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PR01370

Identifier
TRNSINTIMINR  [View Relations]  [View Alignment]  
Accession
PR01370
No. of Motifs
9
Creation Date
13-JUL-2000
Title
Translocated intimin receptor (Tir) signature
Database References
Literature References
1. BATCHELOR, M., PRASANNAN, S., DANIELL, S., REECE, S., CONNERTON, I.,
BLOOMBERG, G., DOUGAN, G., FRANKEL, G. AND MATTHEWS, S.
Structural basis for recognition of the translocated intimin receptor (Tir) 
by the intimin from enteropathogenic Escherichia coli.
EMBO J. 19 2452-2464 (2000).
 
2. HUECK, C.J.
Type III protein secretion systems in bacterial pathogens of animals and 
plants.
MICROBIOL.MOL.BIOL.REV. 379-433 (1998).

Documentation
Secretion of virulence factors in Gram-negative bacteria involves 
transportation of the protein across two membranes to reach the cell 
exterior [2]. There have been four secretion systems described in 
animal enteropathogens, such as Salmonella and Yersinia, with further 
sequence similarities in plant pathogens like Ralstonia and Erwinia [2].
 
The type III secretion system is of great interest, as it is used to 
transport virulence factors from the pathogen directly into the host cell 
[2] and is only triggered when the bacterium comes into close contact with
the host. The protein subunits of the system are very similar to those of 
bacterial flagellar biosynthesis [2]. However, while the latter forms a
ring structure to allow secretion of flagellin and is an integral part of
the flagellum itself [2], type III subunits in the outer membrane 
translocate secreted proteins through a channel-like structure.
 
Exotoxins secreted by the type III system do not possess a secretion signal,
and are considered unique for this reason [2]. Enteropathogenic and entero-
haemorrhagic Escherichia coli secrete the translocated intimin receptor
(Tir) [1]. The bacterial adhesion molecule, intimin, targets Tir, which is
embedded in the target cell's plasma membrane [1], and allows the 
bacterial cell to attach to the host.
 
TRNSINTIMINR is a 9-element fingerprint that provides a signature for the 
translocated intimin receptors. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions 
spanning the full alignment length (~550 amino acids). Two iterations on
SPTR37_10f were required to reach convergence, at which point a true set
comprising 6 sequences was identified.
Summary Information
6 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9666666666
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
O50190        O52147        O68258        O85506        
O85508 Q47014
Sequence Titles
O50190      TRANSLOCATED INTIMIN RECEPTOR - ESCHERICHIA COLI. 
O52147 TRANSLOCATED INTIMIN RECEPTOR - ESCHERICHIA COLI.
O68258 TRANSLOCATED INTIMIN RECEPTOR - ESCHERICHIA COLI.
O85506 TRANSLOCATED INTIMIN RECEPTOR TIR - ESCHERICHIA COLI.
O85508 TRANSLOCATED INTIMIN RECEPTOR TIR - ESCHERICHIA COLI.
Q47014 TRANSLOCATED INTIMIN RECEPTOR TIR - ESCHERICHIA COLI.
Scan History
SPTR37_10f 2  50   NSINGLE    
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
GALGSRSLFSPLRNSMADSVDSR O50190 42 42 -
GPMGSRLLFTPIRNSVADAADSR O85508 41 41 -

Motif 2 width=18
Element Seqn Id St Int Rpt
ETCLLGGFEVLHDKGPLD O50190 82 17 -
EVSLHGALEVLHDKGGLD O85508 81 17 -

Motif 3 width=17
Element Seqn Id St Int Rpt
EGKNRFVFTGGRGGSGH O50190 149 49 -
EGKNKFVFTGGRGGAGH O85508 148 49 -

Motif 4 width=19
Element Seqn Id St Int Rpt
RSDPKFWVSVGAIAAGLAG O50190 229 63 -
RADPKLWLSLGTIAAGLIG O85508 218 53 -

Motif 5 width=19
Element Seqn Id St Int Rpt
KVNIDANGNAIPSGELKDD O50190 298 50 -
KVNIDENGNAIPSGELIDD O85508 287 50 -

Motif 6 width=21
Element Seqn Id St Int Rpt
YEDQHARRQEELQLSSGIGYG O50190 347 30 -
YDEQHAKREQEMSLSSGVGYG O85508 336 30 -

Motif 7 width=21
Element Seqn Id St Int Rpt
GGIGAGVTTALHRRNQPAEQT O50190 376 8 -
GGIGAGVTAALHRKNQPAEQT O85508 365 8 -

Motif 8 width=18
Element Seqn Id St Int Rpt
PVTGRLIGTPGQGIQSTY O50190 493 96 -
PVTGRLVGTPGQGIQSTY O85508 482 96 -

