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PR01398

Identifier
ISCHRISMTASE  [View Relations]  [View Alignment]  
Accession
PR01398
No. of Motifs
6
Creation Date
15-SEP-2000
Title
Isochorismatase signature
Database References

PFAM; PF00857 Isochorismatase
INTERPRO; IPR000868
Literature References
1. PAYNE, S.M.
Detection, isolation, and characterisation of siderophores.
METHODS ENZYMOL. 235 329-344 (1994).
 
2. NAHLIK, M.S., BRICKMAN, T.J., OZENBERGER, B.A. AND MCINTOSH, M.A.
Nucleotide sequence and transcriptional organisation of the Escherichia 
coli enterobactin biosynthesis cistrons entB and entA.
J.BACTERIOL. 171 784-790 (1989).
 
3. WYCKOFF, E.E., STOEBNER, J.A., REED, K.E. AND PAYNE, S.M. 
Cloning of a Vibrio cholera vibriobactin gene cluster: identification of
genes required for early steps in siderophore synthesis.
J.BACTERIOL. 179 7055-7062 (1997).
 
4. LIU, J., DUNCAN, K. AND WALSH, C.T.
Nucleotide sequence of a cluster of Escherichia coli enterobactin 
biosynthesis genes: identification of entA and purification of its product
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase.
J.BACTERIOL. 171 791-798 (1989).

Documentation
Iron is essential for growth in both bacteria and mammals. Controlling the
amount of free iron in solution is often used as a tactic by hosts to limit
invasion of pathogenic microbes; binding iron tightly within protein
molecules can accomplish this. Such iron-protein complexes include haem in
blood, lactoferrin in tears/saliva and transferrin in blood plasma. Some
bacteria express surface receptors to capture eukaryotic iron-binding
compounds, while others have evolved siderophores to scavenge iron from
iron-binding host proteins [1].
 
The absence of free iron molecules in the surrounding environment triggers 
transcription of gene clusters that encode both siderophore-synthesis 
enzymes, and receptors that recognise iron-bound siderophores [2]. Classic
examples are the enterobactin/enterochelin clusters found in Escherichia
coli and Salmonella spp., although similar moieties in other pathogens have 
been identified. The enzymic machinery that produces vibrionectin in Vibrio
cholera is such a homologue [3].
 
EntB, an isochorismate enzyme, is involved in the second stage of 
enterobactin biosynthesis. It has a molecular weight of 35kDa, and is 
believed to possess bifunctional activity. Deletion studies involving EntB- 
mutants have shown that it is essential for virulence [4].
 
ISCHRISMTASE is a 6-element fingerprint that provides a signature for  
isochorismatases. The fingerprint was derived from an initial alignment
of 4 sequences: the motifs were drawn from conserved regions spanning the
full alignment length (~190 amino acids). Two iterations on SPTR37_10f were
required to reach convergence, at which point a true set comprising 7
sequences was identified. 
Summary Information
7 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6777777
5000000
4000000
3000000
2000000
123456
True Positives
DHBB_BACSU    ENTB_ECOLI    O33409        PHZA_PSEAR    
PHZD_PSEFL VENB_VIBVU VIBB_VIBCH
Sequence Titles
DHBB_BACSU  ISOCHORISMATASE (EC 3.3.2.1) (2,3 DIHYDRO-2,3 DIHYDROXYBENZOATE SYNTHASE) (SUPEROXIDE-INDUCIBLE PROTEIN 1) (SOI1) - BACILLUS SUBTILIS. 
ENTB_ECOLI ISOCHORISMATASE (EC 3.3.2.1) (2,3 DIHYDRO-2,3 DIHYDROXYBENZOATE SYNTHASE) - ESCHERICHIA COLI.
O33409 PCND - PSEUDOMONAS AERUGINOSA.
PHZA_PSEAR PROBABLE ISOCHORISMATASE (EC 3.3.2.1) (2,3 DIHYDRO-2,3 DIHYDROXYBENZOATE SYNTHASE) - PSEUDOMONAS AUREOFACIENS.
PHZD_PSEFL PROBABLE ISOCHORISMATASE (EC 3.3.2.1) (2,3 DIHYDRO-2,3 DIHYDROXYBENZOATE SYNTHASE) - PSEUDOMONAS FLUORESCENS.
VENB_VIBVU PROBABLE ISOCHORISMATASE (EC 3.3.2.1) (2,3 DIHYDRO-2,3 DIHYDROXYBENZOATE SYNTHASE) - VIBRIO VULNIFICUS.
VIBB_VIBCH VIBRIOBACTIN-SPECIFIC ISOCHORISMATASE (EC 3.3.2.1) (2,3 DIHYDRO-2,3 DIHYDROXYBENZOATE SYNTHASE) - VIBRIO CHOLERAE.
Scan History
SPTR37_10f 1  50   NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
AIPAIQPYQMPTA DHBB_BACSU 2 2 -
AIPKLQAYALPES ENTB_ECOLI 2 2 -
AIPKIASYPLPVS VIBB_VIBCH 2 2 -
GIPSIVPYALPTS PHZD_PSEFL 3 3 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LKNQCVQLGIPVVYTAQP DHBB_BACSU 63 48 -
LRDYCKQHNIPVYYTAQP ENTB_ECOLI 63 48 -
LKAHAKQAGIPVVYTAQP VIBB_VIBCH 61 46 -
IRQWAADNGVPVAYTAQP PHZD_PSEFL 62 46 -

