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PR01347

Identifier
EPHRIN  [View Relations]  [View Alignment]  
Accession
PR01347
No. of Motifs
5
Creation Date
11-MAY-2000
Title
Ephrin signature
Database References

PROSITE; PS01299 EPHRIN
BLOCKS; BL01299
PFAM; PF00812 Ephrin
INTERPRO; IPR001799
Literature References
1. NAKAMOTO, M.
Eph Receptors and Ephrins.
INT.J.BIOCHEM.CELL BIOL. 32 7-12 (2000).
 
2. WILKINSON, D.G.
Eph Receptors and Ephrins: Regulators of Guidance and Assembly.
INT.REV.CYTOL. 196 177-244 (2000).

Documentation
Ephrins are peptides that exert their effects via binding to Eph receptors,
which form the largest known family of receptor tyrosine kinases. Ephrins
and their receptors have been implicated in many developmental processes,
including neuronal network formation, the patterning of the neural tube 
and the paraxial mesoderm, the guidance of cell migration and vascular
formation. Ephrins have been shown to act as positional labels and play a
key role in axon and neural crest cell pathway finding [1]. Together
with their receptors, they are important regulators of the repulsion and
adhesion of cells involved in cellular organisation.
 
Interactions between ephrins and their receptors are far from selective,
but can generally be classified as follows: EphA receptors bind glycosyl-
phosphatidylinositol(GPI)-linkage cell membrane anchored ephrin-A ligands,
while EphB receptors bind to ephrin-B proteins that have a transmembrane 
and cytoplasmic domain. In addition, ephrin-B proteins can transduce signals
as well as the receptor, resulting in bi-directional signalling. A major 
role of ephrins and their receptors is to mediate cell-contact-dependent
repulsion. However, by contrast, ephrins can also trigger an adhesive
response in epithelial cells and are essential for the remodelling of blood
vessels. Recent evidence suggests that the cellular response is dependent
upon the extent of ephrin-receptor binding and that the actin cytoskeleton
is a primary target of the ephrin signal transduction pathway [2].
 
