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PR01277

Identifier
CHANNELTSX  [View Relations]  [View Alignment]  
Accession
PR01277
No. of Motifs
8
Creation Date
17-FEB-2000
Title
Nucleoside-specific channel-forming protein Tsx signature
Database References
Literature References
1. BREMER E., MIDDENDORF A., MARTINUSSEN J., VALENTIN-HANSEN P.
Analysis of the tsx gene, which encodes a nucleoside-specific channel-
forming protein (Tsx) in the outer membrane of Escherichia coli.
GENE 96 59-65 (1990).


Documentation
The tsx gene of Escherichia coli encodes an outer membrane protein, Tsx,
which constitutes the receptor for colicin K and bacteriophage T6, and 
functions as a substrate-specific channel for nucleosides and deoxy-
nucleosides [1]. The protein contains 294 amino acids, the first 22 of 
which are characteristic of a bacterial signal sequence peptide [1]. The
putative mature form of Tsx contains 272 residues with a calculated Mr of
31418. The Tsx sequence shows an even distribution of charged residues
and lacks extensive hydrophobic stretches [1]. Tsx shows no significant
similarities to the channel-forming proteins OmpC, OmpF, PhoE and LamB
from the E.coli outer membrane.  
 
CHANNELTSX is an 8-element fingerprint that provides a signature for the
nucleoside-specific channel-forming protein Tsx. The fingerprint was derived
from an initial alignment of 4 sequences: the motifs were drawn from 
conserved regions spanning the full alignment length. A single iteration
on SPTR37_10f was required to reach convergence, no further sequences being
identified beyond the starting set.
Summary Information
4 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
844444444
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
TSX_ECOLI     TSX_ENTAE     TSX_KLEPN     TSX_SALTY     
Sequence Titles
TSX_ECOLI   NUCLEOSIDE-SPECIFIC CHANNEL-FORMING PROTEIN TSX PRECURSOR - ESCHERICHIA COLI. 
TSX_ENTAE NUCLEOSIDE-SPECIFIC CHANNEL-FORMING PROTEIN TSX PRECURSOR - ENTEROBACTER AEROGEN
TSX_KLEPN NUCLEOSIDE-SPECIFIC CHANNEL-FORMING PROTEIN TSX PRECURSOR - KLEBSIELLA PNEUMONIA
TSX_SALTY NUCLEOSIDE-SPECIFIC CHANNEL-FORMING PROTEIN TSX PRECURSOR - SALMONELLA TYPHIMURI
Scan History
SPTR37_10f 1  50   NSINGLE    
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
PQYLSDWWHQSVNVVGSYHTRFG TSX_KLEPN 28 28 -
PQYLSDWWHQSVNVVGSYHTRFG TSX_ENTAE 28 28 -
PQYLSDWWHQSVNVVGSYHTRFG TSX_ECOLI 28 28 -
PQYLSDWWHQSVNVVGSYHTRFS TSX_SALTY 28 28 -

Motif 2 width=25
Element Seqn Id St Int Rpt
NDTYLEYEAFAKKDWFDFYGYIDAP TSX_KLEPN 55 4 -
NDTYLEYEAFAKKDWFDFYGYIDAP TSX_ENTAE 55 4 -
NDTYLEYEAFAKKDWFDFYGYADAP TSX_ECOLI 55 4 -
NDVYLEYEAFAKKDWFDFYGYIDIP TSX_SALTY 55 4 -

Motif 3 width=22
Element Seqn Id St Int Rpt
KGIWNKGSPLFMEIEPRFSIDK TSX_KLEPN 89 9 -
KGIWNKGSPLFMEIEPRFSIDK TSX_ENTAE 89 9 -
KGIWNHGSPLFMEIEPRFSIDK TSX_ECOLI 89 9 -
KGIWSDGSPLFMEIEPRFSIDK TSX_SALTY 90 10 -

Motif 4 width=21
Element Seqn Id St Int Rpt
DLSFGPFKEWYFANNYIYDMG TSX_KLEPN 115 4 -
DLSFGPFKEWYFANNYIYDMG TSX_ENTAE 115 4 -
DLSFGPFKEWYFANNYIYDMG TSX_ECOLI 115 4 -
DLSFGPFKEWYFANNYIYDMG TSX_SALTY 116 4 -

Motif 5 width=26
Element Seqn Id St Int Rpt
GTDIDTGLPMSLSLNVYAKYQWQNYG TSX_KLEPN 150 14 -
GTDIDTGLPMSLSLNIYAKYQWQNYG TSX_ENTAE 150 14 -
GTDIDTGLPMSLSMNVYAKYQWQNYG TSX_ECOLI 150 14 -
GTDIDTGLPMGLSLNVYAKYQWQNYG TSX_SALTY 151 14 -

