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PR01358

Identifier
INTRLEUKIN1A  [View Relations]  [View Alignment]  
Accession
PR01358
No. of Motifs
10
Creation Date
13-MAY-2000
Title
Interleukin-1 alpha precursor signature
Database References
PRINTS; PR00262 IL1HBGF; PR00264 INTERLEUKIN1; PR01357 INTRLEUKN1AB

PDB; 1ITA
SCOP; 1ITA
CATH; 1ITA
Literature References
1. OPPENHEIM, J.J., KOVACS, E.J., MATSUSHIMA, K. AND DURUM, S.K. 
There is more than one interleukin 1. 
IMMUNOL.TODAY 7 45-56 (1986).
 
2. ROTHWELL, N.J. AND LUHESHI, G.N.
Interleukin-1 in the brain: biology, pathology and therapeutic target.
TRENDS NEUROSCIENCE 23(12) 618-625 (2000).
 
3. PRIESTLE, J.P., SCHAR, H.-P. AND GRUTTER, M.G. 
Crystallographic refinement of interleukin 1 beta at 2.0 angstrom 
resolution. 
PROC.NATL.ACAD.SCI.U.S.A. 86 9667-9671 (1989). 
 
4. MURZIN, A.G., LESK, A.M. AND CHOTHIA, C. 
Patterns of structure and sequence in the Kunitz inhibitors, interleukins-1
beta and 1 alpha and fibroblast growth factors. 
J.MOL.BIOL. 223 531-543 (1992). 

Documentation
Interleukin-1 (IL-1) is a cytokine with a wide range of biological and
physiological effects, including fever, prostaglandin synthesis (in e.g.,
fibroblasts, muscle and endothelial cells), T-lymphocyte activation, and 
interleukin-2 production [1]. This family is a member of a superfamily that
also contains the heparin binding growth factors (HBGF), the Kunitz-type 
soybean trypsin inhibitors (STI) and histactophilin. All have very similar
structures, but although the interleukin-1 and HBGF families share some
sequence similarity (about 25%), they show none at all to the STIs.
 
Over a hundred cytokines have now been identified, including several
putative new members of the IL-1 family [2]. The IL-1 family consists of 2
main classes, designated alpha (IL1A) and beta (IL1B), as well as the more
recently discovered IL-1 receptor antagonist (IL1RA). Sequence similarity 
is high within the IL1A and IL1B subfamilies (about 60-70%) but low between
them (less than 30%). IL1As and IL1Bs are synthesised as larger precursors, 
which are processed to give mature carboxy fragments. IL1B requires this 
cleavage to become biologically active, but IL1A precursor is already 
active. Both IL1A and IL1B bind to the same IL1-specific receptor on the 
target cell, which is then internalised to initiate the relevant effects
(which appear to be similar or identical).
 
The crystal structures of IL1A and IL1B [3] have been solved, showing them 
to share the same 12-stranded beta-sheet structure as both the heparin
binding growth factors and the Kunitz-type soybean trypsin inhibitors [4].
The beta-sheets are arranged in 3 similar lobes around a central axis, 6
strands forming an anti-parallel beta-barrel. Several regions, especially 
the loop between strands 4 and 5, have been implicated in receptor binding.
 
INTRLEUKIN1A is a 10-element fingerprint that provides a signature for the
interleukin-1 alpha precursor. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved regions
spanning the full alignment length - motifs 1-4 reside in the propeptide;
motif 5 encodes the N-terminus of beta-strand 1; motif 6 spans the 
C-terminus of strand 1 and the N-terminus of strand 2; motif 7 includes
strand 4; motif 8 encodes strands 6-8; motif 9 spans alpha-helix 1 and
strands 9 and 10; and motif 10 encompasses strands 11-13. Two iterations on
SPTR37_10f were required to reach convergence, at which point a true set 
comprising 14 sequences was identified. A single partial match was also
found, Q62161, a murine interleukin-1 sequence that fails to match the
N-terminal motifs.
Summary Information
  14 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
1014141414141414141414
90000000000
80000000000
70000000000
60000111111
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
IL1A_BOVIN    IL1A_CANFA    IL1A_CAPHI    IL1A_CERTO    
IL1A_FELCA IL1A_HORSE IL1A_HUMAN IL1A_MACFA
IL1A_MACMU IL1A_MOUSE IL1A_PIG IL1A_RABIT
IL1A_RAT IL1A_SHEEP
True Positive Partials
Codes involving 6 elements
Q62161
Sequence Titles
IL1A_BOVIN  INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - BOS TAURUS (BOVINE). 
IL1A_CANFA INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - CANIS FAMILIARIS (DOG).
IL1A_CAPHI INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - CAPRA HIRCUS (GOAT).
IL1A_CERTO INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - CERCOCEBUS TORQUATUS ATYS (RED-CROWNED MANGABEY) (SOOTY MANGABEY).
IL1A_FELCA INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - FELIS SILVESTRIS CATUS (CAT).
IL1A_HORSE INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - EQUUS CABALLUS (HORSE).
IL1A_HUMAN INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) (HEMATOPOIETIN-1) - HOMO SAPIENS (HUMAN).
IL1A_MACFA INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) (HEMATOPOIETIN-1) - MACACA FASCICULARIS (CRAB EATING MACAQUE) (CYNOMOLGUS MONKEY).
IL1A_MACMU INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) (HEMATOPOIETIN-1) - MACACA MULATTA (RHESUS MACAQUE).
IL1A_MOUSE INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - MUS MUSCULUS (MOUSE).
IL1A_PIG INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - SUS SCROFA (PIG).
IL1A_RABIT INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - ORYCTOLAGUS CUNICULUS (RABBIT).
IL1A_RAT INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - RATTUS NORVEGICUS (RAT).
IL1A_SHEEP INTERLEUKIN-1 ALPHA PRECURSOR (IL-1 ALPHA) - OVIS ARIES (SHEEP).

