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PR01333

Identifier
2POREKCHANEL  [View Relations]  [View Alignment]  
Accession
PR01333
No. of Motifs
2
Creation Date
05-FEB-2000
Title
Two pore domain K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01095 TASKCHANNEL; PR01588 THIKCHANNEL
PRINTS; PR01691 TRAAKCHANNEL; PR01499 TREKCHANNEL; PR01586 TWIKCHANNEL
Literature References
1. KETCHUM, K.A., JOINER, W.J., SELLERS, A.J., KACZMAREK, L.K.
AND GOLDSTEIN, S.A.N.
A new family of outwardly rectifying potassium channel proteins with two
pore domains in tandem.
NATURE 376 690-695 (1995).
 
2. GOLDSTEIN, S.A.N., PRICE, L.A., ROSENTHAL, D.N. AND PAUSCH, M.H.
ORK1, a potassium-selective leak channel with two pore domains cloned from
Drosophila melanogaster by expression in Saccharomyces cerevisiae.
PROC.NATL.ACAD.SCI.U.S.A. 93 13256-13261 (1996).
 
3. FINK, M. DUPRAT, F., LESAGE, F., REYES, R., ROMEY, G., HEURTEAUX, C.
AND LAZDUNSKI, M.
Cloning, functional expression and brain localization of a novel
unconventional outward rectifier K+ channel.
EMBO J. 15 6854-6862 (1996).
 
4. LESAGE, F., GUILLEMARE, E., FINK, M., DUPRAT, F., LAZDUNSKI, M., 
ROMEY, G. AND BARHANIN, J.
TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with novel
structure.
EMBO J. 15 1004-1011 (1996).
 
5. DUPRAT, F., LESAGE, F., FINK, M., REYES, R., HEURTEAUX, C. 
AND LAZDUNSKI, M.
TASK, a human background K+ channel to sense external pH variations near
physiological pH.
EMBO J. 16 5464-5471 (1997).
 
6. FINK, M., LESAGE, F., DUPRAT, F., HEURTEAUX, C., REYES, R., FOSSET, M.
AND LAZDUNSKI, M.
A neuronal two P domain K+ channel stimulated by arachidonic acid and
polyunsaturated fatty acids.
EMBO J. 17 3297-3308 (1998).

Documentation
Potassium (K+) channels play a key role in many cellular functions, in both
excitable and non-excitable tissues. Among the ion channels, they form the
largest family in terms of both structure and function. K+ channel subunits
contain a conserved pore-forming motif, the P-domain, which is considered
to be an essential element of the aqueous K+-selective pore. Shaker-type
and Kir K+ channel subunits both contain a single P-domain, and four such 
subunits are thought to associate to form a multimer, together with 
associated auxillary (regulatory) subunits. Recently, a new class of K+ 
channel subunit was cloned, which is clearly distinct from the Shaker and
Kir families; the new class contains not one but two P-domains in each
subunit, and evidence suggests a complete channel may be formed by the
dimerisation of two such subunits.
 
The first member of this family (TOK1), cloned from S.cerevisiae [1], is
predicted to have eight potential transmembrane (TM) helices. However,
subsequently-cloned two P-domain family members from Drosophila and
mammalian species are predicted to have only four TM segments. They are
usually referred to as TWIK-related channels (Tandem of P-domains in a 
Weakly Inward rectifying K+ channel) [2-6]. Functional characterisation of
these channels has revealed a diversity of properties in that they may show
inward or outward rectification, their activity may be modulated in 
different directions by protein phosphorylation, and their sensitivity to
changes in intracellular or extracellular pH varies. Despite these
disparate properties, they are all thought to share the same topology of
four TM segments, including two P-domains. That TWIK-related K+ channels
all produce instantaneous and non-inactivating K+ currents, which do not
display a voltage-dependent activation threshold, suggests that they are
background (leak) K+ channels involved in the generation and modulation of
the resting membrane potential in various cell types. Further studies have
revealed that they may be found in many species, including: plants,
invertebrates and mammals.
 
