SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01394

Identifier
CLENTEROTOXN  [View Relations]  [View Alignment]  
Accession
PR01394
No. of Motifs
7
Creation Date
31-MAY-2000
Title
Clostridium enterotoxin signature
Database References
Literature References
1. CZECZULIN, J.R., HANNA, P.C. AND MCCLANE, B.A.
Cloning, nucleotide sequencing, and expression of the Clostridium 
perfringens enterotoxin gene in Escherichia coli
INFECT.IMMUN. 61 3429-3439 (1993).
 
2. KATAHIRA, J., INOUE, N., HORIGUCHI, Y., MATSUDA, M. AND SUGIMOTO N.
Molecular cloning and functional characterisation of the receptor for the 
Clostridium perfringens enterotoxin
J.CELL BIOL. 136 1239-1247 (1997).
 
3. KATAHIRA, J., SUGIYAMA, H., INOUE, N., HORIGUCHI, Y., MATSUDA, M. AND
SUGIMOTO, N.
Clostridium perfringens enterotoxin utilizes two structurally related 
membrane proteins as functional receptors in vivo.
J.BIOL.CHEM. 272 26652-26658 (1997).

Documentation
Clostridial species are one of the major causes of food poisoning/gastro-
intestinal illnesses. They are Gram-positive, spore-forming rods that occur
naturally in the soil [1]. Among the family are: C.botulinum, which produces
one of the most potent toxins in existence; C.tetani, causative agent of
tetanus; and C.perfringens, commonly found in wound infections and diarrhoea
cases. The use of toxins to damage the host is a method deployed by many
bacterial pathogens.
 
The major virulence factor of Clostridium perfringens is the CPE enterotoxin,
which is secreted upon invasion of the host gut, and contributes to food 
poisoning and other gastrointestinal illnesses [1]. It has a molecular 
weight of 35.3kDa, and is responsible for the disintegration of tight 
junctions between endothelial cells in the gut [2]. This mechanism is 
mediated by host claudins-3 and -4, situated at the tight junctions [2]. 
 
Recently, two more host receptors have been characterised and expressed in 
vivo [3]. Named CPE-R and RVP1, these may be utilised in the passage of 
Clostridial species through the gut wall, although the regulatory mechanisms
have not been elucidated.
 
CLENTEROTOXN is a 7-element fingerprint that provides a signature for 
Clostridium enterotoxins. The fingerprint was derived from an initial 
alignment of 3 sequences: the motifs were drawn from conserved regions
spanning the full alignment length (~610 amino acids). Two iterations on
SPTR37_10f  were required to reach convergence, at which point a true set  
comprising 8 sequences was identified. 
Summary Information
8 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
78888888
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
ELTB_CLOPE    HA70_CLOBO    O32373        O52925        
Q05130 Q45877 Q46182 Q9ZX79
Sequence Titles
ELTB_CLOPE  HEAT-LABILE ENTEROTOXIN B CHAIN PRECURSOR - CLOSTRIDIUM PERFRINGENS. 
HA70_CLOBO HEMAGGLUTININ COMPONENTS HA-53 AND HA-22/23 PRECURSOR - CLOSTRIDIUM BOTULINUM.
O32373 ENTEROTOXIN - CLOSTRIDIUM PERFRINGENS.
O52925 ENTEROTOXIN - CLOSTRIDIUM PERFRINGENS.
Q05130 ENTEROTOXIN (CPE) - CLOSTRIDIUM PERFRINGENS.
Q45877 HA70 - CLOSTRIDIUM BOTULINUM.
Q46182 ENTEROTOXIN - CLOSTRIDIUM PERFRINGENS.
Q9ZX79 HA3 - CLOSTRIDIUM BOTULINUM D PHAGE.
Scan History
SPTR37_10f 2  15   NSINGLE    
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
EPSVVSSQILNPNETGTFSQ O52925 54 54 -
EPSVLSSQILNPNETGTFSQ ELTB_CLOPE 57 57 -
DKDLIGTLLIESSTSGSIIQ HA70_CLOBO 227 227 -

Motif 2 width=20
Element Seqn Id St Int Rpt
AGPNEYVYYKVYATYRKYQA O52925 122 48 -
AGPNEYVYYKVYATYRKYQA ELTB_CLOPE 113 36 -
APTNKNISMKVYNTYEKYEL HA70_CLOBO 296 49 -

Motif 3 width=15
Element Seqn Id St Int Rpt
SHGNISDDGSIYKLT O52925 145 3 -
SHGNISDDGSIYKLT ELTB_CLOPE 136 3 -
QNGNIDDKAEYYLPS HA70_CLOBO 319 3 -

Motif 4 width=19
Element Seqn Id St Int Rpt
DQGSLIETGERCVLTVPST O52925 175 15 -
DQGSLIETGERCVLTVPST ELTB_CLOPE 166 15 -
DQDGFIQTGPNENIIVGVI HA70_CLOBO 355 21 -