EPHRIN is a 5-element fingerprint that provides a signature for the ephrins.
The fingerprint was derived from an initial alignment of 6 sequences: the
motifs were drawn from conserved regions spanning the N-terminal half of 
the alignment. Two iterations on SPTR37_10f were required to reach
convergence, at which point a true set comprising 28 sequences was
identified.
Summary Information
28 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
52828282828
400000
300000
200000
12345
True Positives
EFA1_HUMAN    EFA1_MOUSE    EFA1_RAT      EFA1_XENLA    
EFA2_BRARE EFA2_CHICK EFA2_HUMAN EFA2_MOUSE
EFA3_HUMAN EFA4_HUMAN EFA4_MOUSE EFA5_BRARE
EFA5_CHICK EFA5_HUMAN EFA5_MOUSE EFA5_RAT
EFB1_CHICK EFB1_HUMAN EFB1_MOUSE EFB1_RAT
EFB1_XENLA EFB2_BRARE EFB2_HUMAN EFB2_MOUSE
EFB3_HUMAN EFB3_MOUSE O93431 O95457
Sequence Titles
EFA1_HUMAN  EPHRIN-A1 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 1) (LERK-1) (IMMEDIATE EARLY RESPONSE PROTEIN B61) (TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 4) - HOMO SAPIENS (HUMAN). 
EFA1_MOUSE EPHRIN-A1 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 1) (LERK-1) (IMMEDIATE EARLY RESPONSE PROTEIN B61) - MUS MUSCULUS (MOUSE).
EFA1_RAT EPHRIN-A1 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 1) (LERK-1) (IMMEDIATE EARLY RESPONSE PROTEIN B61) - RATTUS NORVEGICUS (RAT).
EFA1_XENLA EPHRIN-A1 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 1) (LERK-1) (XELF) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
EFA2_BRARE EPHRIN-A2 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 6) (LERK-6) (ELF-1) - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
EFA2_CHICK EPHRIN-A2 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 6) (LERK-6) (ELF-1) - GALLUS GALLUS (CHICKEN).
EFA2_HUMAN EPHRIN-A2 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 6) (LERK-6) (HEK7-LIGAND) (HEK7-L) - HOMO SAPIENS (HUMAN).
EFA2_MOUSE EPHRIN-A2 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 6) (LERK-6) (ELF-1) (CEK7-LIGAND) (CEK7-L) - MUS MUSCULUS (MOUSE).
EFA3_HUMAN EPHRIN-A3 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 3) (LERK-3) (EHK1 LIGAND) (EHK1-L) - HOMO SAPIENS (HUMAN).
EFA4_HUMAN EPHRIN-A4 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 4) (LERK-4) - HOMO SAPIENS (HUMAN).
EFA4_MOUSE EPHRIN-A4 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 4) (LERK-4) - MUS MUSCULUS (MOUSE).
EFA5_BRARE EPHRIN-A5 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 7) (LERK-7) (AL-1) - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
EFA5_CHICK EPHRIN-A5 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 7) (LERK-7) (RAGS PROTEIN) - GALLUS GALLUS (CHICKEN).
EFA5_HUMAN EPHRIN-A5 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 7) (LERK-7) (AL-1) - HOMO SAPIENS (HUMAN).
EFA5_MOUSE EPHRIN-A5 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 7) (LERK-7) (AL-1) - MUS MUSCULUS (MOUSE).
EFA5_RAT EPHRIN-A5 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 7) (LERK-7) (AL-1) - RATTUS NORVEGICUS (RAT).
EFB1_CHICK EPHRIN-B1 PRECURSOR (CEK5 LIGAND) (CEL5-L) - GALLUS GALLUS (CHICKEN).
EFB1_HUMAN EPHRIN-B1 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 2) (LERK-2) (ELK LIGAND) (ELK-L) - HOMO SAPIENS (HUMAN).
EFB1_MOUSE EPHRIN-B1 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 2) (LERK-2) (ELK LIGAND) (ELK-L) (STRA1 PROTEIN) (CEK5 RECEPTOR LIGAND) (CEK5-L) - MUS MUSCULUS (MOUSE).
EFB1_RAT EPHRIN-B1 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 2) (LERK-2) (ELK LIGAND) (ELK-L) - RATTUS NORVEGICUS (RAT).
EFB1_XENLA EPHRIN-B1 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 2) (LERK-2) (ELK LIGAND) (ELK-L) (XLERK) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
EFB2_BRARE EPHRIN-B2 PRECURSOR - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
EFB2_HUMAN EPHRIN-B2 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 5) (LERK-5) (HTK LIGAND) (HTK-L) - HOMO SAPIENS (HUMAN).
EFB2_MOUSE EPHRIN-B2 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 5) (LERK-5) (HTK LIGAND) (HTK-L) (ELF-2) - MUS MUSCULUS (MOUSE).
EFB3_HUMAN EPHRIN-B3 PRECURSOR (EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 8) (LERK-8) (EPH-RELATED RECEPTOR TRANSMEMBRANE LIGAND ELK-L3) - HOMO SAPIENS (HUMAN).
EFB3_MOUSE EPHRIN-B3 PRECURSOR - MUS MUSCULUS (MOUSE).
O93431 EPHRIN A-L1 - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
O95457 EPHRIN-A4 PROTEIN - HOMO SAPIENS (HUMAN).
Scan History
SPTR37_10f 2  32   NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
VYWNSTNPRFQQGDY EFA5_CHICK 34 34 -
VFWNRTNPRFQRGDY EFA5_BRARE 34 34 -
VFWNSSNPKFRNEDY EFA1_HUMAN 23 23 -
VFWNSSNYRFMQEDY EFA1_XENLA 33 33 -
VSWSSLNPKFLSGKG EFB1_HUMAN 35 35 -
IYWNSSNSKFLPGQG EFB2_MOUSE 36 36 -