2POREKCHANEL is a 2-element fingerprint that provides a signature for the
two pore domain K+ channel family. The fingerprint was derived from an
initial alignment of 10 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length - motifs 1 and 2 each
encoding part of one of the K+ channel pore-forming domains. Three
iterations on SPTR37_10f were required to reach convergence, at which point
a true set comprising 51 sequences was identified.
 
An update on SPTR57.15_40.15f identified a true set of 345 sequences.
Summary Information
51 codes involving  2 elements
Composite Feature Index
25151
12
True Positives
O00180        O02084        O08581        O14649        
O17185 O17935 O35111 O45422
O45891 O45894 O54912 O62209
O76787 O76788 O76790 O76791
O76792 O76794 O76795 O88454
O95069 O95279 P90863 P97438
Q17481 Q18120 Q18554 Q19525
Q19611 Q19671 Q20817 Q21094
Q21467 Q21505 Q21529 Q21729
Q22042 Q22043 Q22271 Q22426
Q22940 Q23297 Q23386 Q23435
Q93531 Q93764 Q94253 Q94526
Q9Z2T2 TOK1_YEAST TWK8_CAEEL
Sequence Titles
O00180      POTASSIUM CHANNEL KCNO1 - HOMO SAPIENS (HUMAN). 
O02084 SIMILARITY TO S. CEREVISIAE OUTWARD-RECTIFIER POTASSSIUM CHANNEL TOK1 - CAENORHA
O08581 POTASSIUM INWARDLY-RECTIFYING CHANNEL, SUBFAMILY K, MEMBER 1 (TWIK-1 K+ CHANNEL)
O14649 TWIK-RELATED ACID-SENSITIVE K+ CHANNEL - HOMO SAPIENS (HUMAN).
O17185 F34D6.3 PROTEIN - Caenorhabditis elegans.
O17935 K06B4.12 PROTEIN - CAENORHABDITIS ELEGANS.
O35111 CTBAK - MUS MUSCULUS (MOUSE).
O45422 F31D4.7 PROTEIN - CAENORHABDITIS ELEGANS.
O45891 W06D12.2 PROTEIN - CAENORHABDITIS ELEGANS.
O45894 W06D12.5 PROTEIN - CAENORHABDITIS ELEGANS.
O54912 TWIK-RELATED ACID-SENSITIVE K+ CHANNEL - RATTUS NORVEGICUS (RAT).
O62209 F32H5.2 PROTEIN - CAENORHABDITIS ELEGANS.
O76787 PUTATIVE POTASSIUM CHANNEL SUBUNIT N2P17M1-2 - CAENORHABDITIS ELEGANS.
O76788 PUTATIVE POTASSIUM CHANNEL SUBUNIT N2P17M1-1 - CAENORHABDITIS ELEGANS.
O76790 PUTATIVE POTASSIUM CHANNEL SUBUNIT N2P20 - Caenorhabditis elegans.
O76791 PUTATIVE POTASSIUM CHANNEL SUBUNIT N2P17M2-2 - CAENORHABDITIS ELEGANS.
O76792 PUTATIVE POTASSIUM CHANNEL SUBUNIT N2P17M2-3 - CAENORHABDITIS ELEGANS.
O76794 PUTATIVE POTASSIUM CHANNEL SUBUNIT N2P17M2-1 - CAENORHABDITIS ELEGANS.
O76795 PUTATIVE POTASSIUM CHANNEL SUBUNIT N2P38 - Caenorhabditis elegans.
O88454 TWIK-RELATED ARACHIDONIC ACID-STIMULATED POTASSIUM CHANNEL PROTEIN TRAAK - Mus musculus (Mouse).
O95069 OUTWARD RECTIFYING POTASSIUM CHANNEL PROTEIN TREK-1 (TWO-PORE POTASSIUM CHANNEL TPKC1) (TREK-1 K+ CHANNEL SUBUNIT) - Homo sapiens (Human).
