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PR01400

Identifier
TACYTOLYSIN  [View Relations]  [View Alignment]  
Accession
PR01400
No. of Motifs
11
Creation Date
01-JUN-2000
Title
Bacterial thiol-activated pore-forming cytolysin signature
Database References

PROSITE; PS00481 THIOL_CYTOLYSINS
BLOCKS; BL00481
PFAM; PF01289 Thiol_cytolysin
INTERPRO; IPR001869
PDB; 1PFO
SCOP; 1PFO
CATH; 1PFO
Literature References
1. KEHOE, M.A., MILLER, L., WALKER, J.A. AND BOULNOIS, G.J.
Nucleotide sequence of the Streptolysin O (SLO) gene: structural homologies
between SLO and other membrane-damaging thiol-activated toxins.
INFECT.IMMUN. 55 3228-3232 (1987).
 
2. TWETEN, R.K.
Nucleotide sequence of the gene for Perfringolysin O (Theta-toxin) from
Clostridium perfringens: significant homology with the genes for 
streptolysin O and pneumolysin.
INFECT.IMMUN. 56 3235-3240 (1988).
 
3. MENGAUD, J., CHENEVERT, J., GEOFFROY, C., GAILLARD, J.L. AND COSSART P.
Identification of the structural gene encoding the SH-activated hemolysin 
of Listeria monocytogenes: Listeriolysin O is homologous to Streptolysin O
and Pneumolysin.
INFECT.IMMUN. 55 3225-3227 (1987).
 
4. ROSSJOHN, J., FEIL, S.C., MCKINSTRY, W.J., TWETEN, R.K. AND PARKER, M.W.
Structure of a cholesterol-binding, thiol-activated cytolysin and a
model of its membrane form.
CELL 89 685-692 (1997).

Documentation
One method of pathogenicity employed by infectious bacteria is the formation 
of pores through the host cell membranes [1]. This in turn can cause solute/
protein loss, osmotic cell lysis, and cell death. The pathogen achieves this
by the secretion of exotoxin monomers, which assemble in the host membrane 
to form hydrophilic channels [1].
 
Thiol-activated cytolysins are lytic for a wide range of eukaryotic cells, 
and are reversibly sensitive to oxidation, due to their activation by 
thiols [1]. Cholesterol is bound by these moieties when assembling in the 
target membrane, and is believed to be the receptor for such toxins. 
 
Members of the cytolysin family include the streptolysin O toxin of
Streptococcal species [1], Clostridium perfringens perfringolysin O [2], 
and the exotoxin listeriolysin from L.monocytogenes [3]. All contain a
conserved cysteine, and share structural similarity in their C-termini [3].
Perhaps the most well studied mechanism is that of perfringolysin O, which
binds to cholesterol in the membrane, and inserts via a novel TM amphipathic
hairpin arrangement [2].
 
The structure of perfringolysin o from Clostridium perfringens has been 
determined to 2.2A resolution using X-ray crystallography [4]. The protein
contains 2 domains, the first of which has an alpha+beta fold, while
the second has an all-beta fold.
 
