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PR01312

Identifier
P2X5RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01312
No. of Motifs
4
Creation Date
21-MAR-2000
Title
P2X5 purinoceptor signature
Database References
PRINTS; PR00252 NRIONCHANNEL; PR01307 P2XRECEPTOR
Literature References
1. NORTH, R.A.
Families of ion channels with two hydrophobic segments.
CURR.OPIN.CELL BIOL. 8 474-483 (1996).
 
2. MACKENZIE, A.B., SURPRENANT, A. AND NORTH, R.A.
Functional and molecular diversity of purinergic ion channel receptors.
ANN.N.Y.ACAD.SCI. 868 716-729 (1999).

Documentation
P2X purinoceptors are cell membrane ion channels, gated by adenosine
5'-triphosphate (ATP) and other nucleotides. They have been found to be
widely expressed on mammalian cells, and, by means of their functional 
properties, can be differentiated into three sub-groups. The first group is
almost equally well activated by ATP and its analogue alphabetamethyleneATP,
whereas, the second group is not activated by the latter compound. A third
type of receptor (also called P2Z) is distinguished by the fact that
repeated or prolonged agonist application leads to the opening of much
larger pores, allowing large molecules to traverse the cell membrane. This
increased permeability rapidly leads to cell death, and lysis.
 
Molecular cloning studies have identified seven P2X receptor subtypes,
designated P2X1-P2X7. These receptors are proteins that share 35-48% amino
acid identity, and possess two putative transmembrane (TM) domains,
separated by a long (~270 residues) intervening sequence, which is
thought to form an extracellular loop. Around 1/4 of the residues within
the loop are invariant between the cloned subtypes, including 10
characteristic cysteines.
 
Studies of the functional properties of heterologously expressed P2X
receptors, together with the examination of their distribution in native
tissues, suggests they likely occur as both homo- and heteromultimers
in vivo [1,2].
 
The P2X5 receptor (along with P2X2, P2X4 and P2X6) falls into a group of
receptors that are sensitive to ATP, but not alphabetamethyleneATP. Splice
variants of P2X5 have been detected [2].
 
P2X5RECEPTOR is a 4-element fingerprint that provides a signature for the
P2X purinoceptor isoform 5. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the P2X purinoceptor isoform 5 but distinguish it from
others - motif 1 resides within the putative cytoplasmic N-terminus; and
motifs 2-4 lie within the exoplasmic loop connecting the two putative TM
domains. Two iterations on SPTR37_10f were required to reach convergence,
at which point a true set comprising 3 sequences was identified.
Summary Information
3 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
43333
30000
20000
1234
True Positives
O75540        P2X5_HUMAN    P2X5_RAT      
Sequence Titles
O75540      IONOTROPIC ATP RECEPTOR P2X5B - HOMO SAPIENS (HUMAN). 
P2X5_HUMAN P2X PURINOCEPTOR 5 (ATP RECEPTOR) (P2X5) (PURINERGIC RECEPTOR) - HOMO SAPIENS (HUMAN).
P2X5_RAT P2X PURINOCEPTOR 5 (ATP RECEPTOR) (P2X5) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
MGQAGCKGLCLSL P2X5_HUMAN 1 1 -
MGQAAWKGFVLSL P2X5_RAT 1 1 -

Motif 2 width=11
Element Seqn Id St Int Rpt
VDTSLQSAVIT P2X5_HUMAN 58 44 -
IDTSLQSAVVT P2X5_RAT 58 44 -

Motif 3 width=10
Element Seqn Id St Int Rpt
AENEGIPDGA P2X5_HUMAN 119 50 -
AEREGIPDGE P2X5_RAT 119 50 -

Motif 4 width=9
Element Seqn Id St Int Rpt
LIKKREFYR P2X5_HUMAN 339 210 -
LIRKSEFYR P2X5_RAT 361 232 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
MGQAGCKGLCLSL P2X5_HUMAN 1 1 -
MGQAGCKGLCLSL O75540 1 1 -
MGQAAWKGFVLSL P2X5_RAT 1 1 -

Motif 2 width=11
Element Seqn Id St Int Rpt
VDTSLQSAVIT P2X5_HUMAN 58 44 -
VDTSLQSAVIT O75540 58 44 -
IDTSLQSAVVT P2X5_RAT 58 44 -

Motif 3 width=10
Element Seqn Id St Int Rpt
AENEGIPDGA P2X5_HUMAN 119 50 -
AQNEGIPDGA O75540 95 26 -
AEREGIPDGE P2X5_RAT 119 50 -

Motif 4 width=9
Element Seqn Id St Int Rpt
LIKKREFYR P2X5_HUMAN 339 210 -
LIKKREFYR O75540 314 209 -
LIRKSEFYR P2X5_RAT 361 232 -