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PR01307

Identifier
P2XRECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01307
No. of Motifs
5
Creation Date
01-NOV-1999
Title
P2X purinoceptor family signature
Database References
PRINTS; PR00252 NRIONCHANNEL; PR01308 P2X1RECEPTOR; PR01309 P2X2RECEPTOR
PRINTS; PR01310 P2X3RECEPTOR; PR01311 P2X4RECEPTOR; PR01312 P2X5RECEPTOR
PRINTS; PR01313 P2X6RECEPTOR; PR01314 P2X7RECEPTOR
PROSITE; PS01212 P2X_RECEPTOR
PFAM; PF00864 P2X_receptor
PRODOM; PD002383
Literature References
1. NORTH, R.A.
Families of ion channels with two hydrophobic segments.
CURR.OPIN.CELL BIOL. 8 474-483 (1996).
 
2. MACKENZIE, A.B., SURPRENANT, A. AND NORTH, R.A.
Functional and molecular diversity of purinergic ion channel receptors.
ANN.N.Y.ACAD.SCI. 868 716-729 (1999).

Documentation
P2X purinoceptors are cell membrane ion channels, gated by adenosine
5'-triphosphate (ATP) and other nucleotides. They have been found to be
widely expressed on mammalian cells, and, by means of their functional 
properties, can be differentiated into three sub-groups. The first group is
almost equally well activated by ATP and its analogue alphabetamethyleneATP,
whereas, the second group is not activated by the latter compound. A third
type of receptor (also called P2Z) is distinguished by the fact that
repeated or prolonged agonist application leads to the opening of much
larger pores, allowing large molecules to traverse the cell membrane. This
increased permeability rapidly leads to cell death, and lysis.
 
Molecular cloning studies have identified seven P2X receptor subtypes,
designated P2X1-P2X7. These receptors are proteins that share 35-48% amino
acid identity, and possess two putative transmembrane (TM) domains,
separated by a long (~270 residues) intervening sequence, which is
thought to form an extracellular loop. Around 1/4 of the residues within
the loop are invariant between the cloned subtypes, including 10
characteristic cysteines.
 
Studies of the functional properties of heterologously expressed P2X
receptors, together with the examination of their distribution in native
tissues, suggests they likely occur as both homo- and heteromultimers
in vivo [1,2].
 
