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PR01253

Identifier
AMPHIPHYSIN2  [View Relations]  [View Alignment]  
Accession
PR01253
No. of Motifs
8
Creation Date
06-DEC-1999
Title
Amphiphysin isoform 2 signature
Database References

PRODOM; PD013907; PD150581
INTERPRO; IPR003023
Literature References
1. WIGGE, P. AND MCMAHON, H.T.
The amphiphysin family of proteins and their role in endocytosis at the
synapse.
TRENDS NEUROSCIENCE 21 339-334 (1998).
 
2. TAKEI, K., SLEPNEV, V.I., HAUCKE, V. AND DE CAMILLI, P.
Functional partnership between amphiphysin and dynamin in clathrin-mediated
endocytosis.
NAT.CELL BIOL. 1 33-39 (1999).

Documentation
Amphiphysins are proteins that are thought to be involved in clathrin-
mediated endocytosis, actin function, and signalling pathways. They are
highly concentrated in nerve terminals, where they may act as linkers
between the clathrin coat and dynamin in the endocytosis of synaptic
vesicles. Such recycling of synaptic vesicles is necessary for
neurotransmission to continue, following neurotransmitter release.
Amphiphysin family members share a similar three-domain organisation: the
N-terminal domain contains six heptad repeats, which are predicted to form
a coiled-coiled structure, thought to be involved in the dimerisation of
amphiphysin molecules; the central region binds the heavy chain of clathrin
and the clathrin adaptor protein AP-2, through distinct sites; and the
C-terminal domain contains a Src-homology-3 (SH3) domain that binds the 
GTPase dynamin and the inositol-5'-phosphatase synaptojanin-1. The 
interactions mediated by both the central and C-terminal domains are
believed to be modulated by proten phosphorylation [1,2].
 
Amphiphysin 2 was the second amphiphysin family member found in mammals.
The gene encoding it has been found to be alternatively spliced. The
various products have been named: BIN-1, Sh3P9, BRAMP-2 and ALP-1. They
have different distribution patterns, with the largest form (~95 kDa) being
expressed solely in the brain, where it shares a very similar (if not
identical) distribution pattern to amphiphysin 1 [1].
 
AMPHIPHYSIN2 is an 8-element fingerprint that provides a signature for
amphiphysin isoform 2. The fingerprint was derived from an initial
alignment of 9 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the amphiphysin isoform 2 but distinguish it from other
isoforms - motifs 1-2 lie near the N-terminus before the putative coiled-
coil domain; motif 3 encodes a portion of the coiled-coil domain; motifs
4-5 lie between the coiled-coil domain and the proline-rich region; motif 6
encodes the start of the proline rich region; motif 7 occurs immediately
prior to the SH3 domain; and motif 8 encodes ~ 1/5 of the SH3 domain. Two
iterations on SPTR37_10f were required to reach convergence, at which point
a true set comprising 17 sequences was identified.
Summary Information
17 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
81717171717171717
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
O00297        O00499        O00545        O08539        
O08839 O43867 O60552 O60553
O60554 O60555 O75514 O75515
O75516 O75517 O75518 Q62434
Q92944
Sequence Titles
O00297      AMPHIPHYSIN II - HOMO SAPIENS (HUMAN).        
O00499 AMPHIPHYSIN II - HOMO SAPIENS (HUMAN).
O00545 AMPHIPHYSIN II - HOMO SAPIENS (HUMAN).
O08539 AMPHIPHYSIN-LIKE (BRAIN AMPHIPHYSIN 2) - MUS MUSCULUS (MOUSE).
O08839 AMPHIPHYSIN - RATTUS NORVEGICUS (RAT).
O43867 PUTATIVE TUMOR SUPPRESSOR - HOMO SAPIENS (HUMAN).
O60552 AMPHIPHYSIN IIB - HOMO SAPIENS (HUMAN).
O60553 AMPHIPHYSIN IIC1 - HOMO SAPIENS (HUMAN).
O60554 AMPHIPHYSIN IIC2 - HOMO SAPIENS (HUMAN).
O60555 AMPHIPHYSIN IID - HOMO SAPIENS (HUMAN).
O75514 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1-10 - HOMO SAPIENS (HUMAN).
O75515 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1-10-13 - HOMO SAPIENS (HUMAN).
O75516 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1-13 - HOMO SAPIENS (HUMAN).
O75517 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1-10+12A - HOMO SAPIENS (HUMAN)
O75518 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1+12A - HOMO SAPIENS (HUMAN).
Q62434 AMPHIPHYSIN-LIKE SH3 DOMAIN-CONTAINING PROTEIN SH3P9 - MUS MUSCULUS (MOUSE).
Q92944 BOX-DEPENDENT MYC-INTERACTING PROTEIN-1 - HOMO SAPIENS (HUMAN).
Scan History
SPTR37_10f 2  60   NSINGLE    
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
EMGSKGVTAGKIAS O00297 3 3 -
EMGSKGVTAGKIAS O60555 3 3 -
EMGSKGVTAGKIAS O60554 3 3 -
EMGSKGVTAGKIAS O60553 3 3 -
EMGSKGVTAGKIAS O60552 3 3 -
EMGSKGVTAGKIAS O00545 3 3 -
EMGSKGVTAGKIAS O00499 3 3 -
EMGSKGVTAGKIAS O08539 3 3 -
EMGSKGVTAGKIAS O08839 3 3 -

