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PR01325

Identifier
KIR22CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01325
No. of Motifs
12
Creation Date
12-MAY-2000
Title
Kir2.2 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).
 
4. NAMBA, N., INAGAKI, N., GONOI, T., SEINO, Y. AND SEINO, S.
Kir2.2v: a possible negative regulator of the inwardly rectifying K+
channel Kir2.2. 
FEBS LETT. 386 211-214 (1996).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
Kir2.2 (also known as IRK2/BIR8), like other Kir2.x family members, has
been found to be expressed in the brain. Immuno-localisation studies have
revealed it is primarily expressed in the cerebellum, as opposed to the
forebrain (cf. Kir1.1). It is also expressed to lower extents in the
kidney, heart and skeletal muscle. When heterologously expressed in Xenopus
oocytes, human Kir2.2 produced strong, inwardly rectifying K+ currents.
Co-expression of Kir2.2v (a closely related Kir2.x subunit) with Kir2.2
caused an inhibition of induced K+ currents, indicating that it likely
functions as a negative regulator of Kir2.2 [4].
 
KIR22CHANNEL is a 12-element fingerprint that provides a signature for the
Kir2.2 inward rectifier K+ channel. The fingerprint was derived from an
initial alignment of 4 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise Kir2.2 but distinguish it from other subtypes -
motifs 1-2 lie in the putative cytoplasmic N-terminus; motifs 3-4 lie
between the first TM domain and the following pore-forming (H5) domain; and
motifs 5-12 encode portions of the cytoplasmic C-terminus. A single
iteration on SPTR37_10f was required to reach convergence, no further
sequences being identified beyond the starting set. One partial match was
also identified, O43401, a closely related human sequence, identified as an
inwardly rectifying K+ channel negative regulator, which lacks the
N-terminal third of the molecule bearing the first 4 motifs.
Summary Information
   4 codes involving 12 elements
0 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
1 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
12444444444444
11000000000000
10000000000000
9000000000000
8000011111111
7000000000000
6000000000000
5000000000000
4000000000000
3000000000000
2000000000000
123456789101112
True Positives
IRKC_HUMAN    IRKC_MOUSE    IRKC_RAT      IRKI_HUMAN    
True Positive Partials
Codes involving 8 elements
O43401
Sequence Titles
IRKC_HUMAN  ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 12 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 12) (INWARD RECTIFIER POTASSIUM CHANNEL IRK2) (KIR2.2) - HOMO SAPIENS (HUMAN). 
IRKC_MOUSE ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 12 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 12) (INWARD RECTIFIER POTASSIUM CHANNEL IRK2) (KIR2.2) - MUS MUSCULUS (MOUSE).
IRKC_RAT ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 12 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 12) (INWARD RECTIFIER POTASSIUM CHANNEL IRK2) (KIR2.2) - RATTUS NORVEGICUS (RAT).
IRKI_HUMAN INWARD RECTIFYING K+ CHANNEL NEGATIVE REGULATOR KIR2.2V - HOMO SAPIENS (HUMAN).

O43401 INWARD RECTIFYING K+ CHANNEL NEGATIVE REGULATOR - HOMO SAPIENS (HUMAN).
Scan History
SPTR37_10f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
MTAASRANP IRKC_HUMAN 1 1 -
MTAASRANP IRKC_RAT 1 1 -
MTAASRANP IRKI_HUMAN 1 1 -
MTAASRANP IRKC_MOUSE 1 1 -

Motif 2 width=8
Element Seqn Id St Int Rpt
IEFANMDE IRKC_HUMAN 55 45 -
IEFANMDE IRKC_RAT 55 45 -
IEFANMDE IRKI_HUMAN 55 45 -
IEFANMDE IRKC_MOUSE 55 45 -

Motif 3 width=9
Element Seqn Id St Int Rpt
PAEGRGRTP IRKC_HUMAN 114 51 -
PAEGRGRTP IRKC_RAT 114 51 -
PAEGRGRTP IRKI_HUMAN 114 51 -
PAEGRGRTP IRKC_MOUSE 114 51 -

Motif 4 width=9
Element Seqn Id St Int Rpt
VMQVHGFMA IRKC_HUMAN 124 1 -
VLQVHGFMA IRKC_RAT 124 1 -
VMQVHGFMA IRKI_HUMAN 124 1 -
VLQVHGFMA IRKC_MOUSE 124 1 -

Motif 5 width=9
Element Seqn Id St Int Rpt
IKPRVTEEG IRKC_HUMAN 233 100 -
IKPRVTEEG IRKC_RAT 233 100 -
IKPRVTKEG IRKI_HUMAN 233 100 -
IKPRVTEEG IRKC_MOUSE 233 100 -

Motif 6 width=8
Element Seqn Id St Int Rpt
QDLETDDF IRKC_HUMAN 285 43 -
QDLETDDF IRKC_RAT 286 44 -
QDLQMDDF IRKI_HUMAN 286 44 -
QDLETDDF IRKC_MOUSE 286 44 -

Motif 7 width=7
Element Seqn Id St Int Rpt
NQYKIDY IRKC_HUMAN 335 42 -
NQYKIDY IRKC_RAT 336 42 -
NQYKIDY IRKI_HUMAN 335 41 -
NQYKIDY IRKC_MOUSE 336 42 -

