SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01417

Identifier
GHSRECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01417
No. of Motifs
10
Creation Date
23-SEP-2000
Title
Growth hormone secretagogue receptor type 1 signature 
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN

GCRDB; GCR_1383; GCR_1917; GCR_1918; GCR_1532; GCR_1539
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. MCKEE, K.K., PALYHA, O.C., FEIGHNER, S.D., HRENIUK, D.L., TAN, C.P.,
PHILLIPS, M.S., SMITH, R.G., VAN DER PLOEG, L.H. AND HOWARD, A.D.
Molecular analysis of rat pituitary and hypothalamic growth hormone 
secretagogue receptors.
MOL.ENDOCRINOL. 11 415-423 (1997). 
 
7. HOWARD, A.D., FEIGHNER, S.D., CULLY, D.F., ARENA, J.P., LIBERATOR, P.A.,
ROSENBLUM, C.I., HAMELIN, M., HRENIUK, D.L., PALYHA, O.C., ANDERSON, J.,
PARESS, P.S., DIAZ, C., CHOU, M., LIU, K.K., MCKEE, K.K., PONG, S.-S., 
CHAUNG, L.-Y., ELBRECHT, A., DASHKEVICZ, M., HEAVENS, R., RIGBY, M.,
SIRINATHSINGHJI, D.J.S., DEAN, D.C., MELILLO, D.G., PATCHETT, A.A., 
NARGUND, R., GRIFFIN, P.R., DEMARTINO, J.A., GUPTA, S.K., SCHAEFFER, J.M.,
SMITH, R.G. AND VAN DER PLOEG, L.H.T.
A receptor in pituitary and hypothalamus that functions in growth hormone
release.
SCIENCE 273 974-977 (1996).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Growth hormone (GH) release is thought to occur under the reciprocal
regulation of two hypothalamic peptides (GH releasing hormone (GHRH) and
somatostatin) via their engagement with specific cell surface receptors on
the anterior pituitary somatotroph [6,7]. GH-releasing peptides (such as
GHRP-6, and the nonpeptide mimetics, L-692, 429 and MK-0677) stimulate GH 
release through their activation of a distinct receptor, the GH secretagogue
receptor (GHSR). This receptor couples to G-alpha-11 proteins.  
 
GHSRECEPTOR is a 10-element fingerprint that provides a signature for the
growth hormone secretagogue type 1 receptors. The fingerprint was derived
from an initial alignment of 3 sequences: the motifs were drawn from 
conserved sections within either loop or TM regions, focusing on those 
areas of the alignment that characterise the growth hormone secretagogue 
type 1 receptors but distinguish them from the rest of the rhodopsin-like
superfamily - motifs 1-3 span the N-terminal region, leading into TM domain
1;  motif 4 encompasses part of the first external loop and TM domain 3; 
motif 5 spans part of the second cytoplasmic loop and the N-terminal portion
of TM domain 4; motif 6 lies in the second cytoplasmic loop; motif 7
includes part of the second external loop and the N-terminus of TM domain 5;
motifs 8 and 9 spans the third cytoplasmic and third external loops 
respectively; and motif 10 resides at the C-terminus. A single iteration on
SPTR37_10f was required to reach convergence, no further sequences being
identified beyond the starting set. Two partial matches were found: O93412
is an orphan GPCR from Sphoeroides nephelus that matches motifs 4, 7 and 9;
and GP38_HUMAN is a putative GPCR from Homo sapiens that matches motifs 3
and 4.   
Summary Information
   3 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
103333333333
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
30001001010
20011000000
12345678910
True Positives
GHSR_HUMAN    GHSR_PIG      GHSR_RAT      
True Positive Partials
Codes involving 3 elements
O93412
Codes involving 2 elements
GP38_HUMAN
Sequence Titles
GHSR_HUMAN  Growth hormone secretagogue receptor type 1 (GHS-R) (GH-releasing peptide receptor) (GHRP) (Ghrelin receptor) - Homo sapiens (Human). 
GHSR_PIG Growth hormone secretagogue receptor type 1 (GHS-R) (GH-releasing peptide receptor) (GHRP) (Ghrelin receptor) - Sus scrofa (Pig).
GHSR_RAT Growth hormone secretagogue receptor type 1 (GHS-R) (GH-releasing peptide receptor) (GHRP) (Ghrelin receptor) - Rattus norvegicus (Rat).

O93412 ORPHAN G PROTEIN-COUPLED RECEPTOR - SPHOEROIDES NEPHELUS.

GP38_HUMAN Putative G protein-coupled receptor GPR38 - Homo sapiens (Human).
Scan History
SPTR37_10f 1  40   NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
WNATPSEEPEPNVTL GHSR_RAT 2 2 -
WNATPSEEPGFNLTL GHSR_HUMAN 2 2 -
WNATPSEEPGPNLTL GHSR_PIG 2 2 -

Motif 2 width=18
Element Seqn Id St Int Rpt
DLDWDASPGNDSLPDELL GHSR_RAT 17 0 -
DLDWDASPGNDSLGDELL GHSR_HUMAN 18 1 -
DLGWDAPPENDSLVEELL GHSR_PIG 18 1 -

Motif 3 width=18
Element Seqn Id St Int Rpt
LFPAPLLAGVTATCVALF GHSR_RAT 36 1 -
LFPAPLLAGVTATCVALF GHSR_HUMAN 37 1 -
LFPTPLLAGVTATCVALF GHSR_PIG 37 1 -

