SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01262

Identifier
INNEXIN  [View Relations]  [View Alignment]  
Accession
PR01262
No. of Motifs
6
Creation Date
20-OCT-1999
Title
Innexin signature
Database References

PFAM; PF00876 Innexin
PRODOM; PD002074
INTERPRO; IPR000990
Literature References
1. PHELAN, P., BACON, J.P., DAVIES, J.A., STEBBINGS, L.A., TODMAN, M.G.,
AVERY, L., BAINES, R.A., BARNES, T.M., FORD, C., HEKIMI, S., LEE, R.,
SHAW, J.E., STARICH, T.A., CURTIN, K.D., SUN, Y. AND WYMAN, R.J.
Innexins: a family of invertebrate gap-junction proteins.
TRENDS GENET. 14 348-349 (1998).
 
2. CURTIN, K.D., ZHANG, Z. AND WYMAN, R.J.
Drosophila has several genes for gap junction proteins.
GENE 232 191-201 (1999).

Documentation
Innexins are an invertebrate-specific family of gap junction-forming
proteins. They bear no sequence similarity to the well-characterised
vertebrate gap junction channel-forming molecules (connexins), but share a
similar predicted membrane topology of four transmembrane (TM) domains, with
intercellular N- and C-termini [1].
 
The innexins were initially identified by genetic screens in both C.elegans
and Drosophila species. When their genes were disrupted by mutation, a 
number of abnormal phenotypes were noted. Mutation of C.elegans unc-7 leads
to an uncoordinated phenotype, while eat-5 mutations disrupt pharyngeal 
pumping (a process involved in feeding). Drosophila passover (pas) mutation 
leads to individuals that are unable to fly properly, and have impaired 
escape responses [2]. Further experiments have demonstrated that pas
mutation inactivates electrical synapses between neurones that are involved 
in the neural circuits responsible for these behaviours.
 
To date, 5 fly innexins have been identified, together with more than 25
from worm species, most of the latter arising from the C.elegans
sequencing project.
 
INNEXIN is a 6-element fingerprint that provides a signature for innexins.
The fingerprint was derived from an initial alignment of 14 sequences: the
motifs were drawn from conserved regions spanning the first 2/3 of the
alignment - motif 1 encodes the C-terminal half of the first putative TM
domain; motif 2 lies on the first extracellular loop; motifs 3 and 4 encode
most of the second and third TM domains, respectively; motif 5 lies on the 
second extracellular loop; and motif 6 spans the end of the second 
extracellular loop and the start of the fourth TM domain. Two iterations on
SPTR37_10f were required to reach convergence, at which point a true set 
comprising 25 sequences was identified. Two partial matches were also
found, O61788 and P91827, both of which have arisen from the C.elegans
sequencing project and have been noted to be similar to functionally-
characterised innexins.
Summary Information
  25 codes involving  6 elements
1 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6252525252525
5111011
4100111
3000000
2000000
123456
True Positives
O01393        O01634        O44887        O61715        
O61786 O61787 O61813 O61966
O62136 OGRE_DROME Q17394 Q18049
Q18504 Q19139 Q19746 Q21123
Q21965 Q22549 Q23027 Q23157
Q23593 Q23594 Q27295 SHAK_DROME
UNC7_CAEEL
True Positive Partials
Codes involving 5 elements
O61788
Codes involving 4 elements
P91827
Sequence Titles
O01393      UNC-9 - CAENORHABDITIS ELEGANS.               
O01634 SIMILAR TO C. ELEGANS UNC-7 - CAENORHABDITIS ELEGANS.
O44887 Y8G1A.2 PROTEIN - CAENORHABDITIS ELEGANS.
O61715 COSMID T16H5 - CAENORHABDITIS ELEGANS.
O61786 R12E2.9 PROTEIN - CAENORHABDITIS ELEGANS.
O61787 R12E2.5 PROTEIN - CAENORHABDITIS ELEGANS.
O61813 C18H7.2 PROTEIN - CAENORHABDITIS ELEGANS.
O61966 F26D11.10 PROTEIN - CAENORHABDITIS ELEGANS.
O62136 F07A5.1B PROTEIN - CAENORHABDITIS ELEGANS.
OGRE_DROME OGRE LOCUS PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q17394 TRANSMEMBRANE PROTEIN - CAENORHABDITIS ELEGANS.
Q18049 SIMILARITY TO C. ELEGANS UNC-7 PROTEIN - CAENORHABDITIS ELEGANS.
Q18504 C36H8.2 PROTEIN - CAENORHABDITIS ELEGANS.
Q19139 F07A5.1A PROTEIN - CAENORHABDITIS ELEGANS.
Q19746 UNC-7 PROTEIN - CAENORHABDITIS ELEGANS.
Q21123 SIMILAR TO C. ELEGANS UNC-7 - CAENORHABDITIS ELEGANS.
Q21965 R12H7.1 PROTEIN - CAENORHABDITIS ELEGANS.
Q22549 SIMILAR TO C. ELEGANS UNC-7 AND DROSOPHILA OGRE AND SHAKING-B PROTEINS - CAENORH
Q23027 SIMILARITY TO C. ELEGANS UNC-7 - CAENORHABDITIS ELEGANS.
Q23157 W04D2.3 PROTEIN - CAENORHABDITIS ELEGANS.
Q23593 ZK792.2 PROTEIN - CAENORHABDITIS ELEGANS.
Q23594 ZK792.3 PROTEIN - CAENORHABDITIS ELEGANS.
Q27295 F13G3.8 PROTEIN - CAENORHABDITIS ELEGANS.
SHAK_DROME SHAKING-B PROTEIN PRECURSOR (PASSOVER PROTEIN) - DROSOPHILA MELANOGASTER (FRUIT
UNC7_CAEEL UNC-7 PROTEIN - CAENORHABDITIS ELEGANS.

