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PR01328

Identifier
KIR32CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01328
No. of Motifs
6
Creation Date
11-MAY-2000
Title
Kir3.2 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
PRODOM; PD012467; PD017575
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).
 
4. INANOBE, A., YOSHIMOTO, Y., HORIO, Y., MORISHIGE, K.I., HIBINO, H.,
MATSUMOTO, S., TOKUNAGA, Y., MAEDA, T., HATA, Y., TAKAI, Y. AND KURACHI, Y.
Characterization of G protein-gated K+ channels composed of Kir3.2 subunits
in dopaminergic neurons of the substantia nigra. 
J.NEUROSCIENCE 19 1006-1017 (1999).
 
5. ABRAHAM, M.R., JAHANGIR, A., ALEKSEEV, A.E. AND TERZIC, A.
Channelopathies of inwardly rectifying potassium channnels.
FASEB J. 13 1901-1910 (1999).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
The Kir3.x channel family are gated by G proteins following G-protein
coupled receptor (GPCR) activation. They are widely distributed in
neuronal, atrial, and endocrine tissues and play key roles in generating
late inhibitory postsynaptic potentials, slowing the heart rate and
modulating hormone release. They are directly activated by G protein
beta-gamma subunits released from G protein heterotrimers of the G(i/o)
family upon appropriate receptor stimulation.
 
Kir3.2 is thought to associate with Kir3.1 to form Kir channel heteromers
in heart tissue. In central neurones, Kir3.2 homomers may exist, although
these may contain combinations of the three splice variants of Kir3.2 that
have been identified [4]. Weaver mice, which suffer neurological and
reproductive deficits, have a point mutation in the gene encoding Kir3.2.
This lies in the pore-forming domain of the channel, and as a result they
lose their selectivity for K+, allowing Na+ to pass through the channel 
pore [5].
 
KIR32CHANNEL is a 6-element fingerprint that provides a signature for the
Kir3.2 inward rectifier K+ channel. The fingerprint was derived from an
initial alignment of 4 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise Kir3.2 but distinguish it from other subtypes -
motifs 1-2 lie within the putative cytoplasmic N-terminus; and motifs 3-6
encode portions of the cytoplasmic C-terminus. Two iterations on SPTR37_10f
were required to reach convergence, at which point a true set comprising 5
sequences was identified.
Summary Information
5 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6555555
5000000
4000000
3000000
2000000
123456
True Positives
IRK6_HUMAN    IRK6_MESAU    IRK6_MOUSE    IRK6_RAT      
O70290
Sequence Titles
IRK6_HUMAN  G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 2 (GIRK2) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 6) (KATP-2) (BIR1) (KIR3.2) - HOMO SAPIENS (HUMAN). 
IRK6_MESAU G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 2 (GIRK2) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 6) (KATP-2) (KIR3.2) - MESOCRICETUS AURATUS (GOLDEN HAMSTER).
IRK6_MOUSE G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 2 (GIRK2) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 6) (KIR3.2) - MUS MUSCULUS (MOUSE).
IRK6_RAT G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 2 (GIRK2) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 6) (BIR1) (KATP-2) (KIR3.2) - RATTUS NORVEGICUS (RAT).
O70290 G-PROTEIN-COUPLED INWARD RECTIFIER K+ CHANNEL 2-A - MUS MUSCULUS (MOUSE).
Scan History
SPTR37_10f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
MAKLTESMTNVLEGD IRK6_RAT 3 3 -
MAKLTESMTNVLEGD IRK6_MESAU 3 3 -
MAKLTESMTNVLEGD IRK6_MOUSE 3 3 -
MAKLTESMTNVLEGD IRK6_HUMAN 1 1 -

Motif 2 width=21
Element Seqn Id St Int Rpt
HQPKLPKQARDDLPRHISRDR IRK6_RAT 30 12 -
HQPKLPKQARDDLPRHISRDR IRK6_MESAU 30 12 -
HQPKLPKQARDDLPRHISRDR IRK6_MOUSE 30 12 -
HQPKLPKQARDDLPRHISRDR IRK6_HUMAN 28 12 -

