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PR01242

Identifier
GPROTEINAPLT  [View Relations]  [View Alignment]  
Accession
PR01242
No. of Motifs
8
Creation Date
17-DEC-1999
Title
Plant G-protein alpha subunit signature
Database References
PRINTS; PR00318 GPROTEINA
INTERPRO; IPR002976
PDB; 1GIA
SCOP; 1GIA
Literature References
1. LINDER, M.E. AND GILMAN, A.G.
G proteins.
SCI.AM. 267 56 (1992).
 
2. KAZIRO, Y., ITOH, H., KOZASA, T., NAKAFUKU, M. AND SATOH, T.
Structure and function of signal-transducing GTP-binding proteins.
ANNU.REV.BIOCHEMISTRY 60 349-400 (1991).
 
3. SIMON, M.I., STRATHMANN, M.P. AND GAUTAM, N.
Diversity of G proteins in signal transduction.
SCIENCE 252 802-808 (1993).
 
4. MA, H.
GTP-binding proteins in plants - new members of an old family.
PLANT MOL.BIOL. 26(5) 1611-1636 (1994).

Documentation
Guanine nucleotide binding proteins (G proteins) are a family of membrane-
associated proteins that couple extracellularly-activated integral-membrane
receptors to intracellular effectors, such as ion channels and enzymes that
vary the concentration of second messenger molecules [1-3]. G proteins are
composed of 3 subunits (alpha, beta and gamma) which, in the resting state,
associate as a trimer at the inner face of the plasma membrane. The alpha
subunit has a molecule of guanosine diphosphate (GDP) bound to it:
stimulation of the G protein by an activated receptor leads to its exchange
for GTP (guanosine triphosphate). This results in the separation of the
alpha from the beta and gamma subunits, which always remain tightly
associated as a dimer. Both the alpha and beta-gamma subunits are then able
to interact with effectors, either individually or in a cooperative manner.
The intrinsic GTPase activity of the alpha subunit hydrolyses the bound GTP
to GDP. This returns the alpha subunit to its inactive conformation and
allows it to reassociate with the beta-gamma subunit, thus restoring the
system to its resting state.
 
G protein alpha subunits are 350-400 amino acids in length and have
molecular weights in the range 40-45 kDa. Seventeen distinct types of alpha
subunit have been identified in mammals. These fall into 4 main  groups on
the basis of both sequence similarity and function: alpha-S, alpha-Q,
alpha-I and alpha-12 [3]. Many alpha subunits are substrates for
ADP-ribosylation by cholera or pertussis toxins. They are often N-terminally
acylated, usually with myristate and/or palmitoylate, and these fatty acid
modifications are probably important for membrane association and high-
affinity interactions with other proteins. The atomic structure of the alpha
subunit of the G protein involved in mammalian vision, transducin, has been
elucidated in both GTP- and GDB-bound forms, and shows considerable
similarity in both primary and tertiary structure in the nucleotide-binding
regions to other G proteins, such as p21-ras and EF-Tu.
 
G proteins have also been isolated from a variety of plant species. These
may play a role in signal transduction from hormone receptors [4]. Plant
alpha subunits are highly conserved between species, but share relatively
low sequence similarity with mammalian G proteins. However, the GTP-binding
and hydrolysis regions are well conserved.
 
GPROTEINAPLT is an 8-element fingerprint that provides a signature for 
plant G protein alpha subunits. The fingerprint was derived from an
initial alignment of 4 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise plant alpha subunits but distinguish them from
other G protein alpha subtypes. Two iterations on SPTR37_10f were required
to reach convergence, at which point a true set comprising 11 sequences was
identified. Two partial matches were also found: GBA2_SOYBN and Q43604 are
plant G protein alpha subunits that lack significant matches with motifs 1
and 2 respectively.
Summary Information
  11 codes involving  8 elements
2 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
81111111111111111
711222222
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
GBA1_ARATH    GBA1_LOTJA    GBA1_LUPLU    GBA1_LYCES    
GBA1_ORYSA GBA1_PEA GBA1_SOLTU GBA1_SOYBN
GBA2_PEA O82057 Q40404
True Positive Partials
Codes involving 7 elements
GBA2_SOYBN Q43604
Sequence Titles
GBA1_ARATH  GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP-ALPHA-1) - ARABIDOPSIS TH 
GBA1_LOTJA GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP-ALPHA-1) - LOTUS JAPONICU
GBA1_LUPLU GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP-ALPHA-1) - LUPINUS LUTEUS
GBA1_LYCES GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP-ALPHA-1) - LYCOPERSICON E
GBA1_ORYSA GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP-ALPHA-1) - ORYZA SATIVA (
GBA1_PEA GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP-ALPHA-1) - PISUM SATIVUM
GBA1_SOLTU GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP-ALPHA-1) - SOLANUM TUBERO
GBA1_SOYBN GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNIT (GP-ALPHA-1) - GLYCINE MAX (S
GBA2_PEA GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-2 SUBUNIT (GP-ALPHA-2) - PISUM SATIVUM
O82057 G PROTEIN ALPHA SUBUNIT - SPINACIA OLERACEA (SPINACH).
Q40404 HETEROTRIMERIC G PROTEIN SUBUNIT - NICOTIANA PLUMBAGINIFOLIA (LEADWORT-LEAVED TO

