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PR01322

Identifier
KIR12CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01322
No. of Motifs
9
Creation Date
12-MAY-2000
Title
Kir1.2 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
PRODOM; PD029154
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).
 
4. MACGREGOR, G.G., XU, J.Z., MCNICHOLAS, C.M., GIEBISCH, G. AND
HEBERT, S.C. 
Partially active channels produced by PKA site mutation of the cloned
renal K+ channel, ROMK2 (kir1.2). 
AM.J.PHYSIOL. 275 F415-F422 (1998).
 
5. KURSCHNER, C., MERMELSTEIN, P.G., HOLDEN, W.T. AND SURMEIER D.J.
CIPP, a novel multivalent PDZ domain protein, selectively interacts with
Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins. 
MOL.CELL.NEUROSCIENCE 11 161-172 (1998).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
The Kir1.2 channel (also known as Kir4.1, ROMK2, BIR10, KAB-2 and BIRK1)
is found principally in the brain, where it is widely distributed. It has
also been found on satellite cells of the rat cochlear ganglia, and at
low levels within the kidney. Kir1.2 channel activity is dependent upon
phosphorylation-state, with two serine residues within the C-terminus,
likely the target of PKA protein kinase A, being involved [4]. Kir1.2 has
recently been shown to interact with a novel PDZ domain-containing protein,
CIPP [5].
 
KIR12CHANNEL is a 9-element fingerprint that provides a signature for the 
Kir1.2 inward rectifier K+ channel. The fingerprint was derived from an
initial alignment of 2 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise Kir1.2 but distinguish it from other subtypes -
motifs 1-3 encode portions of the putative cytoplasmic N-terminus; and
motifs 4-7 lie in the cytoplasmic C-terminus. A single iteration on 
SPTR37_10f was required to reach convergence, no further sequences being
identified beyond the starting set.
Summary Information
2 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9222222222
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
IRKA_HUMAN    IRKA_RAT      
Sequence Titles
IRKA_HUMAN  ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 10 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 10) (INWARD RECTIFIER POTASSIUM CHANNEL KIR1.2) (ATP-DEPENDENT INWARDLY RECTIFYING POTASSIUM CHANNEL KIR4.1) - HOMO SAPIENS (HUMAN). 
IRKA_RAT ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 10 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 10) (ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL KAB-2) (KIR4.1) (BIR10) (BRAIN- SPECIFIC INWARDLY RECTIFYING K+ CHANNEL 1) (BIRK1) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  30   NSINGLE    
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
MTSVAKVYYSQTTQ IRKA_HUMAN 1 1 -
MTSVAKVYYSQTTQ IRKA_RAT 1 1 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TESRPLMGPGI IRKA_HUMAN 15 0 -
TESRPLVAPGI IRKA_RAT 15 0 -

Motif 3 width=10
Element Seqn Id St Int Rpt
MEHIADKRFL IRKA_HUMAN 41 15 -
MEHIADKRFL IRKA_RAT 41 15 -

Motif 4 width=9
Element Seqn Id St Int Rpt
VVASHNGKP IRKA_HUMAN 186 135 -
VVAYHNGKL IRKA_RAT 186 135 -

Motif 5 width=8
Element Seqn Id St Int Rpt
ENIRLNQV IRKA_HUMAN 227 32 -
ENIRLNQV IRKA_RAT 227 32 -

Motif 6 width=9
Element Seqn Id St Int Rpt
TPAISLSAS IRKA_HUMAN 312 77 -
TPAISLSAS IRKA_RAT 312 77 -

Motif 7 width=9
Element Seqn Id St Int Rpt
DQVVKVASP IRKA_HUMAN 331 10 -
DQVVKVASP IRKA_RAT 331 10 -

Motif 8 width=10
Element Seqn Id St Int Rpt
GLRDSTVRYG IRKA_HUMAN 341 1 -
GLRDSTVRYG IRKA_RAT 341 1 -

Motif 9 width=9
Element Seqn Id St Int Rpt
QAEKEGSAL IRKA_HUMAN 364 13 -
QAEKEGSAL IRKA_RAT 364 13 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
MTSVAKVYYSQTTQ IRKA_HUMAN 1 1 -
MTSVAKVYYSQTTQ IRKA_RAT 1 1 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TESRPLMGPGI IRKA_HUMAN 15 0 -
TESRPLVAPGI IRKA_RAT 15 0 -

Motif 3 width=10
Element Seqn Id St Int Rpt
MEHIADKRFL IRKA_HUMAN 41 15 -
MEHIADKRFL IRKA_RAT 41 15 -

Motif 4 width=9
Element Seqn Id St Int Rpt
VVASHNGKP IRKA_HUMAN 186 135 -
VVAYHNGKL IRKA_RAT 186 135 -

Motif 5 width=8
Element Seqn Id St Int Rpt
ENIRLNQV IRKA_HUMAN 227 32 -
ENIRLNQV IRKA_RAT 227 32 -

Motif 6 width=9
Element Seqn Id St Int Rpt
TPAISLSAS IRKA_HUMAN 312 77 -
TPAISLSAS IRKA_RAT 312 77 -

Motif 7 width=9
Element Seqn Id St Int Rpt
DQVVKVASP IRKA_HUMAN 331 10 -
DQVVKVASP IRKA_RAT 331 10 -

Motif 8 width=10
Element Seqn Id St Int Rpt
GLRDSTVRYG IRKA_HUMAN 341 1 -
GLRDSTVRYG IRKA_RAT 341 1 -

Motif 9 width=9
Element Seqn Id St Int Rpt
QAEKEGSAL IRKA_HUMAN 364 13 -
QAEKEGSAL IRKA_RAT 364 13 -