TACYTOLYSIN is an 11-element fingerprint that provides a signature for 
bacterial thiol-activated pore-forming cytolysins. The fingerprint was
derived from an initial alignment of 4 sequences: the motifs were drawn
from conserved regions spanning the full alignment length - motif 1 includes
beta-strand 1; motif 2 spans strands 3 and 4; motif 3 spans strand 5 and 
alpha-helix 2; motif 4 includes strand 6; motif 5 spans strand 9 and the
N-terminal portion of helix 4; motif 6 encodes strand 11; motif 7 spans
the C-terminal half of helix 11 and strand 12; motif 8 spans strand 15 and
the N-terminal portion of strand 16; motifs 9, 10 and 11 encode strands 18,
19 and 23 respectively. Two iterations on SPTR37_10f were required to reach
convergence, at which point a true set comprising 12 sequences was 
identified.
Summary Information
12 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
111212121212121212121212
1000000000000
900000000000
800000000000
700000000000
600000000000
500000000000
400000000000
300000000000
200000000000
1234567891011
True Positives
O31241        O85102        Q55996        TACY_CLOPE    
TACY_LISIV TACY_LISMO TACY_LISSE TACY_PAEAL
TACY_STRCB TACY_STREQ TACY_STRPN TACY_STRPY
Sequence Titles
O31241      PYOLYSIN - ARCANOBACTERIUM PYOGENES.          
O85102 HEMOLYSIN - STREPTOCOCCUS SUIS.
Q55996 SUILYSIN - STREPTOCOCCUS SUIS.
TACY_CLOPE PERFRINGOLYSIN O PRECURSOR (THETA-TOXIN) (THIOL-ACTIVATED CYTOLYSIN) - CLOSTRIDIUM PERFRINGENS.
TACY_LISIV IVANOLYSIN PRECURSOR (THIOL-ACTIVATED CYTOLYSIN) - LISTERIA IVANOVII.
TACY_LISMO LISTERIOLYSIN O PRECURSOR (THIOL-ACTIVATED CYTOLYSIN) - LISTERIA MONOCYTOGENES.
TACY_LISSE SEELIGERIOLYSIN PRECURSOR (THIOL-ACTIVATED CYTOLYSIN) - LISTERIA SEELIGERI.
TACY_PAEAL ALVEOLYSIN PRECURSOR (THIOL-ACTIVATED CYTOLYSIN) - PAENIBACILLUS ALVEI (BACILLUS ALVEI).
TACY_STRCB STREPTOLYSIN O PRECURSOR (THIOL-ACTIVATED CYTOLYSIN) - STREPTOCOCCUS CANIS.
TACY_STREQ STREPTOLYSIN O PRECURSOR (THIOL-ACTIVATED CYTOLYSIN) - STREPTOCOCCUS EQUISIMILIS.
TACY_STRPN PNEUMOLYSIN (THIOL-ACTIVATED CYTOLYSIN) - STREPTOCOCCUS PNEUMONIAE.
TACY_STRPY STREPTOLYSIN O PRECURSOR (THIOL-ACTIVATED CYTOLYSIN) - STREPTOCOCCUS PYOGENES.
Scan History
SPTR37_10f 2  50   NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
LNYNELEVLAKNGETIEN TACY_STRPY 116 116 -
LSYNRNEVLASNGDKIES TACY_CLOPE 45 45 -
LDYNKNNVLVYHGDAVTN TACY_LISMO 68 68 -
LTYEPQEILTNEGEYIDN Q55996 40 40 -

Motif 2 width=23
Element Seqn Id St Int Rpt
FIVIERKKKNINTTPVDISIIDS TACY_STRPY 146 12 -
FIVVERQKRSLTTSPVDISIIDS TACY_CLOPE 75 12 -
YIVVEKKKKSINQNNADIQVVNA TACY_LISMO 98 12 -
FVVLRREKKNITNNSADIAVIDA Q55996 70 12 -

Motif 3 width=17
Element Seqn Id St Int Rpt
YPAALQLANKGFTENKP TACY_STRPY 174 5 -
YPGALQLADKAFVENRP TACY_CLOPE 103 5 -
YPGALVKANSELVENQP TACY_LISMO 126 5 -
YPGALLRADQNLLDNNP Q55996 98 5 -

Motif 4 width=19
Element Seqn Id St Int Rpt
PDAVVTKRNPQKIHIDLPG TACY_STRPY 190 -1 -
PTILMVKRKPININIDLPG TACY_CLOPE 119 -1 -
PDVLPVKRDSLTLSIDLPG TACY_LISMO 142 -1 -
PTLISIARGDLTLSLNLPG Q55996 114 -1 -

Motif 5 width=16
Element Seqn Id St Int Rpt
ARTQYTESMVYSKSQI TACY_STRPY 248 39 -
ARTQYSESMVYSKSQI TACY_CLOPE 177 39 -
AKIDYDDEMAYSESQL TACY_LISMO 202 41 -
AELQYDETMAYSMSQL Q55996 174 41 -

Motif 6 width=24
Element Seqn Id St Int Rpt
GVSNEAPPLFVSNVAYGRTVFVKL TACY_STRPY 329 65 -
GVSNEAPPLMVSNVAYGRTIYVKL TACY_CLOPE 258 65 -
GVNAENPPAYISSVAYGRQVYLKL TACY_LISMO 283 65 -
GITDEVPPVYVSSVSYGRSMFIKL Q55996 255 65 -

Motif 7 width=18
Element Seqn Id St Int Rpt
DILENSSFTAVVLGGDAA TACY_STRPY 382 29 -
DIYENSSFTAVVLGGDAQ TACY_CLOPE 311 29 -
NIIKNSSFKAVIYGGSAK TACY_LISMO 336 29 -
DILKNTSFSAYIFGGDAG Q55996 308 29 -

