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PR01494

Identifier
KV9CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01494
No. of Motifs
7
Creation Date
15-MAR-2001
Title
Kv9 potassium channel alpha subunit signature
Database References
PRINTS; PR00169 KCHANNEL; PR01491 KVCHANNEL
Literature References
1. MILLER, C.
An overview of the potassium channel family.
GENOME BIOL. 1(4) 1-5 (2000).
 
2. ASHCROFT, F.M.
Voltage-gated K+ channels.
IN ION CHANNELS AND DISEASE, ACADEMIC PRESS, 2000, PP.97-123.
 
3. SANSOM, M.S.
Putting the parts together.
CURR.BIOL. 9(19) R738-R741 (1999).   
 
4. SALINAS, M., DUPRAT, F., HEURTEAUX, C., HUGNOT, J-P. AND LAZDUNSKI, M.
New modulatory alpha subunits for mammalian shab K+ channels.
J.BIOL.CHEM. 272(39) 24371-24379 (1997).
 
5. SHEPARD, A.R. AND RAE, J.L.
Electrically silent potassium channel subunits from human lens epithelium.
AM.J.PHYSIOL. 277 C412-C424 (1999).

Documentation
Potassium ion (K+) channels are a structurally diverse group of proteins
that facilitate the flow of K+ ions across cell membranes. They are
ubiquitous, being present in virtually all cell types. Activation of K+
channels tends to hyperpolarise cells, reducing the membrane's electrical
resistance, dampening nervous activity. In eukaryotic cells, K+ channels
are involved in neural signalling and generation of the cardiac rhythm, and
act as effectors in signal transduction pathways involving G protein-
coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
maintenance of ionic homeostasis [1].
   
Structurally, Kv channels belong to the subfamily of K+ channels whose
subunits contain 6 transmembrane (TM) domains: these are the voltage-
gated K+ (Kv) channels, the KCNQ channels, the EAG-like K+ channels and
3 kinds of Ca2+-activated K+ channels (BK, IK and SK) [2]. All K+
channels share a characteristic sequence feature: a TMxTVGYG motif
that resides between the 2 C-terminal membrane spanning helices, and
forms the K+-selective pore domain [1-2].
 
The Kv family can be divided into 4 subfamilies on the basis of sequence
similarity and function: Shaker (Kv1), Shab (Kv2), Shaw (Kv3) and Shal 
(Kv4). All consist of pore-forming alpha subunits that associate with 
different types of beta subunit. To form a functional K+ channel pore,
4 alpha subunits and 4 beta subunits are required. The alpha subunits
have 6 well-conserved TM domains, a pore loop region and variable length
N- and C-termini. TM domains 5/6 and the loop region form the K+ 
channel pore through which the K+ ions pass [3]. 
 
More recently, 4 new electrically-silent alpha subunits have been cloned:
Kv5 (KCNF), Kv6 (KCNG), Kv8 and Kv9 (KCNS). These subunits do not themselves
possess any functional activity, but appear to form heteromeric channels
with Kv2 subunits, and thus modulate Shab channel activity [4]. When highly
expressed, they inhibit channel activity, but at lower levels show more
specific modulatory actions. Coexpression of Kv9 subunits with Shab subunits
produces a shift in the voltage-dependence of channel inactivation toward
more negative potentials [4]. In addition, Shab channel deactivation and 
inactivation are slowed, and the single channel conductance is increased [5].
 