O61788 R12E2.4 PROTEIN - CAENORHABDITIS ELEGANS.

P91827 T23H4.1 PROTEIN - CAENORHABDITIS ELEGANS.
Scan History
SPTR37_10f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
SAKQYVGFPIQC O01393 44 44 -
SAKQYVGFPIQC UNC7_CAEEL 162 162 -
SCKQYVGSAIQC Q19746 56 56 -
AASQYVGRPIQC Q23027 42 42 -
SFKQFGGRPIEC Q23157 43 43 -
SFKQFGGKPVEC Q22549 42 42 -
SWKQFGGTPIEC Q21123 42 42 -
TGWSFVGSPIDC O01634 40 40 -
TARQYVGSPLQC Q27295 40 40 -
SAKTYVGSAMEC Q23593 183 183 -
TTRQYVGNPIDC SHAK_DROME 31 31 -
LSSHFIGDPITC Q18504 47 47 -
TATQYVGQPISC OGRE_DROME 43 43 -
GAKQHFGNPIDC Q19139 44 44 -

Motif 2 width=10
Element Seqn Id St Int Rpt
YCWVQNTYFL O01393 72 16 -
YCWVQNTYWV UNC7_CAEEL 190 16 -
YCFIQNTFFI Q19746 84 16 -
YCFIKGTYFL Q23027 70 16 -
YCWSQDTYFV Q23157 71 16 -
YCWASDTYYV Q22549 70 16 -
YCWAQPTYFI Q21123 70 16 -
YCYVQNTFFV O01634 68 16 -
YCFVYNTYWV Q27295 68 16 -
YCFLKDTYFY Q23593 211 16 -
YCWIHSTYTV SHAK_DROME 56 13 -
YCFVHGTYFV Q18504 75 16 -
FCWIHSTFTM OGRE_DROME 67 12 -
FCLFYGTFRY Q19139 74 18 -

Motif 3 width=23
Element Seqn Id St Int Rpt
YYQWVPFVLALEALLFYVPTIVW O01393 101 19 -
YYQWVPFILAIEALLFYVPCILW UNC7_CAEEL 219 19 -
YYQWVPIVLAIQAFMFYLPSWIW Q19746 113 19 -
YYQWIPIVLVLQAFLFYLPSIIW Q23027 106 26 -
YYQWVPFFLLLQAAFFRAPSYLW Q23157 103 22 -
YYQWVPFFLLLEAACFRLPSLLW Q22549 100 20 -
YYQWMSFFLLFEAACFRLPCFIW Q21123 121 41 -
YYQWVPFILALQAMLFYFPVVIW O01634 111 33 -
YYQWAPFIMAIEAAFFYLPVIFW Q27295 97 19 -
YYQWSSMYLAVAGIAFMIPKFLW Q23593 241 20 -
YYQWVAFTLFFQAILFYTPRWLW SHAK_DROME 98 32 -
YYQWVPYVFALQAFLFYIPRFIW Q18504 104 19 -
YYQWVCFVLFFQAMACYTPKFLW OGRE_DROME 108 31 -
YYQWVPFFFAFQVCCFLLPFWCW Q19139 103 19 -