Motif 8 width=28
Element Seqn Id St Int Rpt
PISYTSVFLKNNKIAGVNNRTEYVETTS TACY_STRPY 430 30 -
PISYTSVFLKDNSVAAVHNKTDYIETTS TACY_CLOPE 359 30 -
PIAYTTNFLKDNELAVIKNNSEYIETTS TACY_LISMO 384 30 -
PISYSTNFVKDNRPAQILSNSEYIETTS Q55996 356 30 -

Motif 9 width=18
Element Seqn Id St Int Rpt
HQGAYVAQYEILWDEINY TACY_STRPY 469 11 -
HSGAYVAQFEVAWDEVSY TACY_CLOPE 398 11 -
HSGGYVAQFNISWDEVNY TACY_LISMO 423 11 -
HSGAYVAKYNITWEEVSY Q55996 395 11 -

Motif 10 width=17
Element Seqn Id St Int Rpt
YDDKGKEVITKRRWDNN TACY_STRPY 486 -1 -
YDKEGNEVLTHKTWDGN TACY_CLOPE 415 -1 -
YDPEGNEIVQHKNWSEN TACY_LISMO 440 -1 -
YNEAGEEVWEPKAWDKN Q55996 412 -1 -

Motif 11 width=21
Element Seqn Id St Int Rpt
ERDVKLSKEINVNISGSTLSP TACY_STRPY 544 41 -
EYDVPLTNNINVSIWGTTLYP TACY_CLOPE 473 41 -
DRNLPLVKNRNISIWGTTLYP TACY_LISMO 498 41 -
DKDLPLVGQRKITIWGTTLYP Q55996 469 40 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
LNYNELEVLAKNGETIEN TACY_STREQ 119 119 -
LNYNELEVLAKNGETIEN TACY_STRPY 116 116 -
LNYNELEVLAKNGETIEN TACY_STRCB 119 119 -
LSYNRNEVLASNGDKIES TACY_CLOPE 45 45 -
LNYDKNSILVYQGEAVTN TACY_LISSE 69 69 -
LDYDKNNILVYDGEAVKN TACY_LISIV 67 67 -
LDYNKNNVLVYHGDAVTN TACY_LISMO 68 68 -
LNYNRNEVLAIQGDQISS TACY_PAEAL 47 47 -
LTYGPQEILTNEGEYIDN O85102 40 40 -
LTYEPQEILTNEGEYIDN Q55996 40 40 -
MNYDKKKLLTHQGESIEN TACY_STRPN 12 12 -
LKYDPSGVLAVKGESIEN O31241 73 73 -

Motif 2 width=23
Element Seqn Id St Int Rpt
FIVIERKKKNINTTPVDISIIDS TACY_STREQ 149 12 -
FIVIERKKKNINTTPVDISIIDS TACY_STRPY 146 12 -
FIVIERKKKNINTTPVDISIIDS TACY_STRCB 149 12 -
FIVVERQKRSLTTSPVDISIIDS TACY_CLOPE 75 12 -
YIVVEKKKKGINQNNADISVINA TACY_LISSE 99 12 -
YIVVEKKKKSINQNNADIQVINS TACY_LISIV 97 12 -
YIVVEKKKKSINQNNADIQVVNA TACY_LISMO 98 12 -
FIVVERDKKSLTTSPVDISIVDS TACY_PAEAL 77 12 -
FVVLRREKKNITNNSADIAVIDA O85102 70 12 -
FVVLRREKKNITNNSADIAVIDA Q55996 70 12 -
FVVIERKKRSLSTNTSDISVTAT TACY_STRPN 42 12 -
YTVFKHERKSFNNLRSDISAFDA O31241 103 12 -

Motif 3 width=17
Element Seqn Id St Int Rpt
YPAALQLANKGFTENKP TACY_STREQ 177 5 -
YPAALQLANKGFTENKP TACY_STRPY 174 5 -
YPAALQLANKGFTENKP TACY_STRCB 177 5 -
YPGALQLADKAFVENRP TACY_CLOPE 103 5 -
YPGALVKANRELVENQP TACY_LISSE 127 5 -
YPGALVKANSELVENQP TACY_LISIV 125 5 -
YPGALVKANSELVENQP TACY_LISMO 126 5 -
YPGAIQLANKDFADNQP TACY_PAEAL 105 5 -
YPGALLRADQNLLDNNP O85102 98 5 -
YPGALLRADQNLLDNNP Q55996 98 5 -
YPGALLVVDETLLENNP TACY_STRPN 70 5 -
YPGALVLANKDLAKGSP O31241 131 5 -