Q62161 INTERLEUKIN-1 - MUS MUSCULUS (MOUSE).
Scan History
SPTR37_10f 2  50   NSINGLE    
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
KVPDMFEDLKNCYSENEEDSS IL1A_HUMAN 3 3 -
KVPDLFEDLKNCYSENEEYSS IL1A_PIG 3 3 -
KVPDLFEDLKNCYSENEEYSS IL1A_RAT 3 3 -
KVPDLFEDLKNCYSENEDYSS IL1A_MOUSE 3 3 -

Motif 2 width=13
Element Seqn Id St Int Rpt
IDHLSLNQKSFYH IL1A_HUMAN 25 1 -
IDHLSLNQKSFYD IL1A_PIG 25 1 -
IDHLSLNQKSFYD IL1A_RAT 25 1 -
IDHLSLNQKSFYD IL1A_MOUSE 25 1 -

Motif 3 width=17
Element Seqn Id St Int Rpt
LSISETSKTSKLTFKES IL1A_HUMAN 54 16 -
LSTSKTSKTSRLNFKDS IL1A_PIG 54 16 -
LRTSETSKMSTFTFKES IL1A_RAT 54 16 -
LRTSETSKMSNFTFKES IL1A_MOUSE 54 16 -

Motif 4 width=23
Element Seqn Id St Int Rpt
GKVLKKRRLSLSQSITDDDLEAI IL1A_HUMAN 78 7 -
GKILKKRRLSLNQFITDDDLEAI IL1A_PIG 78 7 -
GKILKKRRLSFNQPFTEDDLEAI IL1A_RAT 81 10 -
GKILKKRRLSFSETFTEDDLQSI IL1A_MOUSE 81 10 -

Motif 5 width=18
Element Seqn Id St Int Rpt
PRSAPFSFLSNVKYNFMR IL1A_HUMAN 111 10 -
PRSATYSFQSNMKYNFMR IL1A_PIG 111 10 -
PRSAPHSFQNNLRYKLIR IL1A_RAT 113 9 -
PRSAPYTYQSDLRYKLMK IL1A_MOUSE 113 9 -

Motif 6 width=17
Element Seqn Id St Int Rpt
RIIKYEFILNDALNQSI IL1A_HUMAN 128 -1 -
RVINHQCILNDARNQSI IL1A_PIG 128 -1 -
RIVKQEFIMNDSLNQNI IL1A_RAT 130 -1 -
KLVRQKFVMNDSLNQTI IL1A_MOUSE 130 -1 -

Motif 7 width=17
Element Seqn Id St Int Rpt
LHNLDEAVKFDMGAYKS IL1A_HUMAN 157 12 -
LNNLDEAVKFDMAAYTS IL1A_PIG 158 13 -
LNDLQLEVKFDMYAYSS IL1A_RAT 160 13 -
LNDLQQEVKFDMYAYSS IL1A_MOUSE 160 13 -

Motif 8 width=24
Element Seqn Id St Int Rpt
LYVTAQDEDQPVLLKEMPEIPKTI IL1A_HUMAN 191 17 -
LFVSAQNEDEPVLLKELPETPKTI IL1A_PIG 191 16 -
LFVSAQGEDKPVLLKEIPETPKLI IL1A_RAT 194 17 -
LFVSAQGEDQPVLLKELPETPKLI IL1A_MOUSE 194 17 -

