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PR01387

Identifier
CDTOXINA  [View Relations]  [View Alignment]  
Accession
PR01387
No. of Motifs
6
Creation Date
07-JUN-2000
Title
Cytolethal distending toxin A signature
Database References
Literature References
1. PICKETT, C.L., COTTLE, D.L., PESCI, E.C. AND BIKAH, G.
Cloning, sequencing, and expression of the Escherichia coli cytolethal
distending toxin genes.
INFECT.IMMUN. 62 1046-1051 (1994).
 
2. EYIGOR, I., DAWSON, K.A., LANGLOIS, B.E. AND PICKETT, C.L.
Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter 
coli isolates; detection and analysis by PCR.
J.CLIN.MICROBIOL. 37 1646-1650 (1999).
 
3. DE RYCKE, J., SERT, V., COMAYRAS, C. AND TASCA, C.
Sequence of lethal events in HeLa cells exposed to the G2 blocking 
cytolethal distending toxin.
EUR.J.CELL BIOL. 29 192-201 (2000).

Documentation
Escherichia coli, Haemophilus spp. and Campylobacter spp. all produce 
a toxin that is seen to cause distension in certain cell lines [1,2], 
which eventually disintegrate and die. This novel toxin, termed cytolethal 
distending toxin (cdt), has three subunits: A, B and C. Their sizes are 
approx. 27.7, 29.5 and 19.9kDa respectively [1], and they appear to be 
entirely novel [2]. 
 
Further research on the complete toxin has revealed that it blocks the cell
cycle at stage G2, through inactivation of the cyclin-dependent kinase Cdk1
[3], and without induction of DNA breaks. This leads to multipolar abortive 
mitosis and micronucleation, associated with centrosomal amplification [3].
The roles of each subunit are unclear, but it is believed that they have
separate roles in pathogenicity.
 
CDTOXINA is a 6-element fingerprint that provides a signature for cytolethal
distending toxin A proteins. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved regions 
spanning the full alignment length (~210 amino acids). Two iterations on
SPTR37_10f were required to reach convergence, at which point a true set
comprising 5 sequences was identified. A single partial match was also
found, Q46100, a CdtA protein from Campylobacter jejuni that matches motifs
1 and 5. 
Summary Information
   5 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
6555555
5000000
4000000
3000000
2010010
123456
True Positives
O06522        O32585        O87120        Q46668        
Q47088
True Positive Partials
Codes involving 2 elements
Q46100
Sequence Titles
O06522      CYTOLETHAL DISTENDING TOXIN PROTEIN A - HAEMOPHILUS DUCREYI. 
O32585 CYTOLETHAL DISTENDING TOXIN-IIIA - ESCHERICHIA COLI.
O87120 CYTOLETHAL DISTENDING TOXIN A - ACTINOBACILLUS SP., AND ACTINOBACILLUS ACTINOMYCETEMCOMITANS (HAEMOPHILUS ACTINOMYCETEMCOMITANS).
Q46668 CDTA - ESCHERICHIA COLI.
Q47088 CDTA - ESCHERICHIA COLI.

Q46100 CDTA - CAMPYLOBACTER JEJUNI.
Scan History
SPTR37_10f 2  25   NSINGLE    
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
PLPGPGPALPTNGA Q46668 55 55 -
PLLSKASSMPLNLL O87120 47 47 -
GIPGQGIAVTSNGA Q47088 36 36 -

Motif 2 width=18
Element Seqn Id St Int Rpt
GSVLTMWSRGAGSSLWAY Q46668 87 18 -
GALLTVWALAKRNWLWAY O87120 84 23 -
GALLTIWARPVRNWLWGY Q47088 70 20 -

Motif 3 width=15
Element Seqn Id St Int Rpt
SNSFGELRNWQIMPG Q46668 109 4 -
SQDFGNIRNWKIEPG O87120 106 4 -
SVNFGENRNWKVVDG Q47088 92 4 -

Motif 4 width=13
Element Seqn Id St Int Rpt
FRNVDVGTCMTSF Q46668 131 7 -
FVNQSLGTCIEAY O87120 128 7 -
FVNVAQGTCMEAF Q47088 114 7 -

Motif 5 width=15
Element Seqn Id St Int Rpt
NGNYQLKSLSTGLCI Q46668 173 29 -
SGAVVIKSVSQGRCV O87120 165 24 -
NGNVLIRSSALQTCI Q47088 151 24 -

Motif 6 width=15
Element Seqn Id St Int Rpt
YATTLTMERCPSSGE Q46668 200 12 -
YYSTVTLSTCDGATE O87120 188 8 -
FAFTITLEKCPGAKE Q47088 178 12 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
PLPGPGPALPTNGA Q46668 55 55 -
PLPGAGPALPTNAP O32585 55 55 -
PLLSKASSMPLNLL O87120 47 47 -
PLLSKTPSMSLNLL O06522 47 47 -
GIPGQGIAVTSNGA Q47088 36 36 -

Motif 2 width=18
Element Seqn Id St Int Rpt
GSVLTMWSRGAGSSLWAY Q46668 87 18 -
GSVLTMWSRGAGSSLWAY O32585 87 18 -
GALLTVWALAKRNWLWAY O87120 84 23 -
GALLTVWALAKRNWLWAY O06522 85 24 -
GALLTIWARPVRNWLWGY Q47088 70 20 -

Motif 3 width=15
Element Seqn Id St Int Rpt
SNSFGELRNWQIMPG Q46668 109 4 -
SNSFGELRNWQIMPG O32585 109 4 -
SQDFGNIRNWKIEPG O87120 106 4 -
SQDFGNIRNWKMEPG O06522 107 4 -
SVNFGENRNWKVVDG Q47088 92 4 -

Motif 4 width=13
Element Seqn Id St Int Rpt
FRNVDVGTCMTSF Q46668 131 7 -
FRNVDVGTCMTSF O32585 131 7 -
FVNQSLGTCIEAY O87120 128 7 -
FVNQSLGTCVEAY O06522 129 7 -
FVNVAQGTCMEAF Q47088 114 7 -

Motif 5 width=15
Element Seqn Id St Int Rpt
NGNYQLKSLSTGLCI Q46668 173 29 -
NGNYQLKSLSTGLCI O32585 173 29 -
SGAVVIKSVSQGRCV O87120 165 24 -
SGAVVIKSVSQGRCV O06522 166 24 -
NGNVLIRSSALQTCI Q47088 151 24 -

Motif 6 width=15
Element Seqn Id St Int Rpt
YATTLTMERCPSSGE Q46668 200 12 -
YATTLTMERCPSSGE O32585 200 12 -
YYSTVTLSTCDGATE O87120 188 8 -
FYSTVTLSVCDGATE O06522 189 8 -
FAFTITLEKCPGAKE Q47088 178 12 -