Motif 2 width=18
Element Seqn Id St Int Rpt
HIDVCINDYLDVFCPHYE EFA5_CHICK 49 0 -
HIDVCINDYLDVYCPHYE EFA5_BRARE 49 0 -
TIHVQLNDYVDIICPHYE EFA1_HUMAN 38 0 -
TVQVQLNDYLDIVCPYYE EFA1_XENLA 48 0 -
VIYPKIGDKLDIICPRAE EFB1_HUMAN 51 1 -
VLYPQIGDKLDIICPKVD EFB2_MOUSE 52 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ERYVLYMVNFDGYSSC EFA5_CHICK 75 8 -
ERYVLYMVNYDGYSTC EFA5_BRARE 75 8 -
EQYILYLVEHEEYQLC EFA1_HUMAN 65 9 -
ERYTLFLVDYEEYETC EFA1_XENLA 75 9 -
EYYKLYLVRPEQAAAC EFB1_HUMAN 74 5 -
EYYKVYMVDKDQADRC EFB2_MOUSE 77 7 -

Motif 4 width=13
Element Seqn Id St Int Rpt
KFSEKFQLFTPFS EFA5_CHICK 113 22 -
KFSEKFQLFTPFS EFA5_BRARE 113 22 -
KLSEKFQRFTPFT EFA1_HUMAN 103 22 -
KFCEKFQKFTPFT EFA1_XENLA 113 22 -
RFTIKFQEFSPNY EFB1_HUMAN 109 19 -
KFTIKFQEFSPNL EFB2_MOUSE 112 19 -

Motif 5 width=12
Element Seqn Id St Int Rpt
LGFEFRPGREYF EFA5_CHICK 126 0 -
LGFEFRPGREYY EFA5_BRARE 126 0 -
LGKEFKEGHSYY EFA1_HUMAN 116 0 -
LGTEFREGRTYY EFA1_XENLA 126 0 -
MGLEFKKHHDYY EFB1_HUMAN 122 0 -
WGLEFQKNKDYY EFB2_MOUSE 125 0 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
VYWNRSNPRFHRGDY EFA2_CHICK 33 33 -
VYWNSSNPRFQRGDY EFA5_HUMAN 34 34 -
VYWNSSNPRFQRGDY EFA5_RAT 34 34 -
VYWNSSNPRFQRGDY EFA5_MOUSE 34 34 -
VYWNSTNPRFQQGDY EFA5_CHICK 34 34 -
VFWNRTNPRFQRGDY EFA5_BRARE 34 34 -
VYWNRSNPRFHAGAG EFA2_HUMAN 39 39 -
VYWNRSNPRFQVSAV EFA2_MOUSE 35 35 -
VYWNSSNSRFWQGEY EFA2_BRARE 29 29 -
VFWNSSNPKFREEDY EFA1_MOUSE 23 23 -
VFWNSSNPKFREEDY EFA1_RAT 23 23 -
VYWNSTNANFLWDDY O93431 25 25 -
VYWNSSNPRLLRGDA EFA4_HUMAN 30 30 -
VYWNSSNPRLLRGDA O95457 30 30 -
VFWNSSNPKFRNEDY EFA1_HUMAN 23 23 -
VFWNSSNYRFMQEDY EFA1_XENLA 33 33 -
IYWNSSNPRLLRGDA EFA4_MOUSE 30 30 -
VSWSSLNPKFLSGKG EFB1_HUMAN 35 35 -
VSWSSLNPKFLSGKG EFB1_MOUSE 35 35 -
VSWSSLNPKFLSGKG EFB1_RAT 35 35 -
VTWNSQNPRFISGKG EFB1_XENLA 28 28 -
VYWNSSNQHLRREGY EFA3_HUMAN 35 35 -
IYWNSSNSKFLPGQG EFB2_HUMAN 33 33 -
IYWNSSNSKFLPGQG EFB2_MOUSE 36 36 -
IYWNTTNTKFVPGQG EFB2_BRARE 30 30 -
VYWNSANKRFQAEGG EFB3_MOUSE 33 33 -
VYWNSANKRFQAEGG EFB3_HUMAN 33 33 -
VSWSAGNPKFMSGKG EFB1_CHICK 31 31 -

