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PR01421

Identifier
GPR37ORPHANR  [View Relations]  [View Alignment]  
Accession
PR01421
No. of Motifs
6
Creation Date
27-NOV-2000
Title
GPR37 orphan receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. MARAZZITI,D., GOLINI, E., GALLO,A., LOMBARDI., M.S., MATTEONI,
R. AND TOCCHINI-VALENTINI G.P.
Cloning of GPR37, a gene located on chromosome 7 encoding a
putative G protein-coupled peptide receptor, from a human frontal
brain EST library.
GENOMICS 45 68-77 (1997).
 
7. MARAZZITI, D., GALLO, A., GOLINI, E.,  MATTEONI, R. AND
TOCCHINI-VALENTINI G.P.
Molecular cloning and chromosomal localization of the mouse Gpr37
gene encoding an orphan G protein-coupled peptide receptor
expressed in brain and testis.
GENOMICS 53 315-324 (1998).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5].
 
Several 7TM receptors have been cloned but their endogenous ligands are
unknown; these have been termed orphan receptors. GPR37 was isolated from a
set of human brain frontal lobe expressed sequence tags. The GPR37 genomic
sequence was subsequently mapped to chromosome 7. A putative orthologue, 83%
identical to the human form in terms of predicted amino acid sequence, has
since been identified in the mouse genome and mapped to chromosome 6.
Northern blot analyses revealed a highly expressed 3.8kb mRNA and a less
abundant 8kb mRNA in both human and mouse brain. The 3.8kb mRNA was also
less abundantly expressed in human liver and placenta, and a further 3kb
mRNA was found in mouse testis [6,7].
 
GPR37ORPHANR is a 6-element fingerprint that provides a signature for the
GPR37 orphan receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within the C-terminal half of the alignment, focusing on those areas of the
alignment that characterise the GPR37 receptors but distinguish them from
the rest of the rhodopsin-like superfamily - motif 1 spans the first
external loop; motifs 2 and 3 span the second external loop, leading into
TM domain 5; motif 4 lies within TM domain 5; motif 5 lies at the C-terminus
of TM domain 5, leading into the third cytoplasmic loop; and motif 6 lies
at the C-terminus of TM domain 7. Two iterations on SPTR39_14f were required
to reach convergence, at which point a true set comprising 5 sequences was
identified.
Summary Information
9 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6999999
5000000
4000000
3000000
2000000
123456
True Positives
O00348        O14768        O15354        O60883        
O88313 Q9QY42 Q9QYC5 Q9QYC6
Q9Z0G3
Sequence Titles
O00348      PUTATIVE ENDOTHELIN RECEPTOR TYPE B-LIKE PROTEIN - Homo sapiens (Human). 
O14768 PUTATIVE G PROTEIN-COUPLED RECEPTOR (G PROTEIN COUPLED RECEPTOR 37) - Homo sapiens (Human).
O15354 G PROTEIN COUPLED RECEPTOR 37 - Homo sapiens (Human).
O60883 ETBR-LP-2 PROTEIN - Homo sapiens (Human).
O88313 G-PROTEIN COUPLED RECEPTOR - Mus musculus (Mouse).
Q9QY42 G PROTEIN-COUPLED RECEPTOR 37 - Mus musculus (Mouse).
Q9QYC5 G PROTEIN-COUPLED RECEPTOR GPCR/CNS2 - Rattus norvegicus (Rat).
Q9QYC6 G PROTEIN-COUPLED RECEPTOR GPCR/CNS1 - Rattus norvegicus (Rat).
Q9Z0G3 G PROTEIN-COUPLED PEPTIDE RECEPTOR - Mus musculus (Mouse).
Scan History
SPTR39_14f 2  300  NSINGLE    
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
VIFHELTKKWLLEDFSCKIVP Q9QY42 305 305 -
VIFHELTKKWLLEDFSCKIVP O15354 318 318 -
VIFNEITKQRLLGDVSCRAVP O88313 54 54 -

Motif 2 width=14
Element Seqn Id St Int Rpt
RQLSKEDLGFSGQA Q9QY42 388 62 -
RQLSKEDLGFSGRA O15354 401 62 -
WQLAQEPAPTAGTV O88313 137 62 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ERCVIKISPDLPDTIY Q9QY42 404 2 -
ERCIIKISPDLPDTIY O15354 417 2 -
DSCIMKPSADLPESVY O88313 151 0 -

