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PR01512

Identifier
KV15CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01512
No. of Motifs
8
Creation Date
21-JUN-2001
Title
Kv1.5 voltage-gated K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01491 KVCHANNEL; PR01496 SHAKERCHANEL
Literature References
1. MILLER, C.
An overview of the potassium channel family.
GENOME BIOL. 1(4) 1-5 (2000).
 
2. ASHCROFT, F.M.
Voltage-gated K+ channels.
IN ION CHANNELS AND DISEASE, ACADEMIC PRESS, 2000, PP.97-123.
 
3. SANSOM, M.S.
Putting the parts together.
CURR.BIOL. 9(19) R738-R741 (1999).   
 
4. LITTLETON, J.T. AND GANETZKY, B.
Ion Channels and synaptic organisation: analysis of the Drosophila genome.  
NEURON 26 35-43 (2000).
 
5. CONLEY, E.C. AND BRAMMAR, W.J.
Shaker.
IN THE ION CHANNEL FACTSBOOK, VOLUME IV, ACADEMIC PRESS, 1999, PP.559-614.	
 
6. FENG, J., WIBLE, B., LI, G.R., WANG, Z. AND NATTEL, S.
Antisense oligodeoxynucleotides directed against Kv1.5 mRNA specifically
inhibit ultrarapid delayed rectifier K+ current in cultured adult human
atrial myocytes.
CIRC.RES. 80 572-579 (1997).
 
7. MAYS, D.J., FOOSE, J.M., PHILIPSON, L.H. AND TAMKUN, M.M.
Localization of the Kv1.5 K+ channel protein in explanted cardiac tissue.
J.CLIN.INVEST. 96 282-292 (1995).
 
8. JAGER, H. AND GRISSMER, S.
Regulation of a mammalian Shaker-related potassium channel, hKv1.5, by
extracellular potassium and pH.
FEBS LETT. 488 (1-2) 45-50 (2001).

Documentation
Potassium ion (K+) channels are a structurally diverse group of proteins
that facilitate the flow of K+ ions across cell membranes. They are
ubiquitous, being present in virtually all cell types. Activation of K+
channels tends to hyperpolarise cells, reducing the membrane's electrical
resistance, dampening nervous activity. In eukaryotic cells, K+ channels
are involved in neural signalling and generation of the cardiac rhythm, and
act as effectors in signal transduction pathways involving G protein-
coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
maintenance of ionic homeostasis [1].
      
Structurally, Kv channels belong to the subfamily of K+ channels whose
subunits contain 6 transmembrane (TM) domains: these are the voltage-
gated K+ (Kv) channels, the KCNQ channels, the EAG-like K+ channels and
3 kinds of Ca2+-activated K+ channels (BK, IK and SK) [2]. All K+
channels share a characteristic sequence feature: a TMxTVGYG motif
that resides between the 2 C-terminal membrane spanning helices, and
forms the K+-selective pore domain [1-2].
     
The Kv family can be divided into 4 subfamilies on the basis of sequence
similarity and function: Shaker (Kv1), Shab (Kv2), Shaw (Kv3) and Shal 
(Kv4). All consist of pore-forming alpha subunits that associate with 
different types of beta subunit. To form a functional K+ channel pore,
4 alpha subunits and 4 beta subunits are required. The alpha subunits
have 6 well-conserved TM domains, a pore loop region and variable length
N- and C-termini. TM domains 5 and 6, and the loop region, form the K+ 
channel pore through which the K+ ions pass [3]. 
     
The first Shaker (Kv1) sequence was found in Drosophila. Several vertebrate
K+ channels with similar amino acid sequences were subsequently found and,
together with the Drosophila Shaker channel, now constitute the Shaker (Kv1)
family. These channels are mostly expressed in the brain, but can also be
found in non-excitable cells, such as lymphocytes [4,5].  
 
Kv1.5 channels are expressed in the heart and pancreatic beta-cells. The
most studied is the human Kv1.5 channel (hKv1.5), which is thought to
underlie the ultra-rapidly activating delayed rectifier K+ current Ikur
found in human atrial myocytes [6]. It is also expressed in the human
ventricle where it is possible that it contributes to the K+ current through
formation of heteromultimeric K+ channels with other Kv-alpha subunits [7].
Further experiments have shown that it is regulated by extracellular
potassium and pH [8].
 
