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PR01383

Identifier
CLAUDIN10  [View Relations]  [View Alignment]  
Accession
PR01383
No. of Motifs
4
Creation Date
27-JUL-2000
Title
Claudin-10 signature
Database References
PRINTS; PR01077 CLAUDIN
Literature References
1. TSUKITA, S. AND FURUSE, M.
Occuldin and claudins in tight-junction strands: leading or supporting
players?
TRENDS CELL BIOL. 9 268-273 (1999).
 
2. FURUSE, M., FUJITA, K., HIIRAGI, T., FUJIMOTO, K. AND TSUKITA, S.
Claudin-1 and -2: novel integral membrane proteins localizing at tight 
junctions with no sequence similarity to occludin.
J.CELL BIOL. 141 1539-1550 (1998).
 
3.FURUSE, M., SASAKI, H., FUJIMOTO, K. AND TSUKITA, S.
A single gene product, claudin-1 or -2, reconstitutes tight junction 
strands and recruits occludin in fibroblasts.
J.CELL BIOL. 143 391-401 (1998).
 
4. MORITA, K., FURUSE, M., FUJIMOTO, K. AND TSUKITA, S.
Claudin multigene family encoding four-transmembrane domain protein
components of tight junction strands.
PROC.NATL.ACAD.SCI.U.S.A. 96 511-516 (1999).

Documentation
PRINTS; PR01379 CLAUDIN4; PR01380 CLAUDIN5; PR01381 CLAUDIN7
Tight junctions (TJs) are specialised membrane domains found at the most 
apical region of polarised epithelial and endothelial cells. They create a 
primary barrier, preventing paracellular transport of solutes and 
restricting lateral diffusion of membrane lipids and proteins. They also 
act as diffusion barriers within plasma membranes, creating and maintaining
apical and basolateral membrane domains.
 
Recently, the molecular architecture of tight junctions has begun to be
elucidated. One group of proteins thought to be major components of TJs 
is the claudin family [1]. Immunofluorescence studies have shown that 
claudins are targeted to and incorporated into tight junctions [2]. 
Furthermore, when claudins are introduced into cells that lack tight 
junctions, networks of strands and grooves form at cell-cell contact sites 
that closely resemble native TJs [3].
 
The claudin protein family is encoded by at least 17 human genes, with many
homologues cloned from other species. Tissue distribution patterns for the 
claudin family members are distinct. Claudin-1 and -2, for example, are 
expressed at high levels in the liver and kidney, whereas claudin-3 mRNA is
detected mainly in the lung and liver [2,4]. This suggests that multiple 
claudin family members may be involved in tight junction strand formation 
in a tissue-dependent manner.
 
Hydropathy analysis suggests that all claudins share a common transmembrane
(TM) topology. Each family member is predicted to possess four TM domains 
with intracellular N- and C-termini. Although their C-terminal cytoplasmic 
domain sequences vary, most claudin family members share a common motif of 
-Y-V in this region. This has been postulated as a possible binding motif 
for PDZ domains of other tight junction-associated membrane proteins, such 
as ZO-1.
 
Claudin-10 was identified through cDNA database searching, pursuing
sequences similar to other claudin family members [1]. Human and mouse
isoforms have been cloned. Claudin-10 shares ~20-45% overall similarity with
other claudin family members at the amino acid level, displaying highest
similarity to claudin-15.
 
CLAUDIN10 is a 4-element fingerprint that provides a signature for 
claudin-10 proteins. The fingerprint was derived from an initial alignment 
of 2 sequences: the motifs were drawn from conserved regions spanning 
virtually the full alignment length, focusing on those sections that
characterise claudin-10 and distinguish it from other family members - 
motif 1 lies in the first TM domain; motif 2 resides within in the second
TM domain; motifs 3 spans part of the fourth TM domain and part of the 
C-terminal region; and motif 4 resides within the cytoplasmic C-terminus.
A single iteration on SPTR37_10f was required to reach convergence, no
further sequences being identified beyond the starting set. Note, P78369,
annotated in TrEMBL as a putative oligodendrocyte-specific protein-like 
protein, has recently been reclassified as Homo sapiens claudin-10.
Summary Information
2 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
42222
30000
20000
1234
True Positives
P78369        Q9Z0S6        
Sequence Titles
P78369      PUTATIVE OSP LIKE PROTEIN - HOMO SAPIENS (HUMAN). 
Q9Z0S6 CLAUDIN-10 - MUS MUSCULUS (MOUSE).
Scan History
SPTR37_10f 1  150  NSINGLE    
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
VSSTLPTDY P78369 21 21 -
VSSTLPTDY Q9Z0S6 21 21 -

Motif 2 width=13
Element Seqn Id St Int Rpt
VSLGFFGSIFALF P78369 87 57 -
VSLGFFGSIFALF Q9Z0S6 87 57 -

Motif 3 width=14
Element Seqn Id St Int Rpt
GVIFCFSISDNNKT P78369 176 76 -
GVIFCFSISDNNKT Q9Z0S6 176 76 -

Motif 4 width=20
Element Seqn Id St Int Rpt
YTYNGATSVMSSRTKYHGGE P78369 192 2 -
YTYNGPTSVMSSRTKYQGGE Q9Z0S6 194 4 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
VSSTLPTDY P78369 21 21 -
VSSTLPTDY Q9Z0S6 21 21 -

Motif 2 width=13
Element Seqn Id St Int Rpt
VSLGFFGSIFALF P78369 87 57 -
VSLGFFGSIFALF Q9Z0S6 87 57 -

Motif 3 width=14
Element Seqn Id St Int Rpt
GVIFCFSISDNNKT P78369 176 76 -
GVIFCFSISDNNKT Q9Z0S6 176 76 -

Motif 4 width=20
Element Seqn Id St Int Rpt
YTYNGATSVMSSRTKYHGGE P78369 192 2 -
YTYNGPTSVMSSRTKYQGGE Q9Z0S6 194 4 -