O95279 TWO PORE DOMAIN K+ CHANNEL - HOMO SAPIENS (HUMAN).
P90863 F36A2.4 PROTEIN - CAENORHABDITIS ELEGANS.
P97438 OUTWARD RECTIFYING POTASSIUM CHANNEL PROTEIN TREK-1 (TWO-PORE POTASSIUM CHANNEL TPKC1) (TREK-1 K+ CHANNEL SUBUNIT) - Mus musculus (Mouse).
Q17481 B0334.2 PROTEIN - CAENORHABDITIS ELEGANS.
Q18120 SIMILAR TO POTASSIUM CHANNEL PROTEINS AND TO C. ELEGANS PROTEIN F22B7.7 - CAENOR
Q18554 C40C9.1 PROTEIN - CAENORHABDITIS ELEGANS.
Q19525 F17C8.5 PROTEIN - CAENORHABDITIS ELEGANS.
Q19611 SIMILARITY TO PUTATIVE POTASSIUM CHANNEL PROTEINS - CAENORHABDITIS ELEGANS.
Q19671 F21C3.1 PROTEIN - CAENORHABDITIS ELEGANS.
Q20817 F55C5.3 PROTEIN - CAENORHABDITIS ELEGANS.
Q21094 K01D12.4 PROTEIN - CAENORHABDITIS ELEGANS.
Q21467 HYPOTHETICAL 60.4 KD PROTEIN - CAENORHABDITIS ELEGANS.
Q21505 M04B2.5 PROTEIN - CAENORHABDITIS ELEGANS.
Q21529 M110.2 PROTEIN - CAENORHABDITIS ELEGANS.
Q21729 R04F11.4 PROTEIN - CAENORHABDITIS ELEGANS.
Q22042 T01B4.1 PROTEIN - CAENORHABDITIS ELEGANS.
Q22043 T01B4.2 PROTEIN - CAENORHABDITIS ELEGANS.
Q22271 T06H11.1 PROTEIN - CAENORHABDITIS ELEGANS.
Q22426 PROLINE-RICH - CAENORHABDITIS ELEGANS.
Q22940 C52B9.6 PROTEIN - CAENORHABDITIS ELEGANS.
Q23297 ZC410.4 PROTEIN - CAENORHABDITIS ELEGANS.
Q23386 ZK1067.5 PROTEIN - CAENORHABDITIS ELEGANS.
Q23435 ZK1251.8 PROTEIN - CAENORHABDITIS ELEGANS.
Q93531 F19D8.1 PROTEIN - CAENORHABDITIS ELEGANS.
Q93764 F53C11.6 PROTEIN - CAENORHABDITIS ELEGANS.
Q94253 SIMILAR S. CEREVISIAE OUTWARD-RECTIFIER POTASSIUM CHANNEL TOK1 - CAENORHABDITIS
Q94526 TWO P DOMAIN POTASSIUM CHANNEL ORK1 - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q9Z2T2 PUTATIVE POTASSIUM CHANNEL TWIK - Rattus norvegicus (Rat).
TOK1_YEAST OUTWARD-RECTIFIER POTASSIUM CHANNEL TOK1 (TWO-DOMAIN OUTWARD RECTIFIER K+ CHANNE
TWK8_CAEEL TWK-8 PROTEIN - CAENORHABDITIS ELEGANS.
Scan History
SPTR37_10f 3  300  NSINGLE    
Initial Motifs
Motif 1  width=29
Element Seqn Id St Int Rpt
TVITTIGYGHAAPSTDGGKVFCMFYALLG O14649 89 89 -
TVITTIGYGHAAPSTDGGKVFCMFYALLG O54912 89 89 -
TVITTIGFGNISPRTEGGKIFCIIYALLG P97438 138 138 -
TIITTIGYGNIVLHTDAGRLFCIFYALVG O88454 100 100 -
TVCSTVGYGNISPTTFAGRMIMIAYSVIG Q94526 101 101 -
TVLSTTGYGHTVPLSDGGKAFCIIYSVIG Q9Z2T2 113 113 -
TVLSTTGYGHTVPLSDGGKAFCIIYSVIG O08581 113 113 -
TCITTIGYGELVCHTIAGKLVTVAYGIIG O02084 158 158 -
TVVTTIGYGNPVPVTNIGRIWCILFSLLG TWK8_CAEEL 39 39 -
TTIIPVGYGYIAPLTSTGRIVLCIYAAFG Q94253 149 149 -