Motif 9 width=21
Element Seqn Id St Int Rpt
ETNLLFFWETHGTKNYFTSVA IL1A_HUMAN 218 3 -
ETSLLFFWEKHGNMDYFKSAA IL1A_PIG 217 2 -
ETDLIFFWEKINSKNYFTSAA IL1A_RAT 221 3 -
ETDLIFFWKSINSKNYFTSAA IL1A_MOUSE 221 3 -

Motif 10 width=26
Element Seqn Id St Int Rpt
PNLFIATKQDYWVCLAGGPPSITDFQ IL1A_HUMAN 240 1 -
PKLFIATRQEKLVHMAPGLPSVTDFQ IL1A_PIG 239 1 -
PELLIATKEQSQVHLARGLPSMIDFQ IL1A_RAT 243 1 -
PELFIATKEQSRVHLARGLPSMTDFQ IL1A_MOUSE 243 1 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
KVPDLFEDLKNCYSENEDYSS IL1A_BOVIN 3 3 -
KVPDLFEDLKNCYSENEDYSS IL1A_CAPHI 3 3 -
KVPDLFEDLKNCYSENEDYSS IL1A_SHEEP 3 3 -
KVPDMFEDLKNCYSENEEDSS IL1A_HUMAN 3 3 -
KVPDLFEDLKNCYSENEDYSS IL1A_HORSE 3 3 -
KVPDLFEDLKNCYSENEEYSS IL1A_CANFA 3 3 -
KVPDLFEDLKNCYSENEEYSS IL1A_PIG 3 3 -
KVPDMFEDLKNCYSENEEVSS IL1A_MACFA 3 3 -
KVPDMFEDLKNCYSENEEDSS IL1A_MACMU 3 3 -
KVPDMFEDLKNCYSENEEDSS IL1A_CERTO 3 3 -
KVPDLFEDLKNCYSENEEYSS IL1A_FELCA 3 3 -
KVPDLFEDLKNCYSENEEYSS IL1A_RAT 3 3 -
KVPDLFEDLKNCFSENEEYSS IL1A_RABIT 3 3 -
KVPDLFEDLKNCYSENEDYSS IL1A_MOUSE 3 3 -

Motif 2 width=13
Element Seqn Id St Int Rpt
IDHLSLNQKSFYD IL1A_BOVIN 25 1 -
IDHLSLNQKSFYD IL1A_CAPHI 25 1 -
IDHLSLNQKSFYD IL1A_SHEEP 25 1 -
IDHLSLNQKSFYH IL1A_HUMAN 25 1 -
IDHLSLTQKSFYD IL1A_HORSE 25 1 -
IDHLSLNQKSFYD IL1A_CANFA 25 1 -
IDHLSLNQKSFYD IL1A_PIG 25 1 -
IDHLSLNQKSFYD IL1A_MACFA 25 1 -
IDHLSLNQKSFYD IL1A_MACMU 25 1 -
IDHLFLNQKSFYD IL1A_CERTO 25 1 -
IDHLTLNQKSFYD IL1A_FELCA 25 1 -
IDHLSLNQKSFYD IL1A_RAT 25 1 -
IDHLSLNQKSFYD IL1A_RABIT 25 1 -
IDHLSLNQKSFYD IL1A_MOUSE 25 1 -

Motif 3 width=17
Element Seqn Id St Int Rpt
LDTSETSKTSKLSFKEN IL1A_BOVIN 54 16 -
LDTSETSKTSRLSFKEN IL1A_CAPHI 54 16 -
LDTSETSKTSRLSFKEN IL1A_SHEEP 54 16 -
LSISETSKTSKLTFKES IL1A_HUMAN 54 16 -
LSTSETSKTSKLNFKES IL1A_HORSE 54 16 -
LSTSEISKTSQLTFKEN IL1A_CANFA 50 12 -
LSTSKTSKTSRLNFKDS IL1A_PIG 54 16 -
LSISEISKTSKLTFKQS IL1A_MACFA 54 16 -
LSISEISKTSKLTFKQS IL1A_MACMU 54 16 -
LSISEISKTSKLSFKQS IL1A_CERTO 54 16 -
PSTSETSKTPQLTLKKS IL1A_FELCA 54 16 -
LRTSETSKMSTFTFKES IL1A_RAT 54 16 -
LSTSETSVSPNLTFQEN IL1A_RABIT 54 16 -
LRTSETSKMSNFTFKES IL1A_MOUSE 54 16 -