KV15CHANNEL is an 8-element fingerprint that provides a signature for the
Kv1.5 voltage-gated potassium channel. The fingerprint was derived from an
initial alignment of 4 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length - motifs 1-7 reside at
the N-terminus; and motif 8 lies at the C-terminus. Two iterations on 
SPTR39_15f were required to reach convergence, at which point a true set 
comprising 7 sequences was identified.
Summary Information
7 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
877777777
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
CIK5_HUMAN    CIK5_MOUSE    CIK5_MUSPF    CIK5_RABIT    
CIK5_RAT Q9TS07 Q9Z1R6
Sequence Titles
CIK5_HUMAN  VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV1.5 (HK2) (HPCN1) - Homo sapiens (Human). 
CIK5_MOUSE VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV1.5 (KV1-5) - Mus musculus (Mouse).
CIK5_MUSPF VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV1.5 - Mustela putorius furo (Ferret).
CIK5_RABIT VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV1.5 - Oryctolagus cuniculus (Rabbit).
CIK5_RAT VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV1.5 (KV1) (RCK7) - Rattus norvegicus (Rat).
Q9TS07 BAK5=DELAYED RECTIFIER POTASSIUM CHANNEL KV1.5 HOMOLOG - Bos taurus (Bovine).
Q9Z1R6 VENTRICULAR POTASSIUM CHANNEL KV1.5 - Mus musculus (Mouse).
Scan History
SPTR39_15f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
MEIALVPLENGG CIK5_MUSPF 1 1 -
MEISLVPMENGS Q9Z1R6 1 1 -
MEISLVPLENGS CIK5_RAT 1 1 -
MEIALGPLENGG CIK5_RABIT 1 1 -

Motif 2 width=15
Element Seqn Id St Int Rpt
MTVRGGGEAGTGCSQ CIK5_MUSPF 14 1 -
MTLRGGGEAGASCVQ Q9Z1R6 14 1 -
MTLRGGGEAGASCVQ CIK5_RAT 14 1 -
MTIRGGGEETAGCSQ CIK5_RABIT 14 1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
PTAGLSDGPKEPAP CIK5_MUSPF 38 9 -
PTAGLNNQSKETSP Q9Z1R6 38 9 -
PTSGLNNQSKETLL CIK5_RAT 38 9 -
PTAGLGDGSQEPAP CIK5_RABIT 31 2 -

Motif 4 width=14
Element Seqn Id St Int Rpt
GTQRGVDPGGRPLP CIK5_MUSPF 55 3 -
ATHEDAGQGGRPLP Q9Z1R6 55 3 -
TTLEDANQGGRPLP CIK5_RAT 55 3 -
SARRGAEPGERPLP CIK5_RABIT 50 5 -

Motif 5 width=16
Element Seqn Id St Int Rpt
PRRLPPEDEEGEGDPA CIK5_MUSPF 77 8 -
PRRPSAEDEEGEGDPG Q9Z1R6 77 8 -
PRRLSAEDEEGEGDPG CIK5_RAT 77 8 -
SRRSPLEEEEGEGDPG CIK5_RABIT 72 8 -

Motif 6 width=13
Element Seqn Id St Int Rpt
SGLRFETQLGTLA CIK5_MUSPF 116 23 -
SGLRFETQLGTLA Q9Z1R6 118 25 -
SGLRFETQLGTLA CIK5_RAT 118 25 -
SGLRFETQLGTLA CIK5_RABIT 111 23 -

Motif 7 width=13
Element Seqn Id St Int Rpt
QAALKEEQGSQSH CIK5_MUSPF 520 391 -
QAALKEEQGIQRR Q9Z1R6 521 390 -
QAALKEEQGNQRR CIK5_RAT 521 390 -
QAALKEEPGSQSR CIK5_RABIT 517 393 -

Motif 8 width=15
Element Seqn Id St Int Rpt
GSCPLEKCNLKAKSN CIK5_MUSPF 567 34 -
GSCPLEKCHLKAKSN Q9Z1R6 568 34 -
GSCPLEKCHLKAKSN CIK5_RAT 568 34 -
GSCLLEKYNLKAKSN CIK5_RABIT 564 34 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
MEIALVPLENGG CIK5_MUSPF 1 1 -
MEISLVPMENGS Q9Z1R6 1 1 -
MEISLVPTENGS CIK5_MOUSE 1 1 -
MEISLVPLENGS CIK5_RAT 1 1 -
MEIALVPLENGG CIK5_HUMAN 1 1 -
MEIALGPLENGG CIK5_RABIT 1 1 -
MEIALVPLENGG Q9TS07 1 1 -