Motif 4 width=19
Element Seqn Id St Int Rpt
PDAVVTKRNPQKIHIDLPG TACY_STREQ 193 -1 -
PDAVVTKRNPQKIHIDLPG TACY_STRPY 190 -1 -
PDAVVTKRNPQKIHIDLPG TACY_STRCB 193 -1 -
PTILMVKRKPININIDLPG TACY_CLOPE 119 -1 -
PNVLPVKRDSLTLSVDLPG TACY_LISSE 143 -1 -
PDVLPVKRDSVTLSIDLPG TACY_LISIV 141 -1 -
PDVLPVKRDSLTLSIDLPG TACY_LISMO 142 -1 -
PSLVMAARKPLDISIDLPG TACY_PAEAL 121 -1 -
PTLISIARGDLTLSLNLPG O85102 114 -1 -
PTLISIARGDLTLSLNLPG Q55996 114 -1 -
PTLLAVDRAPMTYSIDLPG TACY_STRPN 86 -1 -
PTSIGIARAPQTVSVDLPG O31241 147 -1 -

Motif 5 width=16
Element Seqn Id St Int Rpt
ARTQYTESMVYSKSQI TACY_STREQ 251 39 -
ARTQYTESMVYSKSQI TACY_STRPY 248 39 -
ARTQYTESMVYSKSQI TACY_STRCB 251 39 -
ARTQYSESMVYSKSQI TACY_CLOPE 177 39 -
AKIDYSDEMAYSESQL TACY_LISSE 203 41 -
AKIDYDQEMAYSESQL TACY_LISIV 201 41 -
AKIDYDDEMAYSESQL TACY_LISMO 202 41 -
ARLQYAESMVYSQNQI TACY_PAEAL 179 39 -
AELQYDETMAYSMSQL O85102 174 41 -
AELQYDETMAYSMSQL Q55996 174 41 -
ARMQYEKITAHSMEQL TACY_STRPN 145 40 -
AKISYDETMVTSKRQL O31241 206 40 -

Motif 6 width=24
Element Seqn Id St Int Rpt
GVSNEAPPLFVSNVAYGRTVFVKL TACY_STREQ 332 65 -
GVSNEAPPLFVSNVAYGRTVFVKL TACY_STRPY 329 65 -
GVSNEAPPLFVSNVAYGRTVFVKL TACY_STRCB 332 65 -
GVSNEAPPLMVSNVAYGRTIYVKL TACY_CLOPE 258 65 -
GVNAENPPAYISSVAYGRQVYVKL TACY_LISSE 284 65 -
GVNAENPPAYISSVAYGRDIFVKL TACY_LISIV 282 65 -
GVNAENPPAYISSVAYGRQVYLKL TACY_LISMO 283 65 -
GVSNEAPPLMVSNVAYGRTIYVKL TACY_PAEAL 260 65 -
GITDEVPPVYVSSVSYGRSMFIKL O85102 255 65 -
GITDEVPPVYVSSVSYGRSMFIKL Q55996 255 65 -
GISAERPLVYISSVAYGRQVYLKL TACY_STRPN 226 65 -
GVNNKNPLGYISSVSYGRQIFVKL O31241 287 65 -

Motif 7 width=18
Element Seqn Id St Int Rpt
DILENSSFTAVVLGGDAA TACY_STREQ 385 29 -
DILENSSFTAVVLGGDAA TACY_STRPY 382 29 -
DILENSSFTAVVLGADAA TACY_STRCB 385 29 -
DIYENSSFTAVVLGGDAQ TACY_CLOPE 311 29 -
NIIKNSSFKAVIYGGSAK TACY_LISSE 337 29 -
NIIQNASFKAVIYGGSAK TACY_LISIV 335 29 -
NIIKNSSFKAVIYGGSAK TACY_LISMO 336 29 -
DIYENSSFTAVVLGGDAQ TACY_PAEAL 313 29 -
DILKNTSFSAYIFGGDAG O85102 308 29 -
DILKNTSFSAYIFGGDAG Q55996 308 29 -
QILDNTEVKAVILGGDPS TACY_STRPN 279 29 -
DILNKTRATVYAVGGSAR O31241 344 33 -

