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PR01368

Identifier
SYNAPSIN  [View Relations]  [View Alignment]  
Accession
PR01368
No. of Motifs
6
Creation Date
17-AUG-2000
Title
Synapsin signature
Database References

PROSITE; PS00415 SYNAPSIN_1; PS00416 SYNAPSIN_2
BLOCKS; IPB001359
PFAM; PF02078 Synapsin
INTERPRO; IPR001359
Literature References
1. NOMURA, M., UETA, Y., SERINO, R., YAMAMOTO, Y., SHIBUYA, I. AND
YAMASHITA, H.
Upregulation of synapsin IIa and IIb mRNAs in the paraventricular and
supraoptic nuclei in chronic salt loaded and lactating rats.
NEUROSCI.RES. 37 201-210 (2000).
 
2. LLINAS, R., MCGUINNESS, T., LEONARD, C.S., SUGIMORI, M. AND GREENGARD, P.
Intraterminal injection of synapsin I or calcium/calmodulin-dependent
protein kinase II alters neurotransmitter release at the squid giant
synapse.
PROC.NATL.ACAD.SCI.U.S.A. 82 3035-3039 (1985).
 
3. ROSAHL, T.W., SPILLANE, D., MISSLER, M., HERZ, J., SELIG, D.K.,
WOLFF, J.R., HAMMER, R.E., MALENKA, R.C. AND SUDHOF, T.C.
Essential functions of synapsins I and II in synaptic vesicle regulation.
NATURE 375 488-497 (1995).
 
4. FERREIRA, A., KAO, H-T., FENG, J., RAPOPORT, M. AND GREENGARD, P.
Synapsin III: developmental expression, subcellular localization, and role
in axon formation.
J.NEUROSCIENCE 20 3736-3744 (2000).

Documentation
The synapsins are a family of neuron-specific phosphoproteins that coat
synaptic vesicles and are involved in the binding between these vesicles
and the cytoskeleton (including actin filaments). The family comprises 5
homologous proteins Ia, Ib, IIa, IIb and III. Synapsins I, II, and III are
encoded by 3 different genes. The a and b isoforms of synapsin I and II are
splice variants of the primary transcripts [1].
 
Synapsin I is mainly associated with regulation of neurotransmitter release
from presynaptic neuron terminals [2]. Synapsin II, as well as being
involved in neurotransmitter release, has a role in the synaptogenesis and
synaptic plasticity responsible for long term potentiation [3]. Recent 
studies implicate synapsin III with a developmental role in neurite 
elongation and synapse formation that is distinct from the functions of
synapsins I and II [4].
 
Structurally, synapsins are multidomain proteins, of which 3 domains are
common to all the mammalian forms. The N-terminal `A' domain is ~30 residues
long and contains a serine residue that serves as an acceptor site for 
protein kinase-mediated phosphorylation. This is followed by the `B' linker
domain, which is ~80 residues long and is relatively poorly conserved.
Domain `C' is the longest, spanning approximately 300 residues. This domain
is highly conserved across all the synapsins (including those from
Drosophila) and is possessed by all splice variants. The remaining six
domains, D-I, are not shared by all the synapsins and differ both between
the primary transcripts and the splice variants.
 
