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PR01314

Identifier
P2X7RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01314
No. of Motifs
11
Creation Date
23-MAR-2000
Title
P2X7 purinoceptor signature
Database References
PRINTS; PR00252 NRIONCHANNEL; PR01307 P2XRECEPTOR
PRODOM; PD041643
Literature References
1. NORTH, R.A.
Families of ion channels with two hydrophobic segments.
CURR.OPIN.CELL BIOL. 8 474-483 (1996).
 
2. MACKENZIE, A.B., SURPRENANT, A. AND NORTH, R.A.
Functional and molecular diversity of purinergic ion channel receptors.
ANN.N.Y.ACAD.SCI. 868 716-729 (1999).

Documentation
P2X purinoceptors are cell membrane ion channels, gated by adenosine
5'-triphosphate (ATP) and other nucleotides. They have been found to be
widely expressed on mammalian cells, and, by means of their functional 
properties, can be differentiated into three sub-groups. The first group is
almost equally well activated by ATP and its analogue alphabetamethyleneATP,
whereas, the second group is not activated by the latter compound. A third
type of receptor (also called P2Z) is distinguished by the fact that
repeated or prolonged agonist application leads to the opening of much
larger pores, allowing large molecules to traverse the cell membrane. This
increased permeability rapidly leads to cell death, and lysis.
 
Molecular cloning studies have identified seven P2X receptor subtypes,
designated P2X1-P2X7. These receptors are proteins that share 35-48% amino
acid identity, and possess two putative transmembrane (TM) domains,
separated by a long (~270 residues) intervening sequence, which is
thought to form an extracellular loop. Around 1/4 of the residues within
the loop are invariant between the cloned subtypes, including 10
characteristic cysteines.
 
Studies of the functional properties of heterologously expressed P2X
receptors, together with the examination of their distribution in native
tissues, suggests they likely occur as both homo- and heteromultimers
in vivo [1,2].
 
When the P2X7 receptor is expressed, it is found to have different
functional properties from those of P2X1-P2X6. Key properties of the current
produced are little rectification or desensitisation, and strong
potentiation of responses when the concentration of extracellular Ca2+
and/or Mg2+ are reduced. It is also found to be relatively insensitive to
ATP. In certain studies, prolonged activation of expressed P2X7 receptors
causes cell permeabilisation, and lysis, suggesting that the P2X7 receptor
may correspond to the native P2Z receptor, which responds similarly. 
 
P2X7RECEPTOR is an 11-element fingerprint that provides a signature for the
P2X purinoceptor isoform 7. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the P2X purinoceptor isoform 7 but distinguish it from
others - motif 1 resides within the putative cytoplasmic N-terminus; motif
2 encodes the C-terminal half of the first TM domain; motifs 3-4 lie within
the exoplasmic loop connecting the two TM domains; and motifs 5-11 encode
portions of the putative cytoplasmic C-terminus. Two iterations on
SPTR37_10f were required to reach convergence, at which point a true set
comprising 3 sequences was identified.
Summary Information
3 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
1133333333333
1000000000000
900000000000
800000000000
700000000000
600000000000
500000000000
400000000000
300000000000
200000000000
1234567891011
True Positives
P2X7_HUMAN    P2X7_RAT      Q9Z1M0        
Sequence Titles
P2X7_HUMAN  P2X PURINOCEPTOR 7 (ATP RECEPTOR) (P2X7) (PURINERGIC RECEPTOR) (P2Z RECEPTOR) - HOMO SAPIENS (HUMAN). 
P2X7_RAT P2X PURINOCEPTOR 7 (ATP RECEPTOR) (P2X7) (PURINERGIC RECEPTOR) (P2Z RECEPTOR) - RATTUS NORVEGICUS (RAT).
Q9Z1M0 P2X7 RECEPTOR SUBUNIT - MUS MUSCULUS (MOUSE).
Scan History
SPTR37_10f 2  30   NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
MPACCSWNDVFQ P2X7_RAT 1 1 -
MPACCSCSDVFQ P2X7_HUMAN 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
HMTVFSYVSFALMS P2X7_RAT 34 21 -
HVIIFSYVCFALVS P2X7_HUMAN 34 21 -

Motif 3 width=11
Element Seqn Id St Int Rpt
DKLYQRKEPLI P2X7_RAT 48 0 -
DKLYQRKEPVI P2X7_HUMAN 48 0 -

Motif 4 width=10
Element Seqn Id St Int Rpt
NIDFPGHNYT P2X7_RAT 195 136 -
NIDFPGHNYT P2X7_HUMAN 195 136 -