Motif 4 width=15
Element Seqn Id St Int Rpt
YISVKILYTVNIVGQ O01393 199 75 -
YIGIKVLYSANVLLQ UNC7_CAEEL 322 80 -
YICIKLMYLANVFVQ Q19746 207 71 -
YLLVKILYLANIVLQ Q23027 200 71 -
YLFTKVLYFLNVFAQ Q23157 202 76 -
YLCTKFFYLANVCLQ Q22549 199 76 -
YFVAKVAFLLNVILQ Q21123 220 76 -
YLFMKALFLINVLFQ O01634 201 67 -
YLVTKLIYMTNSFLQ Q27295 194 74 -
YSVIKILYLVNAIAQ Q23593 325 61 -
YYVCELLALINVIGQ SHAK_DROME 170 49 -
YTLSTVWQAVNAWIQ Q18504 192 65 -
YWACEFLCCINIIVQ OGRE_DROME 180 49 -
YSLTKLFFITNVIIQ Q19139 195 69 -

Motif 5 width=10
Element Seqn Id St Int Rpt
HFPRVTLCDF O01393 247 33 -
MFPRVTLCDF UNC7_CAEEL 370 33 -
VFPRVTLCDF Q19746 255 33 -
IFPRVTMCDF Q23027 248 33 -
YFPRAAVCDF Q23157 251 34 -
MFPRVSLCDF Q22549 248 34 -
VFPRVTLCDF Q21123 275 40 -
NFPRVTMCEY O01634 249 33 -
NFPRIAMCDF Q27295 242 33 -
LFPRVTFCDF Q23593 374 34 -
MFPRMTKCTF SHAK_DROME 221 36 -
HFPRIVHCDF Q18504 241 34 -
VFPRVTKCTF OGRE_DROME 231 36 -
YFPILVGCEY Q19139 265 55 -

Motif 6 width=17
Element Seqn Id St Int Rpt
CVLMINMFNEKIFLFLW O01393 271 14 -
CVLMINMFNEKIFLFLW UNC7_CAEEL 394 14 -
CVLMINMFNEKIYLFIW Q19746 279 14 -
CVIVINMLAEKVYVFFW Q23027 272 14 -
CVLVINIFNEKIFVLLW Q23157 275 14 -
CVLVINIFNEKIFILLW Q22549 272 14 -
CVLLLNLFTEKIFVFLW Q21123 299 14 -
CVLMINMFNEKIFVALW O01634 273 14 -
CVLTLNMFNEKIFLFLY Q27295 266 14 -
CVIGINEFNEKIFLFFW Q23593 399 15 -
CILPLNVVNEKIYIFLW SHAK_DROME 246 15 -
CVLTLNIYYEKLFIFLW Q18504 265 14 -
CILPLNIVNEKTYVFIW OGRE_DROME 256 15 -
CIIPMNVINEKIFIGLY Q19139 290 15 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
SAKQYVGFPIQC O01393 44 44 -
SAKQYVGFPIQC Q21965 44 44 -
SAKQYVGYPIQC Q17394 42 42 -
SAKQYVGYPIQC Q18049 42 42 -
SAKQYVGFPIQC UNC7_CAEEL 162 162 -
SAKQYVGHPIEC O61813 48 48 -
SAKQYVGQPIQC O44887 40 40 -
SCKQYVGSAIQC Q19746 56 56 -
SAKQYGGTPIEC O61715 44 44 -
AASQYVGRPIQC Q23027 42 42 -
SFKQFGGRPIEC Q23157 43 43 -
SFKQFGGKPVEC Q22549 42 42 -
FAKNYVGEPMQC O61787 45 45 -
SWKQFGGTPIEC Q21123 42 42 -
SFKQFGGRPLEC O61966 79 79 -
TGWSFVGSPIDC O01634 40 40 -
TARQYVGSPLQC Q27295 40 40 -
SAKTYVGSAMEC Q23593 183 183 -
TTRQYVGNPIDC SHAK_DROME 31 31 -
SAQSYVGSAMEC Q23594 43 43 -
GYHTYFGRAISC O61786 41 41 -
LSSHFIGDPITC Q18504 47 47 -
TATQYVGQPISC OGRE_DROME 43 43 -
GAKQHFGNPIDC O62136 44 44 -
GAKQHFGNPIDC Q19139 44 44 -