Motif 8 width=28
Element Seqn Id St Int Rpt
PISYTSVFLKNNKIAGVNNRSEYVETTS TACY_STREQ 433 30 -
PISYTSVFLKNNKIAGVNNRTEYVETTS TACY_STRPY 430 30 -
PISYTSVFLKNNKIAGVNNRSEYVETTS TACY_STRCB 433 30 -
PISYTSVFLKDNSVAAVHNKTDYIETTS TACY_CLOPE 359 30 -
PISYTTNFLKDNDLAVVKNNSEYIETTS TACY_LISSE 385 30 -
PIAYTTNFLKDNQLAVVKNNSEYIETTS TACY_LISIV 383 30 -
PIAYTTNFLKDNELAVIKNNSEYIETTS TACY_LISMO 384 30 -
PISYTSVFLKDNSIAAVHNNTEYIETKT TACY_PAEAL 361 30 -
PISYSTNFVKDNRPAQILSNSEYIETTS O85102 356 30 -
PISYSTNFVKDNRPAQILSNSEYIETTS Q55996 356 30 -
PISYTTSFLRDNVVATFQNSTDYVETKV TACY_STRPN 327 30 -
PVSYAVNFLKDNQLAAVRSSGDYIETTA O31241 392 30 -

Motif 9 width=18
Element Seqn Id St Int Rpt
HQGAYVAQYEILWDEINY TACY_STREQ 472 11 -
HQGAYVAQYEILWDEINY TACY_STRPY 469 11 -
HQGAYVAQYEILWDEINY TACY_STRCB 472 11 -
HSGAYVAQFEVAWDEVSY TACY_CLOPE 398 11 -
HSGGYVAQFNISWDEVSY TACY_LISSE 424 11 -
HSGAYVARFNVTWDEVSY TACY_LISIV 422 11 -
HSGGYVAQFNISWDEVNY TACY_LISMO 423 11 -
HSGAYVAQFEVYWDEFSY TACY_PAEAL 400 11 -
HSGAYVAKYNITWEEVSY O85102 395 11 -
HSGAYVAKYNITWEEVSY Q55996 395 11 -
HSGAYVAQYYITWDELSY TACY_STRPN 366 11 -
HGGGYVAKFRLKWDEISY O31241 431 11 -

Motif 10 width=17
Element Seqn Id St Int Rpt
YDDKGKEVITKRRWDNN TACY_STREQ 489 -1 -
YDDKGKEVITKRRWDNN TACY_STRPY 486 -1 -
YDDKGKEVITKRRWDNN TACY_STRCB 489 -1 -
YDKEGNEVLTHKTWDGN TACY_CLOPE 415 -1 -
YDENGNEIKVHKKWGEN TACY_LISSE 441 -1 -
YDANGNEVVEHKKWSEN TACY_LISIV 439 -1 -
YDPEGNEIVQHKNWSEN TACY_LISMO 440 -1 -
YDADGQEIVTRKSWDGN TACY_PAEAL 417 -1 -
YNEAGEEVWEPKAWDKN O85102 412 -1 -
YNEAGEEVWEPKAWDKN Q55996 412 -1 -
YDHQGKEVLTPKAWDRN TACY_STRPN 383 -1 -
YDPQGKEIRTPKTWSGN O31241 448 -1 -

Motif 11 width=21
Element Seqn Id St Int Rpt
ERDVKLSKEINVNISGSTLSP TACY_STREQ 547 41 -
ERDVKLSKEINVNISGSTLSP TACY_STRPY 544 41 -
ERDVKLSKEINVNISGSTLSP TACY_STRCB 547 41 -
EYDVPLTNNINVSIWGTTLYP TACY_CLOPE 473 41 -
DRNLPLVKNRNVSIWGTTLYP TACY_LISSE 499 41 -
DRNLPLVKNRNVCIWGTTLYP TACY_LISIV 497 41 -
DRNLPLVKNRNISIWGTTLYP TACY_LISMO 498 41 -
EYNVPLASDINVSIWGTTLYP TACY_PAEAL 475 41 -
DKDLPLVGQRKITIWGTTLYP O85102 469 40 -
DKDLPLVGQRKITIWGTTLYP Q55996 469 40 -
KTDLPLVRKRTISIWGTTLYP TACY_STRPN 441 41 -
KKNLPLVPHREIVLKGTTLNP O31241 506 41 -