GBA2_SOYBN GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-2 SUBUNIT (GP-ALPHA-2) - GLYCINE MAX (S
Q43604 G PROTEIN ALPHA SUBUNIT - ORYZA SATIVA (RICE).
Scan History
SPTR37_10f 2  15   NSINGLE    
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
RRIEQETKAE GBA1_LYCES 27 27 -
RRIELETKAE GBA1_LUPLU 27 27 -
RRIEQEAKAE GBA1_ARATH 26 26 -
RRIELETKAE GBA1_SOYBN 27 27 -

Motif 2 width=14
Element Seqn Id St Int Rpt
PVIHANVYQTTKIL GBA1_LYCES 77 40 -
PVIHANVFQTIKLL GBA1_LUPLU 77 40 -
PVIHANVYQTIKLL GBA1_ARATH 76 40 -
PVVHANVYQTIKVL GBA1_SOYBN 77 40 -

Motif 3 width=15
Element Seqn Id St Int Rpt
DGSKELAQNELEASK GBA1_LYCES 92 1 -
DGSKELAQNDVDSSK GBA1_LUPLU 92 1 -
DGTKEFAQNETDSAK GBA1_ARATH 91 1 -
DGSKELAQNDFDSSK GBA1_SOYBN 92 1 -

Motif 4 width=15
Element Seqn Id St Int Rpt
IGEKLSEIGGRLDYP GBA1_LYCES 116 9 -
IGEKLSEIGSKLDYP GBA1_LUPLU 116 9 -
IGEKLSEIGGRLDYP GBA1_ARATH 115 9 -
IGQKLSEIGGTLVYP GBA1_SOYBN 116 9 -

Motif 5 width=16
Element Seqn Id St Int Rpt
IQFSPVGENKKSGEVY GBA1_LYCES 200 69 -
IQFSPVGENKRSGEVY GBA1_LUPLU 200 69 -
IQFSPVGENKKSGEVY GBA1_ARATH 199 69 -
IQFSPVGENKRSGEVY GBA1_SOYBN 200 69 -

Motif 6 width=12
Element Seqn Id St Int Rpt
EWVLKQPCFEKT GBA1_LYCES 270 54 -
EWILKQPCFEKT GBA1_LUPLU 270 54 -
DWVLKQPCFEKT GBA1_ARATH 269 54 -
EWVLRQPCFEKT GBA1_SOYBN 270 54 -

Motif 7 width=16
Element Seqn Id St Int Rpt
QEIEHAYEFVKKKFEE GBA1_LYCES 319 37 -
QEIEHAYEFVKKKFEE GBA1_LUPLU 319 37 -
QEIEHAYEFVKKKFEE GBA1_ARATH 318 37 -
QEIEHAYEFVKKKFEE GBA1_SOYBN 320 38 -

Motif 8 width=13
Element Seqn Id St Int Rpt
YFQCTAPDRVDRV GBA1_LYCES 336 1 -
YFQSTAPERVDRV GBA1_LUPLU 336 1 -
YYQNTAPDRVDRV GBA1_ARATH 335 1 -
YFQSTAPDCVDRV GBA1_SOYBN 337 1 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
RRIELETKAE GBA1_LOTJA 27 27 -
RRIEQETKAE GBA1_LYCES 27 27 -
RRIEQETKAD GBA1_SOLTU 27 27 -
RRIEQETKAD Q40404 35 35 -
RRIELETKAE GBA1_PEA 27 27 -
RRIELETKAE GBA1_LUPLU 27 27 -
RRIEQEAKAE GBA1_ARATH 26 26 -
RRIELETKAE GBA1_SOYBN 27 27 -
RRIESETKAE GBA2_PEA 27 27 -
RRIERETIAE O82057 26 26 -
RRILQETKAE GBA1_ORYSA 27 27 -

Motif 2 width=14
Element Seqn Id St Int Rpt
PVIHANVYQTIKLL GBA1_LOTJA 77 40 -
PVIHANVYQTTKIL GBA1_LYCES 77 40 -
PVIHANVYQTIKIL GBA1_SOLTU 77 40 -
PVIHANVYQTIKVL Q40404 85 40 -
PVVHANVYQTIKLL GBA1_PEA 77 40 -
PVIHANVFQTIKLL GBA1_LUPLU 77 40 -
PVIHANVYQTIKLL GBA1_ARATH 76 40 -
PVVHANVYQTIKVL GBA1_SOYBN 77 40 -
PVIFANVYQTIKVL GBA2_PEA 77 40 -
PVIHANVYQTIKLL O82057 76 40 -
SVIHANVYQTIKIL GBA1_ORYSA 77 40 -