Motif 3 width=20
Element Seqn Id St Int Rpt
PGSQNPDDRALLTDFWGPGL DHBB_BACSU 80 -1 -
PKEQSDEDRALLNDMWGPGL ENTB_ECOLI 80 -1 -
PANQDPAERALLSDFWGPGL VIBB_VIBCH 78 -1 -
PGSMNEEQRGLLKDFWGPGM PHZD_PSEFL 79 -1 -

Motif 4 width=23
Element Seqn Id St Int Rpt
LAPEDDDLVLTKWRYSAFKRTNL DHBB_BACSU 112 12 -
LTPDADDTVLVKWRYSAFHRSPL ENTB_ECOLI 112 12 -
LAPESGDVQLTKWRYSAFKKSPL VIBB_VIBCH 107 9 -
LAPQPGDWLLTKWRYSAFFNSDL PHZD_PSEFL 111 12 -

Motif 5 width=19
Element Seqn Id St Int Rpt
DIKAFFVGDAVADFSLEKH DHBB_BACSU 168 33 -
DIKPFMVADALADFSRDEH ENTB_ECOLI 168 33 -
DIQPFVIGDGVADFSLSDH VIBB_VIBCH 163 33 -
DIQPFLVADAIADFSKEHH PHZD_PSEFL 167 33 -

Motif 6 width=18
Element Seqn Id St Int Rpt
HQMALEYAAGRCAFTVMT DHBB_BACSU 186 -1 -
HLMSLKYVAGRSGRVVMT ENTB_ECOLI 186 -1 -
HEFSLRYISGRTGAVKST VIBB_VIBCH 181 -1 -
HWMAMEYAASRCAMVITT PHZD_PSEFL 185 -1 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
AIPAIQPYQMPTA DHBB_BACSU 2 2 -
AIPKLQAYALPES ENTB_ECOLI 2 2 -
AIPKIASYPLPVS VIBB_VIBCH 2 2 -
GIPSIVPYALPTS PHZD_PSEFL 3 3 -
GIPSIVPYALPTN PHZA_PSEAR 3 3 -
GIPEITAYPLPTA O33409 3 3 -
GIPKIAGYPLPTP VENB_VIBVU 2 2 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LKNQCVQLGIPVVYTAQP DHBB_BACSU 63 48 -
LRDYCKQHNIPVYYTAQP ENTB_ECOLI 63 48 -
LKAHAKQAGIPVVYTAQP VIBB_VIBCH 61 46 -
IRQWAADNGVPVAYTAQP PHZD_PSEFL 62 46 -
IRQWAADNGVPVAYTAQP PHZA_PSEAR 62 46 -
LRRWCVEQGVQIAYTAQP O33409 62 46 -
LKAAAKKAGIPVIYTAQP VENB_VIBVU 63 48 -

Motif 3 width=20
Element Seqn Id St Int Rpt
PGSQNPDDRALLTDFWGPGL DHBB_BACSU 80 -1 -
PKEQSDEDRALLNDMWGPGL ENTB_ECOLI 80 -1 -
PANQDPAERALLSDFWGPGL VIBB_VIBCH 78 -1 -
PGSMNEEQRGLLKDFWGPGM PHZD_PSEFL 79 -1 -
PGSMSEEQRGLLKDFWGPGM PHZA_PSEAR 79 -1 -
PGSMTEEQRGLLKDFWGPGM O33409 79 -1 -
PANQHPTDRALLTDFWGPGL VENB_VIBVU 80 -1 -

Motif 4 width=23
Element Seqn Id St Int Rpt
LAPEDDDLVLTKWRYSAFKRTNL DHBB_BACSU 112 12 -
LTPDADDTVLVKWRYSAFHRSPL ENTB_ECOLI 112 12 -
LAPESGDVQLTKWRYSAFKKSPL VIBB_VIBCH 107 9 -
LAPQPGDWLLTKWRYSAFFNSDL PHZD_PSEFL 111 12 -
LTPKPGDWLLTKWRYSAFFNSDL PHZA_PSEAR 111 12 -
LAPGPDDWLLTKWRYSAFFHSDL O33409 111 12 -
LSPEEDDIEYVKWRYSAFKKTPL VENB_VIBVU 110 10 -

Motif 5 width=19
Element Seqn Id St Int Rpt
DIKAFFVGDAVADFSLEKH DHBB_BACSU 168 33 -
DIKPFMVADALADFSRDEH ENTB_ECOLI 168 33 -
DIQPFVIGDGVADFSLSDH VIBB_VIBCH 163 33 -
DIQPFLVADAIADFSKEHH PHZD_PSEFL 167 33 -
DIQPFLVADAIADFSKEHH PHZA_PSEAR 167 33 -
DIQPFLVADAIADFSEAHH O33409 167 33 -
DIQPFVIGDAIADFTREDH VENB_VIBVU 166 33 -

Motif 6 width=18
Element Seqn Id St Int Rpt
HQMALEYAAGRCAFTVMT DHBB_BACSU 186 -1 -
HLMSLKYVAGRSGRVVMT ENTB_ECOLI 186 -1 -
HEFSLRYISGRTGAVKST VIBB_VIBCH 181 -1 -
HWMAMEYAASRCAMVITT PHZD_PSEFL 185 -1 -
HWMAIEYAASRCAMVITT PHZA_PSEAR 185 -1 -
HRMALEYAASRCAMVVTT O33409 185 -1 -
HLRTLQYVASRSGSVKRL VENB_VIBVU 184 -1 -