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PR01392

Identifier
ANTHRAXTOXNA  [View Relations]  [View Alignment]  
Accession
PR01392
No. of Motifs
10
Creation Date
07-JUN-2000
Title
Anthrax toxin LF subunit signature 
Database References
Literature References
1. PERELLE, S., GILBERT, M., BOQUET, P. AND POPOFF, M.R.
Characterisation of Clostridium perfringens iota-toxin genes and 
expression in Escherichia coli. 
INFECT.IMMUN. 61 5147-5156 (1993).
 
2. ROBERTSON, D.L., TIPPETS, M.T.T. AND LEPPLA, S.H.
Nucleotide sequence of the Bacillus anthracis edema factor gene (cya): a
calmodulin-dependent adenylate cyclase.
GENE 73 363-371 (1988).
 
3. BRAGG, T.S. AND ROBERTSON, D.L.
Nucleotide sequence and analysis of the lethal factor gene (lef) from 
Bacillus anthracis.
GENE 81 45-54 (1989).
 
4. PEARD, C., BERCHE, P. AND MOCK, M.
Contribution of individual toxin components to virulence of Bacillus 
anthracis
INFECT.IMMUN. 59 3472-3477 (1991).
 
5. KOCHI, S.K., SCHIAVO, G., MOCK, M. AND MONTECUCCO, C.
Zinc content of the Bacillus anthracis lethal factor.
FEMS.MIRCOBIOL.LETT. 124 343-348 (1994).

Documentation
A large group of bacterial exotoxins are referred to as "A/B toxins", 
essentially because they are formed from two subunits [1]. The "A" subunit
possesses enzyme activity, and is transferred to the host cell following
a conformational change in the membrane-bound transport "B" subunit [1].
 
Bacillus anthracis, a large Gram-positive spore-forming rod, is the 
causative agent of anthrax [2]. Its two virulence factors are the 
poly-D-glutamate polypeptide capsule, and the actual anthrax exotoxin [4].
The toxin comprises three factors: the protective antigen (PA); the oedema
factor (EF) [2]; and the lethal factor (LF) [3]. Each is a thermolabile 
protein of ~80kDa. PA forms the "B" part of the exotoxin and allows passage
of the "A" moiety (consisting of EF and LF) into target cells.
 
EF is necessary for the oedema-producing activity of the toxin, and is 
known to be an inherent adenylate cyclase. It causes dysregulation of 
intracellular signalling [2,4]. Uptake of the lethal factor LF occurs via 
activated heptameric PA. Once inside the host lymphocytes/macrophages, the
zinc metalloprotease of LF [5] cleaves MAP kinases, inhibits cell 
proliferation, and leads to cell death [3,4]. 
 
ANTHRAXTOXNA is a 10-element fingerprint that provides a signature for 
Bacillus anthracis anthrax toxin LF proteins. The fingerprint was derived
from an initial alignment of 2 sequences: the motifs were drawn from 
conserved regions spanning the N-terminal half of the alignment. A single
iteration on SPTR37_10f was required to reach convergence, no further
sequences being identified beyond the starting set.
Summary Information
2 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
102222222222
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
CYAA_BACAN    LEF_BACAN     
Sequence Titles
CYAA_BACAN  CALMODULIN-SENSITIVE ADENYLATE CYCLASE PRECURSOR (EC 4.6.1.1) (ATP PYROPHOSPHATE-LYASE) (ADENYLYL CYCLASE) (EDEMA FACTOR) (EF) - BACILLUS ANTHRACIS. 
LEF_BACAN LETHAL FACTOR PRECURSOR (EC 3.4.24.-) (LF) - BACILLUS ANTHRACIS.
Scan History
SPTR37_10f 1  15   NSINGLE    
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
IISFSVLLFAISSS CYAA_BACAN 13 13 -
VISMSCLVTAITLS LEF_BACAN 10 10 -

Motif 2 width=21
Element Seqn Id St Int Rpt
EKNKTEKEKFKDSINNLVKTE CYAA_BACAN 50 23 -
ERNKTQEEHLKEIMKHIVKIE LEF_BACAN 60 36 -