Motif 3 width=15
Element Seqn Id St Int Rpt
DGAKELAQNDVDFSK GBA1_LOTJA 92 1 -
DGSKELAQNELEASK GBA1_LYCES 92 1 -
DGSKELAQNELEASK GBA1_SOLTU 92 1 -
DGSKELAQSELEASK Q40404 100 1 -
DGSKEFAQNDVDFSK GBA1_PEA 92 1 -
DGSKELAQNDVDSSK GBA1_LUPLU 92 1 -
DGTKEFAQNETDSAK GBA1_ARATH 91 1 -
DGSKELAQNDFDSSK GBA1_SOYBN 92 1 -
DGAKELAQNDLNSAK GBA2_PEA 92 1 -
DGSKELAQNETDSSK O82057 91 1 -
EGAKELSQVESDSSK GBA1_ORYSA 92 1 -

Motif 4 width=15
Element Seqn Id St Int Rpt
IGEKLSEIGGRLDYP GBA1_LOTJA 116 9 -
IGEKLSEIGGRLDYP GBA1_LYCES 116 9 -
IGEKLSEIGGRLDYP GBA1_SOLTU 116 9 -
IGEKLSEIGGRLDYP Q40404 124 9 -
IGEKLSEIGGRLDYP GBA1_PEA 116 9 -
IGEKLSEIGSKLDYP GBA1_LUPLU 116 9 -
IGEKLSEIGGRLDYP GBA1_ARATH 115 9 -
IGQKLSEIGGTLVYP GBA1_SOYBN 116 9 -
IGEKLSEIGSRLDYP GBA2_PEA 116 9 -
IGDKLSEIGGRLDYP O82057 115 9 -
IGEKLSDIDGRLDYP GBA1_ORYSA 116 9 -

Motif 5 width=16
Element Seqn Id St Int Rpt
IQFSPVGENKKSGEVY GBA1_LOTJA 200 69 -
IQFSPVGENKKSGEVY GBA1_LYCES 200 69 -
IQFSPVGENKKSGEVY GBA1_SOLTU 200 69 -
IQFSPVGENKKSGEVY Q40404 208 69 -
IQFSPVGENKKSGEVY GBA1_PEA 200 69 -
IQFSPVGENKRSGEVY GBA1_LUPLU 200 69 -
IQFSPVGENKKSGEVY GBA1_ARATH 199 69 -
IQFSPVGENKRSGEVY GBA1_SOYBN 200 69 -
IQFSPVGENKRSGEVY GBA2_PEA 200 69 -
IQFSPVGENKKSGEVY O82057 199 69 -
IQFSPVGENKRGGEVY GBA1_ORYSA 200 69 -

Motif 6 width=12
Element Seqn Id St Int Rpt
EWVLKQPCFEKT GBA1_LOTJA 270 54 -
EWVLKQPCFEKT GBA1_LYCES 270 54 -
EWVLKQPCFEKT GBA1_SOLTU 270 54 -
EWVLKQPCFEKT Q40404 258 34 -
EWVLKQQCFEKT GBA1_PEA 270 54 -
EWILKQPCFEKT GBA1_LUPLU 270 54 -
DWVLKQPCFEKT GBA1_ARATH 269 54 -
EWVLRQPCFEKT GBA1_SOYBN 270 54 -
EWILKQPCFEKT GBA2_PEA 270 54 -
EWVLKQRCFERT O82057 269 54 -
DWVLKQRCFEKT GBA1_ORYSA 270 54 -

Motif 7 width=16
Element Seqn Id St Int Rpt
QEIEHAYEFVKKKFEE GBA1_LOTJA 319 37 -
QEIEHAYEFVKKKFEE GBA1_LYCES 319 37 -
QEIEHAYEFVKKKFEE GBA1_SOLTU 319 37 -
QEIEHAYEFVKKKFEE Q40404 307 37 -
QEIEHAYEFVKKKFEE GBA1_PEA 319 37 -
QEIEHAYEFVKKKFEE GBA1_LUPLU 319 37 -
QEIEHAYEFVKKKFEE GBA1_ARATH 318 37 -
QEIEHAYEFVKKKFEE GBA1_SOYBN 320 38 -
QEIEHAYEFVKKKFEE GBA2_PEA 319 37 -
QEIEHTYEFVKKKFEE O82057 318 37 -
QEVEHAYEFVKKKFEE GBA1_ORYSA 319 37 -

Motif 8 width=13
Element Seqn Id St Int Rpt
YFQNTAPDRVDRV GBA1_LOTJA 336 1 -
YFQCTAPDRVDRV GBA1_LYCES 336 1 -
YFQCTAPDRVDRV GBA1_SOLTU 336 1 -
YFQCTAPDRVDRV Q40404 324 1 -
YFQSTAPDSVDRV GBA1_PEA 336 1 -
YFQSTAPERVDRV GBA1_LUPLU 336 1 -
YYQNTAPDRVDRV GBA1_ARATH 335 1 -
YFQSTAPDCVDRV GBA1_SOYBN 337 1 -
YFQSSAPDRVDRV GBA2_PEA 336 1 -
YYQCTAPDRVDRV O82057 335 1 -
YFQSSKPDRVDRV GBA1_ORYSA 336 1 -