Motif 2 width=18
Element Seqn Id St Int Rpt
TVEVSINDYLDIYCPHYE EFA2_CHICK 48 0 -
HIDVCINDYLDVFCPHYE EFA5_HUMAN 49 0 -
HIDVCINDYLDVFCPHYE EFA5_RAT 49 0 -
HIDVCINDYLDVFCPHYE EFA5_MOUSE 49 0 -
HIDVCINDYLDVFCPHYE EFA5_CHICK 49 0 -
HIDVCINDYLDVYCPHYE EFA5_BRARE 49 0 -
TVEVSINDYLDIYCPHYG EFA2_HUMAN 60 6 -
TVEVSINDYLDIYCPHYG EFA2_MOUSE 56 6 -
TVAVSINDYLDVYCPYYE EFA2_BRARE 44 0 -
TVHVQLNDYLDIICPHYE EFA1_MOUSE 38 0 -
TVHVQLNDYLDIICPHYE EFA1_RAT 38 0 -
TVDVRINDYLDIICPHYA O93431 40 0 -
VVELGLNDYLDIVCPHYE EFA4_HUMAN 45 0 -
VVELGLNDYLDIVCPHYE O95457 45 0 -
TIHVQLNDYVDIICPHYE EFA1_HUMAN 38 0 -
TVQVQLNDYLDIVCPYYE EFA1_XENLA 48 0 -
VVELGFNDYLDIFCPHYE EFA4_MOUSE 45 0 -
VIYPKIGDKLDIICPRAE EFB1_HUMAN 51 1 -
VIYPKIGDKLDIICPRAE EFB1_MOUSE 51 1 -
VIYPKIGDKLDIICPRAE EFB1_RAT 51 1 -
VLYPEIGDRLDIICPKGL EFB1_XENLA 44 1 -
TVQVNVNDYLDIYCPHYN EFA3_HUMAN 50 0 -
VLYPQIGDKLDIICPKVD EFB2_HUMAN 49 1 -
VLYPQIGDKLDIICPKVD EFB2_MOUSE 52 1 -
VLYPQIGDKMDIVCPRVE EFB2_BRARE 46 1 -
VLYPQIGDRLDLLCPRAR EFB3_MOUSE 49 1 -
VLYPQIGDRLDLLCPRAR EFB3_HUMAN 49 1 -
VIYPEIGDKLDIICPKAE EFB1_CHICK 47 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ERYVLYMVNYEGHASC EFA2_CHICK 74 8 -
ERYVLYMVNFDGYSAC EFA5_HUMAN 75 8 -
ERYVLYMVNFDGYSAC EFA5_RAT 75 8 -
ERYVLYMVNFDGYSAC EFA5_MOUSE 75 8 -
ERYVLYMVNFDGYSSC EFA5_CHICK 75 8 -
ERYVLYMVNYDGYSTC EFA5_BRARE 75 8 -
EHYVLYMVNGEGHASC EFA2_HUMAN 87 9 -
ERYILYMVNGEGHASC EFA2_MOUSE 83 9 -
ERYILFMVNHDGYLTC EFA2_BRARE 70 8 -
ERYTLYMVEHQEYVAC EFA1_MOUSE 65 9 -
ERYSLYMVEHQEYVTC EFA1_RAT 65 9 -
ERYVLYMVELEDYENC O93431 67 9 -
ETFALYMVDWPGYESC EFA4_HUMAN 71 8 -
ETFALYMVDWPGYESC O95457 71 8 -
EQYILYLVEHEEYQLC EFA1_HUMAN 65 9 -
ERYTLFLVDYEEYETC EFA1_XENLA 75 9 -
ETFALYMVDWSGYEAC EFA4_MOUSE 71 8 -
EYYKLYLVRPEQAAAC EFB1_HUMAN 74 5 -
EYYKLYLVRPEQAAAC EFB1_MOUSE 74 5 -
EYYKLYLVRPEQAAAC EFB1_RAT 74 5 -
EYYKLYMVRRDQLEAC EFB1_XENLA 66 4 -
EQYVLYMVSRNGYRTC EFA3_HUMAN 84 16 -
EYYKVYMVDKDQADRC EFB2_HUMAN 74 7 -
EYYKVYMVDKDQADRC EFB2_MOUSE 77 7 -
EYYKLYMVPLEQLKSC EFB2_BRARE 71 7 -
EFYKLYLVEGAQGRRC EFB3_MOUSE 77 10 -
EFYKLYLVGGAQGRRC EFB3_HUMAN 77 10 -
DYYKLYLVKKDQADAC EFB1_CHICK 70 5 -