SYNAPSIN is a 6-element fingerprint that provides a signature for the
synapsins. The fingerprint was derived from an initial alignment of 7 
sequences: the motifs were drawn from conserved regions spanning the `C'
domain, towards the central portion of the alignment. Two iterations on
SPTR37_10f were required to reach convergence, at which point a true set
comprising 10 sequences was identified.
Summary Information
10 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6101010101010
5000000
4000000
3000000
2000000
123456
True Positives
O14994        O70441        O88935        Q24546        
Q63537 Q92777 Q9Z1H0 SYN1_BOVIN
SYN1_HUMAN SYN1_RAT
Sequence Titles
O14994      SYNAPSIN IIIA - HOMO SAPIENS (HUMAN).         
O70441 SYNAPSIN IIIA - RATTUS NORVEGICUS (RAT).
O88935 SYNAPSIN IB - MUS MUSCULUS (MOUSE).
Q24546 HOMOLOGOUS TO SYNAPSIN I - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q63537 SYNAPSIN 2A - RATTUS NORVEGICUS (RAT).
Q92777 SYNAPSINS IIA AND IIB - HOMO SAPIENS (HUMAN).
Q9Z1H0 SYNAPSIN 2B - RATTUS NORVEGICUS (RAT).
SYN1_BOVIN SYNAPSINS IA AND IB - BOS TAURUS (BOVINE).
SYN1_HUMAN SYNAPSINS IA AND IB (BRAIN PROTEIN 4.1) - HOMO SAPIENS (HUMAN).
SYN1_RAT SYNAPSINS IA AND IB - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
DWAKYFKGKK SYN1_HUMAN 125 125 -
DWAKYFKGKK SYN1_RAT 125 125 -
DWAKCFRGKK Q92777 125 125 -
DWAKCFRGKK Q63537 126 126 -
DWSKYFHGKK O14994 103 103 -
DWSKYFHGKK O70441 103 103 -
DWSKYFRGRR Q24546 101 101 -

Motif 2 width=13
Element Seqn Id St Int Rpt
DIKVEQAEFSDLN SYN1_HUMAN 140 5 -
DIKVEQAEFSDLN SYN1_RAT 140 5 -
DIKVEQAEFSELN Q92777 140 5 -
DIKVEQAEFSELN Q63537 141 5 -
EIRVEQAEFSELN O14994 118 5 -
EIRVEQAEFSELN O70441 118 5 -
DIRGEQAEFRDIT Q24546 116 5 -

Motif 3 width=12
Element Seqn Id St Int Rpt
RSLKPDFVLIRQ SYN1_HUMAN 176 23 -
RSLKPDFVLIRQ SYN1_RAT 176 23 -
RSFRPDFVLIRQ Q92777 176 23 -
RSFRPDFVLIRQ Q63537 177 23 -
RSFKPDFILVRQ O14994 155 24 -
RSFKPDFILVRQ O70441 154 23 -
RSFRPDFVLIRQ Q24546 152 23 -

Motif 4 width=13
Element Seqn Id St Int Rpt
VQKIGQNYKAYMR SYN1_HUMAN 316 128 -
VQKIGQNYKAYMR SYN1_RAT 316 128 -
VQKIGNNYKAYMR Q92777 316 128 -
VQKIGNNYKAYMR Q63537 317 128 -
IQKIGSNYKAYMR O14994 295 128 -
IQKIGSNYKAYMR O70441 294 128 -
IQKIGNNYKAFMR Q24546 294 130 -

Motif 5 width=17
Element Seqn Id St Int Rpt
SVSGNWKTNTGSAMLEQ SYN1_HUMAN 330 1 -
SVSGNWKTNTGSAMLEQ SYN1_RAT 330 1 -
SISGNWKTNTGSAMLEQ Q92777 330 1 -
SISGNWKTNTGSAMLEQ Q63537 331 1 -
SISGNWKANTGSAMLEQ O14994 309 1 -
SISGNWKANTGSAMLEQ O70441 308 1 -
SITGNWKTNQGSAMLEQ Q24546 308 1 -

Motif 6 width=22
Element Seqn Id St Int Rpt
MSDRYKLWVDTCSEIFGGLDIC SYN1_HUMAN 349 2 -
MSDRYKLWVDTCSEIFGGLDIC SYN1_RAT 349 2 -
MSDRYKLWVDTCSEMFGGLDIC Q92777 349 2 -
MSDRYKLWVDACSEMFGGLDIC Q63537 350 2 -
MTERYRLWVDSCSEMFGGLDIC O14994 328 2 -
MTERYRLWVDSCSEMFGGLDIC O70441 327 2 -
LTEKYKSWVDEISELFGGMEVC Q24546 327 2 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
DWAKYFKGKK O88935 125 125 -
DWAKYFKGKK SYN1_HUMAN 125 125 -
DWAKYFKGKK SYN1_RAT 125 125 -
DWAKYFKGKK SYN1_BOVIN 125 125 -
DWAKCFRGKK Q92777 125 125 -
DWAKCFRGKK Q63537 126 126 -
DWAKCFRGKK Q9Z1H0 126 126 -
DWSKYFHGKK O14994 103 103 -
DWSKYFHGKK O70441 103 103 -
DWSKYFRGRR Q24546 101 101 -