KV9CHANNEL is a 7-element fingerprint that provides a signature for the Kv9
potassium channel alpha subunits. The fingerprint was derived from an
initial alignment of 6 sequences: the motifs were drawn from conserved 
regions spanning virtually the full alignment length - motifs 1-5 reside
within the N-terminal cytoplasmic domain; motif 6 spans TM domain 1; and
motif 7 encodes TM domain 6. Two iterations on SPTR39_15f were required to
reach convergence, at which point a true set comprising 8 sequences was
identified.
Summary Information
8 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
78888888
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
O35173        O35174        O43651        O43652        
O54900 O88758 O88759 Q9TT17
Sequence Titles
O35173      K+ VOLTAGE-GATED CHANNEL, SUBFAMILY S, 1 (POTASSIUM CHANNEL ALPHA SUBUNIT) - Mus musculus (Mouse). 
O35174 K+ VOLTAGE-GATED CHANNEL, SUBFAMILY S, 2 (POTASSIUM CHANNEL ALPHA SUBUNIT) - Mus musculus (Mouse).
O43651 SHAB-RELATED DELAYED-RECTIFIER K+ CHANNEL ALPHA SUBUNIT - Homo sapiens (Human).
O43652 DELAYED-RECTIFIER K+ CHANNEL ALPHA SUBUNIT - Homo sapiens (Human).
O54900 SHAB-RELATED DELAYED-RECTIFIER K+ CHANNEL - Rattus norvegicus (Rat).
O88758 POTASSIUM CHANNEL, ALPHA SUBUNIT - Rattus norvegicus (Rat).
O88759 POTASSIUM CHANNEL, ALPHA SUBUNIT - Rattus norvegicus (Rat).
Q9TT17 VOLTAGE-GATED DELAYED-RECTIFIER POTASSIUM CHANNEL KV9.3 - Oryctolagus cuniculus (Rabbit).
Scan History
SPTR39_15f 2  8    NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
NVGGFKQSVDQST O43651 20 20 -
NVGGFKQSVDQST O54900 20 20 -
NVGGVRRQLSARA O43652 55 55 -
NVGGVRRLLSARA O88758 24 24 -
NVGGVRRLLSARA O35173 24 24 -
NVGGFKRRLRSHT O35174 22 22 -

Motif 2 width=17
Element Seqn Id St Int Rpt
RFPHTRLGKLLTCHSEE O43651 35 2 -
RFPHTRLGKLLTCHSEE O54900 35 2 -
RFPGTRLGRLQAAASEE O43652 70 2 -
RFPGTRLGRLQAAVSEE O88758 39 2 -
RFPGTRLGRLQAAASEE O35173 39 2 -
RFPETRLGRLLLCHSRE O35174 37 2 -

Motif 3 width=14
Element Seqn Id St Int Rpt
DDYSVADKEYYFDR O43651 58 6 -
DDYSVADKEYYFDR O54900 58 6 -
DDYDEAAREFYFDR O43652 93 6 -
DDYDAAAREFYFDR O88758 62 6 -
DDYDAAAHEFYFDR O35173 62 6 -
DDYDDVQREFYFDR O35174 60 6 -

Motif 4 width=16
Element Seqn Id St Int Rpt
FYYTGKLHVMEELCVF O43651 82 10 -
FYYTGKLHVMEELCVF O54900 82 10 -
FYRTGHLHVLDELCVF O43652 117 10 -
FYRTGHLHVLDELCVF O88758 86 10 -
FYRTGHLHVLDELCVF O35173 86 10 -
FYHTGKLHVMAELCVF O35174 84 10 -

Motif 5 width=13
Element Seqn Id St Int Rpt
YWGINELFIDSCC O43651 105 7 -
YWGINELFFDSCC O54900 105 7 -
YWGLGENALAACC O43652 140 7 -
YWGLGENALATCC O88758 109 7 -
YWGLGENALATCC O35173 109 7 -
YWGINEFFIDSCC O35174 107 7 -

Motif 6 width=13
Element Seqn Id St Int Rpt
SLSVVLASIVAMC O43651 191 73 -
SLSVVLASIVAMC O54900 191 73 -
SISVVLASIAAMC O43652 226 73 -
SIGVVLASIAAMC O88758 195 73 -
SIGVVLASIAAMC O35173 195 73 -
SILVVLGSIITMC O35174 193 73 -

