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PR01332

Identifier
KIR62CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01332
No. of Motifs
7
Creation Date
11-MAY-2000
Title
Kir6.2 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
PRODOM; PD015313; PD017574
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).
 
4. ASHCROFT, F.M. AND GRIBBLE, F.M.
Correlating structure and function in ATP-sensitive K+ channels.
TRENDS NEUROSCIENCE 21 288-294 (1998).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
Kir6.x inward rectifier K+ channel family subunits associate with
sulphonylurea receptors (SURs) to form ATP-sensitive K+ (KATP) channels.
These are octameric complexes containing equal numbers of Kir and SUR
subunits. The resulting channels are regulated by cytosolic nucleotides,
such as ATP, and thus link cell metabolism to electrical activity, and K+
fluxes. They are important in the regulation of insulin secretion, the
control of vascular and smooth muscle tone, and the response of the heart
(and possibly the brain) to ischaemia.
 
Kir6.2 (also known as BIR/KATP) is thought to associate with SUR1 to form
the pancreatic beta cell KATP channel that is involved in regulation of
insulin secretion. Combination of Kir6.2 with splice variants of SUR2 is
thought to produce the cardiac and smooth muscle KATP isoforms [4].
 
KIR62CHANNEL is a 7-element fingerprint that provides a signature for the
Kir6.2 inward rectifier K+ channel. The fingerprint was derived from an
initial alignment of 4 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise Kir6.2 but distinguish it from other subtypes -
motif 1 resides within the putative cytoplasmic C-terminus; motifs 2-3 lie
between the first TM domain and the following pore-forming (H5) domain; and
motifs 4-7 encode portions of the cytoplasmic C-terminus. A single
iteration on SPTR37_10f was required to reach convergence, no further
sequences being identified beyond the starting set.
Summary Information
4 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
74444444
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
IRKB_HUMAN    IRKB_MOUSE    IRKB_RAT      O02822        
Sequence Titles
IRKB_HUMAN  ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 11 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 11) (INWARD RECTIFIER POTASSIUM CHANNEL KIR6.2) (IKATP) - HOMO SAPIENS (HUMAN). 
IRKB_MOUSE ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 11 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 11) (INWARD RECTIFIER POTASSIUM CHANNEL KIR6.2) - MUS MUSCULUS (MOUSE).
IRKB_RAT ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 11 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 11) (INWARD RECTIFIER POTASSIUM CHANNEL KIR6.2) (ATP-SENSITIVE INWARDLY RECTIFYING K+ CHANNEL, BIR) - RATTUS NORVEGICUS (RAT).
O02822 ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL KIR6.2 - ORYCTOLAGUS CUNICULUS (RABBIT).
Scan History
SPTR37_10f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
DPAEPRYRTRER IRKB_MOUSE 20 20 -
DPTEPRYRTRER IRKB_RAT 20 20 -
DPAEPRYRARER O02822 20 20 -
DPAEPRYRARQR IRKB_HUMAN 20 20 -

Motif 2 width=10
Element Seqn Id St Int Rpt
LAPGEGTNVP IRKB_MOUSE 100 68 -
LAPGEGTNVP IRKB_RAT 100 68 -
LAPGEGAAVP O02822 100 68 -
LAPSEGTAEP IRKB_HUMAN 100 68 -

Motif 3 width=8
Element Seqn Id St Int Rpt
SIHSFSSA IRKB_MOUSE 113 3 -
SIHSFSSA IRKB_RAT 113 3 -
SIHSFSSA O02822 113 3 -
SIHSFSSA IRKB_HUMAN 113 3 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LHQVDIPMENGVG IRKB_MOUSE 233 112 -
LHQVDIPMENGVG IRKB_RAT 233 112 -
LHQVDIPMENGVG O02822 233 112 -
LHQVDIPMENGVG IRKB_HUMAN 233 112 -

Motif 5 width=11
Element Seqn Id St Int Rpt
YDLAPSDLHHH IRKB_MOUSE 268 22 -
YDLAPSDLHHH IRKB_RAT 268 22 -
YDLAPSDLHHH O02822 268 22 -
YDLAPSDLHHH IRKB_HUMAN 268 22 -

Motif 6 width=10
Element Seqn Id St Int Rpt
DRSLLDALTL IRKB_MOUSE 352 73 -
DRSLLDALTL IRKB_RAT 352 73 -
DRSLLDALTL O02822 352 73 -
DHSLLEALTL IRKB_HUMAN 352 73 -

Motif 7 width=19
Element Seqn Id St Int Rpt
SVAVAKAKPKFSISPDSLS IRKB_MOUSE 372 10 -
SVAVAKAKPKFSISPDSLS IRKB_RAT 372 10 -
SVPVAKAKPKFSISPDSLS O02822 372 10 -
SVPMAKAKPKFSISPDSLS IRKB_HUMAN 372 10 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
DPAEPRYRTRER IRKB_MOUSE 20 20 -
DPTEPRYRTRER IRKB_RAT 20 20 -
DPAEPRYRARER O02822 20 20 -
DPAEPRYRARQR IRKB_HUMAN 20 20 -

Motif 2 width=10
Element Seqn Id St Int Rpt
LAPGEGTNVP IRKB_MOUSE 100 68 -
LAPGEGTNVP IRKB_RAT 100 68 -
LAPGEGAAVP O02822 100 68 -
LAPSEGTAEP IRKB_HUMAN 100 68 -

Motif 3 width=8
Element Seqn Id St Int Rpt
SIHSFSSA IRKB_MOUSE 113 3 -
SIHSFSSA IRKB_RAT 113 3 -
SIHSFSSA O02822 113 3 -
SIHSFSSA IRKB_HUMAN 113 3 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LHQVDIPMENGVG IRKB_MOUSE 233 112 -
LHQVDIPMENGVG IRKB_RAT 233 112 -
LHQVDIPMENGVG O02822 233 112 -
LHQVDIPMENGVG IRKB_HUMAN 233 112 -

Motif 5 width=11
Element Seqn Id St Int Rpt
YDLAPSDLHHH IRKB_MOUSE 268 22 -
YDLAPSDLHHH IRKB_RAT 268 22 -
YDLAPSDLHHH O02822 268 22 -
YDLAPSDLHHH IRKB_HUMAN 268 22 -

Motif 6 width=10
Element Seqn Id St Int Rpt
DRSLLDALTL IRKB_MOUSE 352 73 -
DRSLLDALTL IRKB_RAT 352 73 -
DRSLLDALTL O02822 352 73 -
DHSLLEALTL IRKB_HUMAN 352 73 -

Motif 7 width=19
Element Seqn Id St Int Rpt
SVAVAKAKPKFSISPDSLS IRKB_MOUSE 372 10 -
SVAVAKAKPKFSISPDSLS IRKB_RAT 372 10 -
SVPVAKAKPKFSISPDSLS O02822 372 10 -
SVPMAKAKPKFSISPDSLS IRKB_HUMAN 372 10 -