Motif 4 width=17
Element Seqn Id St Int Rpt
FQFVSESCTYATVLTIT GHSR_RAT 118 64 -
FQFVSESCTYATVLTIT GHSR_HUMAN 119 64 -
FQFVSESCTYATVLTIT GHSR_PIG 119 64 -

Motif 5 width=14
Element Seqn Id St Int Rpt
AKVVVTKGRVKLVI GHSR_RAT 150 15 -
AKVVVTKGRVKLVI GHSR_HUMAN 151 15 -
AKVVVTKGRVKLVI GHSR_PIG 151 15 -

Motif 6 width=15
Element Seqn Id St Int Rpt
GVEHENGTDPRDTNE GHSR_RAT 182 18 -
GVEHENGTDPWDTNE GHSR_HUMAN 183 18 -
GVEHDNGTDPRDTNE GHSR_PIG 183 18 -

Motif 7 width=15
Element Seqn Id St Int Rpt
TEFAVRSGLLTVMVW GHSR_RAT 200 3 -
TEFAVRSGLLTVMVW GHSR_HUMAN 201 3 -
TEFAVRSGLLTVMVW GHSR_PIG 201 3 -

Motif 8 width=20
Element Seqn Id St Int Rpt
RGDAAVGASLRDQNHKQTVK GHSR_RAT 242 27 -
RGDAVVGASLRDQNHKQTVK GHSR_HUMAN 244 28 -
RGEAAVGSSLRDQNHKQTVK GHSR_PIG 244 28 -

Motif 9 width=21
Element Seqn Id St Int Rpt
FHVGRYLFSKSFEPGSLEIAQ GHSR_RAT 277 15 -
FHVGRYLFSKSFEPGSLEIAQ GHSR_HUMAN 279 15 -
FHVGRYLFSKSLEPGSVEIAQ GHSR_PIG 279 15 -

Motif 10 width=23
Element Seqn Id St Int Rpt
FSQRKLSTLKDESSRAWTKSSIN GHSR_RAT 341 43 -
FSQRKLSTLKDESSRAWTESSIN GHSR_HUMAN 343 43 -
FSQRKLSTLKDESSRAWTESSIN GHSR_PIG 343 43 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
WNATPSEEPEPNVTL GHSR_RAT 2 2 -
WNATPSEEPGFNLTL GHSR_HUMAN 2 2 -
WNATPSEEPGPNLTL GHSR_PIG 2 2 -

Motif 2 width=18
Element Seqn Id St Int Rpt
DLDWDASPGNDSLPDELL GHSR_RAT 17 0 -
DLDWDASPGNDSLGDELL GHSR_HUMAN 18 1 -
DLGWDAPPENDSLVEELL GHSR_PIG 18 1 -

Motif 3 width=18
Element Seqn Id St Int Rpt
LFPAPLLAGVTATCVALF GHSR_RAT 36 1 -
LFPAPLLAGVTATCVALF GHSR_HUMAN 37 1 -
LFPTPLLAGVTATCVALF GHSR_PIG 37 1 -

Motif 4 width=17
Element Seqn Id St Int Rpt
FQFVSESCTYATVLTIT GHSR_RAT 118 64 -
FQFVSESCTYATVLTIT GHSR_HUMAN 119 64 -
FQFVSESCTYATVLTIT GHSR_PIG 119 64 -

Motif 5 width=14
Element Seqn Id St Int Rpt
AKVVVTKGRVKLVI GHSR_RAT 150 15 -
AKVVVTKGRVKLVI GHSR_HUMAN 151 15 -
AKVVVTKGRVKLVI GHSR_PIG 151 15 -

Motif 6 width=15
Element Seqn Id St Int Rpt
GVEHENGTDPRDTNE GHSR_RAT 182 18 -
GVEHENGTDPWDTNE GHSR_HUMAN 183 18 -
GVEHDNGTDPRDTNE GHSR_PIG 183 18 -

Motif 7 width=15
Element Seqn Id St Int Rpt
TEFAVRSGLLTVMVW GHSR_RAT 200 3 -
TEFAVRSGLLTVMVW GHSR_HUMAN 201 3 -
TEFAVRSGLLTVMVW GHSR_PIG 201 3 -

Motif 8 width=20
Element Seqn Id St Int Rpt
RGDAAVGASLRDQNHKQTVK GHSR_RAT 242 27 -
RGDAVVGASLRDQNHKQTVK GHSR_HUMAN 244 28 -
RGEAAVGSSLRDQNHKQTVK GHSR_PIG 244 28 -

Motif 9 width=21
Element Seqn Id St Int Rpt
FHVGRYLFSKSFEPGSLEIAQ GHSR_RAT 277 15 -
FHVGRYLFSKSFEPGSLEIAQ GHSR_HUMAN 279 15 -
FHVGRYLFSKSLEPGSVEIAQ GHSR_PIG 279 15 -

Motif 10 width=23
Element Seqn Id St Int Rpt
FSQRKLSTLKDESSRAWTKSSIN GHSR_RAT 341 43 -
FSQRKLSTLKDESSRAWTESSIN GHSR_HUMAN 343 43 -
FSQRKLSTLKDESSRAWTESSIN GHSR_PIG 343 43 -