Motif 2 width=8
Element Seqn Id St Int Rpt
ANKIAENN O00297 100 83 -
ANKIAENN O60555 100 83 -
ANKIAENN O60554 100 83 -
ANKIAENN O60553 100 83 -
ANKIAENN O60552 100 83 -
ANKIAENN O00545 100 83 -
ANKIAENN O00499 100 83 -
ANKIAENN O08539 100 83 -
ANKIAENN O08839 100 83 -

Motif 3 width=9
Element Seqn Id St Int Rpt
YESLQTAKK O00297 157 49 -
YESLQTAKK O60555 157 49 -
YESLQTAKK O60554 157 49 -
YESLQTAKK O60553 157 49 -
YESLQTAKK O60552 157 49 -
YESLQTAKK O00545 157 49 -
YESLQTAKK O00499 157 49 -
YESLQTAKK O08539 157 49 -
YESLQTAKK O08839 157 49 -

Motif 4 width=14
Element Seqn Id St Int Rpt
EKQHGSNTFTVKAQ O00297 271 105 -
EKQHGSNTFTVKAQ O60555 271 105 -
EKQHGSNTFTVKAQ O60554 240 74 -
EKQHGSNTFTVKAQ O60553 271 105 -
EKQHGSNTFTVKAQ O60552 240 74 -
EKQHGSNTFTVKAQ O00545 240 74 -
EKQHGSNTFTVKAQ O00499 271 105 -
EKQHGSNTFTVKAQ O08539 271 105 -
EKQHGSNTFTVKAQ O08839 271 105 -

Motif 5 width=9
Element Seqn Id St Int Rpt
NAPAKGNKS O00297 288 3 -
NAPAKGNKS O60555 288 3 -
NAPAKGNKS O60554 257 3 -
NAPAKGNKS O60553 288 3 -
NAPAKGNKS O60552 257 3 -
NAPAKGNKS O00545 257 3 -
NAPAKGNKS O00499 288 3 -
NAPEKGNKS O08539 288 3 -
SAPEKGNKS O08839 288 3 -

Motif 6 width=17
Element Seqn Id St Int Rpt
AATPEIRVNHEPEPAGG O00297 305 8 -
AATPEIRVNHEPEPAGG O60555 305 8 -
AATPEIRVNHEPEPAGG O60554 274 8 -
AATPEIRVNHEPEPAGG O60553 305 8 -
AATPEIRVNHEPEPAGG O60552 274 8 -
AATPEIRVNHEPEPAGG O00545 274 8 -
AATPEIRVNHEPEPAGG O00499 305 8 -
AATPEIRVNHEPEPASG O08539 306 9 -
AATPEIRVNHEPEPASG O08839 306 9 -

Motif 7 width=14
Element Seqn Id St Int Rpt
LPAVVVETFPATVN O00297 490 168 -
LPAVVVETFPATVN O60555 447 125 -
LPAVVVETFPATVN O60554 372 81 -
LPAVVVETFPATVN O60553 403 81 -
LPAVVVETFPATVN O60552 415 124 -
LPAVVVETFPATVN O00545 379 88 -
LPAVVVETFPATVN O00499 490 168 -
LPAVVVETFSATVN O08539 485 162 -
LPAVVVETFSATVN O08839 485 162 -