P2XRECEPTOR is a 5-element fingerprint that provides a signature for the
P2X purinoceptor family. The fingerprint was derived from an initial
alignment of 16 sequences: the motifs were drawn from conserved regions
within the putative extracellular loop, connecting the two predicted TM
domains. Two iterations on SPTR37_10f were required to reach convergence,
at which point a true set comprising 23 sequences was identified.
Summary Information
23 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
52323232323
400000
300000
200000
12345
True Positives
O54869        O70399        O75540        P2X1_HUMAN    
P2X1_MOUSE P2X1_RAT P2X2_CAVPO P2X2_RAT
P2X3_HUMAN P2X3_RAT P2X4_HUMAN P2X4_RAT
P2X5_HUMAN P2X5_RAT P2X6_HUMAN P2X6_MOUSE
P2X6_RAT P2X7_HUMAN P2X7_RAT Q9YI70
Q9Z1M0 Q9Z256 Q9Z257
Sequence Titles
O54869      P2X PURINOCEPTOR 2 (ATP RECEPTOR) (P2X2) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT). 
O70399 P2X2 RECEPTOR SPLICE VARIANT P2X2-3 - CAVIA PORCELLUS (GUINEA PIG).
O75540 IONOTROPIC ATP RECEPTOR P2X5B - HOMO SAPIENS (HUMAN).
P2X1_HUMAN P2X PURINOCEPTOR 1 (ATP RECEPTOR) (P2X1) (PURINERGIC RECEPTOR) - HOMO SAPIENS (HUMAN).
P2X1_MOUSE P2X PURINOCEPTOR 1 (ATP RECEPTOR) (P2X1) (PURINERGIC RECEPTOR) - MUS MUSCULUS (MOUSE).
P2X1_RAT P2X PURINOCEPTOR 1 (ATP RECEPTOR) (P2X1) (PURINERGIC RECEPTOR) (RP-2 PROTEIN) - RATTUS NORVEGICUS (RAT).
P2X2_CAVPO P2X PURINOCEPTOR 2 (ATP RECEPTOR) (P2X2) (PURINERGIC RECEPTOR) - CAVIA PORCELLUS (GUINEA PIG).
P2X2_RAT P2X PURINOCEPTOR 2 (ATP RECEPTOR) (P2X2) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
P2X3_HUMAN P2X PURINOCEPTOR 3 (ATP RECEPTOR) (P2X3) (PURINERGIC RECEPTOR) - HOMO SAPIENS (HUMAN).
P2X3_RAT P2X PURINOCEPTOR 3 (ATP RECEPTOR) (P2X3) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
P2X4_HUMAN P2X PURINOCEPTOR 4 (ATP RECEPTOR) (P2X4) (PURINERGIC RECEPTOR) - HOMO SAPIENS (HUMAN).
P2X4_RAT P2X PURINOCEPTOR 4 (ATP RECEPTOR) (P2X4) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
P2X5_HUMAN P2X PURINOCEPTOR 5 (ATP RECEPTOR) (P2X5) (PURINERGIC RECEPTOR) - HOMO SAPIENS (HUMAN).
P2X5_RAT P2X PURINOCEPTOR 5 (ATP RECEPTOR) (P2X5) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
P2X6_HUMAN P2X PURINOCEPTOR 6 (ATP RECEPTOR) (P2X6) (PURINERGIC RECEPTOR) (P2XM) - HOMO SAPIENS (HUMAN).
P2X6_MOUSE P2X PURINOCEPTOR 6 (ATP RECEPTOR) (P2X6) (PURINERGIC RECEPTOR) (P2XM) - MUS MUSCULUS (MOUSE).
P2X6_RAT P2X PURINOCEPTOR 6 (ATP RECEPTOR) (P2X6) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
P2X7_HUMAN P2X PURINOCEPTOR 7 (ATP RECEPTOR) (P2X7) (PURINERGIC RECEPTOR) (P2Z RECEPTOR) - HOMO SAPIENS (HUMAN).
P2X7_RAT P2X PURINOCEPTOR 7 (ATP RECEPTOR) (P2X7) (PURINERGIC RECEPTOR) (P2Z RECEPTOR) - RATTUS NORVEGICUS (RAT).
Q9YI70 P2X ATP RECEPTOR - GALLUS GALLUS (CHICKEN).
Q9Z1M0 P2X7 RECEPTOR SUBUNIT - MUS MUSCULUS (MOUSE).
Q9Z256 ATP RECEPTOR P2X4 SUBUNIT ISOFORM A - MUS MUSCULUS (MOUSE).
Q9Z257 ATP RECEPTOR P2X4 SUBUNIT - MUS MUSCULUS (MOUSE).
Scan History
SPTR37_10f 2  200  NSINGLE    
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
RIWDVADYV P2X4_HUMAN 82 82 -
RIWDVADYV P2X4_RAT 82 82 -
RIWDVADYV P2X5_HUMAN 84 84 -
RLWDVADFV P2X5_RAT 84 84 -
KVWDVEEYV P2X2_RAT 79 79 -
KVWDVEEYV P2X2_CAVPO 87 87 -
RVMDVSDYV P2X3_HUMAN 73 73 -
RVMDVSDYV P2X3_RAT 73 73 -
QVWDVADYV P2X1_MOUSE 83 83 -
QVWDVADYV P2X1_HUMAN 83 83 -
QVWDVADYV P2X1_RAT 83 83 -
GIFDTADYT P2X7_RAT 86 86 -
RLWDVADFV P2X6_RAT 85 85 -
SVFDTADYT P2X7_HUMAN 86 86 -
RLWDVADFV P2X6_MOUSE 85 85 -
RLWDVADFV P2X6_HUMAN 83 83 -

