SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01403

Identifier
8OXTPHPHTASE  [View Relations]  [View Alignment]  
Accession
PR01403
No. of Motifs
7
Creation Date
30-APR-2000
Title
7,8-dihydro-8-oxoguanine triphosphatase signature
Database References
PRINTS; PR00502 NUDIXFAMILY
PROSITE; PS00893 ; MUTT
BLOCKS; BL00893
PFAM; PF00293 mutT
INTERPRO; IPR000086
Literature References
1. CAI, J.P., KAKUMA, T., TSUZUKI, T. AND SEKIGUCHI, M.
cDNA and genomic sequences for rat 8-oxo-dGTPase that prevents occurrence
of spontaneous mutations due to oxidation of guanine nucleotides.
CARCINOGENESIS 16 2343-2350 (1995). 
 
2. SAKUMI, K., FURUICHI, M., TSUZUKI, T., KAKUMA, T., KAWABATA, S., 
MAKI, H. AND SEKIGUCHI, M.
Cloning and expression of cDNA for a human enzyme that hydrolyzes 
8-oxo-dGTP, a mutagenic substrate for DNA synthesis.
J.BIOL.CHEM. 268 23524-23530 (1993).

Documentation
The enzyme 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo-dGTPase)
is present in a variety of organisms, where it plays an important role in
the control of spontaneous mutagenesis [1]. The enzyme degrades 8-oxoguanine-
containing deoxyribonucleoside triphosphate, a potentially mutagenic
substrate for DNA synthesis, to the corresponding monophosphate, thereby
preventing misincorporation of 8-oxo-dGTP into DNA [1]. 
 
8-oxo-dGTPase is an 18kDa protein containing 156 amino acid residues. The
amino acid sequence shares a degree of similarity with the MutT domain of 
the Escherichia coli MutT protein, which has a distinct 8-oxo-dGTPase 
activity [2]. When human cDNA is expressed in E.coli mutT- mutant cells,
there is a significant amount of 8-oxo-dGTPase activity and a reduced 
frequency of spontaneous mutation [2]. Human 8-oxo-dGTPase is thought to 
protect genetic information from the untoward effects of endogenous oxygen
radicals [2]. 
 
8OXTPHPHTASE is a 7-element fingerprint that provides a signature for 
7,8-dihydro-8-oxoguanine triphosphatases. The fingerprint was derived from
an initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning the N- and C-terminal portions of the alignment - motifs 1
and 2 lie N-terminal to the MutT domain; and motifs 3-7 lie C-terminal to
it. A single iteration on SPTR37_10f was required to reach convergence, no
further sequences being identified beyond the starting set.
Summary Information
3 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
73333333
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
8ODP_HUMAN    8ODP_MOUSE    8ODP_RAT      
Sequence Titles
8ODP_HUMAN  7,8-dihydro-8-oxoguanine triphosphatase - Homo sapiens (Human). 
8ODP_MOUSE 7,8-dihydro-8-oxoguanine triphosphatase - Mus musculus (Mouse).
8ODP_RAT 7,8-dihydro-8-oxoguanine triphosphatase - Rattus norvegicus (Rat).
Scan History
SPTR37_10f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
SRLYTLVLVLQPQRVLLG 8ODP_RAT 4 4 -
SRLYTLVLVLQPQRVLLG 8ODP_MOUSE 4 4 -
SRLYTLVLVLQPQRVLLG 8ODP_HUMAN 4 4 -

Motif 2 width=17
Element Seqn Id St Int Rpt
GMKKRGFGAGRWNGFGG 8ODP_RAT 21 -1 -
GMKKRGFGAGRWNGFGG 8ODP_MOUSE 21 -1 -
GMKKRGFGAGRWNGFGG 8ODP_HUMAN 21 -1 -

Motif 3 width=22
Element Seqn Id St Int Rpt
VDTLHKVGHISFEFVGSPELMD 8ODP_RAT 61 23 -
VDTLHKVGHISFEFVGSPELMD 8ODP_MOUSE 61 23 -
VDALHKVGQIVFEFVGEPELMD 8ODP_HUMAN 61 23 -

Motif 4 width=20
Element Seqn Id St Int Rpt
DVHIFSTDHVHGTPTESEEM 8ODP_RAT 82 -1 -
DVHIFSADHVHGTPTESEEM 8ODP_MOUSE 82 -1 -
DVHVFCTDSIQGTPVESDEM 8ODP_HUMAN 82 -1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
RPQWFQLDQIPFADMWPDD 8ODP_RAT 102 0 -
RPQWFQLDQIPFADLWPDD 8ODP_MOUSE 102 0 -
RPCWFQLDQIPFKDMWPDD 8ODP_HUMAN 102 0 -

Motif 6 width=19
Element Seqn Id St Int Rpt
SYWFPLLLQKKKFCGHFKF 8ODP_RAT 121 0 -
SYWFPLLLQKKKFCGHFKF 8ODP_MOUSE 121 0 -
SYWFPLLLQKKKFHGYFKF 8ODP_HUMAN 121 0 -

Motif 7 width=16
Element Seqn Id St Int Rpt
FHGQDTILSYSLREVD 8ODP_RAT 139 -1 -
FQDQDTILSYSLREVD 8ODP_MOUSE 139 -1 -
FQGQDTILDYTLREVD 8ODP_HUMAN 139 -1 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
SRLYTLVLVLQPQRVLLG 8ODP_RAT 4 4 -
SRLYTLVLVLQPQRVLLG 8ODP_MOUSE 4 4 -
SRLYTLVLVLQPQRVLLG 8ODP_HUMAN 4 4 -

Motif 2 width=17
Element Seqn Id St Int Rpt
GMKKRGFGAGRWNGFGG 8ODP_RAT 21 -1 -
GMKKRGFGAGRWNGFGG 8ODP_MOUSE 21 -1 -
GMKKRGFGAGRWNGFGG 8ODP_HUMAN 21 -1 -

Motif 3 width=22
Element Seqn Id St Int Rpt
VDTLHKVGHISFEFVGSPELMD 8ODP_RAT 61 23 -
VDTLHKVGHISFEFVGSPELMD 8ODP_MOUSE 61 23 -
VDALHKVGQIVFEFVGEPELMD 8ODP_HUMAN 61 23 -

Motif 4 width=20
Element Seqn Id St Int Rpt
DVHIFSTDHVHGTPTESEEM 8ODP_RAT 82 -1 -
DVHIFSADHVHGTPTESEEM 8ODP_MOUSE 82 -1 -
DVHVFCTDSIQGTPVESDEM 8ODP_HUMAN 82 -1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
RPQWFQLDQIPFADMWPDD 8ODP_RAT 102 0 -
RPQWFQLDQIPFADLWPDD 8ODP_MOUSE 102 0 -
RPCWFQLDQIPFKDMWPDD 8ODP_HUMAN 102 0 -

Motif 6 width=19
Element Seqn Id St Int Rpt
SYWFPLLLQKKKFCGHFKF 8ODP_RAT 121 0 -
SYWFPLLLQKKKFCGHFKF 8ODP_MOUSE 121 0 -
SYWFPLLLQKKKFHGYFKF 8ODP_HUMAN 121 0 -

Motif 7 width=16
Element Seqn Id St Int Rpt
FHGQDTILSYSLREVD 8ODP_RAT 139 -1 -
FQDQDTILSYSLREVD 8ODP_MOUSE 139 -1 -
FQGQDTILDYTLREVD 8ODP_HUMAN 139 -1 -