Motif 8 width=10
Element Seqn Id St Int Rpt
NQHKELEKCR O00297 572 68 -
NQHKELEKCR O60555 529 68 -
NQHKELEKCR O60554 454 68 -
NQHKELEKCR O60553 485 68 -
NQHKELEKCR O60552 497 68 -
NQHKELEKCR O00545 461 68 -
NQHKELEKCR O00499 572 68 -
NQHKELEKCR O08539 567 68 -
NQHKELEKCR O08839 567 68 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
EMGSKGVTAGKIAS O00297 3 3 -
EMGSKGVTAGKIAS Q92944 3 3 -
EMGSKGVTAGKIAS O75518 3 3 -
EMGSKGVTAGKIAS O75517 3 3 -
EMGSKGVTAGKIAS O75516 3 3 -
EMGSKGVTAGKIAS O75515 3 3 -
EMGSKGVTAGKIAS O75514 3 3 -
EMGSKGVTAGKIAS O60555 3 3 -
EMGSKGVTAGKIAS O60554 3 3 -
EMGSKGVTAGKIAS O60553 3 3 -
EMGSKGVTAGKIAS O60552 3 3 -
EMGSKGVTAGKIAS O00545 3 3 -
EMGSKGVTAGKIAS O00499 3 3 -
EMGSKGVTAGKIAS O43867 3 3 -
EMGSKGVTAGKIAS O08539 3 3 -
EMGSKGVTAGKIAS Q62434 3 3 -
EMGSKGVTAGKIAS O08839 3 3 -

Motif 2 width=8
Element Seqn Id St Int Rpt
ANKIAENN O00297 100 83 -
ANKIAENN Q92944 100 83 -
ANKIAENN O75518 100 83 -
ANKIAENN O75517 100 83 -
ANKIAENN O75516 100 83 -
ANKIAENN O75515 100 83 -
ANKIAENN O75514 100 83 -
ANKIAENN O60555 100 83 -
ANKIAENN O60554 100 83 -
ANKIAENN O60553 100 83 -
ANKIAENN O60552 100 83 -
ANKIAENN O00545 100 83 -
ANKIAENN O00499 100 83 -
ANKIAENN O43867 100 83 -
ANKIAENN O08539 100 83 -
ANKIAENN Q62434 100 83 -
ANKIAENN O08839 100 83 -

Motif 3 width=9
Element Seqn Id St Int Rpt
YESLQTAKK O00297 157 49 -
YESLQTAKK Q92944 157 49 -
YESLQTAKK O75518 157 49 -
YESLQTAKK O75517 157 49 -
YESLQTAKK O75516 157 49 -
YESLQTAKK O75515 157 49 -
YESLQTAKK O75514 157 49 -
YESLQTAKK O60555 157 49 -
YESLQTAKK O60554 157 49 -
YESLQTAKK O60553 157 49 -
YESLQTAKK O60552 157 49 -
YESLQTAKK O00545 157 49 -
YESLQTAKK O00499 157 49 -
YESLQTAKK O43867 157 49 -
YESLQTAKK O08539 157 49 -
YESLQTAKK Q62434 157 49 -
YESLQTAKK O08839 157 49 -

Motif 4 width=14
Element Seqn Id St Int Rpt
EKQHGSNTFTVKAQ O00297 271 105 -
EKQHGSNTFTVKAQ Q92944 240 74 -
EKQHGSNTFTVKAQ O75518 240 74 -
EKQHGSNTFTVKAQ O75517 240 74 -
EKQHGSNTFTVKAQ O75516 240 74 -
EKQHGSNTFTVKAQ O75515 240 74 -
EKQHGSNTFTVKAQ O75514 240 74 -
EKQHGSNTFTVKAQ O60555 271 105 -
EKQHGSNTFTVKAQ O60554 240 74 -
EKQHGSNTFTVKAQ O60553 271 105 -
EKQHGSNTFTVKAQ O60552 240 74 -
EKQHGSNTFTVKAQ O00545 240 74 -
EKQHGSNTFTVKAQ O00499 271 105 -
EKQHGSNTFTVKAQ O43867 240 74 -
EKQHGSNTFTVKAQ O08539 271 105 -
EKQHGSNTFTVKAQ Q62434 240 74 -
EKQHGSNTFTVKAQ O08839 271 105 -

