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PR01324

Identifier
KIR21CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01324
No. of Motifs
6
Creation Date
11-MAY-2000
Title
Kir2.1 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).
 
4. WANG, H., YANG, B., ZHANG, L., XU, D. AND WANG, Z. 
Direct block of inward rectifier potassium channels by nicotine. 
TOXICOL.APPL.PHARMACOL. 164 97-101 (2000).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
Kir2.1 (also known as IRK1) is thought to play a role in controlling the
excitability of brain and heart tissues. Immuno-localisation studies have
revealed it to be present on many cell types within the forebrain, including
neurones, macroglia, endothelial, ependymal and vascular smooth muscle
cells. Phosphatidylinositol 4,5-bisphosphate (PIP2) is thought to interact
with Kir2.1 (as well as several other Kir subunits) via a C-terminal motif,
promoting channel activity. Recently it has been demonstrated that nicotine
can block Kir2.1 channels, this likely contributing to its ability to
promote the occurrence of cardiac arrhythmias [4].
 
KIR21CHANNEL is a 6-element fingerprint that provides a signature for the
Kir2.1 inward rectifier K+ channel. The fingerprint was derived from an
initial alignment of 8 sequences: the motifs were drawn from virtually the
the full alignment length, focusing on those sections that characterise
Kir2.1 but distinguish it from other subtypes - motifs 1-3 lie within the
putative cytoplasmic N-terminus; motif 4 lies between the first TM domain
and the pore-forming (H5) domain; and motifs 5-6 encode portions of the
cytoplasmic C-terminus. Two iterations on SPTR37_10f were required to reach
convergence, at which point a true set comprising 10 sequences was
identified.
Summary Information
10 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6101010101010
5000000
4000000
3000000
2000000
123456
True Positives
IRK2_CAVPO    IRK2_CHICK    IRK2_HUMAN    IRK2_MOUSE    
IRK2_PIG IRK2_RABIT IRK2_RAT O15110
O19182 O42229
Sequence Titles
IRK2_CAVPO  INWARD RECTIFIER POTASSIUM CHANNEL 2 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 2) (CARDIAC INWARD RECTIFIER POTASSIUM CHANNEL) (IRK1) (KIR2.1) - CAVIA PORCELLUS (GUINEA PIG). 
IRK2_CHICK INWARD RECTIFIER POTASSIUM CHANNEL 2 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 2) (IRK1) (KIR2.1) - GALLUS GALLUS (CHICKEN).
IRK2_HUMAN INWARD RECTIFIER POTASSIUM CHANNEL 2 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 2) (CARDIAC INWARD RECTIFIER POTASSIUM CHANNEL) (IRK1) (KIR2.1) - HOMO SAPIENS (HUMAN), AND MACACA MULATTA (RHESUS MACAQUE).
IRK2_MOUSE INWARD RECTIFIER POTASSIUM CHANNEL 2 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 2) (KIR2.1) - MUS MUSCULUS (MOUSE).
IRK2_PIG INWARD RECTIFIER POTASSIUM CHANNEL 2 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 2) (KIR2.1) - SUS SCROFA (PIG).
IRK2_RABIT INWARD RECTIFIER POTASSIUM CHANNEL 2 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 2) (KIR2.1) - ORYCTOLAGUS CUNICULUS (RABBIT).
IRK2_RAT INWARD RECTIFIER POTASSIUM CHANNEL 2 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 2) (RBL-IRK1) - RATTUS NORVEGICUS (RAT).
O15110 INWARDLY RECTIFYING POTASSIUM CHANNEL KIR 2.1 - HOMO SAPIENS (HUMAN).
O19182 INWARD RECTIFIER POTASSIUM CHANNEL - BOS TAURUS (BOVINE).
O42229 INWARD RECTIFIER POTASSIUM CHANNEL - GALLUS GALLUS (CHICKEN).
Scan History
SPTR37_10f 2  50   NSINGLE    
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
MGSVRTNRYS IRK2_CAVPO 1 1 -
MGSVRTNRYS IRK2_HUMAN 1 1 -
MGSVRTNRYS O15110 1 1 -
MGSVRTNRYS IRK2_RAT 1 1 -
MGSVRTNRYS IRK2_RABIT 1 1 -
MGSVRTNRYS IRK2_PIG 1 1 -
MGSVRTNRYS IRK2_MOUSE 1 1 -
MGSVRTNRYS IRK2_CHICK 1 1 -

Motif 2 width=8
Element Seqn Id St Int Rpt
LATMAVAN IRK2_CAVPO 21 10 -
LATMAVAN IRK2_HUMAN 21 10 -
LATMAVAN O15110 21 10 -
LATMAVAN IRK2_RAT 21 10 -
LATMAVAN IRK2_RABIT 21 10 -
LATLAVAN IRK2_PIG 21 10 -
LATMAVAN IRK2_MOUSE 21 10 -
LATMAVAN IRK2_CHICK 21 10 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SKVHTRQQCRS IRK2_CAVPO 35 6 -
SKVHTRQQCRS IRK2_HUMAN 35 6 -
SKVHTRQQCRS O15110 35 6 -
SKVHTRQQCRS IRK2_RAT 35 6 -
SKVHTRQQCRS IRK2_RABIT 35 6 -
SKVHTRQQCRS IRK2_PIG 35 6 -
SKVHTRQQCRS IRK2_MOUSE 35 6 -
SKVHTRQQCRS IRK2_CHICK 35 6 -