Motif 5 width=15
Element Seqn Id St Int Rpt
NLTDALNSNPAGNLY O52925 210 16 -
NLTDALNSNPAGNLY ELTB_CLOPE 201 16 -
NIPTSIQNNACYVLF HA70_CLOBO 393 19 -

Motif 6 width=13
Element Seqn Id St Int Rpt
SSNSYPWTQKLNL O52925 228 3 -
SSNSYPWTQKLNL ELTB_CLOPE 219 3 -
TTGVYKITTKNNL HA70_CLOBO 411 3 -

Motif 7 width=12
Element Seqn Id St Int Rpt
YVDISLDAGQYV O52925 286 45 -
YVDLSLDAGQYV ELTB_CLOPE 276 44 -
YTRQSPDVGNYI HA70_CLOBO 523 99 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
EPSVVSSQILNPNETGTFSQ O32373 54 54 -
EPSVVSSQILNPNETGTFSQ Q05130 54 54 -
EPSVVSSQILNPNETGTFSQ O52925 54 54 -
EPSVVSSQILNPNETGTFSQ Q46182 54 54 -
EPSVLSSQILNPNETGTFSQ ELTB_CLOPE 57 57 -
DKDLIGTLLIESSTSGSIIQ HA70_CLOBO 227 227 -
DKDLIGTLLIESSTSGSIIQ Q9ZX79 227 227 -
DKDLIGTLLIEAGSSGSIIQ Q45877 230 230 -

Motif 2 width=20
Element Seqn Id St Int Rpt
AGPNEYVYYKVYATYRKYQA O32373 122 48 -
AGPNEYVYYKVYATYRKYQA Q05130 122 48 -
AGPNEYVYYKVYATYRKYQA O52925 122 48 -
AGPNEYVYYKVYATYRKYQA Q46182 122 48 -
AGPNEYVYYKVYATYRKYQA ELTB_CLOPE 113 36 -
APTNKNISMKVYNTYEKYEL HA70_CLOBO 296 49 -
APTNKNISMKVYNTYEKYEL Q9ZX79 296 49 -
APSDKNICIKAYNTYEKYEL Q45877 299 49 -

Motif 3 width=15
Element Seqn Id St Int Rpt
SHGNISDDGSIYKLT O32373 145 3 -
SHGNISDDGSIYKLT Q05130 145 3 -
SHGNISDDGSIYKLT O52925 145 3 -
SHGNISDSGSIYKLT Q46182 145 3 -
SHGNISDDGSIYKLT ELTB_CLOPE 136 3 -
QNGNIDDKAEYYLPS HA70_CLOBO 319 3 -
QNGNIDDKAEYYLPS Q9ZX79 319 3 -
QNGSIVNKAEYYLPS Q45877 322 3 -

Motif 4 width=19
Element Seqn Id St Int Rpt
DQGSLIETGERCVLTVPST O32373 175 15 -
DQGSLIETGERCVLTVPST Q05130 175 15 -
DQGSLIETGERCVLTVPST O52925 175 15 -
DQGSLIETGERCVLTVPST Q46182 175 15 -
DQGSLIETGERCVLTVPST ELTB_CLOPE 166 15 -
DQDGFIQTGPNENIIVGVI HA70_CLOBO 355 21 -
DQDGFIQTGPNENIIVGVI Q9ZX79 355 21 -
AEDGFIQNGPEEEIVVGVI Q45877 358 21 -

Motif 5 width=15
Element Seqn Id St Int Rpt
NLTDALNSNPAGNLY O32373 210 16 -
NLTDALNSNPAGNLY Q05130 210 16 -
NLTDALNSNPAGNLY O52925 210 16 -
NLTDALNSNPAGNLY Q46182 210 16 -
NLTDALNSNPAGNLY ELTB_CLOPE 201 16 -
NIPTSIQNNACYVLF HA70_CLOBO 393 19 -
NIPTSIQNNACYVLF Q9ZX79 393 19 -
NIPGIVNNNPFYILF Q45877 396 19 -

Motif 6 width=13
Element Seqn Id St Int Rpt
SSNSYPWTQKLNL O32373 228 3 -
SSNSYPWTQKLNL Q05130 228 3 -
SSNSYPWTQKLNL O52925 228 3 -
SSNSYPWTQKLNL Q46182 228 3 -
SSNSYPWTQKLNL ELTB_CLOPE 219 3 -
TTGVYKITTKNNL HA70_CLOBO 411 3 -
TTGVYKITTKNNL Q9ZX79 411 3 -
TTGIYKINTQNNL Q45877 414 3 -

Motif 7 width=12
Element Seqn Id St Int Rpt
YVDISLDAGQYV O32373 286 45 -
YVDISLDAGQYV Q05130 286 45 -
YVDISLDAGQYV O52925 286 45 -
YVDISLDAGQYV Q46182 286 45 -
YVDLSLDAGQYV ELTB_CLOPE 276 44 -
YTRQSPDVGNYI HA70_CLOBO 523 99 -
YTRQSPDVGNYI Q9ZX79 523 99 -
YTRQSPDVGNYI Q45877 526 99 -