Motif 4 width=14
Element Seqn Id St Int Rpt
ARLWWYFGCYFCLP Q9QY42 428 8 -
ARLWWYFGCYFCLP O15354 441 8 -
ARMWWYFGCYFCLP O88313 175 8 -

Motif 5 width=11
Element Seqn Id St Int Rpt
LFTITCSLVTA Q9QY42 443 1 -
LFTITCSLVTA O15354 456 1 -
LFTVTCQLVTW O88313 190 1 -

Motif 6 width=12
Element Seqn Id St Int Rpt
VTPVLLFCLCRP Q9QY42 531 77 -
VTPVLLFCLCKP O15354 544 77 -
ITPVLLLCICRP O88313 278 77 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
VIFHELTKKWLLEDFSCKIVP Q9QY42 305 305 -
VIFHELTKKWLLEDFSCKIVP Q9QYC6 308 308 -
VIFHELTKKWLLEDFSCKIVP Q9Z0G3 305 305 -
VIFHELTKKWLLEDFSCKIVP O14768 318 318 -
VIFHELTKKWLLEDFSCKIVP O15354 318 318 -
VIFHELTKKWLVEDFSCKIVP O00348 318 318 -
VIFNEITKQRLLGDVSCRAVP Q9QYC5 187 187 -
VIFNEITKQRLLGDVSCRAVP O88313 54 54 -
VIFNEITKQRLLGDVSCRAVP O60883 187 187 -

Motif 2 width=14
Element Seqn Id St Int Rpt
RQLSKEDLGFSGQA Q9QY42 388 62 -
RQLSKEDLGFSGQA Q9QYC6 391 62 -
RQLSKEDLGFSGQA Q9Z0G3 388 62 -
RQLSKEDLGFSGRA O14768 401 62 -
RQLSKEDLGFSGRA O15354 401 62 -
RQLSKEDLGFSGRA O00348 401 62 -
WQLAQEPTPTMGTV Q9QYC5 270 62 -
WQLAQEPAPTAGTV O88313 137 62 -
WQLAQEPAPTMGTL O60883 270 62 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ERCVIKISPDLPDTIY Q9QY42 404 2 -
ERCVIKISPDLPDTIY Q9QYC6 407 2 -
ERCVIKISPDLPDTIY Q9Z0G3 404 2 -
ERCIIKISPDLPDTIY O14768 417 2 -
ERCIIKISPDLPDTIY O15354 417 2 -
ERCIIKISPDLPDTIY O00348 417 2 -
DSCIMKPSADLPESLY Q9QYC5 284 0 -
DSCIMKPSADLPESVY O88313 151 0 -
DSCIMKPSASLPESLY O60883 284 0 -

Motif 4 width=14
Element Seqn Id St Int Rpt
ARLWWYFGCYFCLP Q9QY42 428 8 -
ARLWWYFGCYFCLP Q9QYC6 431 8 -
ARLWWYFGCYFCLP Q9Z0G3 428 8 -
ARLWWYFGCYFCLP O14768 441 8 -
ARLWWYFGCYFCLP O15354 441 8 -
ARLWWYFGCYFCLP O00348 441 8 -
ARMWWYFGCYFCLP Q9QYC5 308 8 -
ARMWWYFGCYFCLP O88313 175 8 -
ARMWWYFGCYFCLP O60883 308 8 -

Motif 5 width=11
Element Seqn Id St Int Rpt
LFTITCSLVTA Q9QY42 443 1 -
LFTITCSLVTA Q9QYC6 446 1 -
LFTITCSLVTA Q9Z0G3 443 1 -
LFTITCSLVTA O14768 456 1 -
LFTITCSLVTA O15354 456 1 -
LFTITCSLVTA O00348 456 1 -
LFTVTCQLVTW Q9QYC5 323 1 -
LFTVTCQLVTW O88313 190 1 -
LFTVTCQLVTW O60883 323 1 -

Motif 6 width=12
Element Seqn Id St Int Rpt
VTPVLLFCLCRP Q9QY42 531 77 -
VTPVLLFCLCRP Q9QYC6 534 77 -
VTPVLLFCLCRP Q9Z0G3 531 77 -
VTPVLLFCLCKP O14768 544 77 -
VTPVLLFCLCKP O15354 544 77 -
VTPVLLFCLCKP O00348 544 77 -
ITPVLLLCICRP Q9QYC5 411 77 -
ITPVLLLCICRP O88313 278 77 -
ITPVLLLCICRP O60883 411 77 -