Motif 2 width=10
Element Seqn Id St Int Rpt
YCWVQNTYFL O01393 72 16 -
YCWVQNTYFL Q21965 72 16 -
YCWVENTYYL Q17394 70 16 -
YCWVENTYYL Q18049 70 16 -
YCWVQNTYWV UNC7_CAEEL 190 16 -
YCWVQNTYWV O61813 76 16 -
YCFVQNTYFI O44887 68 16 -
YCFIQNTFFI Q19746 84 16 -
YCWIQNTYWI O61715 72 16 -
YCFIKGTYFL Q23027 70 16 -
YCWSQDTYFV Q23157 71 16 -
YCWASDTYYV Q22549 70 16 -
YCFIENTYFV O61787 73 16 -
YCWAQPTYFI Q21123 70 16 -
YCWAQNTYWV O61966 107 16 -
YCYVQNTFFV O01634 68 16 -
YCFVYNTYWV Q27295 68 16 -
YCFLKDTYFY Q23593 211 16 -
YCWIHSTYTV SHAK_DROME 56 13 -
YCFLKDTYFY Q23594 71 16 -
YCLIENTYYV O61786 69 16 -
YCFVHGTYFV Q18504 75 16 -
FCWIHSTFTM OGRE_DROME 67 12 -
FCLFYGTFRY O62136 74 18 -
FCLFYGTFRY Q19139 74 18 -

Motif 3 width=23
Element Seqn Id St Int Rpt
YYQWVPFVLALEALLFYVPTIVW O01393 101 19 -
YYQWVPFVLALEALLFYVPTIVW Q21965 101 19 -
YYQWVPFVLALEALCFYIPCIMW Q17394 99 19 -
YYQWVPFVLALEALCFYIPCIMW Q18049 99 19 -
YYQWVPFILAIEALLFYVPCILW UNC7_CAEEL 219 19 -
YYQWVPFVLAVAALTFHIPSSVW O61813 105 19 -
YYQWVPFILGLQAILFYLPSLFW O44887 97 19 -
YYQWVPIVLAIQAFMFYLPSWIW Q19746 113 19 -
YYQWVPFILIAEALMFSLPCIFW O61715 101 19 -
YYQWIPIVLVLQAFLFYLPSIIW Q23027 106 26 -
YYQWVPFFLLLQAAFFRAPSYLW Q23157 103 22 -
YYQWVPFFLLLEAACFRLPSLLW Q22549 100 20 -
YYQWVPFLLVIQALFFCVPRAFW O61787 102 19 -
YYQWMSFFLLFEAACFRLPCFIW Q21123 121 41 -
YYQWVPFFLLLQAFLYYIPCLMW O61966 136 19 -
YYQWVPFILALQAMLFYFPVVIW O01634 111 33 -
YYQWAPFIMAIEAAFFYLPVIFW Q27295 97 19 -
YYQWSSMYLAVAGIAFMIPKFLW Q23593 241 20 -
YYQWVAFTLFFQAILFYTPRWLW SHAK_DROME 98 32 -
YYQWSSMYLAVAGIAFMIPKFIW Q23594 101 20 -
YYQWVQFILVFLAFLFYLPYLYW O61786 98 19 -
YYQWVPYVFALQAFLFYIPRFIW Q18504 104 19 -
YYQWVCFVLFFQAMACYTPKFLW OGRE_DROME 108 31 -
YYQWVPFFFAFQVCCFLLPFWCW O62136 103 19 -
YYQWVPFFFAFQVCCFLLPFWCW Q19139 103 19 -