Motif 4 width=13
Element Seqn Id St Int Rpt
SKESKACVSEVNS IRK2_CAVPO 116 70 -
SKEGKACVSEVNS IRK2_HUMAN 116 70 -
SKEGKACVSEVNS O15110 116 70 -
SKESKACVSEVNS IRK2_RAT 116 70 -
SRESKACVSEVNS IRK2_RABIT 116 70 -
SKESKACVSEVNS IRK2_PIG 116 70 -
SKVSKACVSEVNS IRK2_MOUSE 116 70 -
QENNKPCVSQVSS IRK2_CHICK 116 70 -

Motif 5 width=12
Element Seqn Id St Int Rpt
ENGVPESTSTDT IRK2_CAVPO 391 262 -
ENGVPESTSTDT IRK2_HUMAN 391 262 -
ENGVPESTSTDT O15110 391 262 -
ENGVPESTSTDS IRK2_RAT 391 262 -
ENGVPESTSTDT IRK2_RABIT 391 262 -
ENGVPESTSTDT IRK2_PIG 391 262 -
ENGVPESTSTDS IRK2_MOUSE 392 263 -
DTGVPESTSTDT IRK2_CHICK 391 262 -

Motif 6 width=13
Element Seqn Id St Int Rpt
IDLHNQASVPLEP IRK2_CAVPO 406 3 -
IDLHNQASVPLEP IRK2_HUMAN 406 3 -
IDLHNQASVPLEP O15110 406 3 -
IDLHNQASVPLEP IRK2_RAT 406 3 -
IDLHNQASVPLEP IRK2_RABIT 406 3 -
IDLHNQASVPLEP IRK2_PIG 406 3 -
IDLHNQASVPLEP IRK2_MOUSE 407 3 -
MDHHNQAGVPLEP IRK2_CHICK 406 3 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
MGSVRTNRYS IRK2_CAVPO 1 1 -
MGSVRTNRYS IRK2_HUMAN 1 1 -
MGSVRTNRYS O15110 1 1 -
MGSVRTNRYS IRK2_RAT 1 1 -
MGSVRTNRYS IRK2_RABIT 1 1 -
MGSVRTNRYS IRK2_PIG 1 1 -
MGSVRTNRYS O19182 1 1 -
MGSVRTNRYS IRK2_MOUSE 1 1 -
MGSVRTNRYS IRK2_CHICK 1 1 -
MGSVRTNRYS O42229 1 1 -

Motif 2 width=8
Element Seqn Id St Int Rpt
LATMAVAN IRK2_CAVPO 21 10 -
LATMAVAN IRK2_HUMAN 21 10 -
LATMAVAN O15110 21 10 -
LATMAVAN IRK2_RAT 21 10 -
LATMAVAN IRK2_RABIT 21 10 -
LATLAVAN IRK2_PIG 21 10 -
LATLAVAN O19182 21 10 -
LATMAVAN IRK2_MOUSE 21 10 -
LATMAVAN IRK2_CHICK 21 10 -
LATMAVAN O42229 21 10 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SKVHTRQQCRS IRK2_CAVPO 35 6 -
SKVHTRQQCRS IRK2_HUMAN 35 6 -
SKVHTRQQCRS O15110 35 6 -
SKVHTRQQCRS IRK2_RAT 35 6 -
SKVHTRQQCRS IRK2_RABIT 35 6 -
SKVHTRQQCRS IRK2_PIG 35 6 -
SKVHTRQQCRS O19182 35 6 -
SKVHTRQQCRS IRK2_MOUSE 35 6 -
SKVHTRQQCRS IRK2_CHICK 35 6 -
SKVHTRQQCRS O42229 35 6 -

Motif 4 width=13
Element Seqn Id St Int Rpt
SKESKACVSEVNS IRK2_CAVPO 116 70 -
SKEGKACVSEVNS IRK2_HUMAN 116 70 -
SKEGKACVSEVNS O15110 116 70 -
SKESKACVSEVNS IRK2_RAT 116 70 -
SRESKACVSEVNS IRK2_RABIT 116 70 -
SKESKACVSEVNS IRK2_PIG 116 70 -
SKESKACVSEVNS O19182 116 70 -
SKVSKACVSEVNS IRK2_MOUSE 116 70 -
QENNKPCVSQVSS IRK2_CHICK 116 70 -
QENNKPCVSQVSS O42229 116 70 -

Motif 5 width=12
Element Seqn Id St Int Rpt
ENGVPESTSTDT IRK2_CAVPO 391 262 -
ENGVPESTSTDT IRK2_HUMAN 391 262 -
ENGVPESTSTDT O15110 391 262 -
ENGVPESTSTDS IRK2_RAT 391 262 -
ENGVPESTSTDT IRK2_RABIT 391 262 -
ENGVPESTSTDT IRK2_PIG 391 262 -
ENGVPESTSTDT O19182 391 262 -
ENGVPESTSTDS IRK2_MOUSE 392 263 -
DTGVPESTSTDT IRK2_CHICK 391 262 -
DTGVPESTSTDT O42229 391 262 -

Motif 6 width=13
Element Seqn Id St Int Rpt
IDLHNQASVPLEP IRK2_CAVPO 406 3 -
IDLHNQASVPLEP IRK2_HUMAN 406 3 -
IDLHNQASVPLEP O15110 406 3 -
IDLHNQASVPLEP IRK2_RAT 406 3 -
IDLHNQASVPLEP IRK2_RABIT 406 3 -
IDLHNQASVPLEP IRK2_PIG 406 3 -
IDLHNQASVPLEP O19182 406 3 -
IDLHNQASVPLEP IRK2_MOUSE 407 3 -
MDHHNQAGVPLEP IRK2_CHICK 406 3 -
MDHHNQAGVPLEP O42229 406 3 -