Motif 5 width=9
Element Seqn Id St Int Rpt
NAPAKGNKS O00297 288 3 -
NAPAKGNKS Q92944 272 18 -
NAPAKGNKS O75518 272 18 -
NAPAKGNKS O75517 257 3 -
NAPAKGNKS O75516 272 18 -
NAPAKGNKS O75515 257 3 -
NAPAKGNKS O75514 257 3 -
NAPAKGNKS O60555 288 3 -
NAPAKGNKS O60554 257 3 -
NAPAKGNKS O60553 288 3 -
NAPAKGNKS O60552 257 3 -
NAPAKGNKS O00545 257 3 -
NAPAKGNKS O00499 288 3 -
NAPAKGNKS O43867 272 18 -
NAPEKGNKS O08539 288 3 -
NAPEKGNKS Q62434 257 3 -
SAPEKGNKS O08839 288 3 -

Motif 6 width=17
Element Seqn Id St Int Rpt
AATPEIRVNHEPEPAGG O00297 305 8 -
AATPEIRVNHEPEPAGG Q92944 289 8 -
AATPEIRVNHEPEPAGG O75518 289 8 -
AATPEIRVNHEPEPAGG O75517 274 8 -
AATPEIRVNHEPEPAGG O75516 289 8 -
AATPEIRVNHEPEPAGG O75515 274 8 -
AATPEIRVNHEPEPAGG O75514 274 8 -
AATPEIRVNHEPEPAGG O60555 305 8 -
AATPEIRVNHEPEPAGG O60554 274 8 -
AATPEIRVNHEPEPAGG O60553 305 8 -
AATPEIRVNHEPEPAGG O60552 274 8 -
AATPEIRVNHEPEPAGG O00545 274 8 -
AATPEIRVNHEPEPAGG O00499 305 8 -
AATPEIRVNHEPEPAGG O43867 289 8 -
AATPEIRVNHEPEPASG O08539 306 9 -
AATPEIRVNHEPEPASG Q62434 275 9 -
AATPEIRVNHEPEPASG O08839 306 9 -

Motif 7 width=14
Element Seqn Id St Int Rpt
LPAVVVETFPATVN O00297 490 168 -
LPAVVVETFPATVN Q92944 351 45 -
LPAVVVETFPATVN O75518 394 88 -
LPAVVVETFPATVN O75517 379 88 -
LPAVVVETFPATVN O75516 321 15 -
LPAVVVETFPATVN O75515 306 15 -
LPAVVVETFPATVN O75514 336 45 -
LPAVVVETFPATVN O60555 447 125 -
LPAVVVETFPATVN O60554 372 81 -
LPAVVVETFPATVN O60553 403 81 -
LPAVVVETFPATVN O60552 415 124 -
LPAVVVETFPATVN O00545 379 88 -
LPAVVVETFPATVN O00499 490 168 -
LPAVVVETFPATVN O43867 394 88 -
LPAVVVETFSATVN O08539 485 162 -
LPAVVVETFSATVN Q62434 331 39 -
LPAVVVETFSATVN O08839 485 162 -

Motif 8 width=10
Element Seqn Id St Int Rpt
NQHKELEKCR O00297 572 68 -
NQHKELEKCR Q92944 433 68 -
NQHKELEKCR O75518 476 68 -
NQHKELEKCR O75517 461 68 -
NQHKELEKCR O75516 403 68 -
NQHKELEKCR O75515 388 68 -
NQHKELEKCR O75514 418 68 -
NQHKELEKCR O60555 529 68 -
NQHKELEKCR O60554 454 68 -
NQHKELEKCR O60553 485 68 -
NQHKELEKCR O60552 497 68 -
NQHKELEKCR O00545 461 68 -
NQHKELEKCR O00499 572 68 -
NQHKKLEKCR O43867 476 68 -
NQHKELEKCR O08539 567 68 -
NQHKELEKCR Q62434 413 68 -
NQHKELEKCR O08839 567 68 -