Motif 8 width=9
Element Seqn Id St Int Rpt
VENKFLLPS IRKC_HUMAN 362 20 -
VENKFLLPS IRKC_RAT 363 20 -
VENKFLLPR IRKI_HUMAN 362 20 -
VENKFLLPS IRKC_MOUSE 363 20 -

Motif 9 width=8
Element Seqn Id St Int Rpt
LAFLSRDE IRKC_HUMAN 380 9 -
LAFLSRDE IRKC_RAT 381 9 -
LAFLSRDE IRKI_HUMAN 380 9 -
LAFLIRDE IRKC_MOUSE 381 9 -

Motif 10 width=12
Element Seqn Id St Int Rpt
EDEADGDQDGRS IRKC_HUMAN 388 0 -
EDEVATDRDGRS IRKC_RAT 389 0 -
EDEADGDQDGRS IRKI_HUMAN 388 0 -
EDEVSTDRDVRT IRKC_MOUSE 389 0 -

Motif 11 width=10
Element Seqn Id St Int Rpt
PQARHDFDRL IRKC_HUMAN 405 5 -
PQPEHDFDRL IRKC_RAT 401 0 -
PQARHDFDRL IRKI_HUMAN 405 5 -
PQPEHDFDRL IRKC_MOUSE 401 0 -

Motif 12 width=8
Element Seqn Id St Int Rpt
QAGGGVLE IRKC_HUMAN 415 0 -
QASSGALE IRKC_RAT 411 0 -
QAGGGVLE IRKI_HUMAN 415 0 -
QASSAALV IRKC_MOUSE 411 0 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
MTAASRANP IRKC_HUMAN 1 1 -
MTAASRANP IRKC_RAT 1 1 -
MTAASRANP IRKI_HUMAN 1 1 -
MTAASRANP IRKC_MOUSE 1 1 -

Motif 2 width=8
Element Seqn Id St Int Rpt
IEFANMDE IRKC_HUMAN 55 45 -
IEFANMDE IRKC_RAT 55 45 -
IEFANMDE IRKI_HUMAN 55 45 -
IEFANMDE IRKC_MOUSE 55 45 -

Motif 3 width=9
Element Seqn Id St Int Rpt
PAEGRGRTP IRKC_HUMAN 114 51 -
PAEGRGRTP IRKC_RAT 114 51 -
PAEGRGRTP IRKI_HUMAN 114 51 -
PAEGRGRTP IRKC_MOUSE 114 51 -

Motif 4 width=9
Element Seqn Id St Int Rpt
VMQVHGFMA IRKC_HUMAN 124 1 -
VLQVHGFMA IRKC_RAT 124 1 -
VMQVHGFMA IRKI_HUMAN 124 1 -
VLQVHGFMA IRKC_MOUSE 124 1 -

Motif 5 width=9
Element Seqn Id St Int Rpt
IKPRVTEEG IRKC_HUMAN 233 100 -
IKPRVTEEG IRKC_RAT 233 100 -
IKPRVTKEG IRKI_HUMAN 233 100 -
IKPRVTEEG IRKC_MOUSE 233 100 -

Motif 6 width=8
Element Seqn Id St Int Rpt
QDLETDDF IRKC_HUMAN 285 43 -
QDLETDDF IRKC_RAT 286 44 -
QDLQMDDF IRKI_HUMAN 286 44 -
QDLETDDF IRKC_MOUSE 286 44 -

Motif 7 width=7
Element Seqn Id St Int Rpt
NQYKIDY IRKC_HUMAN 335 42 -
NQYKIDY IRKC_RAT 336 42 -
NQYKIDY IRKI_HUMAN 335 41 -
NQYKIDY IRKC_MOUSE 336 42 -

Motif 8 width=9
Element Seqn Id St Int Rpt
VENKFLLPS IRKC_HUMAN 362 20 -
VENKFLLPS IRKC_RAT 363 20 -
VENKFLLPR IRKI_HUMAN 362 20 -
VENKFLLPS IRKC_MOUSE 363 20 -

Motif 9 width=8
Element Seqn Id St Int Rpt
LAFLSRDE IRKC_HUMAN 380 9 -
LAFLSRDE IRKC_RAT 381 9 -
LAFLSRDE IRKI_HUMAN 380 9 -
LAFLIRDE IRKC_MOUSE 381 9 -

Motif 10 width=12
Element Seqn Id St Int Rpt
EDEADGDQDGRS IRKC_HUMAN 388 0 -
EDEVATDRDGRS IRKC_RAT 389 0 -
EDEADGDQDGRS IRKI_HUMAN 388 0 -
EDEVSTDRDVRT IRKC_MOUSE 389 0 -

Motif 11 width=10
Element Seqn Id St Int Rpt
PQARHDFDRL IRKC_HUMAN 405 5 -
PQPEHDFDRL IRKC_RAT 401 0 -
PQARHDFDRL IRKI_HUMAN 405 5 -
PQPEHDFDRL IRKC_MOUSE 401 0 -

Motif 12 width=8
Element Seqn Id St Int Rpt
QAGGGVLE IRKC_HUMAN 415 0 -
QASSGALE IRKC_RAT 411 0 -
QAGGGVLE IRKI_HUMAN 415 0 -
QASSAALV IRKC_MOUSE 411 0 -