Motif 2 width=10
Element Seqn Id St Int Rpt
TTIGFGDYVA O14649 198 80 -
TTIGFGDYVA O54912 198 80 -
TTIGFGDYVA P97438 250 83 -
TTVGFGDYVP O88454 212 83 -
TTIGFGDYVP Q94526 216 86 -
STIGLGDYVP Q9Z2T2 224 82 -
STIGLGDYVP O08581 224 82 -
STIGFGDFVP O02084 268 81 -
TTVGFGDLMP TWK8_CAEEL 172 104 -
FTIGFGDITP Q94253 261 83 -
Final Motifs
Motif 1  width=29
Element Seqn Id St Int Rpt
TAITTIGYGHIVPRTDEGRVAIIFYALLG Q21729 125 125 -
TVITTIGYGHAAPSTDGGKVFCMFYALLG O14649 89 89 -
TVITTIGYGHAAPSTDGGKVFCMFYALLG O35111 89 89 -
TVITTIGYGHAAPSTDGGKVFCMFYALLG O54912 89 89 -
TVITTIGYGHVAPETFEGRLFLIFYGVIG Q23435 172 172 -
TVITTIGYGHSTPMTDAGKVFCMLYALAG O17185 96 96 -
TVITTIGYGHSTPMTDAGKVFCMLYALAG O76795 89 89 -
TVITTIGYGNVAPQSFWGRVFVIIYGTIG O62209 142 142 -
TVITTIGFGNISPRTEGGKIFCIIYALLG O95069 153 153 -
TVITTIGFGNISPRTEGGKIFCIIYALLG P97438 138 138 -
TVITTIGYGNVAPKTPAGRLFCVFYGLFG O95279 94 94 -
TIITTIGYGNIVLHTDAGRLFCIFYALVG O88454 100 100 -
TIFTTIGYGNLACKTNLGRIATIIYGMIG Q22042 136 136 -
VVITTVGYGHSAPSTNAGKLFCMIFALFG O76790 92 92 -
VVITTVGYGHSAPSTNAGKLFCMIFALFG Q18554 92 92 -
TVYTTIGYGNIVPGTGWGRFATILYAFIG Q18120 125 125 -
TTMATIGYGNIVPVTPLGRLACVLFALFG Q22426 339 339 -
TVCSTVGYGNISPTTFAGRMIMIAYSVIG Q94526 101 101 -
TVLSTTGYGHTVPLSDGGKAFCIIYSVIG O00180 113 113 -
TVLSTTGYGHTVPLSDGGKAFCIIYSVIG Q9Z2T2 113 113 -
TVLSTTGYGHTVPLSDGGKAFCIIYSVIG O08581 113 113 -
TILTTIGYGNVTPHTQQCKVFLMIYGAFG Q93531 151 151 -
TCITTIGYGELVCHTIAGKLVTVAYGIIG O02084 158 158 -
TICTTIGYGHIYPMTDAGRMLTMIFALFG O45422 121 121 -
TIYTTIGYGDLAAETIWGRICTMLYAMIG O45891 121 121 -
TIFTTIGYGNIACKTRAGQIATMVYAFVG Q19611 184 184 -
TVVTTIGYGNPVPVTNIGRIWCILFSLLG TWK8_CAEEL 39 39 -
TTISTIGYGNIAPFDDRGKVICILYCVAG Q19671 111 111 -
TMLTSIGYGYVAPSTFGGRLFGVIYCLIG P90863 167 167 -
TLFSTIGYGTISCQTVWGRTLSMIYASIG Q21467 196 196 -
SLYTTIGYGNFHPLTKSGRIISMMYACIG Q20817 233 233 -
TVISTIGFGTSTPRTHLGRFITIVYGVVG Q21094 132 132 -
TTIIPVGYGYIAPLTSTGRIVLCIYAAFG Q94253 149 149 -
NVYTTTGYGSIAPSSSLGKALVIVYGLIF Q21505 254 254 -
RVVFSRGYGNLVPITVTGRVACIIFALLG Q22940 167 167 -
TVITSTGYDHVTPATDPGRIFTVFFGLIG O76787 190 190 -
TVITSTGYDHVTPATDPGRIFTVFFGLIG O76794 254 254 -
TVITSTGYDHVTPATDPGRIFTVFFGLIG O76792 254 254 -
TVITSTGYDHVTPATDPGRIFTVFFGLIG O76791 254 254 -
TVITSTGYDHVTPATDPGRIFTVFFGLIG O76788 190 190 -
NVYTTTGYGSIAPESILGQVLVCLYGFIF O17935 241 241 -
TLFTTIGYGDIACITSAGRIATVIYSCVG Q23297 188 188 -
NVYTTTGYGSIAPESTLGQVLVCVYGFIF O45894 325 325 -
TVLTSIGYGNLIPISTGGKIFCVGYAIFG Q23386 233 233 -
SILIFSGYGIDAPESLIGRVFCLVYLFFG Q93764 129 129 -
TTVIPVGYGYIFPVSAYGRMCLIAYALLG Q21529 145 145 -
TVLTTCGYGEVSVDTDVGKVFSVAFALVG Q22271 299 299 -
ITISTIGYGNQYPQTHASRVFSIFFSILG Q17481 65 65 -
IRGEGWRYGNLACKTNLGRVATIIYGLIG Q22043 260 260 -
TLYSTVGYGSLYPHSTLGRYLTIFYSLLM Q19525 118 118 -
VSLLTVGLGDILPKSVGAKIMVLIFSLSG TOK1_YEAST 283 283 -

