SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01311

Identifier
P2X4RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01311
No. of Motifs
10
Creation Date
22-MAR-2000
Title
P2X4 purinoceptor signature
Database References
PRINTS; PR00252 NRIONCHANNEL; PR01307 P2XRECEPTOR
Literature References
1. NORTH, R.A.
Families of ion channels with two hydrophobic segments.
CURR.OPIN.CELL BIOL. 8 474-483 (1996).
 
2. MACKENZIE, A.B., SURPRENANT, A. AND NORTH, R.A.
Functional and molecular diversity of purinergic ion channel receptors.
ANN.N.Y.ACAD.SCI. 868 716-729 (1999).

Documentation
P2X purinoceptors are cell membrane ion channels, gated by adenosine
5'-triphosphate (ATP) and other nucleotides. They have been found to be
widely expressed on mammalian cells, and, by means of their functional 
properties, can be differentiated into three sub-groups. The first group is
almost equally well activated by ATP and its analogue alphabetamethyleneATP,
whereas, the second group is not activated by the latter compound. A third
type of receptor (also called P2Z) is distinguished by the fact that
repeated or prolonged agonist application leads to the opening of much
larger pores, allowing large molecules to traverse the cell membrane. This
increased permeability rapidly leads to cell death, and lysis.
 
Molecular cloning studies have identified seven P2X receptor subtypes,
designated P2X1-P2X7. These receptors are proteins that share 35-48% amino
acid identity, and possess two putative transmembrane (TM) domains,
separated by a long (~270 residues) intervening sequence, which is
thought to form an extracellular loop. Around 1/4 of the residues within
the loop are invariant between the cloned subtypes, including 10
characteristic cysteines.
 
Studies of the functional properties of heterologously expressed P2X
receptors, together with the examination of their distribution in native
tissues, suggests they likely occur as both homo- and heteromultimers
in vivo [1,2].
 
The P2X4 receptor (along with P2X2, P2X5 and P2X6) falls into a group of
receptors that are sensitive to ATP, but not alphabetamethyleneATP. There
is some evidence that P2X4 may heteropolymerise with P2X6, since they are
often found together in native tissues, and can be co-immunoprecipitated. 
Splice variants of the P2X4 receptor have been detected [2].
 
P2X4RECEPTOR is a 10-element fingerprint that provides a signature for the
P2X purinoceptor isoform 4. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the P2X purinoceptor isoform 4 but distinguish it from
others - motifs 1-2 reside within the putative cytoplasmic N-terminus;
motifs 3-4 encode portions of the first TM domain; motifs 5-8 lie within
the putative exoplasmic loop connecting the two TM domains; and motifs 9-10
lie on the cytoplasmic C-terminus. A single iteration on SPTR37_10f was
required to reach convergence, no further sequences being identified
beyond the starting set.
Summary Information
5 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
105555555555
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
P2X4_HUMAN    P2X4_RAT      Q9YI70        Q9Z256        
Q9Z257
Sequence Titles
P2X4_HUMAN  P2X PURINOCEPTOR 4 (ATP RECEPTOR) (P2X4) (PURINERGIC RECEPTOR) - HOMO SAPIENS (HUMAN). 
P2X4_RAT P2X PURINOCEPTOR 4 (ATP RECEPTOR) (P2X4) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
Q9YI70 P2X ATP RECEPTOR - GALLUS GALLUS (CHICKEN).
Q9Z256 ATP RECEPTOR P2X4 SUBUNIT ISOFORM A - MUS MUSCULUS (MOUSE).
Q9Z257 ATP RECEPTOR P2X4 SUBUNIT - MUS MUSCULUS (MOUSE).
Scan History
SPTR37_10f 1  50   NSINGLE    
Initial Motifs
Motif 1  width=8
Element Seqn Id St Int Rpt
MAGCCSAL P2X4_HUMAN 1 1 -
MAGCCSVL P2X4_RAT 1 1 -

Motif 2 width=5
Element Seqn Id St Int Rpt
RIVLI P2X4_HUMAN 19 10 -
RIVLI P2X4_RAT 19 10 -

Motif 3 width=9
Element Seqn Id St Int Rpt
LMNRAVQLL P2X4_RAT 30 6 -
LMNRAVQLL P2X4_HUMAN 30 6 -

Motif 4 width=7
Element Seqn Id St Int Rpt
VWEKGYQ P2X4_RAT 49 10 -
VWEKGYQ P2X4_HUMAN 49 10 -

Motif 5 width=8
Element Seqn Id St Int Rpt
THSNGVST P2X4_HUMAN 139 83 -
THSSGVAT P2X4_RAT 139 83 -

