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PR01327

Identifier
KIR31CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01327
No. of Motifs
12
Creation Date
11-MAY-2000
Title
Kir3.1 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
PRODOM; PD024038; PD029156
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
The Kir3.x channel family are gated by G proteins following G-protein
coupled receptor (GPCR) activation. They are widely distributed in
neuronal, atrial, and endocrine tissues and play key roles in generating
late inhibitory postsynaptic potentials, slowing the heart rate and
modulating hormone release. They are directly activated by G protein beta-
gamma subunits released from G protein heterotrimers of the G(i/o) family
upon appropriate receptor stimulation.
 
Kir3.1 channels are thought to form heteromers in vivo: in heart,
consisting of Kir3.1 and Kir3.2, and in brain, Kir3.1 with Kir 3.4. 
 
KIR31CHANNEL is a 12-element fingerprint that provides a signature for the
Kir3.1 inward rectifier K+ channel. The fingerprint was derived from an
an initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise Kir3.1 but distinguish it from other subtypes -
motif 1 encodes the first 24 residues of the putative cytoplasmic
N-terminus; motif 2 lies between the first TM domain and the following pore
-forming (H1) domain; and motifs 3-12 encode portions of the cytoplasmic
C-terminus. A single iteration on SPTR37_10f was required to reach
convergence, no further sequences beiing identified beyond the starting
set.
Summary Information
3 codes involving 12 elements
0 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
12333333333333
11000000000000
10000000000000
9000000000000
8000000000000
7000000000000
6000000000000
5000000000000
4000000000000
3000000000000
2000000000000
123456789101112
True Positives
IRK3_CHICK    IRK3_HUMAN    IRK3_MOUSE    
Sequence Titles
IRK3_CHICK  G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 1 (GIRK1) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 3) - GALLUS GALLUS (CHICKEN). 
IRK3_HUMAN G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 1 (GIRK1) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 3) (KIR3.1) - HOMO SAPIENS (HUMAN).
IRK3_MOUSE G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 1 (GIRK1) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 3) (KGA) (KGB1) (KIR3.1) - MUS MUSCULUS (MOUSE), AND RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
MSALRRKFGDDYQVVTTSSSGSGL IRK3_HUMAN 1 1 -
MSALRRKFGDDYQVVTTSSSGSGL IRK3_MOUSE 1 1 -
MSALRRKLGDEYQVVSTSASGGGL IRK3_CHICK 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
NYTPCVANVYNFPS IRK3_HUMAN 119 94 -
NYTPCVANVYNFPS IRK3_MOUSE 119 94 -
SYTPCVANVYNFPS IRK3_CHICK 111 86 -

Motif 3 width=14
Element Seqn Id St Int Rpt
QLELDVGFSTGADQ IRK3_HUMAN 248 115 -
QLELDVGFSTGADQ IRK3_MOUSE 248 115 -
QLELDVGFSTGADQ IRK3_CHICK 240 115 -

Motif 4 width=13
Element Seqn Id St Int Rpt
FYDLSQRSMQTEQ IRK3_HUMAN 280 18 -
FYDLSQRSMQTEQ IRK3_MOUSE 280 18 -
FYDLSQRTMQTEQ IRK3_CHICK 272 18 -

Motif 5 width=16
Element Seqn Id St Int Rpt
PLIAPAITNSKERHNS IRK3_HUMAN 370 77 -
PLIAPAITNSKERHNS IRK3_MOUSE 370 77 -
PLIAPAVSNSKERNNS IRK3_CHICK 362 77 -

Motif 6 width=14
Element Seqn Id St Int Rpt
VECLDGLDDITTKL IRK3_HUMAN 386 0 -
VECLDGLDDISTKL IRK3_MOUSE 386 0 -
VECLDGLDEVGIKL IRK3_CHICK 378 0 -

Motif 7 width=14
Element Seqn Id St Int Rpt
PSKLQKITGREDFP IRK3_HUMAN 400 0 -
PSKLQKITGREDFP IRK3_MOUSE 400 0 -
PSKLQKITGRDDFP IRK3_CHICK 392 0 -

Motif 8 width=13
Element Seqn Id St Int Rpt
KKLLRMSSTTSEK IRK3_HUMAN 414 0 -
KKLLRMSSTTSEK IRK3_MOUSE 414 0 -
KKLLRISSTTSEK IRK3_CHICK 406 0 -

Motif 9 width=16
Element Seqn Id St Int Rpt
AYSLGDLPMKLQRISS IRK3_HUMAN 427 0 -
AYSLGDLPMKLQRISS IRK3_MOUSE 427 0 -
AYSMGDLPMKLQRISS IRK3_CHICK 419 0 -