Motif 4 width=15
Element Seqn Id St Int Rpt
YISVKILYTVNIVGQ O01393 199 75 -
YISVKILYTVNIVGQ Q21965 222 98 -
YVFIKMLYLGNVVLQ Q17394 192 70 -
YVFIKMLYLGNVVLQ Q18049 192 70 -
YIGIKVLYSANVLLQ UNC7_CAEEL 322 80 -
YIFIKALHLANVILQ O61813 204 76 -
YLFVKFLYLVQIVFQ O44887 187 67 -
YICIKLMYLANVFVQ Q19746 207 71 -
HSFTKLLYSMNVVAQ O61715 201 77 -
YLLVKILYLANIVLQ Q23027 200 71 -
YLFTKVLYFLNVFAQ Q23157 202 76 -
YLCTKFFYLANVCLQ Q22549 199 76 -
YLVMKLLILLNIVLQ O61787 180 55 -
YFVAKVAFLLNVILQ Q21123 220 76 -
YLATKIMYVGNILTN O61966 236 77 -
YLFMKALFLINVLFQ O01634 201 67 -
YLVTKLIYMTNSFLQ Q27295 194 74 -
YSVIKILYLVNAIAQ Q23593 325 61 -
YYVCELLALINVIGQ SHAK_DROME 170 49 -
YSIVKMLYLIIAIAQ Q23594 185 61 -
YILTKFLFLVNLIAQ O61786 189 68 -
YTLSTVWQAVNAWIQ Q18504 192 65 -
YWACEFLCCINIIVQ OGRE_DROME 180 49 -
YSLTKLFFITNVIIQ O62136 195 69 -
YSLTKLFFITNVIIQ Q19139 195 69 -

Motif 5 width=10
Element Seqn Id St Int Rpt
HFPRVTLCDF O01393 247 33 -
HFPRVTLCDF Q21965 270 33 -
NFPRVTMCDF Q17394 240 33 -
NFPRVTMCDF Q18049 240 33 -
MFPRVTLCDF UNC7_CAEEL 370 33 -
KFPRVTLCDF O61813 253 34 -
HFPRVTMCDF O44887 235 33 -
VFPRVTLCDF Q19746 255 33 -
HFPRVTLCDF O61715 250 34 -
IFPRVTMCDF Q23027 248 33 -
YFPRAAVCDF Q23157 251 34 -
MFPRVSLCDF Q22549 248 34 -
HFPRVSFCDI O61787 228 33 -
VFPRVTLCDF Q21123 275 40 -
NFPRVTLCDF O61966 285 34 -
NFPRVTMCEY O01634 249 33 -
NFPRIAMCDF Q27295 242 33 -
LFPRVTFCDF Q23593 374 34 -
MFPRMTKCTF SHAK_DROME 221 36 -
LFPRVTFCDF Q23594 234 34 -
IFPRQTMCDF O61786 234 30 -
HFPRIVHCDF Q18504 241 34 -
VFPRVTKCTF OGRE_DROME 231 36 -
YFPILVGCEY O62136 265 55 -
YFPILVGCEY Q19139 265 55 -

Motif 6 width=17
Element Seqn Id St Int Rpt
CVLMINMFNEKIFLFLW O01393 271 14 -
CVLMINMFNEKIFLFLW Q21965 294 14 -
CVLMINMFNEKIFLFLW Q17394 264 14 -
CVLMINMFNEKIFLFLW Q18049 264 14 -
CVLMINMFNEKIFLFLW UNC7_CAEEL 394 14 -
CVLVINMLTEKIFIFLW O61813 277 14 -
CVLMINMFNEKVYVFLW O44887 259 14 -
CVLMINMFNEKIYLFIW Q19746 279 14 -
CALLINIINEKVFAFLW O61715 274 14 -
CVIVINMLAEKVYVFFW Q23027 272 14 -
CVLVINIFNEKIFVLLW Q23157 275 14 -
CVLVINIFNEKIFILLW Q22549 272 14 -
CVLMVNMFNEKIFIFLW O61787 252 14 -
CVLLLNLFTEKIFVFLW Q21123 299 14 -
CVLVINIFNEKIFILIW O61966 309 14 -
CVLMINMFNEKIFVALW O01634 273 14 -
CVLTLNMFNEKIFLFLY Q27295 266 14 -
CVIGINEFNEKIFLFFW Q23593 399 15 -
CILPLNVVNEKIYIFLW SHAK_DROME 246 15 -
CLIGINVFNEKIFLFLW Q23594 259 15 -
CVLSMNMLNEKVFLALF O61786 258 14 -
CVLTLNIYYEKLFIFLW Q18504 265 14 -
CILPLNIVNEKTYVFIW OGRE_DROME 256 15 -
CIIPMNVINEKIFIGLY O62136 290 15 -
CIIPMNVINEKIFIGLY Q19139 290 15 -