Motif 6 width=9
Element Seqn Id St Int Rpt
NITTTYLKS P2X4_HUMAN 208 61 -
NITTSYLKS P2X4_RAT 208 61 -

Motif 7 width=11
Element Seqn Id St Int Rpt
DTRDVEHNVSP P2X4_HUMAN 280 63 -
DTRDLEHNVSP P2X4_RAT 280 63 -

Motif 8 width=10
Element Seqn Id St Int Rpt
LAGNEQRTLI P2X4_HUMAN 303 12 -
LAGKEQRTLT P2X4_RAT 303 12 -

Motif 9 width=8
Element Seqn Id St Int Rpt
YCMKKRLY P2X4_HUMAN 359 46 -
YCMKKKYY P2X4_RAT 359 46 -

Motif 10 width=10
Element Seqn Id St Int Rpt
YVEDYEQGLA P2X4_HUMAN 374 7 -
YVEDYEQGLS P2X4_RAT 374 7 -
Final Motifs
Motif 1  width=8
Element Seqn Id St Int Rpt
MAGCCSVL P2X4_RAT 1 1 -
MAACCGAV Q9YI70 1 1 -
MAGCCSAL P2X4_HUMAN 1 1 -
MAGCCSVL Q9Z256 1 1 -
MAGCCSVL Q9Z257 1 1 -

Motif 2 width=5
Element Seqn Id St Int Rpt
RIVLI Q9Z257 19 10 -
RIVLI Q9YI70 19 10 -
RIVLI P2X4_HUMAN 19 10 -
RIVLI Q9Z256 19 10 -
RIVLI P2X4_RAT 19 10 -

Motif 3 width=9
Element Seqn Id St Int Rpt
LMNRVVQLL Q9Z257 30 6 -
LINRAVQLA Q9YI70 30 6 -
LMNRAVQLL P2X4_HUMAN 30 6 -
LMNRVVQLL Q9Z256 30 6 -
LMNRAVQLL P2X4_RAT 30 6 -

Motif 4 width=7
Element Seqn Id St Int Rpt
VWEKGYQ P2X4_RAT 49 10 -
LWEKGYQ Q9YI70 49 10 -
VWEKGYQ P2X4_HUMAN 49 10 -
VWEKGYQ Q9Z256 49 10 -
VWEKGYQ Q9Z257 49 10 -

Motif 5 width=8
Element Seqn Id St Int Rpt
THSNGVST P2X4_HUMAN 139 83 -
THSSGIGT Q9Z256 139 83 -
THSSGIGT Q9Z257 139 83 -
THSSGVAT P2X4_RAT 139 83 -
THSNGIQT Q9YI70 139 83 -

Motif 6 width=9
Element Seqn Id St Int Rpt
NITTTYLKS P2X4_HUMAN 208 61 -
NITTSYLKS Q9Z256 181 34 -
NITTSYLKS Q9Z257 208 61 -
NITTSYLKS P2X4_RAT 208 61 -
TFSSSYLKN Q9YI70 208 61 -

Motif 7 width=11
Element Seqn Id St Int Rpt
DTRDVEHNVSP P2X4_HUMAN 280 63 -
DTRDLEHNVSP Q9Z256 253 63 -
DTRDLEHNVSP Q9Z257 280 63 -
DTRDLEHNVSP P2X4_RAT 280 63 -
DNKDSANTISP Q9YI70 280 63 -

Motif 8 width=10
Element Seqn Id St Int Rpt
LAGNEQRTLI P2X4_HUMAN 303 12 -
LAGNEQRTLT Q9Z256 276 12 -
LAGNEQRTLT Q9Z257 303 12 -
LAGKEQRTLT P2X4_RAT 303 12 -
SSGIETRTLI Q9YI70 303 12 -

Motif 9 width=8
Element Seqn Id St Int Rpt
YCMKKRLY P2X4_HUMAN 359 46 -
YCMKKRYY Q9Z256 332 46 -
YCMKKRYY Q9Z257 359 46 -
YCMKKKYY P2X4_RAT 359 46 -
YCMKKRYF Q9YI70 359 46 -

Motif 10 width=10
Element Seqn Id St Int Rpt
YVEDYEQGLA P2X4_HUMAN 374 7 -
YVEDYEQGLS Q9Z256 347 7 -
YVEDYEQGLS Q9Z257 374 7 -
YVEDYEQGLS P2X4_RAT 374 7 -
YVEDYELGTS Q9YI70 374 7 -