Motif 9 width=15
Element Seqn Id St Int Rpt
GGLRLGMGGLTSGGE O50190 517 6 -
GGLRLGMGGLTGGGE O85508 506 6 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
GALGSRSLFSPLRNSMADSVDSR O50190 42 42 -
GALGSRSLFSPLRNSMADSVDSR O52147 42 42 -
GPMGSRLLFTPIRNSVADAADSR Q47014 41 41 -
GPMGSRLLFTPIRNSVADAADSR O85508 41 41 -
GALGSRLLFSPLRNSIADSVDSR O68258 42 42 -
GPLGSRALFTPVRNSMADSGDNR O85506 40 40 -

Motif 2 width=18
Element Seqn Id St Int Rpt
ETCLLGGFEVLHDKGPLD O50190 82 17 -
ETCLLGGFEVLHDKGPLD O52147 82 17 -
EVSLHGALEVLHDKGGLD Q47014 81 17 -
EVSLHGALEVLHDKGGLD O85508 81 17 -
EICLLGGFEVLHDKGPLD O68258 82 17 -
EITLNDGFEVLHDHGPLD O85506 80 17 -

Motif 3 width=17
Element Seqn Id St Int Rpt
EGKNRFVFTGGRGGSGH O50190 149 49 -
EGKNRFVFTGGRGGSGH O52147 149 49 -
EGKNKFVFTGGRGGAGH Q47014 148 49 -
EGKNKFVFTGGRGGAGH O85508 148 49 -
EDKGRFVFTGGRGGGGH O68258 149 49 -
EGKDKFVFTGGRGGAGH O85506 147 49 -

Motif 4 width=19
Element Seqn Id St Int Rpt
RSDPKFWVSVGAIAAGLAG O50190 229 63 -
RSDPKFWVSVGAIAAGLAG O52147 229 63 -
RADPKLWLSLGTIAAGLIG Q47014 218 53 -
RADPKLWLSLGTIAAGLIG O85508 218 53 -
RSDPKFWVSIGAIAAGLAG O68258 229 63 -
RSDPKLWLALGTVATGLIG O85506 226 62 -

Motif 5 width=19
Element Seqn Id St Int Rpt
KVNIDANGNAIPSGELKDD O50190 298 50 -
KVNIDANGNAIPSGELKDD O52147 298 50 -
KVNIDENGNAIPSGELKDD Q47014 287 50 -
KVNIDENGNAIPSGELIDD O85508 287 50 -
KVSIDEIGNSIPSGELKDD O68258 298 50 -
KVNIDELGNAIPSGVLKDD O85506 295 50 -

Motif 6 width=21
Element Seqn Id St Int Rpt
YEDQHARRQEELQLSSGIGYG O50190 347 30 -
YEDQHARRQEELQLSSGIGYG O52147 347 30 -
YDEQHAKREQEMSLSSGVGYG Q47014 336 30 -
YDEQHAKREQEMSLSSGVGYG O85508 336 30 -
YDTQYARRQEELELSSGIGYS O68258 347 30 -
YDEQQAKRQEELKVSSGAGYG O85506 344 30 -

Motif 7 width=21
Element Seqn Id St Int Rpt
GGIGAGVTTALHRRNQPAEQT O50190 376 8 -
GGIGAGVTTALHRRNQPAEQT O52147 376 8 -
GGIGAGVTAALHRKNQPAEQT Q47014 365 8 -
GGIGAGVTAALHRKNQPAEQT O85508 365 8 -
GGIGAGVTTALHRRNQPAEQT O68258 376 8 -
GGIGVAVTAALHRKNQPVEQT O85506 373 8 -

Motif 8 width=18
Element Seqn Id St Int Rpt
PVTGRLIGTPGQGIQSTY O50190 493 96 -
PVTGRLIGTPGQGIQSTY O52147 494 97 -
PVTGRLVGTPGQGIQSTY Q47014 482 96 -
PVTGRLVGTPGQGIQSTY O85508 482 96 -
QVTGRLMGTPGQGIQSTY O68258 494 97 -
DNGARLLGNPSAGIQSTY O85506 502 108 -

Motif 9 width=15
Element Seqn Id St Int Rpt
GGLRLGMGGLTSGGE O50190 517 6 -
GGLRLGMGGLTSGGE O52147 518 6 -
GGLRLGMGGLTGGGE Q47014 506 6 -
GGLRLGMGGLTGGGE O85508 506 6 -
AGLRLGMGGLTGSGG O68258 519 7 -
GGLRHDMGGLTGGSN O85506 526 6 -