Motif 2 width=12
Element Seqn Id St Int Rpt
KTCEVAAWCPVE P2X4_HUMAN 157 66 -
KTCEVAAWCPVE P2X4_RAT 157 66 -
GTCEIFAWCPLE P2X5_HUMAN 161 68 -
GTCEIFAWCPVE P2X5_RAT 161 68 -
KTCEVSAWCPVE P2X2_RAT 156 68 -
KTCEVSGWCPVE P2X2_CAVPO 164 68 -
RTCEIQGWCPTE P2X3_HUMAN 145 63 -
RTCEIQGWCPTE P2X3_RAT 145 63 -
KTCEIFGWCPVE P2X1_MOUSE 157 65 -
KTCEIFGWCPVE P2X1_HUMAN 157 65 -
KTCEIFGWCPVE P2X1_RAT 157 65 -
KTCEIFAWCPAE P2X7_RAT 160 65 -
RTCEIWSWCPVE P2X6_RAT 161 67 -
KTCEVSAWCPIE P2X7_HUMAN 160 65 -
RTCEIWSWCPVE P2X6_MOUSE 161 67 -
RTCEIWSWCPVE P2X6_HUMAN 159 67 -

Motif 3 width=13
Element Seqn Id St Int Rpt
FQDMAVEGGIMGI P2X4_HUMAN 243 74 -
FQEMAVEGGIMGI P2X4_RAT 243 74 -
FQDIALRGGVIGI P2X5_HUMAN 245 72 -
FQDIALKGGVIGI P2X5_RAT 245 72 -
FTELAHKGGVIGV P2X2_RAT 240 72 -
FTELAHRGGVIGV P2X2_CAVPO 248 72 -
FAKLARTGGVLGI P2X3_HUMAN 229 72 -
FAKLARTGGVLGI P2X3_RAT 229 72 -
FRSLAEKGGVVGI P2X1_MOUSE 243 74 -
FSTLAEKGGVVGI P2X1_HUMAN 243 74 -
FRSLAEKGGVVGI P2X1_RAT 243 74 -
FTEVAVQGGIMGI P2X7_RAT 242 70 -
FEDLALLGGAVGI P2X6_RAT 246 73 -
FSDVAIQGGIMGI P2X7_HUMAN 242 70 -
FEDLALLGGAVGI P2X6_MOUSE 246 73 -
FEDLALLGGSVGI P2X6_HUMAN 244 73 -

Motif 4 width=11
Element Seqn Id St Int Rpt
PGYNFRFAKYY P2X4_HUMAN 290 34 -
PGYNFRFAKYY P2X4_RAT 290 34 -
SGYNFRFARYY P2X5_HUMAN 291 33 -
SGYNFRFARYY P2X5_RAT 291 33 -
SGYNFRFAKYY P2X2_RAT 285 32 -
SGYNFRFAKYY P2X2_CAVPO 293 32 -
PGYNFRFAKYY P2X3_HUMAN 276 34 -
PGYNFRFAKYY P2X3_RAT 276 34 -
PGFNFRFARHF P2X1_MOUSE 287 31 -
PGFNFRFARHF P2X1_HUMAN 287 31 -
PGFNFRFARHF P2X1_RAT 287 31 -
PGYNFRYAKYY P2X7_RAT 289 34 -
RGYNFRTANYW P2X6_RAT 284 25 -
PGYNFRYAKYY P2X7_HUMAN 289 34 -
KGYNFRTANHW P2X6_MOUSE 284 25 -
KSYNFRTATHW P2X6_HUMAN 282 25 -