Motif 6 width=24
Element Seqn Id St Int Rpt
WDGYRFKVKYFVPLTDLWGGSLSY TSX_KLEPN 182 6 -
WDAYRFKVKYFVPLTDLWGGSLSY TSX_ENTAE 182 6 -
WDGYRFKIKYFVPITDLWGGQLSY TSX_ECOLI 182 6 -
WDGYRFKVKYFVPITDLWGGKLSY TSX_SALTY 183 6 -

Motif 7 width=20
Element Seqn Id St Int Rpt
HILALNYAHWHYSIVARYFH TSX_KLEPN 240 34 -
HILALNYAHWHYSIVARYFH TSX_ENTAE 240 34 -
HILALNYDHWHYSVVARYWH TSX_ECOLI 240 34 -
HILALNYDHWHYSVVARYFH TSX_SALTY 233 26 -

Motif 8 width=24
Element Seqn Id St Int Rpt
LNFGDGPFSVRSTGWGGYFVVGYN TSX_KLEPN 270 10 -
LNFGDGDFSVRSTGWGGYFVVGYN TSX_ENTAE 270 10 -
LNFGNGNFNVRSTGWGGYLVVGYN TSX_ECOLI 270 10 -
LNWGDGDFSAKSTGWGGYLVVGYN TSX_SALTY 263 10 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
PQYLSDWWHQSVNVVGSYHTRFG TSX_KLEPN 28 28 -
PQYLSDWWHQSVNVVGSYHTRFG TSX_ENTAE 28 28 -
PQYLSDWWHQSVNVVGSYHTRFG TSX_ECOLI 28 28 -
PQYLSDWWHQSVNVVGSYHTRFS TSX_SALTY 28 28 -

Motif 2 width=25
Element Seqn Id St Int Rpt
NDTYLEYEAFAKKDWFDFYGYIDAP TSX_KLEPN 55 4 -
NDTYLEYEAFAKKDWFDFYGYIDAP TSX_ENTAE 55 4 -
NDTYLEYEAFAKKDWFDFYGYADAP TSX_ECOLI 55 4 -
NDVYLEYEAFAKKDWFDFYGYIDIP TSX_SALTY 55 4 -

Motif 3 width=22
Element Seqn Id St Int Rpt
KGIWNKGSPLFMEIEPRFSIDK TSX_KLEPN 89 9 -
KGIWNKGSPLFMEIEPRFSIDK TSX_ENTAE 89 9 -
KGIWNHGSPLFMEIEPRFSIDK TSX_ECOLI 89 9 -
KGIWSDGSPLFMEIEPRFSIDK TSX_SALTY 90 10 -

Motif 4 width=21
Element Seqn Id St Int Rpt
DLSFGPFKEWYFANNYIYDMG TSX_KLEPN 115 4 -
DLSFGPFKEWYFANNYIYDMG TSX_ENTAE 115 4 -
DLSFGPFKEWYFANNYIYDMG TSX_ECOLI 115 4 -
DLSFGPFKEWYFANNYIYDMG TSX_SALTY 116 4 -

Motif 5 width=26
Element Seqn Id St Int Rpt
GTDIDTGLPMSLSLNVYAKYQWQNYG TSX_KLEPN 150 14 -
GTDIDTGLPMSLSLNIYAKYQWQNYG TSX_ENTAE 150 14 -
GTDIDTGLPMSLSMNVYAKYQWQNYG TSX_ECOLI 150 14 -
GTDIDTGLPMGLSLNVYAKYQWQNYG TSX_SALTY 151 14 -

Motif 6 width=24
Element Seqn Id St Int Rpt
WDGYRFKVKYFVPLTDLWGGSLSY TSX_KLEPN 182 6 -
WDAYRFKVKYFVPLTDLWGGSLSY TSX_ENTAE 182 6 -
WDGYRFKIKYFVPITDLWGGQLSY TSX_ECOLI 182 6 -
WDGYRFKVKYFVPITDLWGGKLSY TSX_SALTY 183 6 -

Motif 7 width=20
Element Seqn Id St Int Rpt
HILALNYAHWHYSIVARYFH TSX_KLEPN 240 34 -
HILALNYAHWHYSIVARYFH TSX_ENTAE 240 34 -
HILALNYDHWHYSVVARYWH TSX_ECOLI 240 34 -
HILALNYDHWHYSVVARYFH TSX_SALTY 233 26 -

Motif 8 width=24
Element Seqn Id St Int Rpt
LNFGDGPFSVRSTGWGGYFVVGYN TSX_KLEPN 270 10 -
LNFGDGDFSVRSTGWGGYFVVGYN TSX_ENTAE 270 10 -
LNFGNGNFNVRSTGWGGYLVVGYN TSX_ECOLI 270 10 -
LNWGDGDFSAKSTGWGGYLVVGYN TSX_SALTY 263 10 -