Motif 3 width=9
Element Seqn Id St Int Rpt
ISKAQLPKE IRK6_RAT 294 243 -
ISKAQLPKE IRK6_MESAU 294 243 -
ISKAQLPKE IRK6_MOUSE 294 243 -
ISKAQLPKE IRK6_HUMAN 292 243 -

Motif 4 width=14
Element Seqn Id St Int Rpt
LANRAELPLSWSVS IRK6_RAT 375 72 -
LANRAELPLSWSVS IRK6_MESAU 375 72 -
LANRAEVPLSWSVS IRK6_MOUSE 375 72 -
LASRAELPLSWSVS IRK6_HUMAN 373 72 -

Motif 5 width=10
Element Seqn Id St Int Rpt
KLNQHAELET IRK6_RAT 390 1 -
KLNQHAELET IRK6_MESAU 390 1 -
KLNQHAELET IRK6_MOUSE 390 1 -
KLNQHAELET IRK6_HUMAN 388 1 -

Motif 6 width=18
Element Seqn Id St Int Rpt
EEKNPEELTERNGDVANL IRK6_RAT 402 2 -
EEKNPEEQTERNGDVANL IRK6_MESAU 402 2 -
EEKNPEELTERNGDVANL IRK6_MOUSE 402 2 -
EEKNLEEQTERNGDVANL IRK6_HUMAN 400 2 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
MAKLTESMTNVLEGD IRK6_RAT 3 3 -
MAKLTESMTNVLEGD IRK6_MESAU 3 3 -
MAKLTESMTNVLEGD IRK6_MOUSE 3 3 -
MAKLTESMTNVLEGD O70290 3 3 -
MAKLTESMTNVLEGD IRK6_HUMAN 1 1 -

Motif 2 width=21
Element Seqn Id St Int Rpt
HQPKLPKQARDDLPRHISRDR IRK6_RAT 30 12 -
HQPKLPKQARDDLPRHISRDR IRK6_MESAU 30 12 -
HQPKLPKQARDDLPRHISRDR IRK6_MOUSE 30 12 -
HQPKLPKQARDDLPRHISRDR O70290 30 12 -
HQPKLPKQARDDLPRHISRDR IRK6_HUMAN 28 12 -

Motif 3 width=9
Element Seqn Id St Int Rpt
ISKAQLPKE IRK6_RAT 294 243 -
ISKAQLPKE IRK6_MESAU 294 243 -
ISKAQLPKE IRK6_MOUSE 294 243 -
ISKAQLPKE O70290 294 243 -
ISKAQLPKE IRK6_HUMAN 292 243 -

Motif 4 width=14
Element Seqn Id St Int Rpt
LANRAELPLSWSVS IRK6_RAT 375 72 -
LANRAELPLSWSVS IRK6_MESAU 375 72 -
LANRAEVPLSWSVS IRK6_MOUSE 375 72 -
LANRAEVPLSWSVS O70290 375 72 -
LASRAELPLSWSVS IRK6_HUMAN 373 72 -

Motif 5 width=10
Element Seqn Id St Int Rpt
KLNQHAELET IRK6_RAT 390 1 -
KLNQHAELET IRK6_MESAU 390 1 -
KLNQHAELET IRK6_MOUSE 390 1 -
KLNQHAELET O70290 390 1 -
KLNQHAELET IRK6_HUMAN 388 1 -

Motif 6 width=18
Element Seqn Id St Int Rpt
EEKNPEELTERNGDVANL IRK6_RAT 402 2 -
EEKNPEEQTERNGDVANL IRK6_MESAU 402 2 -
EEKNPEELTERNGDVANL IRK6_MOUSE 402 2 -
EEKNPEELTERNGDVANL O70290 402 2 -
EEKNLEEQTERNGDVANL IRK6_HUMAN 400 2 -