Motif 4 width=23
Element Seqn Id St Int Rpt
GKILKKRRLSLNQFITDDDLEAI IL1A_BOVIN 78 7 -
GKILKKRRLSLNQFITDDDLEAI IL1A_CAPHI 78 7 -
GKILKKRRLSLNQFITDDDLEAI IL1A_SHEEP 78 7 -
GKVLKKRRLSLSQSITDDDLEAI IL1A_HUMAN 78 7 -
GKTLKKRRLSLNQFITNDDLEAI IL1A_HORSE 78 7 -
GKILKKRRLSLSQFITDDDLEGI IL1A_CANFA 74 7 -
GKILKKRRLSLNQFITDDDLEAI IL1A_PIG 78 7 -
GKVLKKRRLSLSQSITDNNLEAI IL1A_MACFA 78 7 -
GKVLKKRRLSLSQSITDNNLEAI IL1A_MACMU 78 7 -
GKVLKKRRLSLSQSIADDNLEAI IL1A_CERTO 78 7 -
GKILKKRRLSLNQFLTADDLEAI IL1A_FELCA 78 7 -
GKILKKRRLSFNQPFTEDDLEAI IL1A_RAT 81 10 -
GKILKKRRLSLNQPITDVDLETN IL1A_RABIT 78 7 -
GKILKKRRLSFSETFTEDDLQSI IL1A_MOUSE 81 10 -

Motif 5 width=18
Element Seqn Id St Int Rpt
PRSAHYSFQSNVKYNFMR IL1A_BOVIN 111 10 -
PRSAHYSFQSNVKYNFMR IL1A_CAPHI 111 10 -
PRSAHYSFQSNVKYNFMR IL1A_SHEEP 111 10 -
PRSAPFSFLSNVKYNFMR IL1A_HUMAN 111 10 -
PRSVHYNFQSNTKYNFMR IL1A_HORSE 111 10 -
PRSVAYNFHNNEKYNYIR IL1A_CANFA 107 10 -
PRSATYSFQSNMKYNFMR IL1A_PIG 111 10 -
PRSAPFSFLSNMTYHFIR IL1A_MACFA 111 10 -
PRSAPFSFLSNMTYHFIR IL1A_MACMU 111 10 -
PRSAPFSFLSNMTYHIIR IL1A_CERTO 111 10 -
PRSVAPNFYSSEKYNYQK IL1A_FELCA 111 10 -
PRSAPHSFQNNLRYKLIR IL1A_RAT 113 9 -
PRSVPYTFQRNMRYKYLR IL1A_RABIT 111 10 -
PRSAPYTYQSDLRYKLMK IL1A_MOUSE 113 9 -

Motif 6 width=17
Element Seqn Id St Int Rpt
RVIHQECILNDALNQSI IL1A_BOVIN 128 -1 -
RVIHQECILNDALNQSI IL1A_CAPHI 128 -1 -
RVIHQECILNDALNQSI IL1A_SHEEP 128 -1 -
RIIKYEFILNDALNQSI IL1A_HUMAN 128 -1 -
RIVNHQCTLNDALNQSV IL1A_HORSE 128 -1 -
RIIKSQFILNDNLNQSI IL1A_CANFA 124 -1 -
RVINHQCILNDARNQSI IL1A_PIG 128 -1 -
RIIKHEFILNDTLNQTI IL1A_MACFA 128 -1 -
RIIKHEFILNDTLNQTI IL1A_MACMU 128 -1 -
RIIKHESILNDTLNQTI IL1A_CERTO 128 -1 -
KIIKSQFILNDNLSQSV IL1A_FELCA 128 -1 -
RIVKQEFIMNDSLNQNI IL1A_RAT 130 -1 -
RIIKQEFTLNDALNQSL IL1A_RABIT 128 -1 -
KLVRQKFVMNDSLNQTI IL1A_MOUSE 130 -1 -

Motif 7 width=17
Element Seqn Id St Int Rpt
LNNLEEAVKFDMVAYVS IL1A_BOVIN 158 13 -
LNNLEEAVKFDMVAYVS IL1A_CAPHI 158 13 -
LNNLEEAVKFDMVAYVS IL1A_SHEEP 158 13 -
LHNLDEAVKFDMGAYKS IL1A_HUMAN 157 12 -
LNNLDDAVKFDMGAYTS IL1A_HORSE 158 13 -
LQNLDDAVKFDMGAYTS IL1A_CANFA 154 13 -
LNNLDEAVKFDMAAYTS IL1A_PIG 158 13 -
IHNLDEAVKFDMGAYTS IL1A_MACFA 157 12 -
IHNLDEAVKFDMGAYTS IL1A_MACMU 157 12 -
IHNLDEAVKFDMGAYTS IL1A_CERTO 157 12 -
LQNLDDAVKFDMGAYTS IL1A_FELCA 158 13 -
LNDLQLEVKFDMYAYSS IL1A_RAT 160 13 -
LQNLGDAVKFDMGVYMT IL1A_RABIT 158 13 -
LNDLQQEVKFDMYAYSS IL1A_MOUSE 160 13 -