Motif 3 width=24
Element Seqn Id St Int Rpt
LLKKIPKDVLEIYSELGGEIYFTD CYAA_BACAN 85 14 -
LLEKVPSDVLEMYKAIGGKIYIVD LEF_BACAN 95 14 -

Motif 4 width=19
Element Seqn Id St Int Rpt
HKELQDLSEEEKNSMNSRG CYAA_BACAN 114 5 -
HISLEALSEDKKKIKDIYG LEF_BACAN 124 5 -

Motif 5 width=24
Element Seqn Id St Int Rpt
GEKVPFASRFVFEKKRETPKLIIN CYAA_BACAN 132 -1 -
GKDALLHEHYVYAKEGYEPVLVIQ LEF_BACAN 142 -1 -

Motif 6 width=27
Element Seqn Id St Int Rpt
DYAINSEQSKEVYYEIGKGISLDIISK CYAA_BACAN 158 2 -
DYVENTEKALNVYYEIGKILSRDILSK LEF_BACAN 169 3 -

Motif 7 width=17
Element Seqn Id St Int Rpt
DDSDSSDLLFSQKFKEK CYAA_BACAN 201 16 -
SDSDGQDLLFTNQLKEH LEF_BACAN 214 18 -

Motif 8 width=19
Element Seqn Id St Int Rpt
NLTEFQHAFSLAFSYYFAP CYAA_BACAN 233 15 -
NSNEVQEVFAKAFAYYIEP LEF_BACAN 242 11 -

Motif 9 width=20
Element Seqn Id St Int Rpt
PDHRTVLELYAPDMFEYMNK CYAA_BACAN 251 -1 -
PQHRDVLQLYAPEAFNYMDK LEF_BACAN 260 -1 -

Motif 10 width=21
Element Seqn Id St Int Rpt
NLLIKYGIERKPDSTKGTLSN CYAA_BACAN 531 260 -
NERLKWRIQLSPDTRAGYLEN LEF_BACAN 529 249 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
IISFSVLLFAISSS CYAA_BACAN 13 13 -
VISMSCLVTAITLS LEF_BACAN 10 10 -

Motif 2 width=21
Element Seqn Id St Int Rpt
EKNKTEKEKFKDSINNLVKTE CYAA_BACAN 50 23 -
ERNKTQEEHLKEIMKHIVKIE LEF_BACAN 60 36 -

Motif 3 width=24
Element Seqn Id St Int Rpt
LLKKIPKDVLEIYSELGGEIYFTD CYAA_BACAN 85 14 -
LLEKVPSDVLEMYKAIGGKIYIVD LEF_BACAN 95 14 -

Motif 4 width=19
Element Seqn Id St Int Rpt
HKELQDLSEEEKNSMNSRG CYAA_BACAN 114 5 -
HISLEALSEDKKKIKDIYG LEF_BACAN 124 5 -

Motif 5 width=24
Element Seqn Id St Int Rpt
GEKVPFASRFVFEKKRETPKLIIN CYAA_BACAN 132 -1 -
GKDALLHEHYVYAKEGYEPVLVIQ LEF_BACAN 142 -1 -

Motif 6 width=27
Element Seqn Id St Int Rpt
DYAINSEQSKEVYYEIGKGISLDIISK CYAA_BACAN 158 2 -
DYVENTEKALNVYYEIGKILSRDILSK LEF_BACAN 169 3 -

Motif 7 width=17
Element Seqn Id St Int Rpt
DDSDSSDLLFSQKFKEK CYAA_BACAN 201 16 -
SDSDGQDLLFTNQLKEH LEF_BACAN 214 18 -

Motif 8 width=19
Element Seqn Id St Int Rpt
NLTEFQHAFSLAFSYYFAP CYAA_BACAN 233 15 -
NSNEVQEVFAKAFAYYIEP LEF_BACAN 242 11 -

Motif 9 width=20
Element Seqn Id St Int Rpt
PDHRTVLELYAPDMFEYMNK CYAA_BACAN 251 -1 -
PQHRDVLQLYAPEAFNYMDK LEF_BACAN 260 -1 -

Motif 10 width=21
Element Seqn Id St Int Rpt
NLLIKYGIERKPDSTKGTLSN CYAA_BACAN 531 260 -
NERLKWRIQLSPDTRAGYLEN LEF_BACAN 529 249 -