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PR01310

Identifier
P2X3RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01310
No. of Motifs
9
Creation Date
21-MAR-2000
Title
P2X3 purinoceptor signature
Database References
PRINTS; PR00252 NRIONCHANNEL; PR01307 P2XRECEPTOR
PRODOM; PD041638
Literature References
1. NORTH, R.A.
Families of ion channels with two hydrophobic segments.
CURR.OPIN.CELL BIOL. 8 474-483 (1996).
 
2. MACKENZIE, A.B., SURPRENANT, A. AND NORTH, R.A.
Functional and molecular diversity of purinergic ion channel receptors.
ANN.N.Y.ACAD.SCI. 868 716-729 (1999).

Documentation
P2X purinoceptors are cell membrane ion channels, gated by adenosine
5'-triphosphate (ATP) and other nucleotides. They have been found to be
widely expressed on mammalian cells, and, by means of their functional 
properties, can be differentiated into three sub-groups. The first group is
almost equally well activated by ATP and its analogue alphabetamethyleneATP,
whereas, the second group is not activated by the latter compound. A third
type of receptor (also called P2Z) is distinguished by the fact that
repeated or prolonged agonist application leads to the opening of much
larger pores, allowing large molecules to traverse the cell membrane. This
increased permeability rapidly leads to cell death, and lysis.
 
Molecular cloning studies have identified seven P2X receptor subtypes,
designated P2X1-P2X7. These receptors are proteins that share 35-48% amino
acid identity, and possess two putative transmembrane (TM) domains,
separated by a long (~270 residues) intervening sequence, which is
thought to form an extracellular loop. Around 1/4 of the residues within
the loop are invariant between the cloned subtypes, including 10
characteristic cysteines.
 
Studies of the functional properties of heterologously expressed P2X
receptors, together with the examination of their distribution in native
tissues, suggests they likely occur as both homo- and heteromultimers
in vivo [1,2].
 
When expressed, P2X3 receptors (like P2X1 receptors) are found to be
equally well activated by alphabetamethyleneATP and ATP. P2X3 receptors
desensitise rapidly, with the decay being best-fit by two exponentials,
with time constants of ~50 and 300 ms [2].
 
P2X3RECEPTOR is a 9-element fingerprint that provides a signature for the
P2X purinoceptor isoform 3. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the P2X purinoceptor isoform 3 but distinguish it from
others - motifs 1-7 lie within the exoplasmic loop connecting the two
putative TM domains; and motifs 8-9 encode portions of the cytoplasmic
C-terminus. A single iteration on SPTR37_10f was required to reach
convergence, no further sequences being identified beyond the starting set.
Summary Information
2 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9222222222
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
P2X3_HUMAN    P2X3_RAT      
Sequence Titles
P2X3_HUMAN  P2X PURINOCEPTOR 3 (ATP RECEPTOR) (P2X3) (PURINERGIC RECEPTOR) - HOMO SAPIENS (HUMAN). 
P2X3_RAT P2X PURINOCEPTOR 3 (ATP RECEPTOR) (P2X3) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
GLYANRVMDVS P2X3_HUMAN 68 68 -
GRYANRVMDVS P2X3_RAT 68 68 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TSVFVIITKMI P2X3_HUMAN 87 8 -
TSVFVIITKMI P2X3_RAT 87 8 -

Motif 3 width=10
Element Seqn Id St Int Rpt
CRFHPDKDPF P2X3_HUMAN 203 105 -
CRFHPEKAPF P2X3_RAT 203 105 -

Motif 4 width=10
Element Seqn Id St Int Rpt
KFAGQDFAKL P2X3_HUMAN 223 10 -
KFAGQDFAKL P2X3_RAT 223 10 -

Motif 5 width=12
Element Seqn Id St Int Rpt
LDSVSEKSSVSP P2X3_HUMAN 265 32 -
LDGVSEKSSVSP P2X3_RAT 265 32 -

Motif 6 width=10
Element Seqn Id St Int Rpt
KMENGSEYRT P2X3_HUMAN 287 10 -
KMENGSEYRT P2X3_RAT 287 10 -

Motif 7 width=11
Element Seqn Id St Int Rpt
ISSVAAFTSVG P2X3_HUMAN 323 26 -
ISSVAAFTSVG P2X3_RAT 323 26 -

Motif 8 width=14
Element Seqn Id St Int Rpt
ETTLKIAALTNPVY P2X3_HUMAN 363 29 -
ETTLKGTASTNPVF P2X3_RAT 363 29 -

Motif 9 width=20
Element Seqn Id St Int Rpt
SDQTTAEKQSTDSGAFSIGH P2X3_HUMAN 378 1 -
SDQATVEKQSTDSGAYSIGH P2X3_RAT 378 1 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
GLYANRVMDVS P2X3_HUMAN 68 68 -
GRYANRVMDVS P2X3_RAT 68 68 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TSVFVIITKMI P2X3_HUMAN 87 8 -
TSVFVIITKMI P2X3_RAT 87 8 -

Motif 3 width=10
Element Seqn Id St Int Rpt
CRFHPDKDPF P2X3_HUMAN 203 105 -
CRFHPEKAPF P2X3_RAT 203 105 -

Motif 4 width=10
Element Seqn Id St Int Rpt
KFAGQDFAKL P2X3_HUMAN 223 10 -
KFAGQDFAKL P2X3_RAT 223 10 -

Motif 5 width=12
Element Seqn Id St Int Rpt
LDSVSEKSSVSP P2X3_HUMAN 265 32 -
LDGVSEKSSVSP P2X3_RAT 265 32 -

Motif 6 width=10
Element Seqn Id St Int Rpt
KMENGSEYRT P2X3_HUMAN 287 10 -
KMENGSEYRT P2X3_RAT 287 10 -

Motif 7 width=11
Element Seqn Id St Int Rpt
ISSVAAFTSVG P2X3_HUMAN 323 26 -
ISSVAAFTSVG P2X3_RAT 323 26 -

Motif 8 width=14
Element Seqn Id St Int Rpt
ETTLKIAALTNPVY P2X3_HUMAN 363 29 -
ETTLKGTASTNPVF P2X3_RAT 363 29 -

Motif 9 width=20
Element Seqn Id St Int Rpt
SDQTTAEKQSTDSGAFSIGH P2X3_HUMAN 378 1 -
SDQATVEKQSTDSGAYSIGH P2X3_RAT 378 1 -