Motif 8 width=24
Element Seqn Id St Int Rpt
LFVSAQNEDEPVLLKEMPETPKII IL1A_BOVIN 191 16 -
LFVSAQNEDEPVLLKEMPETPKII IL1A_CAPHI 191 16 -
LFVSAQNEDEPVLHKEMPETPKII IL1A_SHEEP 191 16 -
LYVTAQDEDQPVLLKEMPEIPKTI IL1A_HUMAN 191 17 -
LFVSAQNEDEPVLLKEMPDTPKTI IL1A_HORSE 191 16 -
LFVSAQNEDEPVLLKEMPETPKTI IL1A_CANFA 186 15 -
LFVSAQNEDEPVLLKELPETPKTI IL1A_PIG 191 16 -
LYVSAQDEDQPVLLKEMPEIPKTI IL1A_MACFA 191 17 -
LYVSAQDEDQPVLLKEMPEINKTI IL1A_MACMU 191 17 -
LYVSAQDEDQPVLLKEMPEIPKTT IL1A_CERTO 191 17 -
LFVSAQNEDEPVLLKEMPETPKTI IL1A_FELCA 191 16 -
LFVSAQGEDKPVLLKEIPETPKLI IL1A_RAT 194 17 -
LFVSAQNEDEPVLLKEMPETPRII IL1A_RABIT 191 16 -
LFVSAQGEDQPVLLKELPETPKLI IL1A_MOUSE 194 17 -

Motif 9 width=21
Element Seqn Id St Int Rpt
ETNLLFFWEKHGSMDYFKSVA IL1A_BOVIN 217 2 -
ETNLLFFWEKHGSMDYFKSVA IL1A_CAPHI 217 2 -
ETNLLFFWEKHGSMDYFKSVA IL1A_SHEEP 217 2 -
ETNLLFFWETHGTKNYFTSVA IL1A_HUMAN 218 3 -
ETNLLFFWERHGSKNYFKSVA IL1A_HORSE 217 2 -
ETNLLFFWERHGSKHYFKSVA IL1A_CANFA 212 2 -
ETSLLFFWEKHGNMDYFKSAA IL1A_PIG 217 2 -
ETNFLFFWETHGTKNYFISVA IL1A_MACFA 218 3 -
ETNFLFFWETHGTKNYFISVA IL1A_MACMU 218 3 -
ETNSLSSWETRGTKNYFISVA IL1A_CERTO 218 3 -
ETNLLFFWERHGSKNYFKSVA IL1A_FELCA 217 2 -
ETDLIFFWEKINSKNYFTSAA IL1A_RAT 221 3 -
ESDILFFWETQGNKNYFKSAA IL1A_RABIT 218 3 -
ETDLIFFWKSINSKNYFTSAA IL1A_MOUSE 221 3 -

Motif 10 width=26
Element Seqn Id St Int Rpt
PKLFIATKQEKLVHMASGPPSITDFQ IL1A_BOVIN 239 1 -
PKLFIATKQEKLVHMASGPPSITDFQ IL1A_CAPHI 239 1 -
PKLFIATKQEKLVHMASGPPSITDFL IL1A_SHEEP 239 1 -
PNLFIATKQDYWVCLAGGPPSITDFQ IL1A_HUMAN 240 1 -
PKLFIATKQGKLVHMARGQPSITDFQ IL1A_HORSE 239 1 -
PKLFIATQERKLVHMARGQPSITDFR IL1A_CANFA 234 1 -
PKLFIATRQEKLVHMAPGLPSVTDFQ IL1A_PIG 239 1 -
PNLFIATKHDNWVCLAKGLPSITDFQ IL1A_MACFA 240 1 -
PNLFIATKHDNWVCLAKGLPSITDFQ IL1A_MACMU 240 1 -
PNLFIATKHDNWVCLAKGLPSITDFQ IL1A_CERTO 240 1 -
PKLFIATQEEQLVHMARGLPSVTDFQ IL1A_FELCA 239 1 -
PELLIATKEQSQVHLARGLPSMIDFQ IL1A_RAT 243 1 -
PQLFIATKPEHLVHMARGLPSMTDFQ IL1A_RABIT 240 1 -
PELFIATKEQSRVHLARGLPSMTDFQ IL1A_MOUSE 243 1 -