Motif 5 width=16
Element Seqn Id St Int Rpt
VNEYYYRKKCEPIVEP P2X7_RAT 379 174 -
VNEYYYRKKCESIVEP P2X7_HUMAN 379 174 -

Motif 6 width=12
Element Seqn Id St Int Rpt
KPTLKYVSFVDE P2X7_RAT 395 0 -
KPTLKYVSFVDE P2X7_HUMAN 395 0 -

Motif 7 width=15
Element Seqn Id St Int Rpt
HIWMVDQQLLGKSLQ P2X7_RAT 408 1 -
HIRMVNQQLLGRSLQ P2X7_HUMAN 408 1 -

Motif 8 width=14
Element Seqn Id St Int Rpt
SPPIPGQPEEMQLL P2X7_RAT 449 26 -
TPPIPGQPEEIQLL P2X7_HUMAN 449 26 -

Motif 9 width=14
Element Seqn Id St Int Rpt
LCCRRKPGQCITTS P2X7_RAT 497 34 -
LCCRKKPGACITTS P2X7_HUMAN 497 34 -

Motif 10 width=17
Element Seqn Id St Int Rpt
SQDMADFAILPSCCRWK P2X7_RAT 560 49 -
SQDMADFAILPSCCRWR P2X7_HUMAN 560 49 -

Motif 11 width=16
Element Seqn Id St Int Rpt
IRKEFPKTQGQYSGFK P2X7_RAT 577 0 -
IRKEFPKSEGQYSGFK P2X7_HUMAN 577 0 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
MPACCSWNDVFQ Q9Z1M0 1 1 -
MPACCSWNDVFQ P2X7_RAT 1 1 -
MPACCSCSDVFQ P2X7_HUMAN 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
HMIVFSYISFALVS Q9Z1M0 34 21 -
HMTVFSYVSFALMS P2X7_RAT 34 21 -
HVIIFSYVCFALVS P2X7_HUMAN 34 21 -

Motif 3 width=11
Element Seqn Id St Int Rpt
DKLYQRKEPVI Q9Z1M0 48 0 -
DKLYQRKEPLI P2X7_RAT 48 0 -
DKLYQRKEPVI P2X7_HUMAN 48 0 -

Motif 4 width=10
Element Seqn Id St Int Rpt
NIHFPGHNYT Q9Z1M0 195 136 -
NIDFPGHNYT P2X7_RAT 195 136 -
NIDFPGHNYT P2X7_HUMAN 195 136 -

Motif 5 width=16
Element Seqn Id St Int Rpt
VNEYYYRKKCESIMEP Q9Z1M0 379 174 -
VNEYYYRKKCEPIVEP P2X7_RAT 379 174 -
VNEYYYRKKCESIVEP P2X7_HUMAN 379 174 -

Motif 6 width=12
Element Seqn Id St Int Rpt
KPTLKYVSFVDE Q9Z1M0 395 0 -
KPTLKYVSFVDE P2X7_RAT 395 0 -
KPTLKYVSFVDE P2X7_HUMAN 395 0 -

Motif 7 width=15
Element Seqn Id St Int Rpt
HIRMVDQQLLGKSLQ Q9Z1M0 408 1 -
HIWMVDQQLLGKSLQ P2X7_RAT 408 1 -
HIRMVNQQLLGRSLQ P2X7_HUMAN 408 1 -

Motif 8 width=14
Element Seqn Id St Int Rpt
SPLTPGQSEEIQLL Q9Z1M0 449 26 -
SPPIPGQPEEMQLL P2X7_RAT 449 26 -
TPPIPGQPEEIQLL P2X7_HUMAN 449 26 -

Motif 9 width=14
Element Seqn Id St Int Rpt
LCCRRKPGRCITTS Q9Z1M0 497 34 -
LCCRRKPGQCITTS P2X7_RAT 497 34 -
LCCRKKPGACITTS P2X7_HUMAN 497 34 -

Motif 10 width=17
Element Seqn Id St Int Rpt
SQDMADFAILPSCCRWR Q9Z1M0 560 49 -
SQDMADFAILPSCCRWK P2X7_RAT 560 49 -
SQDMADFAILPSCCRWR P2X7_HUMAN 560 49 -

Motif 11 width=16
Element Seqn Id St Int Rpt
IRKEFPKTEGQYSGFK Q9Z1M0 577 0 -
IRKEFPKTQGQYSGFK P2X7_RAT 577 0 -
IRKEFPKSEGQYSGFK P2X7_HUMAN 577 0 -