Motif 5 width=15
Element Seqn Id St Int Rpt
TLIKAYGIRFDIIVF P2X4_HUMAN 310 9 -
TLTKAYGIRFDIIVF P2X4_RAT 310 9 -
TLMKAYGIRFDVMVN P2X5_HUMAN 311 9 -
DLMKAYGIRFDVIVN P2X5_RAT 311 9 -
TLIKAYGIRIDVIVH P2X2_RAT 305 9 -
TLIKAYGIRIDVIVH P2X2_CAVPO 312 8 -
TLLKAFGIRFDVLVY P2X3_HUMAN 296 9 -
TLLKAFGIRFDVLVY P2X3_RAT 296 9 -
HLFKVFGIRFDILVD P2X1_MOUSE 306 8 -
HLFKVFGIRFDILVD P2X1_HUMAN 306 8 -
HLFKVFGIHFDILVD P2X1_RAT 306 8 -
TLIKAFGVRFDILVF P2X7_RAT 308 8 -
SLLKLYGIRFDILVT P2X6_RAT 304 9 -
TLIKVFGIRFDILVF P2X7_HUMAN 308 8 -
SLLKLYGIRFDILVT P2X6_MOUSE 304 9 -
TLLKLYGIRFDILVT P2X6_HUMAN 302 9 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
RIWDVADYV P2X4_HUMAN 82 82 -
RIWDVADYV P2X4_RAT 82 82 -
RIWDVADYV Q9Z256 82 82 -
RIWDVADYV Q9Z257 82 82 -
RIWDVADYV Q9YI70 82 82 -
RIWDVADYV O75540 84 84 -
RIWDVADYV P2X5_HUMAN 84 84 -
RLWDVADFV P2X5_RAT 84 84 -
KVWDVEEYV P2X2_RAT 79 79 -
KVWDVEEYV O54869 91 91 -
KVWDVEEYV P2X2_CAVPO 87 87 -
RVMDVSDYV P2X3_HUMAN 73 73 -
RVMDVSDYV P2X3_RAT 73 73 -
QVWDVADYV P2X1_MOUSE 83 83 -
QVWDVADYV P2X1_HUMAN 83 83 -
SIFDTADYT Q9Z1M0 86 86 -
QVWDVADYV P2X1_RAT 83 83 -
GIFDTADYT P2X7_RAT 86 86 -
RLWDVADFV P2X6_RAT 85 85 -
SVFDTADYT P2X7_HUMAN 86 86 -
RLWDVADFV P2X6_MOUSE 85 85 -
RLWDVADFV P2X6_HUMAN 83 83 -
KVWDVEEYV O70399 87 87 -

Motif 2 width=12
Element Seqn Id St Int Rpt
KTCEVAAWCPVE P2X4_HUMAN 157 66 -
KTCEVAAWCPVE P2X4_RAT 157 66 -
KTCEVAAWCPVE Q9Z256 157 66 -
KTCEVAAWCPVE Q9Z257 157 66 -
KTCEVFAWCPVE Q9YI70 157 66 -
GTCEIFAWCPLE O75540 137 44 -
GTCEIFAWCPLE P2X5_HUMAN 161 68 -
GTCEIFAWCPVE P2X5_RAT 161 68 -
KTCEVSAWCPVE P2X2_RAT 156 68 -
RTCEVSAWCPVE O54869 168 68 -
KTCEVSGWCPVE P2X2_CAVPO 164 68 -
RTCEIQGWCPTE P2X3_HUMAN 145 63 -
RTCEIQGWCPTE P2X3_RAT 145 63 -
KTCEIFGWCPVE P2X1_MOUSE 157 65 -
KTCEIFGWCPVE P2X1_HUMAN 157 65 -
KTCEVSAWCPTE Q9Z1M0 160 65 -
KTCEIFGWCPVE P2X1_RAT 157 65 -
KTCEIFAWCPAE P2X7_RAT 160 65 -
RTCEIWSWCPVE P2X6_RAT 161 67 -
KTCEVSAWCPIE P2X7_HUMAN 160 65 -
RTCEIWSWCPVE P2X6_MOUSE 161 67 -
RTCEIWSWCPVE P2X6_HUMAN 159 67 -
KTCEVSGWCPVE O70399 164 68 -