Motif 4 width=13
Element Seqn Id St Int Rpt
KFSEKFQLFTPFS EFA2_CHICK 112 22 -
KFSEKFQLFTPFS EFA5_HUMAN 113 22 -
KFSEKFQLFTPFS EFA5_RAT 113 22 -
KFSEKFQLFTPFS EFA5_MOUSE 113 22 -
KFSEKFQLFTPFS EFA5_CHICK 113 22 -
KFSEKFQLFTPFS EFA5_BRARE 113 22 -
KFSEKFQLFTPFS EFA2_HUMAN 125 22 -
KFSEKFQLFTPFS EFA2_MOUSE 121 22 -
RFSEKFQLFTPFS EFA2_BRARE 108 22 -
KLSEKFQRFTPFI EFA1_MOUSE 103 22 -
KLSEKFQRFTPFT EFA1_RAT 103 22 -
KFSEKFQRFTPFT O93431 105 22 -
QFSEKIQRFTPFS EFA4_HUMAN 107 20 -
QFSEKIQRFTPFS O95457 107 20 -
KLSEKFQRFTPFT EFA1_HUMAN 103 22 -
KFCEKFQKFTPFT EFA1_XENLA 113 22 -
RFSEKIQRYTPFP EFA4_MOUSE 110 23 -
RFTIKFQEFSPNY EFB1_HUMAN 109 19 -
RFTIKFQEFSPNY EFB1_MOUSE 109 19 -
RFTIKFQEFSPNY EFB1_RAT 109 19 -
RFTIKFQEFSPNY EFB1_XENLA 101 19 -
KFSEKFQRYSAFS EFA3_HUMAN 121 21 -
KFTIKFQEFSPNL EFB2_HUMAN 109 19 -
KFTIKFQEFSPNL EFB2_MOUSE 112 19 -
KFTLKFQEFSPNL EFB2_BRARE 106 19 -
RFTIKFQEYSPNL EFB3_MOUSE 112 19 -
RFTIKFQEYSPNL EFB3_HUMAN 112 19 -
RFTIKFQEFSPNY EFB1_CHICK 105 19 -

Motif 5 width=12
Element Seqn Id St Int Rpt
LGFEFRPGHEYY EFA2_CHICK 125 0 -
LGFEFRPGREYF EFA5_HUMAN 126 0 -
LGFEFRPGREYF EFA5_RAT 126 0 -
LGFEFRPGREYF EFA5_MOUSE 126 0 -
LGFEFRPGREYF EFA5_CHICK 126 0 -
LGFEFRPGREYY EFA5_BRARE 126 0 -
LGFEFRPGHEYY EFA2_HUMAN 138 0 -
LGFEFRPGHEYY EFA2_MOUSE 134 0 -
LGFEFRPGHEYY EFA2_BRARE 121 0 -
LGKEFKEGHSYY EFA1_MOUSE 116 0 -
LGKEFKEGHSYY EFA1_RAT 116 0 -
LGKEFRQGESYY O93431 118 0 -
LGFEFLPGETYY EFA4_HUMAN 120 0 -
LGFEFLPGETYY O95457 120 0 -
LGKEFKEGHSYY EFA1_HUMAN 116 0 -
LGTEFREGRTYY EFA1_XENLA 126 0 -
LGFEFLPGETYY EFA4_MOUSE 123 0 -
MGLEFKKHHDYY EFB1_HUMAN 122 0 -
MGLEFKKYHDYY EFB1_MOUSE 122 0 -
MGLEFKKYHDYY EFB1_RAT 122 0 -
MGLEFRRNQDYY EFB1_XENLA 114 0 -
LGYEFHAGHEYY EFA3_HUMAN 134 0 -
WGLEFQKNKDYY EFB2_HUMAN 122 0 -
WGLEFQKNKDYY EFB2_MOUSE 125 0 -
WGLEFFRGKDYY EFB2_BRARE 119 0 -
WGHEFRSHHDYY EFB3_MOUSE 125 0 -
WGHEFRSHHDYY EFB3_HUMAN 125 0 -
MGLEFKRQQDYF EFB1_CHICK 118 0 -