Motif 10 width=15
Element Seqn Id St Int Rpt
VPGNSEEKLVSKTTK IRK3_HUMAN 443 0 -
VPGNSEEKLVSKTTK IRK3_MOUSE 443 0 -
VPGNSEEKLVSKATK IRK3_CHICK 435 0 -

Motif 11 width=16
Element Seqn Id St Int Rpt
LSDPMSQSVADLPPKL IRK3_HUMAN 459 1 -
LSDPMSQSVADLPPKL IRK3_MOUSE 459 1 -
MSDPMSQSVADLPPKL IRK3_CHICK 451 1 -

Motif 12 width=19
Element Seqn Id St Int Rpt
RMEGNLPAKLRKMNSDRFT IRK3_HUMAN 483 8 -
RMEGNLPAKLRKMNSDRFT IRK3_MOUSE 483 8 -
RMEGNLPPKLRKMNSDRFT IRK3_CHICK 474 7 -
Final Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
MSALRRKFGDDYQVVTTSSSGSGL IRK3_HUMAN 1 1 -
MSALRRKFGDDYQVVTTSSSGSGL IRK3_MOUSE 1 1 -
MSALRRKLGDEYQVVSTSASGGGL IRK3_CHICK 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
NYTPCVANVYNFPS IRK3_HUMAN 119 94 -
NYTPCVANVYNFPS IRK3_MOUSE 119 94 -
SYTPCVANVYNFPS IRK3_CHICK 111 86 -

Motif 3 width=14
Element Seqn Id St Int Rpt
QLELDVGFSTGADQ IRK3_HUMAN 248 115 -
QLELDVGFSTGADQ IRK3_MOUSE 248 115 -
QLELDVGFSTGADQ IRK3_CHICK 240 115 -

Motif 4 width=13
Element Seqn Id St Int Rpt
FYDLSQRSMQTEQ IRK3_HUMAN 280 18 -
FYDLSQRSMQTEQ IRK3_MOUSE 280 18 -
FYDLSQRTMQTEQ IRK3_CHICK 272 18 -

Motif 5 width=16
Element Seqn Id St Int Rpt
PLIAPAITNSKERHNS IRK3_HUMAN 370 77 -
PLIAPAITNSKERHNS IRK3_MOUSE 370 77 -
PLIAPAVSNSKERNNS IRK3_CHICK 362 77 -

Motif 6 width=14
Element Seqn Id St Int Rpt
VECLDGLDDITTKL IRK3_HUMAN 386 0 -
VECLDGLDDISTKL IRK3_MOUSE 386 0 -
VECLDGLDEVGIKL IRK3_CHICK 378 0 -

Motif 7 width=14
Element Seqn Id St Int Rpt
PSKLQKITGREDFP IRK3_HUMAN 400 0 -
PSKLQKITGREDFP IRK3_MOUSE 400 0 -
PSKLQKITGRDDFP IRK3_CHICK 392 0 -

Motif 8 width=13
Element Seqn Id St Int Rpt
KKLLRMSSTTSEK IRK3_HUMAN 414 0 -
KKLLRMSSTTSEK IRK3_MOUSE 414 0 -
KKLLRISSTTSEK IRK3_CHICK 406 0 -

Motif 9 width=16
Element Seqn Id St Int Rpt
AYSLGDLPMKLQRISS IRK3_HUMAN 427 0 -
AYSLGDLPMKLQRISS IRK3_MOUSE 427 0 -
AYSMGDLPMKLQRISS IRK3_CHICK 419 0 -

Motif 10 width=15
Element Seqn Id St Int Rpt
VPGNSEEKLVSKTTK IRK3_HUMAN 443 0 -
VPGNSEEKLVSKTTK IRK3_MOUSE 443 0 -
VPGNSEEKLVSKATK IRK3_CHICK 435 0 -

Motif 11 width=16
Element Seqn Id St Int Rpt
LSDPMSQSVADLPPKL IRK3_HUMAN 459 1 -
LSDPMSQSVADLPPKL IRK3_MOUSE 459 1 -
MSDPMSQSVADLPPKL IRK3_CHICK 451 1 -

Motif 12 width=19
Element Seqn Id St Int Rpt
RMEGNLPAKLRKMNSDRFT IRK3_HUMAN 483 8 -
RMEGNLPAKLRKMNSDRFT IRK3_MOUSE 483 8 -
RMEGNLPPKLRKMNSDRFT IRK3_CHICK 474 7 -