Motif 3 width=13
Element Seqn Id St Int Rpt
FQDMAVEGGIMGI P2X4_HUMAN 243 74 -
FQEMAVEGGIMGI P2X4_RAT 243 74 -
FQEMAVEGGIMGI Q9Z256 216 47 -
FQEMAVEGGIMGI Q9Z257 243 74 -
FQEMAVEGGVMGL Q9YI70 243 74 -
FQDIALEGGVIGI O75540 220 71 -
FQDIALRGGVIGI P2X5_HUMAN 245 72 -
FQDIALKGGVIGI P2X5_RAT 245 72 -
FTELAHKGGVIGV P2X2_RAT 240 72 -
FTELAHKGGVIGV O54869 252 72 -
FTELAHRGGVIGV P2X2_CAVPO 248 72 -
FAKLARTGGVLGI P2X3_HUMAN 229 72 -
FAKLARTGGVLGI P2X3_RAT 229 72 -
FRSLAEKGGVVGI P2X1_MOUSE 243 74 -
FSTLAEKGGVVGI P2X1_HUMAN 243 74 -
FTEVAVQGGIMGI Q9Z1M0 242 70 -
FRSLAEKGGVVGI P2X1_RAT 243 74 -
FTEVAVQGGIMGI P2X7_RAT 242 70 -
FEDLALLGGAVGI P2X6_RAT 246 73 -
FSDVAIQGGIMGI P2X7_HUMAN 242 70 -
FEDLALLGGAVGI P2X6_MOUSE 246 73 -
FEDLALLGGSVGI P2X6_HUMAN 244 73 -
FTELAHRGGVIGV O70399 248 72 -

Motif 4 width=11
Element Seqn Id St Int Rpt
PGYNFRFAKYY P2X4_HUMAN 290 34 -
PGYNFRFAKYY P2X4_RAT 290 34 -
PGYNFRFAKYY Q9Z256 263 34 -
PGYNFRFAKYY Q9Z257 290 34 -
PGYNFRFAKYY Q9YI70 290 34 -
SGYNFRFARYY O75540 266 33 -
SGYNFRFARYY P2X5_HUMAN 291 33 -
SGYNFRFARYY P2X5_RAT 291 33 -
SGYNFRFAKYY P2X2_RAT 285 32 -
SGYNFRFAKYY O54869 297 32 -
SGYNFRFAKYY P2X2_CAVPO 293 32 -
PGYNFRFAKYY P2X3_HUMAN 276 34 -
PGYNFRFAKYY P2X3_RAT 276 34 -
PGFNFRFARHF P2X1_MOUSE 287 31 -
PGFNFRFARHF P2X1_HUMAN 287 31 -
PGYNFRYAKYY Q9Z1M0 289 34 -
PGFNFRFARHF P2X1_RAT 287 31 -
PGYNFRYAKYY P2X7_RAT 289 34 -
RGYNFRTANYW P2X6_RAT 284 25 -
PGYNFRYAKYY P2X7_HUMAN 289 34 -
KGYNFRTANHW P2X6_MOUSE 284 25 -
KSYNFRTATHW P2X6_HUMAN 282 25 -
RSLHPRFAKYY O70399 320 59 -

Motif 5 width=15
Element Seqn Id St Int Rpt
TLIKAYGIRFDIIVF P2X4_HUMAN 310 9 -
TLTKAYGIRFDIIVF P2X4_RAT 310 9 -
TLTKAYGIRFDIIVF Q9Z256 283 9 -
TLTKAYGIRFDIIVF Q9Z257 310 9 -
TLIKAYGIRLDIIVF Q9YI70 310 9 -
TLMKAYGIRFDVMVN O75540 286 9 -
TLMKAYGIRFDVMVN P2X5_HUMAN 311 9 -
DLMKAYGIRFDVIVN P2X5_RAT 311 9 -
TLIKAYGIRIDVIVH P2X2_RAT 305 9 -
TLIKAYGIRIDVIVH O54869 317 9 -
TLIKAYGIRIDVIVH P2X2_CAVPO 312 8 -
TLLKAFGIRFDVLVY P2X3_HUMAN 296 9 -
TLLKAFGIRFDVLVY P2X3_RAT 296 9 -
HLFKVFGIRFDILVD P2X1_MOUSE 306 8 -
HLFKVFGIRFDILVD P2X1_HUMAN 306 8 -
TLIKAFGIRFDILVF Q9Z1M0 308 8 -
HLFKVFGIHFDILVD P2X1_RAT 306 8 -
TLIKAFGVRFDILVF P2X7_RAT 308 8 -
SLLKLYGIRFDILVT P2X6_RAT 304 9 -
TLIKVFGIRFDILVF P2X7_HUMAN 308 8 -
SLLKLYGIRFDILVT P2X6_MOUSE 304 9 -
TLLKLYGIRFDILVT P2X6_HUMAN 302 9 -
TLIKAYGIRIDVIVH O70399 339 8 -