Motif 2 width=10
Element Seqn Id St Int Rpt
TTIGFGDIVP Q21729 335 181 -
TTIGFGDYVA O14649 198 80 -
TTIGFGDYVA O35111 198 80 -
TTIGFGDYVA O54912 198 80 -
TTIGLGDFVP Q23435 355 154 -
TTIGFGDYVA O17185 204 79 -
TTIGFGDYVA O76795 197 79 -
TTIGLGDLVP O62209 297 126 -
TTIGFGDYVA O95069 265 83 -
TTIGFGDYVA P97438 250 83 -
STIGFGDFVA O95279 202 79 -
TTVGFGDYVP O88454 212 83 -
STIGFGDIVP Q22042 273 108 -
STIGFGDLVP O76790 205 84 -
STIGFGDLVP Q18554 205 84 -
TTIGFGDILP Q18120 265 111 -
TTIGFGDIVP Q22426 482 114 -
TTIGFGDYVP Q94526 216 86 -
STIGLGDYVP O00180 224 82 -
STIGLGDYVP Q9Z2T2 224 82 -
STIGLGDYVP O08581 224 82 -
TTIGLGDIVP Q93531 270 90 -
STIGFGDFVP O02084 268 81 -
STVGLGDLVP O45422 251 101 -
TTIGFGDFTP O45891 264 114 -
TTIGLGDVTP Q19611 353 140 -
TTVGFGDLMP TWK8_CAEEL 172 104 -
TTIGYGDYSP Q19671 202 62 -
LTVGFGDIRP P90863 303 107 -
TTIGLGDVMP Q21467 318 93 -
TTIGLGDVVT Q20817 402 140 -
ATIGFGDYVS Q21094 272 111 -
FTIGFGDITP Q94253 261 83 -
STIGLGDVMP Q21505 378 95 -
ATVGFGDIVP Q22940 318 122 -
TTIGLGNIRV O76787 343 124 -
TTIGLGNIRV O76794 407 124 -
TTIGLGNIRV O76792 407 124 -
TTIGLGNIRV O76791 407 124 -
TTIGLGNIRV O76788 343 124 -
STIGLGDIMP O17935 365 95 -
STIGLGDVSV Q23297 355 138 -
STIGLGDIMP O45894 449 95 -
LTVGFGDLHP Q23386 332 70 -
LTIGFGDLVP Q93764 277 119 -
FTIGFGDLTP Q21529 243 69 -
VTVGLGDIVV Q22271 454 126 -
ATVGFGDKVP Q17481 174 80 -
STIGFGDVIP Q22043 398 109 -
FLIGIGDIVP Q19525 242 95 -
LTIGYGDYAP TOK1_YEAST 420 108 -