Motif 2 width=15
Element Seqn Id St Int Rpt
MTVRGGGEAGTGCSQ CIK5_MUSPF 14 1 -
MTLRGGGEAGASCVQ Q9Z1R6 14 1 -
MTLRGGGEAVASCVQ CIK5_MOUSE 14 1 -
MTLRGGGEAGASCVQ CIK5_RAT 14 1 -
MTVRGGDEARAGCGQ CIK5_HUMAN 14 1 -
MTIRGGGEETAGCSQ CIK5_RABIT 14 1 -
MTVRGEEEARTTAGQ Q9TS07 14 1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
PTAGLSDGPKEPAP CIK5_MUSPF 38 9 -
PTAGLNNQSKETSP Q9Z1R6 38 9 -
PTAGLNNQSKETSP CIK5_MOUSE 38 9 -
PTSGLNNQSKETLL CIK5_RAT 38 9 -
PTAGLSDGPKEPAP CIK5_HUMAN 38 9 -
PTAGLGDGSQEPAP CIK5_RABIT 31 2 -
TTAALSDGPKQPAP Q9TS07 33 4 -

Motif 4 width=14
Element Seqn Id St Int Rpt
GTQRGVDPGGRPLP CIK5_MUSPF 55 3 -
ATHEDAGQGGRPLP Q9Z1R6 55 3 -
ATHEDAAQGGRPLP CIK5_MOUSE 55 3 -
TTLEDANQGGRPLP CIK5_RAT 55 3 -
GAQRDADSGVRPLP CIK5_HUMAN 55 3 -
SARRGAEPGERPLP CIK5_RABIT 50 5 -
GGERGADPGGRPAP Q9TS07 51 4 -

Motif 5 width=16
Element Seqn Id St Int Rpt
PRRLPPEDEEGEGDPA CIK5_MUSPF 77 8 -
PRRPSAEDEEGEGDPG Q9Z1R6 77 8 -
PRRPSAEDEEGEGDPG CIK5_MOUSE 77 8 -
PRRLSAEDEEGEGDPG CIK5_RAT 77 8 -
PRRPPPEDEEEEGDPG CIK5_HUMAN 88 19 -
SRRSPLEEEEGEGDPG CIK5_RABIT 72 8 -
ASPRPPEEEDGEDDPA Q9TS07 73 8 -

Motif 6 width=13
Element Seqn Id St Int Rpt
SGLRFETQLGTLA CIK5_MUSPF 116 23 -
SGLRFETQLGTLA Q9Z1R6 118 25 -
SGLRFETQLDTLV CIK5_MOUSE 118 25 -
SGLRFETQLGTLA CIK5_RAT 118 25 -
SGLRFETQLGTLA CIK5_HUMAN 127 23 -
SGLRFETQLGTLA CIK5_RABIT 111 23 -
SGLRFETQLGTLA Q9TS07 112 23 -

Motif 7 width=13
Element Seqn Id St Int Rpt
QAALKEEQGSQSH CIK5_MUSPF 520 391 -
QAALKEEQGIQRR Q9Z1R6 521 390 -
QAALKEEQGIQRR CIK5_MOUSE 521 390 -
QAALKEEQGNQRR CIK5_RAT 521 390 -
PAVLKEEQGTQSQ CIK5_HUMAN 532 392 -
QAALKEEPGSQSR CIK5_RABIT 517 393 -
PAAVKEEQGSQSQ Q9TS07 516 391 -

Motif 8 width=15
Element Seqn Id St Int Rpt
GSCPLEKCNLKAKSN CIK5_MUSPF 567 34 -
GSCPLEKCHLKAKSN Q9Z1R6 568 34 -
GSCPLEKCHLKAKSN CIK5_MOUSE 568 34 -
GSCPLEKCHLKAKSN CIK5_RAT 568 34 -
GSCPLEKCNVKAKSN CIK5_HUMAN 579 34 -
GSCLLEKYNLKAKSN CIK5_RABIT 564 34 -
GSCSLEKCNLKAKSN Q9TS07 563 34 -