Motif 7 width=19
Element Seqn Id St Int Rpt
CGILVVALPITIIFNKFSK O43651 393 189 -
CGILVVALPITIIFNKFSK O54900 393 189 -
GGILVVALPITIIFNKFSH O43652 444 205 -
GGILVVALPITIIFNKFSH O88758 415 207 -
GGILVVALPITIIFNKFSH O35173 415 207 -
AGILVVVLPITLIFNKFSH O35174 397 191 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
NVGGFKQSVDQST O43651 20 20 -
NVGGFKQSVDQST Q9TT17 20 20 -
NVGGFKQSVDQST O88759 20 20 -
NVGGFKQSVDQST O54900 20 20 -
NVGGVRRQLSARA O43652 55 55 -
NVGGVRRLLSARA O88758 24 24 -
NVGGVRRLLSARA O35173 24 24 -
NVGGFKRRLRSHT O35174 22 22 -

Motif 2 width=17
Element Seqn Id St Int Rpt
RFPHTRLGKLLTCHSEE O43651 35 2 -
RFPHTRLGKLLTCHSEE Q9TT17 35 2 -
RFPHTRLGKLLTCHSEE O88759 35 2 -
RFPHTRLGKLLTCHSEE O54900 35 2 -
RFPGTRLGRLQAAASEE O43652 70 2 -
RFPGTRLGRLQAAVSEE O88758 39 2 -
RFPGTRLGRLQAAASEE O35173 39 2 -
RFPETRLGRLLLCHSRE O35174 37 2 -

Motif 3 width=14
Element Seqn Id St Int Rpt
DDYSVADKEYYFDR O43651 58 6 -
DDYSVADKEYYFDR Q9TT17 58 6 -
DDYSVADKEYYFDR O88759 58 6 -
DDYSVADKEYYFDR O54900 58 6 -
DDYDEAAREFYFDR O43652 93 6 -
DDYDAAAREFYFDR O88758 62 6 -
DDYDAAAHEFYFDR O35173 62 6 -
DDYDDVQREFYFDR O35174 60 6 -

Motif 4 width=16
Element Seqn Id St Int Rpt
FYYTGKLHVMEELCVF O43651 82 10 -
FYYTGKLHVMEELCVF Q9TT17 82 10 -
FYYTGKLHVMEELCVF O88759 82 10 -
FYYTGKLHVMEELCVF O54900 82 10 -
FYRTGHLHVLDELCVF O43652 117 10 -
FYRTGHLHVLDELCVF O88758 86 10 -
FYRTGHLHVLDELCVF O35173 86 10 -
FYHTGKLHVMAELCVF O35174 84 10 -

Motif 5 width=13
Element Seqn Id St Int Rpt
YWGINELFIDSCC O43651 105 7 -
YWGINELFIDSCC Q9TT17 105 7 -
YWGINELFIDSCC O88759 105 7 -
YWGINELFFDSCC O54900 105 7 -
YWGLGENALAACC O43652 140 7 -
YWGLGENALATCC O88758 109 7 -
YWGLGENALATCC O35173 109 7 -
YWGINEFFIDSCC O35174 107 7 -

Motif 6 width=13
Element Seqn Id St Int Rpt
SLSVVLASIVAMC O43651 191 73 -
SLSVVLASIVAMC Q9TT17 191 73 -
SLSVVLASIVAMC O88759 191 73 -
SLSVVLASIVAMC O54900 191 73 -
SISVVLASIAAMC O43652 226 73 -
SIGVVLASIAAMC O88758 195 73 -
SIGVVLASIAAMC O35173 195 73 -
SILVVLGSIITMC O35174 193 73 -

Motif 7 width=19
Element Seqn Id St Int Rpt
CGILVVALPITIIFNKFSK O43651 393 189 -
CGILVVALPITIIFNKFSK Q9TT17 393 189 -
CGILVVALPITIIFNKFSK O88759 393 189 -
CGILVVALPITIIFNKFSK O54900 393 189 -
GGILVVALPITIIFNKFSH O43652 444 205 -
GGILVVALPITIIFNKFSH O88758 415 207 -
GGILVVALPITIIFNKFSH O35173 415 207 -
AGILVVVLPITLIFNKFSH O35174 397 191 -