Motif 2 width=13
Element Seqn Id St Int Rpt
DIKVEQAEFSDLN O88935 140 5 -
DIKVEQAEFSDLN SYN1_HUMAN 140 5 -
DIKVEQAEFSDLN SYN1_RAT 140 5 -
DIKVEQAEFSDLN SYN1_BOVIN 140 5 -
DIKVEQAEFSELN Q92777 140 5 -
DIKVEQAEFSELN Q63537 141 5 -
DIKVEQAEFSELN Q9Z1H0 141 5 -
EIRVEQAEFSELN O14994 118 5 -
EIRVEQAEFSELN O70441 118 5 -
DIRGEQAEFRDIT Q24546 116 5 -

Motif 3 width=12
Element Seqn Id St Int Rpt
RSLKPDFVLIRQ O88935 176 23 -
RSLKPDFVLIRQ SYN1_HUMAN 176 23 -
RSLKPDFVLIRQ SYN1_RAT 176 23 -
RSLKPDFVLIRQ SYN1_BOVIN 176 23 -
RSFRPDFVLIRQ Q92777 176 23 -
RSFRPDFVLIRQ Q63537 177 23 -
RSFRPDFVLIRQ Q9Z1H0 177 23 -
RSFKPDFILVRQ O14994 155 24 -
RSFKPDFILVRQ O70441 154 23 -
RSFRPDFVLIRQ Q24546 152 23 -

Motif 4 width=13
Element Seqn Id St Int Rpt
VQKIGQNYKAYMR O88935 316 128 -
VQKIGQNYKAYMR SYN1_HUMAN 316 128 -
VQKIGQNYKAYMR SYN1_RAT 316 128 -
IQKIGQNYKAYMR SYN1_BOVIN 316 128 -
VQKIGNNYKAYMR Q92777 316 128 -
VQKIGNNYKAYMR Q63537 317 128 -
VQKIGNNYKAYMR Q9Z1H0 317 128 -
IQKIGSNYKAYMR O14994 295 128 -
IQKIGSNYKAYMR O70441 294 128 -
IQKIGNNYKAFMR Q24546 294 130 -

Motif 5 width=17
Element Seqn Id St Int Rpt
SVSGNWKTNTGSAMLEQ O88935 330 1 -
SVSGNWKTNTGSAMLEQ SYN1_HUMAN 330 1 -
SVSGNWKTNTGSAMLEQ SYN1_RAT 330 1 -
SVSGNWKTNTGSAMLEQ SYN1_BOVIN 330 1 -
SISGNWKTNTGSAMLEQ Q92777 330 1 -
SISGNWKTNTGSAMLEQ Q63537 331 1 -
SISGNWKTNTGSAMLEQ Q9Z1H0 331 1 -
SISGNWKANTGSAMLEQ O14994 309 1 -
SISGNWKANTGSAMLEQ O70441 308 1 -
SITGNWKTNQGSAMLEQ Q24546 308 1 -

Motif 6 width=22
Element Seqn Id St Int Rpt
MSDRYKLWVDTCSEIFGGLDIC O88935 349 2 -
MSDRYKLWVDTCSEIFGGLDIC SYN1_HUMAN 349 2 -
MSDRYKLWVDTCSEIFGGLDIC SYN1_RAT 349 2 -
MSDRYKLWVDTCSEIFGGLDIC SYN1_BOVIN 349 2 -
MSDRYKLWVDTCSEMFGGLDIC Q92777 349 2 -
MSDRYKLWVDACSEMFGGLDIC Q63537 350 2 -
MSDRYKLWVDACSEMFGGLDIC Q9Z1H0 350 2 -
MTERYRLWVDSCSEMFGGLDIC O14994 328 2 -
MTERYRLWVDSCSEMFGGLDIC O70441 327 2 -
LTEKYKSWVDEISELFGGMEVC Q24546 327 2 -