id St Int Rpt
PNSNVPATRLRTM O57662 564 82 -
PNSNLPATRLRSM O60576 539 62 -
PNSNLPATRLRSM Q9UH86 539 62 -
PNSNLPATRLRSM Q9UH85 558 81 -
PNSNLPATRLRSM O60577 558 81 -
PNSNLPATRLRSM O08723 558 81 -
PNSNLPATRLRSM P70622 539 62 -
PNSNLPATRLRSM Q62897 539 62 -
PNSNLPATRLRSM Q9Z0V0 539 62 -
PNSNLPATRLRSM Q9Z0V1 558 81 -
PNSNLPATRLRSM Q9TTT5 558 81 -
PNSNLPDTRLRSM Q9UK16 539 62 -
PNSNLPDTRLRSM Q9UK17 558 81 -
PNSNLPATRLRTM Q63286 539 62 -
PNSSVPATRLRSM Q9PTD3 560 81 -

Motif 6 width=12
Element Seqn Id St Int Rpt
EQPSLTTSRSSL Q63286 563 11 -
EQPSLTTSRSSL O60576 563 11 -
EQPSLTTSRSSL Q9UH86 563 11 -
EQPSLTTSRSSL O60577 582 11 -
EQPSLTTSRSSL O08723 582 11 -
EQPSLTTSRSSL P70622 563 11 -
EQPSLTTSRSSL Q62897 563 11 -
EQPSLTTSRSSL Q9Z0V0 563 11 -
EQPSLTTSRSSL Q9Z0V1 582 11 -
EQPSLTTSRSSL Q9TTT5 582 11 -
EQPSLTTSRSSL Q9UK16 563 11 -
EQPSLTTSRSSL Q9UK17 582 11 -
EQPSLTTSRSSL Q9PTD3 584 11 -
DQPSLTASRSSL O57662 588 11 -
EQPSLTTSRSSL Q9UH85 582 11 -

Motif 7 width=15
Element Seqn Id St Int Rpt
LKADDGLRPNCKTSQ O60576 576 1 -
LKADDGLRPNCKTSQ Q9UH86 576 1 -
LKADDGLRPNCKTSQ Q9UH85 595 1 -
LKADDGLRPNCKTSQ O60577 595 1 -
LKADDGLRPNCKTSQ O08723 595 1 -
LKADDGLRPNCKTSQ P70622 576 1 -
LKADDGLRPNCKTSQ Q62897 576 1 -
MKSDDGLRPNCKAAQ Q9PTD3 597 1 -
LKADDGLRPNCKTSQ Q9Z0V0 576 1 -
LKADDGLRPNCKTSQ Q9Z0V1 595 1 -
LKADDGLRPNCKTSQ Q9TTT5 595 1 -
LKADDGLRPNCKTSQ Q9UK16 576 1 -
LKSEDEMRSNCKASQ O57662 601 1 -
LKADDGLRPNCKTSQ Q9UK17 595 1 -
LKADDGLRPNCKTQD Q63286 576 1 -